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Introduction

The Companies of Harvard Bioscience.............ii Global Distribution.............................................iv New Products.....................................................vi Hoefer Product Range....................................viii

Power Supplies
EV 265 Consort.............................................37 Hoefer PS 2A200..............................................38

CONTENTS

Electrophoresis Reagents Protein Electrophoresis


Standard Vertical Systems...........................1-2 SE 600 Chroma................................................3 SE 600 and SE 660.........................................4 SE 640 Wide Mini............................................5 SE 400 and SE 410 Sturdier..........................6 Gel Casters for Standard Format Gels............7 Accessories & Spare Parts............................8-10 Gel Casting......................................................39 Sample Preparation and Loading....................40 Buffer and Buffer Preparation..........................41 Gel Staining......................................................42

Imaging
ULTima Imaging Platforms.....................43-48 ULTima 10si, 16si, 16si Plus and Pro........45-46 Optix Illumination Module.............................47 TotalLab Analysis Software.........................47-48 UV Transilluminators.....................................49

Protein Electrophoresis
Small Format Vertical Systems...............11-12 SE 300 miniVE.........................................13-14 Mighty Small II, SE 250 and SE 260.......15-16 Gel Casters for Small Format Gels..................17 Accessories.......................................................18

Quantitation
Fluorometer for DNA/RNA Quantitation.........50

Plate Washer & Storage Kit


SE 100.............................................................19

Detection
Detection..............................................51-54 HB-1000 Hybridization Oven......................51-52 UVC 5000 UV Crosslinker..........................51-52 SE 1200 Easy Breeze Gel Dryer....................53 GD 2000 Gel Dryer.........................................54

Gradient Makers
SG Series.......................................................20

Nucleic Acid Electrophoresis


Submarine Agarose Units.......................21-24 HE 33 Mini....................................................22 HE 99X Max...................................................23 Accessories & Spare Parts............................24

Radiation Safety Products


Beta and Gamma Radiation Protection......55-56

Protein Blotting
Blotting.................................................25-30 Semi-Dry Blotters...................................27-28 TE 70X and TE 70XP..................................27 TE 77X and TE 77XP..................................28 Membranes................................................34 Tank Blotters...........................................29-30 TE 22 Mighty Small....................................29 TE 62 and TE 42......................................30 Membranes................................................34

UV/ Visible Spectrophotometers


Biochrom Libra Series....................................57 Biochrom WPA Series....................................58

Amino Acid Analyzer


Biochrom 30 Series...................................59-60

Sample Preparation Immunoblotting


Immunoblotting...................................31-33 ImmunoBlot and ImmunoBlot XL................32 Slot Blot and DecaProbe..............................33 Membranes.................................................34 Harvard Apparatus Products......................61-62

General Information
Technical Appendix..........................................63 Online Ordering and Support........................64 Contact Information........................................65 Terms and Conditions.......................................66 Bibliography........................................67-68

Filtration
Filtration Manifold.........................................35

Custom Products
Custom Manufacturing.....................................69

Circulation Bath
Refrigerated & Heated Circulation Bath........36

HARVARD BIOSCIENCE

HARVARD BIOSCIENCE Family of Companies


Areas of Concentration
Molecular Biology Cell Biology Tissue Studies Isolated Organ Whole Animal
Pumps, Ventilators, Anesthesia and Sample Preparation Products Plus Thousands More. Physiology Recorders, Amplifiers, Transducers, Surgical Tables & Homeothermic Blankets The Technology Leader in Electroporation and Electrofusion. Equilibrium Dialysis, Dialysis, SPE and Sample Preparation Products Clark Electromedical World Standard Capillary Glass Hugo Sachs Elektronik Isolated Organ & Tissue Systems, Data Acquisition International Market Supply Anesthesia & Animal Behavior Products Micro-Injectors, Micro-Incubators, Brain Slice Chambers and Iontophoretic Pumps Diffusion/Ussing Chambers, Substance Transport Studies Patch Clamp Amplifiers & Systems, Micro-Perfusion Chambers, Cell Imaging Micro-Incubators, Ussing Systems

Company

Products

Biochrom Group

Harvard Apparatus Group

Spectrophotometers & Amino Acid Analyzers High Quality Electrophoresis, Blotting, Quantitation and Detection Systems and More. Microplate Readers, Washers & Dispensers Full Range of Electrophoresis & Radiation Protection Products Walden Precision Apparatus pH Meters, Conductivity Meters, Colorimeters, Oxygen Meters and Spectrophotometers

Genomic Solutions

Genomic & Proteomic Research Tools for Complete Drug Discovery Solutions Products for Large Bore Flow Cytometry Products for High Content Imaging & Analysis

ii

UBI Group

Contact Information
HARVARD BIOSCIENCE

United States
Harvard Apparatus 84 October Hill Road Holliston, Massachusetts 01746 Phone: 1-508-893-8999 Toll Free: 1-800-272-2775 (USA & Canada) Fax: 1-508-429-5732 Email: bioscience@harvardapparatus.com Web: harvardapparatus.com A wide variety of research products including industry leading syringe pumps, ventilators, microinjectors, microincubators, temperature controllers, capillary glass, advanced sample preparation products and many, many more. Hoefer, Inc. 953 Indiana Street San Francisco, California 94107 Phone: 1-415-282-2307 Toll Free: 1-800-227-4750 (USA & Canada) Fax: 1-415-920-4494 Email: sales@hoeferinc.com Web: hoeferinc.com High quality electrophoresis, blotting, quantitation and detection systems and more. Union Biometrica, Inc. US Headquarters 84 October Hill Road Holliston, Massachusetts 01746 Phone: 1-508-893-3115 Fax: 1-508-893-8044 Email: sales@unionbio.com Web: unionbio.com Products for large bore flow cytometry. Warner Instruments, LLC. 1125 Dixwell Avenue Hamden, Connecticut 06514 Phone: 1-203-776-0664 Toll Free: 1-800-599-4203 (USA & Canada) Fax: 1-203-776-1278 Email: sales@warneronline.com Web: warneronline.com The industry leader for electrophysiology and cell biology products including clamp amplifiers & systems, micro-perfusion and imaging chambers, Ussing systems and micro-incubators. Genomic Solutions, Inc. 4355 Varsity Drive, Suite E Ann Arbor, Michigan 48108 Phone: 1-734-975-4800 Toll Free: 1-877-436-6642 (USA & Canada) Fax: 1-734-975-4809 Email: info@genomicsolutions.com Web: genomicsolutions.com Genomic and proteomic research tools for complete drug discovery solutions.

Belgium
MAIA SCIENTIFIC Cipalstraat 3, B-2440 Geel, BELGIUM Phone: (32) 14-570-620 Fax: (32) 14-570-621 Email: sales@maia-scientific.com Web: maia-scientific.com Maia Scientific specializes in products for high content imaging & analysis.

United Kingdom
Harvard Apparatus, Ltd. Fircroft Way, Edenbridge Kent TN8 6HE, UNITED KINGDOM Phone: (44) 1732-864-001 Fax: (44) 1732-863-356 Email: sales@harvardapparatus.co.uk Web: harvardapparatus.co.uk Harvard Apparatus, Ltd. manufactures a wide range of physiology products including recorders, amplifiers, transducers, surgical equipment and the new IMS anesthesia product line. Biochrom, Ltd. Cambridge Science Park, Unit 22 Milton Road Cambridge CB4 0FJ, UNITED KINGDOM Phone: (44) 1223-423723 Fax: (44) 1223-420164 Email: enquiries@biochrom.co.uk Biochrom manufactures a full line of spectrophotometers and amino acid analyzers. Scie-Plas, Limited Unit 3 Gainsborough Trading Estate Old Road, Southam Warwickshire CV47 1HP, UNITED KINGDOM Phone: (44) 1926-814093 Fax: (44) 1926-813975 Email: Info@scie-plas.co.uk Scie-Plas manufactures high quality electrophoresis and radiation protection products. Walden Precision Apparatus, Ltd. Cambridge Science Park, Unit 22 Milton Road Cambridge CB4 0FJ, UNITED KINGDOM Phone: (44) 1223-423723 Fax: (44) 1223-420164 Email: sales@wpaltd.co.uk Walden Precision Apparatus brings a wide range of products to our offering including pH meters, conductivity meters, colorimeters, oxygen meters and spectrophotometers.

Canada
Harvard Apparatus Canada 6010 Vanden Abeele Saint Laurent, Quebec H4S 1R9, CANADA Phone: 1-514-335-0792 Toll Free: 1-800-361-1905 (Canada Only) Fax: 1-514-335-3482 Email: sales@harvardapparatus.ca Web: harvardapparatus.ca Harvard Apparatus Canada represents the same product offerings as Harvard Apparatus in the United States, as well as vibration tables and Biopac.

France
Harvard Apparatus S.A.R.L. 6 Avenue des Andes Miniparc - Bat. 8 91952 Les Ulis Cedex, FRANCE Phone: (33) 1-64-46-00-85 Fax: (33) 1-64-46-94-38 Email: info@harvardapparatus.fr Harvard Apparatus S.A.R.L. represents the same product offerings as Harvard Apparatus in the United States.

Germany
Hugo Sachs Elektronik Harvard Apparatus GmbH Gruenstrasse 1 March-Hugstetten D-79232, GERMANY Phone: (49) 0 7665-92-00-0 Fax: (49) 0 7665-92-00-90 Email: info@hugo-sachs.de Web: hugo-sachs.de Hugo Sachs Elektronik is the industry leader providing advanced isolated organ and tissue perfusion systems as well as the PLUGSYS modular amplifier system.

Austria
ASYS Hitech GmbH Nordstrasse 4 A 5301 Eugendorf, AUSTRIA Phone: (43) 6225-3241 Fax: (43) 6225-2549 Email: sales@asyshitech.com Web: asyshitech.com Asys Hitech specializes in microplate readers, washers and dispensers.

New Bioresearch Products Catalog


1100 page catalog now available. Call for your FREE copy:

800-272-2775
or +1-508-893-8999
iii

GLOBAL DISTRIBUTION

Global Distribution
China GeneTimes Technology Inc. Phone: + 86 2154 26 2677 www.genetimes.cn LabChina Company Limited Phone: + 86 20 38215851 www.labchina.cn Gene Company Ltd. Phone: + 85 2 896 6283 www.genehk.com

Hoefer is Back!

Canada Fisher Scientific Company Phone: + 1 800 234 7437 www.fishersci.ca

Japan LMS Co., Ltd. Phone: + 81 3 5842 4170 Korea: Seoulin Bioscience Phone: + 82 2 478 5911 www.seoulin.co.kr

United States Hoefer, Inc. Phone: + 1 800 227 4750 +1 415 550 3494 www.hoeferinc.com Fisher Scientific Phone: + 1 800 766 7000 www.fishersci.com

Taiwan: Unimed Healthcare Inc. Phone: + 886 2 27202215 www.unimed.com.tw Thailand: Becthai Bangkok ECC Ltd. Phone: + 66 2 615 2929 www.becthai.com Malaysia: Research Instruments Ltd. Phone: + 60 3 78 048600 www.research-instruments.com.sg Singapore: Research Instruments Ltd. Phone: + 65 6775 7284 www.research-instruments.com.sg Mexico DINELAB, S.A. DE C.V. Phone: + 52 55 5550 5065 www.dinelab.com

Hoefer is a company with a great tradition of serving life scientists and we are happy to work with them. Hoefer tillverkar utmrkta produkter som varar en livstid. Hoefer tiene gran productos.

Australia Bio-Strategy Phone: 1 800 00 84 53 www.bio-strategy.com

New Zealand Bio-Strategy Ltd. Phone: 0 800 DIAGNOSE (34 24 66) www.bio-strategy.com

La Qualit excellente et gamme la plus large. Wir sind stolz, Hoefer Produkte zu verkaufen. Il Hoefer ha di grandi prodotti per i nostri clienti.

iv

Global Distribution

For up to date distribution information see www.hoeferinc.com

Finland: Reactionlab Finland Oy Phone: + 358 2 410 1146 www.reaction-lab.com Sweden: Reaction Lab Sverige AB Phone: + 46 18 14 9002 Norway: Reactionlab AS Phone: + 46 18 14 9002 www.reaction-lab.com Russia The CIS and The Baltics Pribori OY Phone: + 358 9 502 12 55 www.egg.ru

GLOBAL DISTRIBUTION

Denmark: Reactionlab AS Phone: + 45 7027 9595 www.reaction-lab.com Netherlands: Isogen Life Science Phone: + 31 346 550556 www.isogen-lifescience.com

Germany Austria Fisher Scientific GmbH Phone: + 49 2304 932 5 www.de.fishersci.com UK Ireland Fisher Scientific UK Ltd Phone: + 44 (0) 1509 231166 www.fishersci.co.uk Germany NovoDirect GmbH Phone: + 49 (0) 7851 99457 0 www.novodirect.de Czech Republic Central European Biosystems s.r.o Phone + 420 603533444

France Belgium Spain Fisher Bioblock Scientific Phone: + 33 3 88 67 53 78 www.bioblock.com

Slovakia Central European Biosystems s.r.o Phone: + 421 905534365 Phone: + 42 0257 013 410

Portugal PACI Phone: + 351 21 4155742

Italy: Pirovano and Partners Phone: + 39 02 95 360 250 Analytical Control S.p.A. Phone: + 39 02 6129241 www.analyticalcontrol.it Switzerland Witec AG Phone + 41 412505357 www.witec.ch

Hungary Central European Biosystems KFT Phone: + 36 305279650

Israel Lumitron Electronic Instrument Phone: + 972 2652 9898 Turkey Commat Ltd. Phone: + 90 3124727417 India Blue Star Limited Phone: + 91 44 2815 8855 www.bluestar.com

Angola PACI Phone: + 351 21 4155740

Argentina Cientist S.A. Phone: + 54 11 48540804 www.scientist.com South Africa United Scientific (Pty) Ltd. Phone: + 27 21 5925240

Mozambique PACI Phone: + 351 21 4155740

Global Distribution

NEW PRODUCTS

New Products

from Hoefer

HOEFER SEMI-DRY TRANSFER UNITS use electrophoresis to transfer proteins and nucleic acids from polyacrylamide or agarose gels onto nitrocellulose or nylon membranes.

