Slide lab review

Tahany M. Abu Al - Teen

 Collection of the most important slides in the first 7
labs of oral histology lab

2
5

4
3

1
Primary epithelial band

2
Vestibular lamina
3
Dental lamina
4
Early tooth formation (Bud stage)
5
Condensed ectomesenchymal tissue

*** How to differentiate btwn dental lamina and vestibular

lamina in any section??
 Dental lamina
Goes lingually
Has swelling (condensation)
at it's end where the cells are
packed together
Surrounded by condensed
ectomesenchymal tissue
Vestibular lamina
Goes buccally
Cells have spaces btwn them
till the whole structure
becomes a space
Ectomesenchymal tissue
around vestibular lamina
doesn't condense

Invaginate into condensed

Doesn't invaginate
ectomesenchymal tissue

(Primary epithelial band = PEB, Dental lamina = DL,

Vestibular lamina = VL)

** Primary epithelial band = invagination of oral epithelium

into underlying condensed ectomesenchymal tissue and it
divides there into dental and vestibular laminea

** PEB and DL and VL all these exist in condensed

ectomesenchymal tissue

** Invagination of DL into condensed ectomesenchymal

tissue is responsible for teeth formation and it passes
through 3 stages (based on the shape of the invagination)
and these stages are :

1. Bud stage 2. Cap stage 3. Bell stage

** In this slide the invagination looks like a bud that's why we

call this stage of teeth formation the " Bud stage "

SO >> the bud, cap and bell all these come from DL and they
describe the way in which crowns (nt roots) are formed
 1

3 2

1
DL
2
The cells at the end of DL start to pack together and
prepare themselves to invaginate into ectomesenchymal
tissue to start teeth formation

3
The ectomesenchymal tissue starts to condense around

the end of DL

No invagination has appeared yet

Bud
Stag
e
This slide represents the "Bud Stage" coz the invagination
looks like a bud

Cap
Stage

2
5
3
4

** This slide represents the "Cap stage" coz here the

invagination looks like a cap

1
EEE
2
SR
3
IEE
4
Dental papilla
5
Enamel Knot (transient structure in the center of IEE that
bulges into dental papilla)
 1 2 3 4

1
SR
2
SI
3
mature IEE (preameloblast)
4
undifferentiated ectomesenchymal cells (are going to
become odontoblasts)

** At which stage this slide is??

Notice
1. The presence of SI (2-3 layers of cells over IEE)
2. IEE cells are mature and about to differentiate into
ameloblasts
3. The ectomesenchymal cells aren't differentiated yet
4. NO hard tissue formation has started yet

All these tell that this tooth is in the "Early

"Early Bell Stage"
tage" and it
isn't in the cap stage coz in cap stage we don't have SI and
the IEE cells are slightly columnar, and it isn't in the late bell
stage also coz we don't have any hard tissue formation and
(dentine + enamel) producing cells aren't mature yet
 8

2
7
3

1
EEE
2
Enamel formation
3
Dentine formation
4
SR
5
IEE
6
Cervical loop
7
Area where there's NO hard tissue formation
8
Dental follicle
9
Dental papilla

** At which stage this slide is??

It is at "Late Bell Stage" coz we have hard tissue formation

 5

1
EEE
2
SR
3
Enamel
4
Dentine
5
Dental pulp

This tooth has 2 cusps

Notice these lines that are running obliquely across the
prisms of enamel these are the long incremental lines or
enamel striae (nt all of them reach the surface)

When enamel striae appear like lines running obliquely

across enamel prisms then this is a longitudinal section
across enamel
 The previous slide represents Enamel lamella than runs
through the entire thickness of enamel

Enamel
Enamel spindles
tufts

Scalloped pattern of
EDJ

This slide represents two structures exist at EDJ and these

are Enamel tufts and Enamel spindles

** How to differentiate btwn them in a histological section??

** Enamel spindles = are of unequal length and aren't backed

together and thicker than enamel tufts and they are believed
to be odontoblastic processes

** Enamel tufts = resemble tuft of grass, are approximately of

equal length, very close to each other and backed together
and thinner than enamel spindles
The encircled structure represents enamel prisms
The previous slide represents enamel striae but this is a
cross section of enamel that's why they appear
circumpulpally running

