3’ Thiol, 3’-Thiol-modifier C6
S-S CPG
Box 1 | Product List
3’ Thiol-Modifier C6 S-S CPG
Cat. No. Amount
AHP-CH-TC60100 100 mg
AHP-CH-TC61000 1000 mg
Name: 3’-Thiol-modifier C6 S-S CPG
Chemical Name: 1-O-Dimethoxytrityl-hexyl-disulfide,1’-succi-
noyl-long chain alkylamino-CPG
Formula: N/A
Use: 3’-Thiol-modifier C6 S-S CPG is used to label a thiol group
at the 3’ end of oligonucleotides.
Purity: 95%+
Diluent: Anhydrous Acetonitrile
Stability in Solution: N/A
Background:
Thiol modifications are important in the production of diagnos-
tic probes. They are use in DNA synthesizers to functionalize
oligonucleotides. The terminal thiol group allows attachment of
an oligo to various ligands, such as biotin, alkaline phosphatase
or fluorescent dyes. Via an iodoacetamide or maleimide link-
age, it allows for the attachment of an oligo to a solid surface.
Thiol modification can be placed at either the 5’ or 3’ end of the
oligonucleotide. For introduction of a thiol group at the 3’-end,
thiol-modifier C6 S-S CPG is available. To introduce a thiol
group at the 5’ end, either 5’-thiol-modifier-C6 or 5’-thiol modi-
fier C6 S-S may be used.
Coupling: All oxidation steps should use 0.02M Iodine solution
to avoid oxidative cleavage of the disulfide linkage.
Deprotection: After proceeding with normal deprotection, the
disulfide can be cleaved at room temperature in 30 minutes with
a buffered 100 mM DTT pH 8.3 - 8.5 solution. It is imporant to
completely remove the reducing agent before using the oligo in
a coupling reaction.
Storage: Freezer storage, -10 to -30°C, dry, dark area
Labeled Oligo Storage: Store labeled oligo in the dark, either
dry or in a neutral aqueous media at -20°C. Do not store crude
fluorescently labeled oligonucleotides using ammonia.
MSDS: www.allelebiotech.com
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