TE 70X SERIES SEMI-DRY TRANSFER UNITS

SEE PAGES 27-28

The HOEFER IMMUNOBLOT AND IMMUNOBLOT XL make it possible to assay the material on a transfer membrane with different antigens or antibody probes simultaneously without cutting the membrane into strips. The membrane remains intact to preserve the correspondence between different lanes or positions on the gel.

IMMUNOBLOT & IMMUNOBLOT XL BLOTTING MANIFOLDS

SEE PAGE 32

The HOEFER DQ 300 FLUOROMETER is a compact, dual-channel unit that delivers quick, easy, and accurate fluorescence measurements with either longwave UV or blue excitation.

DQ 300 FLUOROMETER DNA/RNA QUANTITATION

SEE PAGE 50

The HOEFER RCB-20 REFRIGERATED & HEATED CIRCULATION BATH provides efficient temperature control for all types of electrophoresis and transfer techniques.
RCB-20 REFRIGERATED & HEATED CIRCULATION BATH

SEE PAGE 36

vi

New Products

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

NEW PRODUCTS

CALIBER HIGH QUALITY ELECTROPHORESIS REAGENTS

SEE PAGES 39-42

HOEFER CALIBER ELECTROPHORESIS REAGENTS deliver consistently outstanding results. A high standard of purity, rigorous analytical procedures, and carefully controlled manufacturing techniques ensure that Hoefer Caliber reagents are the best quality buffer, gel casting, gel staining and sample preparation chemicals available.

The HOEFER UVC 5000 ULTRAVIOLET CROSSLINKER is excellent for cross-linking DNA or RNA to membranes for multiple probings of Southern, Northern, dot, or slot blots; for DNA nicking in agarose gels; and for sterilizing samples. The UVC 5000 can be stacked on top of the HB 1000 hybridizer to save valuable lab space.

The HB 1000 HYBRIDIZATION OVEN is designed to create the ideal environment for nucleic acid hybridization.

HB-1000 HYBRIDIZATION OVEN & UVC 5000 ULTRAVIOLET CROSSLINKER

SEE PAGES 51-52

The HOEFER ULTima is a conveniently upgradable imaging platform incorporating advanced electronics and optics. The four models of gel documentation systems can handle the most demanding applications in Proteomics and Genomics without expensive laser scanning systems.

ULTima IMAGING PLATFORM

SEE PAGES 43-48 New Products

vii

HOEFER PRODUCT RANGE

Hoefer Product Range


Complete Solutions for Electrophoresis Quantitation
DQ 300 FLUOROMETER DNA/RNA QUANTITATION

SEE PAGE 50

AIR COOLED VERTICAL ELECTROPHORESIS UNITS

SEE PAGE 6

Electrophoresis

miniVE SMALL FORMAT INTEGRATED ELECTROPHORESIS and BLOTTING UNIT

SEE PAGES 13-14

GRADIENT MAKERS

SEE PAGE 20

CALIBER HIGH QUALITY REAGENTS

HOEFER MIGHTY SMALL II MINI VERTICAL UNITS

SEE PAGES 39-42

SEE PAGES 15-16

DUAL COOLED VERTICAL ELECTROPHORESIS UNITS MINI SUBMARINE ELECTROPHORESIS UNITS

SEE PAGES 3-4

SEE PAGES 21-24

RCB-20 REFRIGERATED & HEATED CIRCULATION BATH

SEE PAGE 36
EV 265 CONSORT POWER SUPPLY

viii

Hoefer Product Range

SEE PAGE 37

SE 100 PLATE MATE GLASS PLATE WASHER & STORAGE KIT

SEE PAGE 19

Imaging & Documentation


HOEFER PRODUCT RANGE
GEL DRYERS

SEE PAGES 53-54

ULTima IMAGING PLATFORMS

SEE PAGES 43 - 48

Detection

MACROVUE UV TRANSILLUMINATORS

SEE PAGE 49

HB-1000 HYBRIDIZATION OVEN & UVC 5000 ULTRAVIOLET CROSSLINKER

Blotting
TE 70X SERIES SEMI-DRY TRANSFER UNITS

SEE PAGES 51-52

SEE PAGES 27-28

IMMUNOBLOT BLOTTING MANIFOLDS

SEE PAGES 31-34

PS 2A200 POWER SUPPLY

SEE PAGE 38

TRANSFER TANKS

SEE PAGES 29-30

Hoefer Product Range

ix

PROTEIN ELECTROPHORESIS
1
Vertical Electrophoresis

Hoefer pioneered the vertical format for electrophoresis separations and continues to offer innovative designs and a variety of choices.

PROTEIN ELECTROPHORESIS

Protein Electrophoresis
Dual Cooled & Air-Cooled Vertical Electrophoresis
Hoefer SE 600 SERIES DUAL COOLED TANK-STYLE VERTICAL UNITS run two or four gels simultaneously at the same temperature and a maximum of 112 samples when using divider plates. A heat exchanger suspended in the buffer tank cools buffer evenly, producing excellent separations with straight, sharp, well-defined bands.

SE 600X CHROMA DUAL COOLED VERTICAL ELECTROPHORESIS UNIT

SEE PAGE 3

SE 600 DUAL COOLED VERTICAL ELECTROPHORESIS UNIT

SEE PAGE 4

SE 400 SERIES STURDIER VERTICAL UNITS are air-cooled, affordably priced and run one gel (or two, by using a divider plate). SE 400 Series units are the ideal choice for labs requiring many units or applications where temperature control is not crucial.

SE 660 DUAL COOLED VERTICAL ELECTROPHORESIS UNIT

SEE PAGE 4

Separate, simple-to-use GEL CASTERS allow you to cast multiple identical gels at one time.

SE 400 STURDIER AIR COOLED VERTICAL ELECTROPHORESIS UNIT

SEE PAGE 6

CASTERS FOR VERTICAL ELECTROPHORESIS

A wide array of well-forming COMBS and SPACERS allows you to tailor gel configurations to your needs.
COMBS & SPACERS

SEE PAGE 7

SEE PAGE 10

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Vertical Electrophoresis

PROTEIN ELECTROPHORESIS

The Hoefer SE 600X, SE 600 and SE 660 are the high performance vertical units of choice in thousands of laboratories worldwide.

SE 600X CHROMA
Deluxe Dual Cooled Vertical Unit
The striking SE 600X CHROMA is the latest addition to the Hoefer SE 600 Vertical Series. Rounded contours allow for ease in handling, lifting, introducing sample, and disassembling. Produces straight lanes and sharp, well- defined bands. Runs as many as four gels (maximum 112 samples) at one time under identical conditions. Accepts gels 8-cm or 16-cm long. Accommodates denaturing and native polyacrylamide gels, and the second dimension of 2-D electrophoresis. A wide array of accessories enables you to tailor gel configurations to your needs. Runs gels at temperatures from 1 to 45 C.

ORDERING INFORMATION

Max. Glass Plate Size

18 x 16 cm

1-4 GELS

SE 600X CHROMA Item# SE600X-15-1.5 Includes: Upper Buffer Chamber Chroma Safety Lid w/ High Voltage Leads Chroma Lower Buffer Chamber Heat Exchanger Glass Plates 18 x 16 cm - 3 matched pairs Clamp Assemblies, 16 cm - 4 pcs Cams - 6 pcs Slotted Gaskets for UBC - 2 pcs Dual Gel Casting Stand with Leveling Base Spirit Level Laminated Gaskets for Caster - 2 pcs Buffer Dam Spacer-Mate - Alignment Template Wonder Wedge - Plate Separation Tool Gel Seal Combs, 15-Well - 2 pcs Spacers, 1.5-mm thick (2-cm wide) - 4 pcs

T ECHNICAL SPECIFICATIONS

Glass Plate Size 18 x 16 cm or 18 x 8 cm Spacers 0.75 mm, 1.0 mm, 1.5 mm Maximum Power Settings 1000 V, 500 mA, 50 W Operating Conditions Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): 32 x 29 x 14 cm (12.5" x 11.5" x 5.5") Safety Certifications EN61010-1 UL3101-1 CSA C22.2 1010.1 CE

Vertical Electrophoresis

SE 600 & 660


SE 600 accepts gels 8-cm and 16-cm long. SE 660, taller, but essentially the same design as the SE 600, accepts gels 8-, 16- and 24-cm long. Produce straight lanes and sharp, well-defined bands. Run up to four gels (maximum 112 samples) at one time under identical conditions.

PROTEIN ELECTROPHORESIS

Standard Dual Cooled Vertical Units

SE 660 accommodates 24 cm gels for increased resolution. Accommodate denaturing and native polyacrylamide gels, and 2-D electrophoresis. A wide array of accessories allows you to tailor gel configurations to your needs.
SE600 ORDERING INFORMATION SE660 ORDERING INFORMATION

Max. Glass Plate Size

T ECHNICAL SPECIFICATIONS

18 x 16 cm

1-4 GELS
Max. Glass Plate Size

Glass Plate Size SE 600: 18 x 16 cm or 18 x 8 cm SE 660: 18 x 24 cm, 18 x 16 cm or 18 x 8 cm Spacers: 0.75 mm, 1.0 mm, 1.5 mm Maximum Power Settings: 1000 V, 500 mA, 50 W Operating Conditions: Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): SE 600: 32 x 29 x 14 cm (12.5" x 11.5" x 5.5") SE 660: 32 x 37 x 14 cm (12.5" x 14.5" x 5.5") Safety Certifications EN61010-1, UL3101-1, CSA C22.2 1010.1 and CE

Item# SE600-15-1.5 Includes: Upper Buffer Chamber Safety Lid w/ High Voltage Leads SE 600 Lower Buffer Chamber Heat Exchanger Glass Plates 18 x 16 cm - 3 matched pairs Clamp Assemblies, 16 cm - 4 pcs Cams - 6 pcs Slotted Gaskets for UBC - 2 pcs Dual Gel Casting Stand with Leveling Base Spirit Level Laminated Gaskets for Caster - 2 pcs Buffer Dam Spacer-Mate - Alignment Template Wonder Wedge - Plate Separation Tool Gel Seal Combs, 15-Well - 2 pcs Spacers, 1.5-mm thick (2-cm wide) - 4 pcs Item# SE600 Includes: The same as above without the combs & spacers. Order two SE 511 Combs & two sets of 16-cm long Spacers separately, see page 10.

18 x 24 cm

1-4 GELS

Item# SE660 Order two SE 511 Combs & two sets of 24-cm long Spacers separately, see page 10. Includes: Upper Buffer Chamber Safety Lid w/ High Voltage Leads SE 660 Lower Buffer Chamber Heat Exchanger Glass Plates 18 x 24 cm - 3 matched pairs Clamp Assemblies, 8 cm - 4 pcs Clamp Assemblies, 16 cm - 4 pcs Cams - 6 pcs Slotted Gaskets for UBC - 2 pcs Dual Gel Casting Stand with Leveling Base Spirit Level Laminated Gaskets for Caster - 2 pcs Buffer Dam Spacer-Mate - Alignment Template Wonder Wedge - Plate Separation Tool Gel Seal

Clamp Spacers
DIVIDER PLATES DOUBLE GEL CAPACITY

Run as many as four 0.75-, 1.0- or 1.5-mm thick gels on SE 600 Series units and two gels on the SE 400 Series at one time under identical conditions. Divider Glass Plate (positioned between the outer plates) How it works: Fit a divider plate between the glass plates that make up a single sandwich. Put spacers on each side of the Outer Glass Plates divider plate to form two gel cavities for pouring gels. This two-gel club sandwich fits into the same casting stand and seals perfectly.
Vertical Electrophoresis

PROTEIN ELECTROPHORESIS

SE 640

Wide-Mini Electrophoresis Systems


The HOEFER SE 640 VERTICAL ELECTROPHORESIS UNIT is a wide-mini system, ideal for rapid screening of multiple samples. With the accessory divider plates you can complete a run of up to 112 samples in as little as 40 minutes! Permits rapid screening of up to 112 samples at one time. Design permits gels to be surrounded uniformly by buffer so heat dissipation is efficient. Bands are sharp and lanes are straight. Accommodates denaturing or native polyacrylamide gels. Run up to four 2-D gels using IEF strips or tube gels. Ideal for optimizing 2DE protocols.