The encircled structure here shows dead tracts of dentine

(dead odontoblastic processes)

1 2 3 4
1
SR
2
SI
3
Ameloblasts
4
Enamel

The encircled structures are Tome's processes

** At which stage of amelogenesis this stage is??

Secretory stage

1 2 3 4 5 6 7 8
1
undifferentiated ectomesenchymal cells (dental papilla)
2
Odontoblast
3
Dentine
4
Enamel
5
Ameloblast
6
Tome's process
7
SI
8
SR

PDL

A = Granular layer of Tome's

B = Cementum

C = Hyaline layer (appears as space)

Arrow head = circumpulpal dentine

Is it coronal or root dentine??

Root dentine (based on the type of the layers)

A = Peritubular Dentine (around dentinal tubules)
B = Intertubular Dentine (btwn neighboring dentinal tubules)
Arrow head = Dentinal tubules

Mineralized
collagenous
matrix
The previous slides represent dentinal tubules that are
impeded in mineralized collagenous matrix

Vital dentinal tubules contain odontoblastic processes and

dentinal fluid

Unvital dentinal tubules are called dead tracts (coz

odontoblastic processes inside them become dead)

Here dentinal tubules appear as real tubules coz this is a

longitudinal section across dentine
In the previous slide dentinal tubules appear as circles coz
this is a cross section across dentine

Notice here odontoblastic processes inside dentinal tubules

(dark pink color)
In the previous slide notice the amount of branching of
dentinal tubules and odontoblastic processes inside them

Which layer of dentine is this??

Mantle dentine (1st layer of dentine in the crown)

How did I know??

the presence of a jxn which is scalloped in pattern and it is

the one that separate btwn enamel and dentine that's why
this layer of dentine is in the crown and because it has too
many branching it is mantle dentine

Notice also that in mantle dentine collagen fibers are parallel

to EDJ

B
A

A = Circumpulpal dentine in the crown

B = Circumpulpal dentine in the root

In both (crown and root) circumpulpal dentine is running

circumpulpally (around the pulp)
Arrow head = Predentine

B
A = Shreger line

B = contour lines of Owen

C = long period incremental lines of dentine (don't think that

these are dead tracts coz as you notice they are of unequal
length and don't extend through the entire thickness of
dentine)

This slide shows the dead tracts, notice how they are of
equal length and of approximate distances from each other
and how they extend through the entire thickness of dentine
and they are dead coz odontoblastic processes were killed
by a stimulus
 1

3/

3/

This is a slide showing different layers of dental pulp

1
Supraodontoblastic layer
2
Odontoblastic layer
3
Subodontoblastic layer
a. Cell free zone of Weil
b. Cell rich zone
4
Core of dental pulp
 1

This is a clearer slide showing dentine and dental pulp

1
Odontoblastic layer
2
Cell free zone of Weil
3
Cell rich zone
4
Predentine

4 3 2 1
This slide represents unerupting tooth

1
Reduced enamel epithelium (exists on the top surface of

enamel as long as the tooth isn't erupting yet but once it
erupts into the mouth it disappears and appears to cover the
cervical part of enamel only but not the surface)
2
Enamel (mature, appears as space)
3
Dentine
4
Dental pulp

1 2 3

This slide represents the apical part of the root

1
Root diaphragm (the only region at which the epithelial

root sheath cells don't disintegrate)
2
Proliferation zone of Dental papilla
3
Proliferation zone of Dental follicle
1

1
Epithelial rests of malassayz (remnants of ERS)

2
Root dentine
3
Odontoblasts
 Arrow heads = Epithelial rests of malassayz (aggregation of
cells in the PDL)

1st root 2nd root

** How many roots is this tooth going to have??

2 roots

5
3 4 6 7 8 9
1
Dentine
2
Predentine
3
Cementum
4
Cementoblasts
5
Epithelial rests of malassayz
6
PDL
7
Blood vessels
8
is it a blood vessel??
Of course not it is an osteon (building unit of bone)
9
Alveolar bone

Arrow head = Cellular cementum

2 Arrow heads = cementocytes

Arrow heads = Incremental lines of cementum
The encircled structure shows odontoclasts than are eating
the root of the deciduous tooth

Thank you all and good luck in your final exams

Done by:

Tahany M. Abu Al - Teen