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# Preparation of Buffers Claire Anne G.

## Punsalan De La Salle University-Dasmarias Dasmarias, Cavite

ABSTRACT In this experiment, the pH of different solutions and water were measured to see how pH was affected when acid or base was added into the solution. A buffer is a mixture of a weak acid [HA] and its conjugate base [A-] or a weak base and its conjugate acid. It is a solution that resists a drastic change in pH when acids or bases are added to it. Phosphate buffer (pH 7.40) was the buffer system used in the experiment. It was prepared using H2PO4--HPO4-2. A pH of 7.29 was prepared and tested. Adding 0.100 M HCl or 0.100 M NaOH to the solution tested the buffer. When 5 ml of 0.100 M HCl was added into the buffer, the pH decreased by 0.14 (7.15). When 5 ml of 0.100 M NaOH was added into the other buffer, the pH increased by 0.25 (7.54). It only shows that the buffer was able to resist drastic change when an acid or base was added into the solution. Unlike the buffer solution, the pH of distilled water was greatly affected when acid or base was added into it.

INTRODUCTION The objective if the experiment was to prepare a buffer system starting from needed calculations up to the actual preparation. Buffers are used in biochemistry to stabilize and modulate the pH of a solution. Creating a mixture of a weak acid and its conjugate base can make a buffer solution in a variety of ways. Buffer solutions are used to make a solution exhibit very little change in its pH when an acid or bases are added to it. Buffer solutions also have many applications in chemical manufacturing for processes that require a specific pH range to work. (1). The pH of a buffer solution is calculated by using Henderson-Hasselbalch equation. The equation has the formula pH= pKa + log [A-]/[HA]. The pH of a solution is equal to acid dissociation constant of the weak acid plus the logarithm of the ratio of conjugate base over weak acid. In biological systems, the buffer system of carbonic acid and sodium bicarbonate used to maintain the pH of blood to a constant value (2). In this experiment, buffer system was used to demonstrate how buffer resists drastic change when an acid or base was added.