Curriculum Vitae

Zhigang Zhou
2204 Drew Hill Lane
Chapel Hill, NC 27514
(734)-276-0629
zz83a330@westpost.net

Education
*Ph.D., Shanghai Medical University, Shanghai, China 9/1997-7/2000
*M.S. , Henan Medical University, Zhengzhou, China 9/1994-7/1997
*M.D., Henan Medical University, Zhengzhou, China 9/1988-7/1993
Postdoctoral Training
*Postdoctoral fellow, Department of Neurology, University of Michigan, Ann Arbor
, Michigan 07/2004-9/2005
*Postdoctoral fellow, Department of Neurology, University of Pittsburgh, Pitts
burgh, PA 11/2001-06/2004

Professional Experience
Department of Neurology, University of Michigan, MI (9/2005-07/2010)
Research laboratory Specialist Senior
*Conducting experiments to evaluate the effect of gene therapy on the regenerati
on of spinal cord after injury, neuropathic pain and neurodegenerative disease.
*Studying new mechanisms of microglial activation in neuropathic pain, and mech
anisms of cell death after spinal cord injury.
*Independently plan and design experiments with little supervision.
*Independently conduct a variety of molecular cell biology, virology and biochem
istry experiments
*Teach graduate students and laboratory technicians about molecular cell biology
and biochemistry experiments assisting them to troubleshoot daily work
*Assist the Principal Investigator get NIH R01 grant awards and review scientifi
c articles
Department of Neurology, University of Michigan, MI (07/2004-09/2005)
Research fellow
*Expertise in molecular cell biology, virology and biochemistry experiments
*Able to handle multiple experiments in limited time frames and complete tasks w
ithin projected deadlines
*Conduct experiments with patience and accuracy
*Document research results in an accurate and concise manner
*Establish and invent new techniques to improve existing methods for fulfilling
the project's needs and strengthening the compatibility of the entire laboratory
Department of Neurology, University of Pittsburgh, PA (11/2001-06/2004)
Research fellow
*Conducted experiments to study the protective mechanism of HSP27 in stroke.
*evaluate the effect of protein therapy on the stroke. I generated four biolog
ically active fusion proteins (Hsp27, MnSOD, Survivin, xIAP and JBD) containin
g the 11 aa protein transduction domain (PTD) derived from the human immunodefic
iency virus TAT protein, which can pass BBB. In vitro study, I found that Hsp27
protect neuron against cell death through blocking AIF release from mitochondria
. In vivo systemic delivery of Hsp27 fusion protein protects against focal ische
mic injury and inhibits an apoptotic component of ischemic neuronal death after
injected intraperitoneally.
*Plan and design research projects
*Protein purification
*Learn and handle basic molecular biology experiments
*Learn English writing skills for scientific papers
Department of Neurology, Huashan Hospital, Shanghai Medical University, Shangha
i, China (7/2000-11/2001) Attending Physician of Neurology
*Expertise in diagnosing and treatment of patients who have various neurological
diseases.
*As an attending physician, participate in the daily teaching for medical fellow
s, residents, and students to diagnosis and treat patients with neurological di
seases
*Teach internal medicine class (neurological disease section) for medical stude
nts
*Plan and design four clinical III stage trails with department director and ind
ependently summarize the data of these trials
*Teach and supervise graduate students for basic research
*Contributed to the writing of a medical text book (Neurology)
Department of Internal Medicine, The Fifth Affiliated Hospital, Henan Medical U
niversity, Henan, China (7/1993-09/1994) Residency