ORDERING INFORMATION
Max. Glass Plate Size

T ECHNICAL SPECIFICATIONS

Glass Plate Size: 18 x 8 cm

18 x 8 cm
SE 640 Vertical Electrophoresis Unit, Complete Item# SE640-15-1.5 Includes: SE 640 Lower Buffer Chamber SE 640 Upper Buffer Chamber Glass Plates 18 x 8 cm 3 matched pairs 8 cm Clamp Assemblies 4 pcs Cams 6 pcs Dual Casting Stand w/Leveling Base Laminated Gaskets for Casting Stand 2 pcs Slotted Gaskets for UBC 2 pcs Buffer Dam Spacer-Mate alignment template Wonder Wedge plate separation tool Gel Seal Spirit Level SE 600/660 Lid 15-Well Combs 2 pcs 1.5-mm Thick Spacers (2-cm wide) 4 pcs

Spacers: 0.75 mm, 1.0 mm, 1.5 mm


1- 4 GELS

Maximum Power Settings: 1000 V, 500 mA and 50 W Operating Conditions: Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): 32 x 29 x 14 cm (12.5 x 11.5 x 5.5 in) Safety Certifications EN61010-1, UL3101-1, CSA C22.2 1010.1, CE

SE 640 Vertical Electrophoresis Unit, Basic Item# SE640 (without combs & spacers) Order SE 511 Comb and two sets of 8-cm long Spacers separately, see page 10. Includes: SE 640 Lower Buffer Chamber SE 640 Upper Buffer Chamber Glass Plates 18 x 8 cm 3 matched pairs 8 cm Clamp Assemblies 4 pcs Cams 6 pcs Dual Casting Stand w/Leveling Base Laminated Gaskets for Casting Stand 2 pcs Slotted Gaskets for UBC 2 pcs Buffer Dam Spacer-Mate alignment template Wonder Wedge plate separation tool Gel Seal Spirit Level SE 600/660 Lid

Accessories & Spare Parts


Most of the spare parts are shared with the larger SE 600 (see pages 8-10), EXCEPT for the following: UBC for SE 640, Item# SE6454 LBC for SE 640, Item# SE6450 Buffer Dam for SE 640, Item# SE6432

CRAFTSMANSHIP AT ITS BEST!

Hoefer has been fabricating scientific instruments for decades. You will never see bubbles or gaps in our glue seams. We use the correct environment, formula and technique to get perfect results every time. The result is products that perform and look the best -- thats our reputation.

Vertical Electrophoresis

SE 400 Series Sturdier vertical gel units live up to their name. Affordably priced, the SE 400 and SE 410 are ideal for laboratories requiring several units and for applications where temperature control is not crucial.
PROTEIN ELECTROPHORESIS

SE 400 Series
SE 400 runs one 16-cm long gel. SE 410, the taller version of the SE 400, runs one 24-cm long gel. The rugged injection-molded construction is durable and assembles easily. Run two gels with the addition of a divider plate. Built-in casting stand seals leak-free. Choose from a wide variety of accessories. Plates, clamps, spacers and combs are interchangeable with the SE 600.

Sturdier Air-Cooled Vertical Electrophoresis

SE400 ORDERING INFORMATION

T ECHNICAL SPECIFICATIONS

SE410 ORDERING INFORMATION

Max. Glass Plate Size

18 x 16 cm
Item# SE400-15-1.5

1-2 GELS

Glass Plate Size SE 400: 18 x 16 cm SE 410: 18 x 24 cm Spacers: 0.75 mm, 1.0 mm, 1.5 mm Maximum Power Settings: 500 V @ 40 mA, 30 mA per gel, 20 W Operating Conditions: Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): SE 400: 24 x 28 x 15 cm (9.5" x 11" x 6") SE 410: 24 x 36 x 15 cm (9.5" x 14.2" x 6") Safety Certifications EN61010-1, UL3101-1, CSA C22.2 1010.1 CE
Max. Glass Plate Size

18 x 24 cm

1-2 GELS

Includes: Upper Buffer Chamber Safety Lid with Electrodes Lower Buffer Chamber & Casting Stand on Leveling Base with High Voltage Leads Glass Plates, 18 x 16 cm - matched pair Clamp Assemblies, 16 cm - 2 pcs Cams - 2 pcs Slotted Gasket for UBC Spirit Level Laminated Gasket for Casting Stand Spacer-Mate - Alignment Template Wonder Wedge - Plate Separation Tool Gel Seal Comb, 15-Well Spacers, 1.5-mm thick (2-cm wide) - 2 pcs

Item# SE410 (without combs & spacers) Order SE 511 Comb and a set of 24-cm long Spacers separately, see page 10. Includes: Upper Buffer Chamber Safety Lid with Electrodes Lower Buffer Chamber & Casting Stand on Leveling Base with High Voltage Leads Glass Plates, 18 x 24 cm - matched pair Clamp Assemblies, 8 cm - 2 pcs Clamp Assemblies, 16 cm - 2 pcs Cams - 2 pcs Slotted Gasket for UBC Spirit Level Laminated Gasket for Casting Stand Spacer-Mate - Alignment Template Wonder Wedge - Plate Separation Tool Gel Seal

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Vertical Electrophoresis

Multiple Gel Casters allow you to cast several uniform or gradient gels at a time under identical conditions.
PROTEIN ELECTROPHORESIS

Gel Casters
Multiple Gel Casters SE 675 casts up to four 8- or 16-cm long gels. SE 615 casts up to ten 8- or 16-cm long gels. Easy to use and leak-free. Cast uniform or gradient gels. Pour uniform gels directly into the top of the casting chamber; pour gradient gels through an inlet port at the bottom.

For SE 600 & SE 400 Series Vertical Electrophoresis


Dual Gel Caster SE 6015 Dual Gel Caster is part of the package when you order an SE 600 Series unit. It can also be ordered separately. Casts two 8-, 16-, or 24-cm long gels at a time. Easy to use and leak-free. Glass sandwiches seal into the casting cradle with a simple twist of the cams.

SE615 ORDERING INFORMATION

SE675 ORDERING INFORMATION

SE6015 ORDERING INFORMATION

Max. Glass Plate Size

Max. Glass Plate Size

18 x 16 cm

up to 10 GELS

18 x 16 cm

up to 4 GELS

Max. Glass Plate Size

18 x 24 cm

1-2 GELS

SE 615 Multiple Gel Caster Item# SE615 Order SE 511 Combs and sets of Spacers separately, see page 10. Includes: Caster Body with Gasket Face Plate Clamps - 4 pcs Glass Plates, 18 x 16 cm - 10 matched pairs Filler Sheets - 5 pcs Wax Paper - 100 sheets, precut Spacer-Mate - Alignment Template Thumbscrews - 2 pcs Filler Plug Black Vinyl Cap Gel Seal Spirit Level

SE 675 Four Gel Caster Item# SE675 Order SE 511 Combs and sets of Spacers separately, see page 10. Includes: Caster Body with Gasket Face Plate Clamps - 4 pcs Glass Plates, 18 x 16 cm - 4 matched pairs Filler Sheets - 5 pcs Wax Paper - 100 sheets, precut Spacer-Mate - Alignment Template Thumbscrews - 2 pcs Filler Plug Set Black Vinyl Cap Gel Seal Spirit Level

SE 6015 Dual Gel Caster Item# SE6015 Order Glass Plates, SE 511 Combs and sets of Spacers separately, see page 10. Includes: Caster Stand with 2 Cradles & Leveling Feet Laminated Gaskets - 2 pcs

Silicone Filler Plug (for SE 615) Item# SE618


Vertical Electrophoresis

Silicone Filler Plug Set (for SE 675) Item# SE678

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Accessories & Spare Parts


SE 600 Vertical Electrophoresis
Lid for SE 600X Chroma Item# SE6056X

Laminated Gaskets - 2 pcs for Casting Stand Item# SE6009

PROTEIN ELECTROPHORESIS

Spirit Level Item# SER11 Casting Stand Includes: Caster Stand with 2 Cradles Leveling Feet Laminated Gaskets - 2 pcs Item# SE6015

Cams Black - 4 pcs Item# SE6005L

Upper Buffer Chamber (UBC) for SE 600 Series Item# SE6054 Slotted Gaskets - 2 pcs for UBC Item# SE6008B

Lid for SE 600/660 Item# SE6056

Buffer Dam Item# SE6032 Lower Buffer Chamber (LBC) for SE 600 Item# SE6150

Clamp Assembly 16 cm - 2 pcs Item# SE6003U Clamp & Cam Kit Includes: Clamp Assembly 16 cm - 4 pcs Cams Black - 8 pcs Item# SE6003UK (not pictured)

Glass Plates & Spacers See page 10 Clamp Assembly 8 cm - 2 pcs Item# SE6403U

Heat Exchanger for SE 600 Series Item# SE6160

Lower Buffer Chamber (LBC) for SE 660 Item# SE6650

Lower Buffer Chamber (LBC) for SE 600X Chroma Item# SE6150X


Vertical Electrophoresis

PROTEIN ELECTROPHORESIS

Accessories & Spare Parts


SE 410 Lid Item# SE416

GLASS PLATES
18 x 8 cm Regular Item# SE6402 (2 pcs) 18 x 8 cm Low Fluorescence Item# SE6402LF (2 pcs) 18 x 8 cm Divider Plate, Notched Item# SE6402D

SE 600 & SE 400 Vertical Electrophoresis

18 x 16 cm Regular Item# SE6102 (2 pcs) 18 x 16 cm Low Fluorescence Item# SE6102LF (2 pcs)

18 x 16 cm Divider Plate, Notched Item# SE6102D

18 x 24 cm Regular Item# SE6602 (2 pcs)

18 x 24 cm Low Fluorescence Item# SE6602LF (2 pcs)

SE 400 Lid Item# SE4156

18 x 24 cm Divider Plate, Notched Item# SE6602D

SE 400 UBC Item# SE4154

GEL SANDWICH COMPARISON

18 x 24 cm (WxH) Gel Sandwich Slotted Gasket (for SE 400 UBC) Item# SE4008B Laminated Gasket (for SE 400 LBC) Item# SE4009 18 x 16 cm (WxH) Gel Sandwich

SE 400 LBC Item# SE4151 18 x 8 cm (WxH) Gel Sandwich

Vertical Electrophoresis

COMBS

SPACERS

PROTEIN ELECTROPHORESIS

0.75-mm Thick

1.0-mm Thick

1.5-mm Thick Our combs are fabricated from plastics that are highly resistant to acids, bases, and all commonly used buffer systems. They are lightweight and virtually unbreakable when used with reasonable care.
All 1.0 mm Spacers are white for easy identification.

2 cm Width 1 cm Width

All spacers are molded from durable plastics for high chemical resistance and optimum tensile strength.

Thickness 0.75 mm 1.0 1.5 0.75 1.0 1.5 0.75 1.0 1.5 1.0 1.5

Length 8 cm 8 8 16 16 16 24 24 24 16 16

Width 2 cm 2 2 2 2 2 2 2 2 1 1

Qty. 2 2 2 2 2 2 2 2 2 2 2

Item# SE6419-2-.75 SE6419-2-1.0 SE6419-2-1.5 SE6119-2-.75 SE6119-2-1.0 SE6119-2-1.5 SE6619-2-.75 SE6619-2-1.0 SE6619-2-1.5 SE6118-2-1.0 SE6118-2-1.5

Gel Seal Item# SE6070

Standard Combs come attached to a standard back, which produces maximum well depths of 25 mm, except for the 28 well combs which produce well depths of 15 mm. # of Well Wells Thickness Width Qty. Item# 10 0.75 mm 8.3 mm 1 SE511-10-.75 1 SE511-10-1.0 8.3 10 1.0 1 SE511-10-1.5 8.3 10 1.5 1 SE511-12-.75 7.6 12 0.75 1 SE511-12-1.0 7.6 12 1.0 1 SE511-12-1.5 7.6 12 1.5 1 SE511-15-.75 5.7 15 0.75 1 SE511-15-1.0 5.7 15 1.0 1 SE511-15-1.5 5.7 15 1.5 1 SE511-20-.75 4.1 20 0.75 1 SE511-20-1.0 4.1 20 1.0 1 SE511-20-1.5 4.1 20 1.5 1 SE511-28-.75 2.7 28 0.75 1 SE511-28-1.0 2.7 28 1.0 1 SE511-28-1.5 2.7 28 1.5 1 SE511-BKA Adjustable Comb Back (Converts 25-mm deep comb to 10 or 15-mm depth)

Preparative Combs have adjustable comb backs (10-, 15- and 25-mm depth). # of Well Wells Thickness Width Qty. Item# 1/1 0.75 mm 121/6 mm 1 SE511-R-.75 1 SE511-R-1.0 121/6 1/1 1.0 1 SE511-R-1.5 121/6 1/1 1.5 1 SE511-DR-.75 113/6 1/2 0.75 1 SE511-DR-1.0 113/6 1/2 1.0 1 SE511-DR-1.5 113/6 1/2 1.5

Clamp Thumbscrews - 12 pk (for 8 or 16 cm Clamp) Item# SE6003U-2

High-Voltage Safety Lead Set Item# SE6056-HV

Wonder Wedge Plate Separation Tool Item# SE1514


Vertical Electrophoresis

10

PROTEIN ELECTROPHORESIS
11
Small Format Vertical Electrophoresis

Protein Electrophoresis
Hoefers small format vertical electrophoresis systems require reduced gel and buffer volumes and shorter separation times than the standard sized units. The SE 300 MINI VERTICAL UNIT allows you to cast, run, and blot two gels in 10 x 10.5 cm glass plates in one convenient instrument. Tank-format passive cooling assures the temperature uniformity required to achieve straight lanes and flat bands.

