Carbohydrate Polymers
journal homepage: www.elsevier.com/locate/carbpol
a r t i c l e i n f o a b s t r a c t
Article history: Diethylenetriamine-bacterial cellulose (EABC) was synthesized by amination with diethylenetriamine on
Received 26 May 2008 bacterial cellulose (BC). Its adsorption properties for Cu(II) and Pb(II) were investigated. The parameters
Received in revised form 30 June 2008 affecting the metal ions adsorption, such as contact time, solution pH, and initial metal ions concentra-
Accepted 1 July 2008
tion have been investigated. The adsorption kinetics and adsorption isotherms were further studied. The
Available online 9 July 2008
results show that the adsorption rate could be well fitted by pseudo-second-order rate model, and
adsorption isotherm could be described by the Langmuir model. The regeneration of EABC was also stud-
Keywords:
ied. This study provides the relatively comprehensive data for the EABC application to the removal of
Adsorption
Regeneration
metal ion in the wastewater.
Cu(II) Crown Copyright Ó 2008 Published by Elsevier Ltd. All rights reserved.
Pb(II)
Diethylenetriamine-bacterial cellulose
1. Introduction 2008), carboxy (Li, Yang, Zhao, & Xu, 2007), and carboxymethyl
(Pushpamalar, Langford, Ahmad, & Lim, 2006).
It is very important to remove heavy metals from wastewater As biosynthesized cellulose, Bacterial cellulose (BC) is an extra-
before it is drained because of the toxic effect to the health of hu- cellular cellulose produced by bacteria of genus Acetobacter
man being and other living beings. Regarding removal of metal (Klemm, Schumann, Udhardt, & Marsch, 2001). The primary struc-
ions from wastewaters, different technologies and processes are ture of BC is similar to that of plant cellulose: an unbranched poly-
currently used, such as: biological treatments, membrane process, mer of b-1, 4-linked glucopyranose residues. However, the fibrous
advanced oxidation processes, chemical and electrochemical tech- structure of BC is different from that of plant cellulose. BC is com-
niques, and adsorption procedures (Chauhan, Chauhan, Chauhan, posed of microfibrils, which have a ribbon-like structure, and the
Kumar, & Kumari, 2007; Vijayaraghavan, Palanivelu, & Velan, thickness (0.1 106 m) is two orders of magnitude smaller than
2006). Among these treatments, adsorption is considered to be that of plant cellulose (10 106 m). In addition, the microfibrils
an effective and economic method which has attracted a consider- retain a fine network structure. Because of its characteristic micro-
able interest (Celis, Hermosin, & Cornejo, 2000; Chern & Chien, structure, BC has various advantageous properties, such as high
2003; Oliveira, Franca, Oliveira, & Rocha, 2008). Lignocellulosic tensile strength, elasticity, large surface area, etc. Benefiting from
materials and wastes such as peanut skin, cotton, onion skin, rice its unique properties, BC has attracted an increased interest in
hulls, maize stalks, barks, jute fibers, bagasse, rice straw, corncobs, commercial applications over the past few years. Important exam-
and palm kernel husks have received much attention in heavy me- ples include raw materials for conductive carbon film, products for
tal ion removal (Axtell, Sternberg, & Claussen, 2003; Conrad & Han- temporary skin and tissue replacement, separation membrane, cal-
sen, 2007). Various functional groups such as carboxylates, orie-free food such as Coco de Nata, and additives in the produc-
phenolic and aliphatic hydroxyls, and carbonyl groups in these tion of lattices and paper (Czaja, Young, Kawecki, & Brown, 2007;
materials have the ability to adsorb some metal ions. To increase Iguchi, Yamanaka, & Budhiono, 2000; Klemm, Heublein, Fink, &
the metal ion adsorption of cellulose, it was chemically modified Bohn, 2005; Pandey, Saxena, & Dubey, 2005; Shah & Brown,
by introducing different complexing groups such as aminoalkyl 2005; Yano et al., 2005).