Special Competencies and Interests

Molecular Biology:
Plasmid/retroviral plasmid construction, stable/transient transfection, Virus in
fection, PCR, RT-PCR, siRNA, HSV shRNA, HSV miRNA, DNA and RNA isolation, situ
hybridization, Southern and Northern transfer.
Virology:
Virus production, purification and virus inactivation.
Cell Biology and Biochemistry:
Cell line/primary cell culture, generate stable/inducible cell line. cell differ
entiation and apoptosis assays, protein kinase activity assay, protein extracti
on and purification, design peptide antibody, antibody testing and purification,
ELISA, immunoprecipitation, GST or His fused protein pulls down, Western blot,
cellular membrane fractionation, immunofluorescent double/trouble staining, immu
nohistochemistry, antibody labeling and pulse-chase, flow cytometry, large scale
protein purification/ Mass spectrometry
Microscope:
Light microscopy, fluorescent/laser confocal microscopy
Animal Models:
Skillfully worked with various animal models of mice and rats: Spinal cord injur
y and pain model.
Computer skills:
Microsoft word, Microsoft Excel, Power Point, Photoshop, Adobe Illustrator, SYST
AT, Endnote, Laser Gene, NCBI BLAST, SMART protein analysis
Membership in Professional Societies
American Society for Biochemistry and Molecular Biology
Society for Neuroscience
Publications
1.Zhou Z, Peng X, Hagshenas J, Insolera R, Fink DJ and Mata M. Transmembrane TNF
* mediated signaling in microglia: Implications for neuropathic pain. Pain. 2010
Jul 5. [Epub ahead of print].
2.Peng XM, Zhou Z, Jianhu, Fink DJ and Mata M. Soluble Nogo receptor down-regul
ates expression of neuronal Nogo-A to enhance axonal regeneration. J Biol Chem.
2010 Jan 22;285(4):2783-95.
3.Zhou Z, Peng X, Insolera R, Fink DJ and Mata M. IL-10 promotes neuronal surviv
al following spinal cord injury. Exp Neurol. 2009 Nov; 220(1): 183-90.
4.Zhou Z, Peng X, Insolera R, Fink DJ and Mata M. Interleukin-10 provides direct
trophic support to neurons. Journal of Neurochemistry. 2009 Sep; 110(5): 1617-2
7.
5.Zhou Z, Peng X, Fink DJ, Mata M. HSV-mediated transfer of artemin overcomes my
elin inhibition to improve outcome after spinal cord injury. Mol Ther. 2009 Jul;
17(7): 1173-9.
6.Zhou Z, Peng X, Hao S, Fink DJ, Mata M. HSV-mediated transfer of interleukin-1
0 reduces inflammatory pain through modulation of membrane tumor necrosis fac
tor alpha in spinal cord microglia. Gene Ther. 2008 Feb;15(3):183-90.
7.Peng XM, Zhou ZG, Glorioso JC, Fink DJ, Mata M. Tumor necrosis factor-alpha co
ntributes to below-level neuropathic pain after spinal cord injury. Ann Neurol.
2006 May;59(5):843-51.
8.Zhang F, Signore AP, Zhou Z, Wang S, Cao G, Chen J. Erythropoietin protects
CA1 neurons against global cerebral ischemia in rat: potential signaling mechani
sms. J Neurosci Res. 2006 May 15;83(7):1241-51.
9.Liou AK, Zhou Z, Pei W, Lim TM, Yin XM, Chen J. BimEL up-regulation potentiate
s AIF translocation and cell death in response to MPTP. FASEB J. 2005 Aug;19(10)
:1350-2.
10.G Cao, AKF Liou, FR. Sharp, Z Zhou, and J Chen. Protein Transduction: a novel
approach to study neurodegeneration and stroke. In Krieglstein J, Klumpp S(Eds)
, pharmacology of cerebral ischemia 2002, 198-212
11.Ren Huiming, Zhou Zhigang, Chen Xiangjun, Huang Jun and Lu Chuanzhen. Compari
son of Protein components in normal and myasthenia gravis skeletal muscles. C
hin J Clin Neurosci 2000, 8(4), 260~262
12.Ren Huiming, Zhou Zhigang, Chen Xiangjun, Huang Jun and Lu Chuanzhen. Analysi
s of protein components of skeletal muscle from the patients with myasthenia gra
vis. Chin J Neurol, 2000,33(4);227-230.
13.Zhou Zhigang, Lu Jiahogn, Ren Huiming and Lu Chuanzhen. The expression of P2
and P9 in myasthenia gravis. Journal of China, 2000, 80(9): 720-722.
14.Zhou Zhigang, Wu Yongqin, Ren Huiming and Lu Chuanzhen. The expression of P1
0 mRNA in the muscle and thymus tissue of MG patient. Chin J Neurol, February 2
000, (33);20-22.
15.Zhou Zhigang, Ren Huimin, Lu Chuanzhen, et al. The expression of gene relat
ed to MG. Acta Pharmacologica Sinica, 1999, 10-12.