PROTEIN ELECTROPHORESIS

Small Format Vertical Electrophoresis

The SE 250 and SE 260 MIGHTY SMALL VERTICAL ELECTROPHORESIS UNITS run two mini gels at a time. SE 250 runs gels cast in 10 x 8 cm glass plates; the SE 260, in 10 x 10.5 cm glass plates. The small formats combined with efficient cooling allow rapid screening of proteins and nucleic acids.

SE 300 miniVE SMALL FORMAT INTEGRATED ELECTROPHORESIS and BLOTTING UNIT

SEE PAGE 13
SE 260 HOEFER MIGHTY SMALL II MINI VERTICAL UNIT

SEE PAGE 15

Separate, simple-to-use GEL CASTERS allow you to cast multiple gels at one time under uniform conditions.

SE 250 HOEFER MIGHTY SMALL II MINI VERTICAL UNIT

SEE PAGE 15

CASTERS FOR SMALL FORMAT VERTICAL ELECTROPHORESIS

SEE PAGE 17

Alumina Plates and Glass Plates


SEE PAGE 18

A wide array of well-forming COMBS AND SPACERS allows you to tailor gel configurations to your needs.
SEE PAGE 18 Small Format Vertical Electrophoresis

12

ORDERING INFORMATION

PROTEIN ELECTROPHORESIS

SE 300 miniVE
The HOEFER miniVE unit allows you to perform both electrophoresis and electrotransfer in one sturdy, compact unit. Cast, run, and blot two long mini gels in one instrument. Quick, excellent results use minimal buffer. Efficient cooling produces gels with straight lanes and flat bands. Cast your own or use pre-cast gels from a variety of manufacturers.

Max. Glass Plate Size

10 x 10.5 cm

1-2 1-2 GELS GELS

Small Format Integrated Electrophoresis and Blotting Unit

Transfer 4 gels in 45 minutes with only 300 ml buffer using optional SE 302 Blot Modules. Sturdy, safe, and easy to use.

SE 300 miniVE Item# SE300-10A-1.0 Includes: Lower Buffer Chamber (Tank) Gel Modules with Gaskets 2 assemblies Safety Lid with High Voltage Leads Glass Plates, Rectangular, 10 x 10.5 cm 3 pcs Glass Plates, Notched, 10 x 10.5 cm 3 pcs Combs, 10-well 2 pcs T-Spacers, 1.0-mm thick 4 pcs Well-Locating Decals 2 pcs Wonder Wedge Plate Separation Tool Gel Seal

SE 302 Blot Module Item# SE302 Includes: Blot Module Foam Sponges 6 mm thick - 3 pcs Blotter Paper - 25 sheets

T ECHNICAL SPECIFICATIONS

Glass Plate Size: 10 x 10.5 cm or 10 x 8 cm Spacers: 0.75 mm, 1.0 mm, 1.5 mm Maximum Power Settings for electrophoresis: 500 V and 25 W per module for blotting: 400 mA and 15 W per module Operating Conditions Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): 19.2 x 17.2 x 18.8 cm (7.6" x 6.8" x 7.4") Safety Certifications EN61010-1, UL3101-1, CSA C22.2 1010.1, CE

13

Small Format Vertical Electrophoresis

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Spare Parts
miniVE Lid w/high-voltage leads Item# SE306

PROTEIN ELECTROPHORESIS

miniVE

miniVE Banana Plug Kit (2 plugs, 2 nuts, 4 washers) Item# SE314

Gasket, 100 cm Item# FH2208

miniVE Clamp Screws (5 pcs) Item# SE311

miniVE UBC Assembly with Gasket & Banana Plug Kit Item# SE309 miniVE Clamp Assembly Item# SE310

miniVE Sealing Plate Assembly (includes Sealing Gasket) Item# SE312 miniVE Sealing Gasket Item# SE313

miniVE Tank (LBC) Item# SE308


Small Format Vertical Electrophoresis

14

SE 250 runs two gels cast in 10 x 8 cm glass plates.

PROTEIN ELECTROPHORESIS

Hoefer Mighty Small II

SE 260 runs two gels cast in 10 x 10.5 cm glass plates; 30% more resolving distance for complex samples. Run as many as 30 sample lanes. Efficient cooling ensures flat bands. Quick to assemble; easy and safe to use.

Small Format Vertical Electrophoresis

The Hoefer SE 250 combines the advantages of small format and efficient cooling for rapid screening of proteins and nucleic acids. The Hoefer SE 260 runs taller gels with the same efficient cooling and compact design.

Requires minimal buffer. Choose from a wide variety of accessories.

SE 250

SE 260

T ECHNICAL SPECIFICATIONS

SE250 ORDERING INFORMATION

SE260 ORDERING INFORMATION

Glass Plate Size SE 250: 10 x 8 cm SE 260: 10 x 10.5 cm or 10 x 8 cm Spacers: 0.75 mm, 1.0 mm, 1.5 mm Maximum Power Settings: 500 V, 500 mA, 12 W Operating Conditions: Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): SE 250: 16.5 x 16 x 16 cm (6.5" x 6.3" x 6.3") SE 260: 16.5 x 18 x 16 cm (6.5" x 7.1" x 6.3") Safety Certifications EN61010-1, UL3101-1, CSA C22.2 1010.1 and CE

Max. Glass Plate Size

10 x 8 cm

1-2 GELS

Max. Glass Plate Size

10 x 10.5 cm

1-2 GELS

Item# SE250-10A-.75 Includes: Lower Buffer Chamber Upper Buffer Chamber / Cooling Core Safety Lid with High Voltage Leads Casting Cradle w/Sealing Gasket Set Casting Clamp Assemblies 2 pcs Cams 4 pcs Glass Plates, Rectangular, 10 x 8 cm 10 pcs Alumina Plates, Notched, 10 x 8 cm 2 pcs Spring Clamps, Red 4 pcs Combs, 10-well 2 pcs T-Spacers, 0.75-mm thick 4 pcs Well-Locating Decals 2 pcs Gel Seal

Item# SE260-10A-.75 Includes: Lower Buffer Chamber Upper Buffer Chamber / Cooling Core Safety Lid with High Voltage Leads Casting Cradle w/Sealing Gasket Set Casting Clamp Assemblies 2 pcs Cams 4 pcs Glass Plates, Rectangular, 10 x 10.5 cm 10 pcs Alumina Plates, Notched, 10 x 10.5 cm 2 pcs Spring Clamps, Red 4 pcs Combs, 10-well 2 pcs T-Spacers, 0.75-mm thick 4 pcs Well-Locating Decals 2 pcs Gel Seal

15

Small Format Vertical Electrophoresis

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Spare Parts

High-Voltage Leads Item# SE6056-HV

PROTEIN ELECTROPHORESIS

Mighty Small II, Small Format Vertical Electrophoresis

SE 250 or SE 260 Lid with High-Voltage Leads Item# SE256

Decal, Well-Locating Item# SE212

QuickFit connectors, female 1/4" (2 pcs) Item# QF1/4

Spring Clamps, Red, Asymmetric (4 pcs) Item# SE252

QuickFit connectors, female 3/8" (2 pcs) Item# QF3/8 QuickFit connectors, male 3/8" (2 pcs) Not Pictured Item# QFX3/8

UBC/Cooling Core (includes Foam Gasket) Item# SE254B

Hardware Repair Kit - Not Pictured (2 banana plugs, 6 washers, 2 nuts, Teflon tubing 3" (3 pcs), 4 acetal rivets) Item# SE250RK-1

Foam Gasket, 61 cm Item# SE208 LBC for SE 260 Item# SE255D

LBC for SE 250 Item# SE255

Small Format Vertical Electrophoresis

16

Multiple Gel Casters allow you to cast several uniform or gradient gels at a time under identical conditions.
PROTEIN ELECTROPHORESIS

Gel Casters
Multiple Gel Casters HOEFERS SE 215 and SE 275 MULTIPLE GEL CASTERS allow you to cast several uniform or gradient gels in 10 x 8 cm plates under identical conditions. SE 275 casts from 1 to 4 gels simultaneously; SE 215 casts as many as 10 gels simultaneously. Pour uniform gels from the top; pump gradient gels into an inlet port at the bottom of the unit. Sandwiches fill at the same time at the same rate from the same solution. The result: identical gels. Glass plate sandwiches seal leak-free without the use of messy grease or tape. Once cast, gels can be wrapped in plastic and refrigerated for several weeks.

Small Format Vertical Electrophoresis


Dual Gel Caster Cast one or two gels with the SE 245 DUAL GEL CASTER. Accommodates both 10 x 8 cm and 10 x 10.5 cm plates. Uses the same rugged and effective clamp/cam casting method as used with the SE 400 and SE 600.

SE245 ORDERING INFORMATION

Max. Glass Plate Size

10 x 10.5 cm

1-2 GELS

SE 245 Dual Gel Caster Item# SE245 Includes: Casting Cradle Casting Clamp Assemblies 2 pcs Sealing Gasket Set Cams 4 pcs

SE275 ORDERING INFORMATION

SE215 ORDERING INFORMATION

Max. Glass Plate Size

10 x 8 cm
1-4 GELS

Max. Glass Plate Size

10 x 8 cm

1-10 GELS

Accessories
SE 215 and SE 275
Wax Paper Sheets (100 pcs), Item# SE201 Filler Sheets, Polycarbonate (5 pcs), Item# SE213 Space-saver Plate, Item# SE217 Face Plate, Item# SE218 Filler Plug Set for SE 215 (3 pcs), Item# SE209 Filler Plug Set for SE 275 (3 pcs), Item# SE279 Foam Gaskets, (61 cm), Item# SE208 Spring Clamps, Red, (4 pcs), Item# SE252 Red Inlet/Outlet Fitting, Item# XPO10

SE 275 Four Gel Caster Item# SE275 Combs & Spacers sold separately (see pages 18-19) Includes: Caster Body with Gasket Face Plate with Inlet Port Glass Plates, Rectangular, 10 x 8 cm 10 pcs Alumina Plates, Notched, 10 x 8 cm 4 pcs Spring Clamps, Red 2 pcs Wax Paper 100 sheets Space-Saver Plate Polycarbonate Filler Sheets 5 pcs Black Vinyl Cap Filler Plug Set

SE 215 Multiple Gel Caster Item# SE215 Combs & Spacers sold separately (see pages 18-19) Includes: Caster Body with Gasket Face Plate with Inlet Port Glass Plates, Rectangular, 10 x 8 cm 20 pcs Alumina Plates, Notched, 10 x 8 cm 10 pcs Spring Clamps, Red 2 pcs Wax Paper 100 sheets Space-Saver Plate Polycarbonate Filler Sheets 5 pcs Black Vinyl Cap Filler Plug Set

SE 245
Sealing Gasket Set, Item# SE246 Cams (4 pcs), Item# SE6005L Casting Clamp Assembly, Item# SE249 Clamp Thumbscrews (12 pcs), Item# SE6003U-2

17

Small Format Vertical Electrophoresis

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Accessories
COMBS
Combs: Standard # of Well Wells Thickness Width 5 0.75 mm 13.0 mm 13.0 5 1.0 13.0 5 1.5 5.8 9* 1.0 4.8 10 0.75 4.8 10 1.0 4.8 10 1.5 2.9 15 0.75 2.9 15 1.0 2.9 15 1.5 2.9 18* 1.0 *Microtiter Spacing

PROTEIN ELECTROPHORESIS

Small Format Vertical Electrophoresis


GLASS PLATES
10 x 8 cm (10 pcs) Rectangular Glass Plates Item# SE202P-10 10 x 8 cm (5 pcs) Notched Glass Plates Item# SE202GN-5 10 x 10.5 cm (5 pcs) Rectangular Glass Plates Item# SE262P-5 10 x 10.5 cm (5 pcs) Notched Glass Plates Item# SE262GN-5

ALUMINA PLATES
10 x 8 cm (1 pc) Notched Alumina Plates Item# SE202N 10 x 8 cm (10 pcs) Notched Alumina Plates Item# SE202N-10 10 x 10.5 cm (5 pcs) Notched Alumina Plates Item# SE262N-5

Well Volume (l per 1 mm depth) Qty. 1 9.8 1 13.0 1 19.5 1 5.8 1 3.6 1 4.8 1 7.2 1 2.2 1 2.9 1 4.4 1 2.9

Item# SE211A-5-.75 SE211A-5-1.0 SE211A-5-1.5 SE211A-9-1.0 SE211A-10-.75 SE211A-10-1.0 SE211A-10-1.5 SE211A-15-.75 SE211A-15-1.0 SE211A-15-1.5 SE211A-18-1.0

Combs: Preparative Preparative Combs form 1 large preparative well plus 1 small reference well. # of Well Well Volume Wells Thickness Width (l per 1 mm depth) Qty. Item# 1/1 0.75 mm 68/5 mm 51/3.8 1 SE211A-R-.75 68/5 68/5 1/1 1.0 1 SE211A-R-1.0 120/7.5 68/5 1/1 1.5 1 SE211A-R-1.5

T-SPACERS
All spacers are molded from durable plastics 0.75-mm for high chemical resistance and optimum Thick tensile strength. 2 cm Width Thickness Length Width Qty. Item# 0.75 mm 8 cm 1 cm 2 SE2119T-2-.75 1.0-mm Thick 2 SE2119T-2-1.0 1 1.0 1 cm 8 Width 1.5-mm 2 SE2119T-2-1.5 1 8 1.5 Thick 2 SE2619T-2-.75 10.5 1 0.75 All 1.0 mm 2 SE2619T-2-1.0 10.5 1 1.0 T-Spacers are white 2 SE2619T-2-1.5 10.5 1 1.5 for easy identification.