(Chauhan, Jaswal, & Verma, 2006), 2,2-diaminoethyl, and amidox- BC applied to adsorb metal ions has been reported in the previ-
ime (Saliba, Gauthier, Gauthier, & Petit-Ramel, 2000), or an ionic ous literature (Evans, O’Neill, Malyvanh, Lee, & Woodward, 2003),
moiety such as phosphate (Oshima, Kondo, Ohto, Inoue, & Baba, but the adsorption capacity is relatively low from the previous re-
2008), thiolate (Navarro, Bierbrauer, Mijangos, Goiti, & Reinecke, ports (Chen et al., 2008; Oshima et al., 2008). Therefore, it is neces-
sary to modify BC with a better mode in order to increase the
adsorption capacity. In this paper, we focused on BC as a starting
* Corresponding author. Tel.: +86 21 67792958; fax: +86 21 67792726.
material to prepare a new adsorbent, diethylenetriamine-bacterial
E-mail address: wanghp@dhu.edu.cn (H. Wang). cellulose (EABC), and studied its adsorption properties of Cu(II) and
0144-8617/$ - see front matter Crown Copyright Ó 2008 Published by Elsevier Ltd. All rights reserved.
doi:10.1016/j.carbpol.2008.07.006
W. Shen et al. / Carbohydrate Polymers 75 (2009) 110–114 111
Pb(II) at different conditions. The effects of the parameters such as bent at adsorption equilibrium was calculated according to the
pH, and contact time on the adsorption properties were investi- following Eq. (1):
gated. The adsorption kinetics of the first- and second-order kinet- qe ¼ ðC 0 C e ÞV=W ð1Þ
ics and Langmuir and Freundlich isotherms were used to evaluate
the adsorption process. The effect of regeneration of EABC using where C0 and Ce are the initial and equilibrium metal ions concen-
ethylenediaminetetraacetic acid (EDTA) on its adsorption capacity trations (mg/L), respectively, V is the volume of the solution (L) and
was also studied. W is the weight of the EABC used (g).
The treatment of BC in 15% alkaline solution called merceriza- 3.1. Preparation and characterization of EABC
tion was carried out for 1 h at a room temperature, followed by
extensive washing with water. Then the mercerized BC (1 g) was Hydroxyl groups of BC reacted with epichlorohydrin under
put in a round-bottom flask equipped with a magnetic stirring alkaline condition to give an epoxy derivative [Eq. (2)]. EABC was
bar. After this, a mixture of epichlorohydrin (3 mL) and 8% alkaline synthesized by treatment of the epoxy derivative with diethylene-
solution (100 mL) was added into the flask. The reaction was car- triamine [Eq. (3)].
ried out at 20 °C for 10 h under stirring. After reaction, the product O
O ð2Þ
was washed with deionized water and anhydrous ethanol by turns NaOH H2C CHCH2 Cl
Cell-OH Cell-ONa Cell-O-CH2 HC CH2
until pH 7.0 was reached, and finally dried at 60 °C. The treated BC
(1 g) was immersed in a mixture of diethylenetriamine (1 g) and OH
O NH 2(CH2)2NH(CH2)2NH2
deionized water (100 mL), and a desired amount of sodium bicar- Cell-O-CH2 HC CH2 Cell-O-CH2CHCH2NH(CH 2) 2NH(CH 2) 2NH2
bonate as a catalyst (10 g/L) was added under continuous stirring ð3Þ
at 50 °C for 2 h. After reaction, the product was washed with deion- Previous studies showed that the transformation of the epoxy
ized water and anhydrous ethanol by turns until pH 7, and dried at group to the amine group increased with the increase of amine
60 °C. Finally, white cellulosic ether powder was obtained. concentration and reaction temperature. Also, this transformation
increased sharply with time, and then tended toward a steady va-
2.3. Fourier transform-infrared (FT-IR) spectroscopy lue after 2 h (Gregorio, 2005). In this study, an optimum condition
previously reported by Hassan, El-Wakil, and Sefain (2001) was
The powder of BC, EABC, EABC with 43.19 mg/g Cu(II) adsorbed used to synthesize EABC. Nitrogen content in it is 13.12% by ele-
and EABC with 57.20 mg/g Pb(II) adsorbed were dried at 50 °C un- mental analysis. Under similar reaction condition, a nitrogen con-
der vacuum. Subsequently, 1 mg of the samples were mixed with tent of diethylenetriamine plant cellulose is only 4.12%. The
100 mg of spectroscopy grade KBr, dried at 50 °C under vacuum, results suggested that the amination (%) can be influenced by the
and pressed into a small tablet. FT-IR spectra were recorded on a morphology of cellulose. As BC microfibrils are much thinner than
NEXUS-670 Nicolet FT-IR instrument equipped with a MCT detec- fibers of plant cellulose, much more reactive hydroxyl groups on
tor in the absorption mode with a resolution of 4 cm1, in the range the surface of BC can be functionalized.