Manuscript under revision

1.Zhou Z, Peng X, Fink DJ and Mata M. Neurite extension by dopaminergic cells is
characterized by reduced *-synuclein expression: implications for pathogenesis
of parkinson's disease. PNAS. 2010.
2.Peng X, Zhou Z, Jeeyong Kim , Fink DJ and Mata M. Neuronal NogoA regulates
post-synaptic glutamate receptors. J Biol Chem. 2010
3.Zhou Z, Chattopadhyay M, Mata M and Fink DJ. Vector-mediated knock down of NA
v in DRG reduces pain-related behaviors in rats with painful diabetic neuropathy
. Brain. 2010

Conferences and presentation

1.Zhigang Zhou, Chattopadhyay M, Mata M and Fink DJ. Vector-mediated knock down
of Nav in DRG reduces pain-related behaviors in rats with painful diabetic neur
opathy. Society for Neuroscience Annual Meeting, Chicago, 2009
2.Zhigang Zhou, Xiangmin Peng, Jafar Hagshenas, Ryan Insolera, David J. Fink, an
d Marina Mata. Transmembrane TNF* mediated snalingin microglia: Implications fo
r neuropathic pain. Society for Neuroscience Annual Meeting, Chicago, 2009
3.Zhigang Zhou, Shuanglin Hao, Luis lopez-Santiago, Lori L. Isom, Marina Mata a
nd David J. Fink, and Marina Mata. Society for Neuroscience Annual Meeting, Was
hington DC, 2008
4.Zhigang Zhou, Xiangmin Peng, Ryan Insolera, David J. Fink, and Marina Mata. I
L-10 Prevents Glutamate-Induced Neuronal Death Through Activation of NF-*B. Soci
ety for Neuroscience Annual Meeting, San Diego, 2007
5.Zhigang Zhou, Xiangmin Peng, Shuanglin Hao, David J. Fink, and Marina Mata. IL
-10 reduces inflammatory pain in part by blocking synthesis and release of TNF*
in the spinal cord. Society for Neuroscience Annual Meeting, Atlanta, 2006
6.Zhigang Zhou, Marina Mata, Veliko Puskovic, Kirk Frey, Xia Shao, Michael Kilbo
urn and David J. Fink. Construction of a replication-defective herpes simplex v
irus vector expressing butyrylcholinesterase. Society for Neuroscience Annual Me
eting, Washington DC, 2005
7.Z. Zhou, F. Zhang, W. Pei, G. Cao, K. Liou, F. Guo, W.C. Simon, J. Chen
. Neuroprotection against ischemic neuronal injury by heat shock protein 27 in
volves both caspase-dependent and AIF-dependent mechanisms. Society for Neurosc
ience Annual Meeting, San Diego, 2004
8.Zhou Zhigang, Ren Huimin and Lu Chuanzhen. Studies on genes related to MG.
Sixth National Symposium on Neurology, Wuhan, China, 2000
9.Zhou Zhigang, Ren Huimin and Lu Chuanzhen. A genetic study on myasthenia gr
avis. Symposium of Sino-Americal Neurological Conference; Shanghai, China, 200
0
10.Zhou Zhigang, Ren Huimin and Lu Chuanzhen. Screening and identification of
genes related to myasthenia gravis. Proceedings of the International Symposium
on Advances of Neuroimmunology, Shanghai, China. 1999