ONLY THE BEST!

Wonder Wedge Plate Separation Tool Item# SE1514

Spirit Level Item# SER11 Gel Seal 1/4 oz. Item# SE6070

At Hoefer we use only the best in materials to ensure performance, quality and longevity. The lower buffer chamber of a Mighty Small II unit is wired with platinum wire.

Small Format Vertical Electrophoresis

18

GLASS PLATE WASHER

SE 100 Plate Mate

Glass Plate Washer & Storage Kit

The SE 100 PLATE MATE WASHER AND STORAGE KIT dramatically reduces glass plate loss due to chipping and cracking. Glass plates fit securely into the plate holders grooves for safe washing, rinsing, and storage. Stainless steel bail handles let you carry plate holders easily and set them safely into the washing bath. Accommodates forty 8-cm tall or twenty 18-cm wide plates. Holds as many as twenty 10.5-cm tall plates with the optional SE 103 long plate adapter accessory.

SE100 ORDERING INFORMATION

Item# SE100 Includes: Polypropylene Washing Tank Plate Holders w/Handles & Dust Cover 2 sets

SE100 T ECHNICAL SPECIFICATIONS

Holders and Adapters Each holder can hold either 10 SE 600 series glass plates in the long direction, or 20 SE 250 glass plates in the short direction. An optional adapter can also be used to adapt a holder to hold 10 SE 260 or miniVE glass plates. Liquid Capacity of Tank 7 L to cover 18 x 16 cm plates (for the SE 400, SE 600 Chroma or SE 600) or 5 L to cover 10 x 8 cm and/or 10 x 10.5 cm plates (SE 250, SE 260, miniVE) Add more solution for larger plates, if required. Materials: Tank & Tank Lid: High-density Polyethylene Holders: Polypropylene Dust Covers: Vinyl Handles: Stainless Steel Operating Conditions: Maximum Temperature: 45 C

Long Plate Adapters - 2 pcs Item# SE103

Plate Holder w/Handle & Dust Cover Item# SE105

19

Glass Plate Washer & Storage Kit

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

SG Series

GRADIENT MAKERS

Gradient Makers

HOEFER SMALL VOLUME SG SERIES GRADIENT MAKERS are ideal for generating gradients of polyacrylamide, sucrose and cesium chloride. Choose from 15, 30, 50, or 100 ml sizes. Milled of heavy acrylic plastic with high-quality, leak-free Teflon valves. Flat base provides stability atop magnetic stirrers. Comes with a support rod for attaching to a ring stand. Takes Luer or 4-mm ID tubing. With included adapters, can also take 2-mm ID or 22-gauge tubing. SG 100 is ideal for the Hoefer Mighty Small Gel Casters SE 275 (for 4 gels) or SE 215 (for 10 gels).
SG 15, 30, 50, 100 ORDERING INFORMATION

SG 15 Gradient Maker SG 30 Gradient Maker SG 50 Gradient Maker (15 ml total volume) (30 ml total volume) (50 ml total volume) Item# SG15 Item# SG30 Item# SG50

SG 100 Gradient Maker (100 ml total volume) Item# SG100

All 4 Models Include: Gradient Maker w/Teflon Valves Luer Outlet Fitting Support Rod Adapter Barbed Fitting 22-gauge Needle

The 500 ml SG 500 GRADIENT MAKER is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base. A simple push-pull valve opens and closes the passage between the reservoir and mixing chambers. Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns. Ideal for use with the SE 615 Multiple Gel Caster.
SG 500 ORDERING INFORMATION

SG 500 Gradient Maker (500 ml total volume) Item# SG500 Includes: Gradient Maker w/Push-Pull Valve Wrap-and-Seal Hose Clamps 4 pcs

Gradient Makers

20

NUCLEIC ACID ELECTROPHORESIS

HE 33 MINI SUBMARINE ELECTROPHORESIS UNIT

SEE PAGE 22

HE 99X MAX SUBMARINE ELECTROPHORESIS UNIT

SEE PAGE 23

Nucleic Acid Electrophoresis

Submarine Agarose Units

Because of their semi-rigid shape and the regular phosphate charge distribution along their length, DNA fragments separate by size in simple electrophoresis systems. Agarose is the preferred gel matrix used, which is easy to cast and has a good size separation range. For separation of 100 bp to 20 kbp fragments, we recommend the Hoefer horizontal systems, HE 99X MAX SUBMARINE UNIT and HE 33 MINI SUBMARINE UNIT. Nucleic acids can also be separated in vertical acrylamide gels, which have a much higher resolving power than agarose gels but a narrower size range of separation. For vertical separations of small DNA fragments such as oligonucleotides up to 1000 bp, we recommend the HOEFER SE 300 miniVE or the SE 250 and SE 260 MIGHTY SMALL UNITS. For even further increased separation, we recommend the longer gels of the HOEFER SE 600 or SE 400 SERIES STANDARD VERTICAL ELECTROPHORESIS UNITS.

21 Nucleic Acid 1.1 Electrophoresis

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

HE 33 ORDERING INFORMATION
Max. Running Tray Size

7 x 10 cm

HE 33 Mini
Runs a 7 x 10 cm gel in as little as 5 minutes. Running tray accepts two combs for up to 32 samples.

NUCLEIC ACID ELECTROPHORESIS

Submarine Agarose Electrophoresis Unit

The HOEFER HE 33 MINI SUBMARINE AGAROSE UNIT is designed for very fast separations of DNA restriction fragments in agarose gels. To achieve excellent cooling, you simply fill the clear molded base with 50% ethylene glycol coolant solution, seal it permanently, and place it in a freezer or ice bath until ready to use.
HE 33 Mini Submarine Unit, Complete Item# HE33-8-1.5 Includes: Buffer Chamber Assembly Safety Lid w/High Voltage Leads Running Tray Casting Tray Foam Gaskets 4 pcs Spirit Level Comb, 1.5-mm thick, 8 wells Comb Back HE 33 Mini Submarine Unit, Basic Item# HE33B Order HE31A Comb and HE31-BK Comb Back separately. Includes: Buffer Chamber Assembly Safety Lid w/High Voltage Leads Running Tray Casting Tray Foam Gaskets 4 pcs Spirit Level

UV transparent running tray permits clear viewing of bands stained with ethidium bromide without handling the gel. Combs for forming sample wells are available with 8, 12, or 16 wells, in 1.0 or 1.5 mm thickness.

T ECHNICAL SPECIFICATIONS

Gel Dimensions: 7 x 10 cm Maximum Power Settings 500 V for 5 min or less, 500 mA, 15 W Maximum Buffer Volume: 250 ml Operating Conditions Maximum Temperature: 50 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (L x H x W): 24 x 7 x 13 cm (9.5" x 2.8" x 5.2") Safety Certifications: EN61010-1, UL61010A-1, CSA C22.2 1010.1, CE

HE 33 COMBS
# of Wells Thickness 1* 1.0 mm 1* 1.5 8 1.0 8 1.5 12 1.0 12 1.5 16 1.0 16 1.5 *1 prep/2 ref Well Width Qty. 44/6 mm 1 1 44/6 1 6.5 1 6.5 1 3.9 1 3.9 1 2.6 1 2.6 Item# HE31A-P-1.0 HE31A-P-1.5 HE31A-8-1.0 HE31A-8-1.5 HE31A-12-1.0 HE31A-12-1.5 HE31A-16-1.0 HE31A-16-1.5
Nucleic Acid Electrophoresis

22

ORDERING INFORMATION

NUCLEIC ACID ELECTROPHORESIS

HE 99X Max

Max. Running Tray Size

15 x 20 cm
HE 99X Max Submarine Unit, Complete Item# HE99X-15-1.5 Includes: Buffer Chamber Assembly Safety Lid w/High Voltage Leads Running Tray, 15 x 20 cm Casting Tray, 15 x 20 cm Foam Gaskets 4 pcs Spirit Level Comb, 1.5-mm thick, 15 wells Comb Back HE 99X Max Submarine Unit, Basic Item# HE99X Order Casting Kit, HE91A Comb and HE91-BK Comb Back separately. Includes: Buffer Chamber Assembly Safety Lid w/High Voltage Leads Spirit Level

Submarine Agarose Electrophoresis Unit

The HOEFER HE 99X MAX offers a variety of gel lengths and comb sizes so that you can design the gel you need for optimum resolution of DNA fragments. Casting and running trays are available for 15 x 20 cm, 15 x 15 cm, and 15 x 10 cm gels. Durable, leak-proof casting trays are easy to use. Cell-foam strips seal the ends securely. Self-locating/centering guides in the UV transparent running trays prevent gel sliding or floating. You can tailor gel configurations with a variety of combs. Comb depth is adjustable.

T ECHNICAL SPECIFICATIONS

Gel Dimensions: 15 x 20 cm, 15 x 15 cm, or 15 x 10 cm Maximum Power Settings: 200 V, 100 mA, 20 W Maximum Buffer Volume: 1.2 liter Operating Conditions: Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (L x H x W): 36 x 14 x 18.2 cm (14.2" x 5.5" x 7.2") Safety Certifications: EN61010-1, UL61010A-1, CSA C22.2 1010.1, CE

HE 99X COMBS
Order HE91-BK Comb Back separately. # of Wells Thickness 1* 1.5 mm 1* 3.0 10 1.5 10 3.0 15 1.0 15 1.5 15 3.0 20 1.0 20 1.5 20 3.0 30 1.0 *1 prep/2 ref Well Width Qty. 113/10 mm 1 1 113/10 1 9.7 1 9.7 1 7.1 1 7.1 1 7.1 1 4.7 1 4.7 1 4.7 1 3.0 Item# HE91A-P-1.5 HE91A-P-3.0 HE91A-10-1.5 HE91A-10-3.0 HE91A-15-1.0 HE91A-15-1.5 HE91A-15-3.0 HE91A-20-1.0 HE91A-20-1.5 HE91A-20-3.0 HE91A-30-1.0

23

Nucleic Acid Electrophoresis

Submarine Unit Accessories & Spare Parts


HE 33 Mini HE 99X Max
Gel Casting Kit Running Tray Casting Tray Foam Gaskets - 4 pc 15 x 20 cm Item# HE97X-20 15 x 15 cm Item# HE97X-15 15 x 10 cm Item# HE97X-10

NUCLEIC ACID ELECTROPHORESIS

Gel Casting Kit Running Tray Casting Tray Foam Gaskets - 4 pcs Item# HE47-10

Running Tray Item# HE42-10

Casting Tray Item# HE45-10 Running Tray 15 x 20 cm Item# HE92X-20 15 x 15 cm Item# HE92X-15 15 x 10 cm Item# HE92X-10 Casting Tray with Foam Gaskets - 4 pc 15 x 20 cm Item# HE95X-20 15 x 15 cm Item# HE95X-15 15 x 10 cm Item# HE95X-10

Spirit Level Item# SER11

Foam Gaskets (4 pcs) Item# HE48

Top Fill Plug Kit (4 pcs) Item# HE38TP

Comb Back + 2 screws Item# HE31-BK

Comb Back + 2 screws Item# HE91-BK

Spirit Level Item# SER11

Foam Gaskets (4 pcs) Item# HE98X

Buffer Chamber Assembly Item# HE30

Buffer Chamber Assembly Item# HE90X

Lid w/High Voltage Leads Item# HE96X Lid w/High Voltage Leads Item# HE36 High-Voltage Leads (2 pcs) Item# SE6056-HV Electrode Replacement Kit (not pictured) Item# HE99XRK-1 High-Voltage Leads (2 pcs) Item# SE6056-HV
Nucleic Acid Electrophoresis

Electrode Replacement Kit (not pictured) Item# HE39

24

PROTEIN BLOTTING
25
Protein Blotting

Hoefer pioneered the vertical format for electrophoresis separations and continues to offer innovative designs and a variety of choices.