of 4000–400 cm1.
3.2. FT-IR spectroscopy
2.4. Elemental analysis
Fig. 1 shows the FT-IR spectra of BC, EABC, EABC with Cu(II) ad-
The amount of diethylenetriamine grafted on BC was calculated sorbed and EABC with Pb(II) adsorbed. The major peak located at
based on the nitrogen content, determined by an elemental ana- around 3400 cm1 in BC is attributed to –OH stretching vibration.
lyzer (Germany Elemental Analysensysteme GmbH, Vario EL III). But in EABC, the peak becomes broad because of the existence of –
NH2. The characteristic band at around 1060 cm1 corresponding
2.5. Adsorption experiments to C–O–C stretching is also observed in all the samples. Besides,
compared with the spectrum of BC, the increase of broad peak
EABC (0.01 g) was added to 10 mL of the different buffered me- around 1430 cm1 is observed which confirms the existence of –
tal ions solutions. The pH was adjusted using 0.1 N HNO3 or 0.1 N NH in EABC, but it weakens after Cu(II) and Pb(II) adsorption,
NaOH. The mixture was stirred for different times (10–180 min) at and the peak at around 3400 cm1 becomes broad and shifts to
room temperature (25 °C). The amount of remaining metal ions around 3420 cm1 after Cu(II) and Pb(II) adsorption. This suggests
was determined by an atomic absorption spectrophotometer (ZEE- the formation of the complex between metal ions and N atoms
nit 700, German). The amount of metal ions adsorbed on the adsor- (Nakano, Takeshita, & Tsutsumi, 2001).
112 W. Shen et al. / Carbohydrate Polymers 75 (2009) 110–114
30
a
Transmittance(%)
25
b
1430
qe(mg/g)
3400 20 2+
c Cu
2+
3400 Pb
d 15
3420
10
3429 1060
35 60
2+ 2+
Cu Cu
30 2+
50 Pb
2+
Pb
25
40
20
qe(mg/g)
qe (mg/g)
15 30
10
20
5
10
0
-5 0
2 3 4 5 6 7 0 200 400 600 800 1000 1200
pH C0(mg/L)
Fig. 2. The effect of pH on the amount of Cu(II), Pb(II) adsorbed onto EABC. Initial Fig. 4. The effect of metal ion concentration on EABC adsorption. pH 4.5 ± 0.1,
metal ion concentration 100 mg/L, pH 2 ± 0.1–5 ± 0.1, amount of EABC 0.01 g. amount of EABC 0.01 g, contact time 2 h.
W. Shen et al. / Carbohydrate Polymers 75 (2009) 110–114 113
integration by applying the initial conditions qt = 0 at t = 0 and Metal ion Langmuir parameter Freundlich parameter
qt = qt at t = t, Eq. (4) becomes b (L/mg) qm (mg/g) R2
KF (L/g) n R2
ln ð1 qt =qe Þ ¼ k1 t ð5Þ Cu(II) 0.002 63.09 0.9962 0.8028 1.68 0.9871
Pb(II) 0.003 87.41 0.9926 0.9952 1.61 0.9824
In addition, a pseudo-second-order equation based on adsorption
equilibrium capacity may be expressed in the form
dqt =dt ¼ k2 ðqe qt Þ2 ð6Þ with a slope of (1/qm) and intercept of 1/bqm and the calculated data
are listed in Table 2. It is found that the maximum adsorption
where k2 is the rate constant of pseudo-second-order adsorption.
capacity of Cu(II) and Pb(II) are 63.09 and 87.41 mg/g, respectively.