PROTEIN BLOTTING

Protein Blotting
TE Series Transfer Systems
Electrophoretic transfer of proteins is a much faster procedure than simple capillary or contact blotting methods. By directing electrical current through a transfer stack, blotting rates can be greatly accelerated. Assays performed on transfer membranes are sensitive and require only small amounts of material. This is critical when the species of interest is present in low concentrations or represents a minor fraction of the total sample. Moreover, since antibodies and other affinity reagents are often expensive, this can greatly reduce cost. HOEFER TE SERIES TRANSFER TANKS and SEMI-DRY TRANSFER UNITS use electrophoresis to deliver excellent transfers of proteins and nucleic acids from polyacrylamide or agarose gels onto nitrocellulose, PVDF or nylon membranes.

TE 22 MIGHTY SMALL TRANSFER TANK

SEE PAGE 29

TE 42 TRANSFER TANK

SEE PAGE 30

TE 62 TRANSFER TANK

SEE PAGE 30

TE 70X SERIES SEMI-DRY TRANSFER UNITS

SEE PAGES 27 & 28

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Protein Blotting

26

ORDERING INFORMATION

PROTEIN BLOTTING

Widest range of systems to suit all your western blotting needs.

Max. Gel Size

14 x 16 cm

TE 70X & TE 70XP


Semi-Dry Transfer Units

TE 70X Semi-Dry Transfer Unit Item# TE70X Includes: Molded Base w/Pt-Nb Anode & High Voltage Leads Hinged Lid w/Stainless Steel Cathode Blotter Paper 25 sheets Porous Cellophane 50 sheets Mylar Masks 2 pcs TE 70XP Semi-Dry Transfer Unit Item# TE70XP Includes: Molded Base w/Pt-Nb Anode & Internal Power Supply Hinged Lid w/Stainless Steel Cathode Blotter Paper 25 sheets Porous Cellophane 50 sheets Mylar Masks 2 pcs Accessories: Mylar Masks 4 pcs, Item# TE74 Blotter Paper 25 sheets, Item# TE76-1416 Porous Cellophane 50 sheets, Item# TE73

TE 70X

The TE 70X and TE 70XP SEMI-DRY TRANSFER UNITS use minimal buffer to transfer proteins and nucleic acids from polyacrylamide and agarose gels in less than an hour. The TE 70XP has the added convenience of a built-in power supply. Units accept gels up to 14 x 16 cm, or two SE 260 mini gels side-by-side. Transfer two polyacrylamide gels in a stack. Economical to use. Require only a small amount of buffer. Require minimal current. Do not generate the excessive heat that can dry out the transfer stack and halt transfer or damage transfer units. Durable platinum-niobium and stainless steel electrodes assure contamination-free transfers. TE 70XP includes an intelligent built-in power supply which automatically monitors the transfer status, stopping the transfer before the stack overheats.

T ECHNICAL SPECIFICATIONS

Gel Dimensions: up to 14 x 16 cm Maximum Power Settings 50 V, 200 mA, 10 W Operating Conditions Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): 38 x 46 x 9 cm (15" x 18" x 3.5") Safety Certifications EN610101 CE

27

Protein Blotting

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

ORDERING INFORMATION

PROTEIN BLOTTING

Max. Gel Size

21 x 26 cm

TE 77X & TE 77XP


Produce uniform and even transfer results quickly. Require only a small amount of buffer.

Semi-Dry Transfer Units

TE 77X Semi-Dry Transfer Unit Item# TE77X Includes: Molded Base w/Pt-Nb Anode & High Voltage Leads Hinged Lid w/Stainless Steel Cathode Blotter Paper 25 sheets Porous Cellophane 50 sheets Mylar Masks 2 pcs TE 77XP Semi-Dry Transfer Unit Item# TE77XP Includes: Molded Base w/Pt-Nb Anode & Internal Power Supply Hinged Lid w/Stainless Steel Cathode Blotter Paper 25 sheets Porous Cellophane 50 sheets Mylar Masks 2 pcs Accessories: Mylar Masks 4 pcs, Item# TE78 Blotter Paper 25 sheets, Item# TE76 Porous Cellophane 50 sheets, Item# TE73

The HOEFER TE 77X and TE 77XP SEMI-DRY TRANSFER UNITS have the same efficient design and outstanding features as the TE 70X and TE 70XP, but accommodate larger gels. Units accept gels up to 21 x 26 cm, or four SE 260 minigels side-by-side. Transfer two polyacrylamide gels in a stack.

Require minimal current. Do not generate the excessive heat that can dry out the transfer stack and halt transfer or damage the unit. Durable platinum-niobium and stainless steel electrodes assure contamination-free transfers. TE 77XP includes an intelligent built-in power supply which automatically monitors the transfer status, stopping the transfer before the stack overheats.

T ECHNICAL SPECIFICATIONS

Gel Dimensions: up to 21 x 26 cm Maximum Power Settings 50 V, 450 mA, 22.5 W Operating Conditions Maximum Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): 38 x 46 x 9 cm (15" x 18" x 3.5") Safety Certifications EN610101 CE

TE 77XP

Protein Blotting

28

PROTEIN BLOTTING

TE 22
The compact TE 22 MIGHTY SMALL TRANSFER TANK quickly and efficiently transfers proteins and nucleic acids from small gels to nylon, nitrocellulose or PVDF membranes. Transfers as many as four small gels, up to 9 x 10 cm in less than an hour. Built-in alumina-covered cooling channel provides excellent temperature control with no more than a 5 C temperature increase during a typical run. Superior design provides a uniform electric field the key to even transfers. Cassettes are easy to load and handle. No gel distortion. Grooves inside the unit hold cassettes in place, applying equal pressure across the gel.
T ECHNICAL SPECIFICATIONS

Accessories & Spare Parts

Mighty Small Transfer Tank


ORDERING INFORMATION

Capacity up to four 9 x 10 cm gels (SE 260-sized) Maximum Power Settings 100 V, 500 mA, 50 W Operating Conditions Max. Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): 14 x 24 x 16.5 cm (5.5" x 9.5" x 6.5") Safety Certifications EN61010-1, UL3101-1, CSA C22.2 1010.1, CE

Electrode Panel Item# TE23


Max. Gel Size

9 x 10 cm

1-4 GELS

TE 22 Mighty Small Transfer Tank Item# TE22 Includes: Lower Chamber w/Cooling Channel Safety Lid w/High Voltage Leads Electrode Panels 2 pcs Cassettes 4 pcs Foam Sponges, 6-mm Thick 4 pcs Foam Sponges, 3-mm Thick 8 pcs Cassette Hook Blotter Paper 25 sheets Cassette w/Sponges Item# TE24

Foam Sponges 6-mm Thick 4 pcs Item# TE25 3-mm Thick 4 pcs Item# TE25F-1/8

Lower Chamber w/Cooling Channel Item# TE27

Safety Lid w/High Voltage Leads Item# TE29 Replacement High Voltage Leads Item# SE6056-HV

29

Protein Blotting

Blotter Paper, 50 sheets (Not pictured) Item# TE26 Repair Kit, Fittings (Not Pictured) Item# TE22RK-1

TE 62 & TE 42

Accessories & Spare Parts


Electrode Panel, Black Item# TE43BK

PROTEIN BLOTTING

Transfer Tanks

Electrode Panel, Grey Item# TE43GY

Cassette w/Sponges Item# TE44H

Foam Sponges

The TE 62 TRANSFER TANK quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nitrocellulose, PVDF or nylon membranes. A cooling chamber built into the base of the unit provides efficient temperature control for optimal transfer results. Transfers up to four 15 x 21 cm gels or as many as sixteen 7 x 10 cm mini-gels. Built-in alumina-covered cooling channel together with external cooling waterbath (see page 36) ensures consistent transfers. Perform transfers in one hour. Easy-to-load cassettes hold gels evenly no gel distortion.

TE62 ORDERING INFORMATION

6-mm Thick 4 pcs Item# TE45F 3-mm Thick 4 pcs Item# TE45F-1/8

Max. Gel Size

15 x 21 cm

1-4 GELS

Item# TE62 Includes: Lower Chamber w/Cooling Channel Safety Lid w/High Voltage Leads Electrode Panels 2 pcs Cassettes 4 pcs Foam Sponges, 6-mm Thick 4 pcs Foam Sponges, 3-mm Thick 8 pcs Cassette Hook Blotter Paper 25 sheets

Lower Chamber w/Cooling Channel, for TE 62 Item# TE67

The TE 42 TRANSFER TANK differs from the TE 62 only in the cooling system. Use the optional TE 47 Heat Exchanger and external cooling waterbath to control buffer temperature during transfer. Transfers up to four 15 x 21 cm gels at once without cooling, or up to two gels at once with optional cooling
T ECHNICAL SPECIFICATIONS

TE42 ORDERING INFORMATION

Lower Chamber, for TE 42 Item# TE56

Max. Gel Size

15 x 21 cm

1-4 GELS

Capacity TE 62: up to four 15 x 21 cm gels TE 42: up to four 15 x 21 cm gels (without cooling); up to two 15 x 21 cm gels (with cooling) Maximum Power Settings: 100 V, 2 A, 200 W Operating Conditions Max. Temperature: 45 C Environmental Operating Conditions: Indoor Use: 4 - 40 C, Humidity: up to 80%, Altitude: up to 2000 m Unit Dimensions (W x H x D): SE 600: 28 x 16.5 x 32 cm (11" x 6.5" x 12.5") SE 660: 28 x 13 x 30.5 cm (11" x 5.1" x 12") Safety Certifications EN61010-1, UL3101-1, CSA C22.2 1010.1 and CE

Item# TE42 Includes: Lower Chamber Safety Lid w/High Voltage Leads Electrode Panels 2 pcs Cassettes 2 pcs Foam Sponges, 6-mm Thick 2 pcs Foam Sponges, 3-mm Thick 4 pcs Cassette Hook Blotter Paper 25 sheets

Safety Lid w/High Voltage Leads Item# TE49

Replacement High Voltage Leads Item# SE6056-HV

Blotter Paper 50 sheets Item# TE46

Not Pictured Dacron Sponges, 6-mm Thick 2 pcs Item# TE45 Heat Exchanger Item# TE47

Protein Blotting

30

IMMUNOBLOTTING

IMMUNOBLOT XL SEE PAGE 32

Immunoblotting
ImmunoBlot and Deca-Probe Blotting Manifolds
Transfer of proteins or nucleic acids, fractionated by gel electrophoresis, to an immobilizing membrane surface increases the sensitivity of a wide range of detection methods, reduces time of analysis, and makes sequential probing possible. In particular, it has made possible the use of immunological procedures that are not practical to carry out on a gel or in a tube or plate. The HOEFER IMMUNOBLOT AND IMMUNOBLOT XL and HOEFER PR 150 DECAPROBE make it possible to assay the material on a transfer membrane with different antigens or antibody probes simultaneously without cutting the membrane into strips. The membrane remains intact to preserve the correspondence between different lanes or positions on the gel. The HOEFER PR 648 SLOT BLOT MANIFOLD provides a simple, convenient method to immobilize unfractionated samples to a membrane, allowing screening of recombinant clones, DNA, RNA and proteins.
PR 150 DECA-PROBE

SEE PAGE 33

PR 648 SLOT BLOT BLOTTING MANIFOLD

SEE PAGE 33

31 1.1 Immunoblotting

ORDERING INFORMATION

ImmunoBlot XL Item# PR645 Includes: Top Plate, 45 Channels Bottom Plate Screws - 2 pcs Washing Manifold - 2 pcs Tubing - 2 pcs Luer Manifold Connectors - 4 pcs Sealing Pads - 5 pcs ImmunoBlot Item# PR625 Includes: Top Plate, 25 Channels Bottom Plate Screws - 2 pcs Washing Manifold - 2 pcs Tubing - 2 pcs Luer Manifold Connectors - 4 pcs Sealing Pads - 5 pcs

ImmunoBlot & ImmunoBlot XL


Blotting Manifolds

IMMUNOBLOTTING

T ECHNICAL SPECIFICATIONS

Capacity ImmunoBlot XL: 45 channels, 140 l per channel ImmunoBlot: 25 channels, 250 l per channel Channel Dimensions 13 cm long, spread out over 13 cm width; accommodates SE 600 and SE 400 gels. Operating Conditions Maximum Temperature: 45 C Unit Dimensions (W x H x D): 20 x 20 x 8 cm (7.9" x 7.9" x 3.1")

COMBS

# of Wells 9 12 25

Thickness 1.0 mm 1.0 1.0

Item# PR511-9-1.0 PR511-12-1.0 PR511-25-1.0

Use with ImmunoBlot XL ImmunoBlot ImmunoBlot

ImmunoBlot blotting systems are simple and convenient tools for all immunoblotting techniques like Western blotting, antibody screening and DNA hybridizations. The ImmunoBlot XL has 45 sample channels. Each channel holds up to 140 l of antibody or probe solution. The ImmunoBlot has 25 sample channels. Each channel holds up to 250 l. Greater volume per channel enables high sensitivity detection of low affinity and low titer antibodies. Results appear on a single membrane for convenient side-by-side comparison. Optional washing manifold enables simultaneous washing of all channels. Can be used with all conventional detection methods including enzymatic, radioactive and chemiluminescence. Optional gel combs precisely line up electrophoresis lanes with ImmunoBlot channels.