Integrating Eq. (6) and applying the initial conditions, we have
The bond energy (b) of Cu(II) and Pb(II) are 0.002 and 0.003 L/mg,
t=qt ¼ 1=k2 q2e þ t=qe ð7Þ respectively. It can be concluded that EABC presents greater adsorp-
tion capacity and larger bond energy for Pb(II) than that for Cu(II).
The values of qe, k1, and k2 can be calculated from the slopes and
The Langmuir parameters given in Table 2 can be used to pre-
the intercepts of the straight lines and the data are given in Table 1.
dict the affinity between the sorbate and adsorbent using the
As shown in the data, the correlation coefficients for the first-order
dimensionless separation factor RL (Bhatnagar & Jain, 2005):
kinetic model are lower than those of the second-order kinetic
model. Therefore, the Cu(II) and Pb(II) adsorption onto the EABC RL ¼ 1=ð1 þ bC 0 Þ ð11Þ
belong to the second-order kinetic model and the adsorption is a
The values of RL for adsorption of Cu(II) and Pb(II) onto EABC are
chemical process.
shown in Fig. 5. They indicate that adsorption of Cu(II) and Pb(II) is
more favorable at higher initial metal ion concentrations than at
3.7. Adsorption isotherms
lower ones. Based on the coefficient obtained, it can be concluded
that the Langmuir equation gives a better fit to the experimental
The adsorption data have been subjected to different adsorption
data than the Freundlich equation.
isotherms, namely the Freundlich and Langmuir.
3.8. Regeneration of EABC
3.7.1. Freundlich isotherm
The Freundlich model stipulates that the ratio of solute ad-
For potential practical application, it is important to examine
sorbed to the solute concentration is a function of the solution.
the possibility of desorbing the metal ions adsorbed on EABC and
The empirical model was shown to be consistent with exponential
reusing them (Liu & Bai, 2006). Both EDTA and HCl solutions were
distribution of active centers, characteristic of heterogeneous sur-
examined in the study for EABC. The effect of five adsorption–
faces. The amount of Cu(II) and Pb(II) adsorbed onto EABC at equi-
desorption consecutive cycles on the efficiency of the individual
librium, qe, is related to the concentration of Cu(II) and Pb(II) in the
adsorption of Cu(II) and Pb(II) on EABC was studied. Table 3 shows
solution, Ce, following (Deng, Bai, & Chen, 2003; Karagoz, Tay, Ucar,
the corresponding desorption efficiencies obtained at EDTA and
& Erdem, 2008):
HCl solutions. For desorption conducted with EDTA solution, it
qe ¼ K F C 1=n ð8Þ was found that the Cu(II) and Pb(II) adsorbed on EABC were easily
e
desorbed. The desorption efficiency reached about 99% after the
This expression can be linearized to give first cycle. The desorbed EABC was highly effective for the read-
ln qe ¼ ln K F þ ð1=nÞ ln C e ð9Þ sorption of Cu(II) and Pb(II), and the adsorption ability of EABC
was kept constant after several repetitions of the adsorption–
where KF and n are the Freundlich constants, which represent desorption cycles. For desorption conducted with the HCl solution,
adsorption capacity and adsorption intensity, respectively. A plot the desorption efficiency reached about 90% after the first cycle
of lnqe versus lnCe would result in a straight line with a slope of which is lower than the EDTA solution. And the adsorption capac-
(1/n) and intercept of lnKF and the constants are given in Table 2. ity reduced significantly after the third cycle. This may be attrib-
where qm and b, the Langmuir constants, are the saturated mono- 0.6
RL
0.4
Table 1
Pseudo-first and pseudo-second-order constants and values of R2 for the adsorption of
0.3
Cu(II) and Pb(II) onto EABC