Accessories & Spare Parts


Sealing Pads 10 pcs, Item# PR630-31 Washing Manifold Kit, Item# PR630-32 Includes: Washing Manifold - 2 pcs Tubing - 2 pcs Luer Manifold Connectors - 4 pcs O-Ring Seals 2 pcs, Item# PR630-33 Luer Manifold Connectors 4 pcs, Item# PR630-34 Clamp Screw, Item# PR630-36 Top Plate, for PR 645, Item# PR645T Top Plate, for PR 625, Item# PR625T Bottom Plate, for either unit, Item# PR630-37

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Immunoblotting

32

IMMUNOBLOTTING

PR 150 Deca-Probe
T ECHNICAL SPECIFICATIONS

Incubation Manifold

Capacity: 10 channels, 0.5 2.0 ml per channel Channel Dimensions: 8.8 cm long, spread out over 8.8 cm width; accommodates SE 260 and miniVE gels. Operating Conditions: Maximum Temperature: 45 C Unit Dimensions (W x H x D): 13 x 13 x 2 cm (5.1" x 5.1" x 0.8")

Accessories & Spare Parts

Sample Gasket Item# PR151

ORDERING INFORMATION

The HOEFER PR 150 DECA-PROBE INCUBATION MANIFOLD has 10 incubation chambers for screening antibody preparations or probe dilutions. The Deca-Probe has all of the features of the ImmunoBlot in a smaller format. The Deca-Probe is excellent for antibody screening and Western blotting of minigels. Individually isolated liquid-tight chambers allow you to use different detection methods or antibodies side-by-side. Screen 10 samples individually on a single 8.8 x 8.8 cm transfer membrane for side-by-side lane comparisons. Screen up to 10 different antibody preparations against a single antigen mixture or one antibody preparation against as many as 10 different antigen mixtures on a single, intact membrane. Economical to use. Requires minimal volume of reagents. Reliable. No worry about artifacts caused by incomplete exposure or mixing.

Deca-Probe Incubation Manifold Item# PR150 Includes: Pressure Plate Sample Plate w/10 Sample Chambers Teflon Screws 6 pcs Sample Gasket Pressure Gasket

Pressure Gasket Item# PR152

COMBS

# of Wells 10 10 10

Thickness 0.75 mm 1.0 1.5

Item# SE211A-10-.75 SE211A-10-1.0 SE211A-10-1.5

Teflon Screws 4 pcs Item# PR153

PR 648 Slot Blot

The HOEFER SLOT BLOT MANIFOLD offers a simple, convenient method for screening recombinant clones, DNA, RNA and proteins. Screens as many as 48 samples quickly and easily. Each well holds up to 1 ml sample volume. Slot dimensions are 6 x 0.8 mm. Numbered slots facilitate accurate sample application. A raised lip around each slot creates an effective seal and prevents crosscontamination between wells. Alignment pins ensure quick and easy assembly. Attaches easily to a vacuum pump or water aspirator.

ORDERING INFORMATION

Blotting Manifold

Slot Blot Manifold Item# PR648 Includes: Top Block Membrane Support Block w/ O-ring Bottom Block w/ Connector QuickFit Connector w/ O-ring Clamping Screws 6 pcs Membrane Cutting Template

T ECHNICAL SPECIFICATIONS

Capacity: 1 ml sample volume per slot Slot Dimensions: 6.0 x 0.8 mm; numbered Membrane Size: 11.5 x 8 cm Operating Conditions: Maximum Temperature: 45 C Unit Dimensions (W x H x D): 15.5 x 12 x 8 cm (6.1" x 4.7" x 3.1")

33

Immunoblotting

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Blotting Membranes
Hoefer introduces high quality membranes in convenient formats for your blotting needs. Nitrocellulose Membranes: Ideal for Western Blotting and other Immunoblotting techniques. Available in 0.2 m and 0.45 m thickness in rolls and convenient pre-cut sheets.

BLOTTING MEMBRANES

for Western, Northern and Southern Blotting

BSA protein binding capacity of 160 g/cm 2. Will bind a wide range of fragment sizes and can still be easily blocked for low background. High wick rates (lateral flow). Substances move quickly through the membrane. Uniform pore structure provides consistent flow and diffusion rates. Superior strength. Withstands aggressive handling or can be used with automated equipment without breaking or tearing. Stringent quality tests ensure lot-to-lot consistency and produce dependable results every time.

Nylon Membranes: Ideal for Northern and Southern Blotting. Available in 0.45 m pore size in rolls and convenient pre-cut sheets. Multiple reprobings possible. Inherently charged. Enhances nucleic acid binding even under alkaline conditions. Supported. Has added strength and durability, preventing distortion or contamination in multiple reprobings. Greater binding capacity, 450 g/cm 2. Can bind a wide range of fragment sizes and still be easily blocked for low background. Excellent signal. Retains DNA better, resulting in a strong signal using smaller quantities of DNA. Stringent quality tests ensure lot-to-lot consistency and produce dependable results every time.

PVDF (Polyvinylidene Fluoride) Membranes: Ideal for use in protein binding applications such as western blots, solid phase assays and immunoblotting procedures. Available in 0.45 m pore size in a 3 meter roll. Superior strength. Can withstand aggressive handling or be used with automated equipment without breaking or tearing. Low extractables. Ensures tests will be clean with consistent results. Exceptional sensitivity detects low-level components. Hydrophobic. Resists water. High binding capacity. Binds a wide range of fragment sizes. Lot-to-lot consistency. Quality checks ensure consistent binding for dependable results every time.

ORDERING INFORMATION

Nitrocellulose membrane, 0.22 m pore size, 30 cm x 3 m roll Item# GM-NC22 Nitrocellulose membrane, 0.45 m pore size, 30 cm x 3 m roll Item# GM-NC45 Nitrocellulose membrane, 0.45 m pore size 8 x 9.5 cm, 10/pack Item# GM-NC45-89 Nitrocellulose membrane, 0.45 m pore size 16 x 16 cm , 10/pack Item# GM-NC45-1616 Nitrocellulose membrane, 0.45 m pore size 23.5 x 20 cm, 10/pack Item# GM-NC45-2320 Nylon membrane, 0.45 m pore size, 30 cm x 3 m roll Item# GM-NY45 Nylon membrane, 0.45 m pore size, 15 x 20 cm, 10/pack Item# GM-NY45-1520 PVDF membrane, 0.45 m pore size, 30 cm x 3 m roll Item# GM-PV45

Blotting Membranes

34

FILTRATION

FH 225V Filtration Manifold


The HOEFER FH 225V TEN-PLACE FILTRATION MANIFOLD holds ten 25-mm diameter filters in place with simple chimney weights. The manifold rests on a clear, chemical- resistant PVC collection box. The Filtration Manifold is recommended for detection of pathogens like Giardia in water analysis. Holds ten individual stainless steel chimney weights, each with a capacity of 13 ml. Precision-machined chimney weights, made of type 316 solid stainless steel, provide a secure, liquid-tight seal. No need for clamps or O-rings. Clear PVC collection box acts as a vacuum chamber, exerting equal vacuum on all ten positions. With the optional FH 240 rack, individual filtrates can also be collected into test tubes or 27-mm scintillation vials. Individual on/off valves allow you to regulate each position separately. PVC collection box is resistant to trichloroacetic acid (TCA).
T ECHNICAL SPECIFICATIONS ORDERING INFORMATION

Ten-Place Filtration Manifold

Filter Size: 25 mm diameter circles Sample Volume: 13 ml Operating Conditions: Maximum Temperature: 45 C Unit Dimensions (W x H x D): 32.4 x 20.3 x 15.2 cm (12.8" x 8" x 6")

Ten-Place Filtration Manifold Item# FH225V Includes: Filter Holding Manifold w/Vacuum Gauge 10 Individual Teflon Valves PVC Collection Box w/Gasket Tubing Connector Stainless Steel Chimney Weights 10 pcs

Accessories & Spare Parts

PVC Collection Box w/Gasket & Tubing Connector Item# FH251

Gasket, 100 cm Item# FH2208

Filter Holding Manifold w/Vacuum Gauge & Valves Item# FH225VM

Chimney Weight Item# FH252-25

35

Filtration

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

T ECHNICAL SPECIFICATIONS

Working Temperature Range: -20 to +100 C Resolution: 0.1 C Heater Wattage: 1000 W at 115 V, 2000 W at 230 V Cooling Capacity (ethanol medium) (W): 200 120 70 20 at temperature (C): +20 0 -10 -20 Refrigerant: R134a Operating Conditions Environmental Operating Conditions: Indoor Use: 5 - 40 C Humidity: up to 80% for temperatures up to 31 C; linear decrease to 50% at 40 C Altitude: up to 2000 m Unit Dimensions (W x H x D): 20 x 56 x 36 cm (7.9" x 22" x 14.2") Weight: 22 kg Safety Certifications: EN61010-1, CE

CIRCULATION BATH

RCB-20

Refrigerated & Heated Circulation Bath

ORDERING INFORMATION

RCB-20 Refrigerated & Heated Circulation Bath 115 VAC, 60 Hz - Item# RCB20-115V 230 VAC, 50 Hz - Item# RCB20-230V 100 VAC, 60 Hz - Item# RCB20-100V Each Includes: Refrigerated/Heated Circulation Bath Tygon Tubing, 3/8 ID two 3-foot lengths QuickFit Connectors 2 sets Pump Connector Barbed Fittings 2 pcs Accessories & Spare Parts Tubing Kit - Item# RCB21 (Tygon Tubing, 3/8 ID two 3-foot lengths and QuickFit Connectors 2 sets)

The HOEFER RCB-20 REFRIGERATED/HEATED CIRCULATION BATH provides efficient temperature control for all types of electrophoresis and transfer techniques. Provides temperature control in the -20 to +100 C range, regulated to 0.1 C. Compact footprint. The RCB-20 is just 20 cm wide x 36 cm deep. Cooling capacity of 200 W at 20 C. Delivers heat with a 1000 W heater (115 VAC). Designed for continuous use, the RCB-20 has a 15 liter per minute circulation pump. Appropriate for use with these other Hoefer instruments: the SE 250, SE 260, Chroma, SE 600 and SE 660 vertical electrophoresis units; and the TE 22,TE 42 and TE 62 tank transfer units.

QUALITY CONTROL

Hoefer is passionate about electrophoresis, and has been a pioneering force since the early 1970s. That dedication to assisting life scientists begins long before our instruments reach your laboratory. We put every component of a Hoefer designed and manufactured product through the highest standards in quality control to ensure that the finished product is built to last and last. You can be assured our products are the best that you can buy.

Circulation Bath

36

POWER SUPPLIES

EV 265 Consort

Power Supply

ORDERING INFORMATION

EV 265 Consort Power Supply, 115 VAC Item# PS265-115V EV 265 Consort Power Supply, 230 VAC Item# PS265-230V

T ECHNICAL SPECIFICATIONS

Voltage: 600 V Current: 500 mA Power: 150 W Parameter Range: 1 - 100% of full scale Timer: 99:59 h

The EV 265 CONSORT POWER SUPPLY is ideal for electrophoresis and semi-dry blotting techniques. The electrical leads from all HOEFER gel and blot units are fully compatible without the need for adapters. Maximum output: 600 V, 500 mA, 150 W. Constant voltage, constant current, or constant power mode with automatic crossover. Programmable step function. Stores and recalls as many as 9 sets of frequently used parameters. Timer or volt-hour controlled with alarm. Automatic recovery after power failure. Precise, reproducible settings and readouts in single-unit increments (1 V, 1 mA, 1 W, and 1 min). Manual programming mode allows temporary changes to parameters without interrupting the run. Integral data-logger periodically stores output values including program number and step for up to 3600 points.

Volt-Hours: 0 - 99.99 kVh Display: LCD, 2 x 16 characters Resolution: 1 V, 1 mA, 1 W Programs: 9 x 9 set of parameters Outputs: 4 in parallel, 4 mm sockets Min. Load Resistance: 30 ohm Ground Leakage Detection: 500 A No Load Detection: programmable Overload Detection: Yes Data-Logging: 3600 values RS232: 9600 b/s Dimensions (W x D x H): 31 x 26 x 15 cm Weight: 5 kg Safety Certification: CE

37

Power Supplies

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

PS 2A200
Power Supply

POWER SUPPLIES

The high current PS 2A200 POWER SUPPLY is ideal for all types of electrophoresis blotting applications and for short electrophoresis runs. Maximum output: 200 V, 2000 mA, 200 W. Constant voltage, constant current, or constant power mode with automatic crossover. Programmable step function. Stores and recalls 3 protocols. Choose from 4 timer options: 1) Continuous run 2) Set time run 3) Set time run followed by a hold at 5 volts 4) Set volt-hour run. Precise, reproducible settings and readouts in single-unit increments (1 V, 1 mA, 1 W, and 1 min). Prints a log of run parameters through an RS-232 port. Designed for high current applications such as tank blotting. Automatic recovery after power failure. Compact design saves bench space.

T ECHNICAL SPECIFICATIONS

Maximum Output: 200 V, 2000 mA, 200 W, automatic crossover Timer: 0 99:59 hr or 1 9999 Vhr Output: 2 pairs of sockets, in parallel, 4-mm, HV, recessed Operating Conditions: Environmental Operating Conditions: Indoor Use: 5 - 40 C Humidity: 0 - 90% non-condensing Open Circuit Detection: Open circuit protection for resistance over 300K Ohms Unit Dimensions (W x H x D): 11 x 28 x 22 cm (8.6" x 4.3" x 10.7") Weight: 2.2 kg Safety Certifications: EN61010-1, UL 3101-1, CSA22.2 1010-1, CE

ORDERING INFORMATION

PS 2A200 Power Supply, 90-265 VAC, 47-63 Hz Item# PS2A200

Power Supplies

38

Gel Casting Reagents


ELECTROPHORESIS REAGENTS

Caliber

Acrylamide

Ammonium Persulfate
(APS) ACS Reagent Grade (NH4)2S2O8 Formula Weight: 228.20 Product Specifications Form: Off-white crystalline powder Assay: 98.0% Residue on Ignition: 0.05% Insoluble Matter: 0.005% Titratable Free Acid: 0.04 meq/g Chloride (Cl): 0.001% Heavy Metals (Pb): 0.005% Iron (Fe): 0.001% Manganese (Mn): 0.5 ppm Solubility (2 M, H20): Complete, clear and colorless CAS # 7727-54-0 Application: A high speed initiator used in polyacrylamide gel electrophoresis. Use: PAGE Quantity: 10 g Item# GR152-10

High Quality Electrophoresis Reagents

Hoefer Caliber Electrophoresis Reagents deliver consistently outstanding results. A high standard of purity, rigorous analytical procedures, and carefully controlled manufacturing techniques ensure that Hoefer Caliber reagents are the best quality buffer, gel casting, gel staining and sample preparation chemicals available. A complete range of chemicals for electrophoresis. Free from impurities that interfere with polymerization. Manufactured to stringent specifications. Functionally tested by batch to ensure reproducibility. Pure powder or premixed ready-to-use solutions, to meet your labs needs and convenience.

MB Grade CH2=CHCONH2 Formula weight: 71.08 Product Specifications Form: White/clear crystalline powder Assay: 99.9% Identity: by IR A (410 nm, 35%, 1 cm): 0.02 A (290 nm, 1%, 1 cm): 0.1 A (280 nm, 7 mM, 1 cm): 0.04 A (252 nm, 7 mM, 1 cm): 1.20 Insoluble matter (H2O): 0.005% Insoluble matter (MeOH): 0.005% pH (10%, 0.1 N NaCl): 6.0 7.0 Melting Point: 84 1 C Acrylic Acid: 0.001% Conductivity (35%): 2 mhos/cm Iron (Fe): 1 ppm Lead (Pb): 1 ppm Gel Performance: Passes test CAS # 79-06-1 Use: PAGE Quantity: 1 kg Item# GR141-1

N,N'-Methylene-bis-Acrylamide
(bis-Acrylamide) MB Grade (CH2=CHCONH)2CH2 Formula Weight: 154.17 Product Specifications Form: White crystalline powder Assay: 99.9% Identity: By IR A (290 nm, 1%, 1 cm): 0.2 pH (1%, H2O): 5.5 Acrylic acid: 0.02% Lead (Pb): 1 ppm Gel Performance: Passes test CAS # 110-26-9 Application: Cross-linking agent used in forming polyacrylamide gels. The porosity of the gel is determined in part by the degree of cross-linking. Most protein gels use a ratio of 0.9 g of cross-linker to 30 g of acrylamide. Use: PAGE Quantity: 100 g Item# GR142-100

Agarose
MB Grade Product Specifications Form: Granular, free-flowing powder EEO (-mr): 0.05 - 0.13 Gel Point (1.5%): 36.0 1.5 C Remelt Point (1.5%): 88.0 1.5 C Gel Strength (1%): 1200 gm/cm2 Gel Strength (1.5%): 2500 gm/cm2 Moisture: 7% Turbidity (1.5%): 40 Np Residue on Ignition: 0.4% Sulfate (SO4): 0.14% CAS # 9012-36-6 Genetic Performance Tests: DNase: Passes test RNase: Passes test Restriction Enzyme Digestion: Passes test Ligation: Passes test DNA Binding: Passes test Blotting: Passes test Gel Background: Passes test DNA Resolution: Passes test Separation Guidelines: Separation Range (bp) in 1X TBE % Agarose in 1X TAE 0.6 20,000 - 1,000 15,000 - 1,000 0.8 10,000 - 500 12,000 - 500 1.0 7,000 - 250 8,000 - 300 1.2 5,000 - 200 6,000 - 200 1.5 3,000 - 100 3,500 - 100 2.0 2,000 - 50 2,000 - 50 Applications: 1. Separation of DNA or RNA fragments. 2. PCR analysis. 3. Recovery of separated DNA or RNA fragments from gel. 4. Blotting applications such as Southerns and Northerns. 5. Protein analysis such as immunodiffusion, rocket assays and immunoelectrophoresis. Use: DNA/RNA detection Quantity: 500 g Item# GR140-500

N,N,N',N'Tetramethylethylenediamine
(TEMED) (CH3)2NCH2CH2N(CH3)2 Formula Weight: 116.20 Product Specifications Form: Colorless to pale yellow liquid Assay: 98% A (290 nm, 1%, 1 cm): 0.1 Density (20 C): 0.77 0.01 Refractive Index (20 C): 1.419 0.001 CAS # 110-18-9 Application: Catalyzes the formation of free radicals by ammonium persulfate and accelerates the polymerization of acrylamide and bisacrylamide. Use: PAGE Quantity: 25 g Item# GR151-25

GEL CASTING KITS SEE PAGE 24

39 Electrophoresis 1.1 Reagents

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FAX 1.415.920.4494

Sample Preparation And Loading Reagents


Protein Determination Reagent
Description: One bottle of Protein Determination Reagent is sufficient for 500 standard assays or 2500 microassays. Application: This protein dye reagent allows rapid and simple determination of the protein content of various solutions. This reagent is based on the Bradford(1,2) protein assay method and is supplied as a five-fold concentrate. It can be used either in a standard assay procedure for protein concentrations between 100 g/ml and 1 mg/ml or in a microassay procedure for concentrations between 10 g/ml and 100 g/ml. The assay procedure consists of mixing the reagent with sample and blank, incubating (usually 5 - 20 min), and measuring the absorbance of the sample and blank at 595 nm. The assay procedure is supplied with every bottle. References: 1. BRADFORD, M. M. (1976) Anal. Biochem. 72, 248 - 254. 2. BRADFORD, M. M. AND W. L. WILLIAMS (1977) U.S. Patent No. 4,023,933. Use: PAGE, IEF, 2D Quantity: 500 ml Item# GR133-500

EDTA, 0.5 M Solution


MB Grade Description: 0.5 M solution of EDTANa2 in high purity dH2O, pH 8.0. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. pH: 8.0 0.1 Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Storage: Ambient Application: Chelating agent used in many enzyme buffers and, at higher concentrations, as an enzyme inactivator. Use: PAGE, DNA/RNA detection Quantity: 100 ml Item# GR123-100

Glycerol
MB Grade HOCH2CH(OH)CH2OH Formula Weight: 92.09 Product Specifications Form: Clear, colorless, viscous liquid Assay: 99.5% Identity: by IR Specific Gravity (25 C): 1.260 1.263 A (230 nm, 100%, 1 cm): 0.15 A (260 nm, 100%, 1 cm): 0.06 A (280 nm, 100%, 1 cm): 0.05 Color (APHA): 10 Neutrality: Passes test Residue on Ignition: 0.005% Moisture: 0.5% Acrolein, Glucose and Ammonium Compounds: Passes test Fatty Acid Esters: 0.2 meq/100 gm Silver Reducing Substances: Passes test Readily Carbonizable Substances: Passes test Chlorinated Compounds: 0.003% Chloride (CI): 0.001% Sulfate (SO4): 0.002% Arsenic (As): 1.5 ppm Calcium (Ca): 5 ppm Iron (Fe): 5 ppm Magnesium (Mg): 1 ppm Mercury (Hg): 10 ppm Zinc (Zn): 2 ppm Heavy Metals (Pb): 2 ppm DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test CAS # 56-81-5 Use: PAGE, density agent Quantity: 1 L Item# GR124-1

Sucrose
MB Grade C12H22O11 Formula Weight: 342.30 Product Specifications Form: White/clear crystalline powder [ ]D25: +66.3 to +66.8 (c=26 in H2O) A (260 nm, 1 M, 1 cm): 0.150 A (280 nm, 1 M, 1 cm): 0.125 Insoluble Matter: 0.005% Loss on Drying: 0.03% Residue on Ignition: 0.01% Titratable Acid: 0.0008 meq/gm Invert Sugar: 0.05% Chloride (Cl): 0.005% Sulfate and Sulfite (as SO4): 0.005% Arsenic (As): 1 ppm Barium (Ba): 1 ppm Cadmium (Cd): 1 ppm Calcium (Ca): 5 ppm Copper (Cu): 1 ppm Iron (Fe): 1 ppm Lead (Pb): 1 ppm Magnesium (Mg): 1 ppm Manganese (Mn): 1 ppm Zinc (Zn): 1 ppm DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test CAS # 57-50-1 Use: PAGE, density agent Quantity: 1 kg Item# GR129-1

ELECTROPHORESIS REAGENTS

Bromophenol Blue, Sodium Salt


ACS Reagent Grade C19H9Br4NaO5S Formula Weight: 691.95 Product Specifications Form: Powder Clarity of Solution: Passes test Visual Transition Interval: pH 3.0 - Yellow pH 4.6 - Blue CAS # 62625-28-9 Applications: Used as a tracking dye in agarose and acrylamide gel electrophoresis. Used in the stop solution in DNA sequencing kits. Use: PAGE, DNA/RNA detection Quantity: 10 g Item# GR120-10

Sodium Dodecyl Sulfate (SDS)


CH3(CH2)11OSO3Na Formula Weight: 288.38 Product Specifications Form: White crystalline flakes Identity: by IR Assay: 95% (dry basis) Solubility (20%, H2O): Clear, faintly yellow solution CAS # 151-21-3 Use: PAGE, blotting Quantity: 500 g Item# GR126-500

Mineral Oil
MB Grade Product Specifications Form: Clear, colorless oil Identity: by IR Specific Gravity (25 C): 0.818 to 0.880 Viscosity (40 C): 33.5 centistokes Neutrality: Passes test Readily Carbonizable Substances: Passes test Limit of Polynuclear Compounds: Passes test Solid Paraffin: Passes test Use Test: Functionally tested for use in PCR CAS # 8042-47-5 Use: IEF, 2D, PCR Quantity: 1 L Item# GR138-1

Dithiothreitol, (DTT; Cleland's Reagent)


MB Grade C4H10O2S2 Formula Weight: 154.25 Product Specifications Form: White crystalline powder Assay (SH): 99.5% A (280 nm, 0.1 M, 1 cm): 0.08 A (260 nm, 0.1 M, 1 cm): 0.40 Melting Point: 40 43 C Oxidized DTT: 0.3% DNase: Passes test RNase: Passes test Protease: Passes test CAS # 3483-12-3 Storage: -20 C Use: PAGE, 2D Quantity: 5 g Item# GR122-5

Sodium Dodecyl Sulfate (SDS), 10% Solution


Description: 10% (w/v) solution of sodium dodecyl sulfate (SDS) in high purity dH2O. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. Product Specifications A (230 nm, 3%, 1 cm): 0.2 A (280 nm, 3%, 1 cm): 0.1 Storage: Ambient Note: Precipitation of SDS is not unusual. Warm gently to redissolve. Use: PAGE, blotting Quantity:1 L Item# GR155-1

Urea
MB Grade NH2CONH2 Formula Weight: 60.06 Product Specifications Form: White crystalline powder Assay: 99.5% Identity: by IR Spectral Ratios (260 nm, 6 M, 1 cm): 0.055 Spectral Ratios (280 nm, 6 M, 1 cm): 0.044 Melting Point: 132 to 135 C Insoluble Matter: 0.01% Residue on Ignition: 0.01% Conductivity: 15 mhos/cm Cyanate: None detected Chloride (Cl): 0.0005% Sulfate (SO4): 0.001% Copper (Cu): 0.5 ppm Iron (Fe): 0.5 ppm Lead (Pb): 0.5 ppm DNase (endo): Passes test RNase: Passes test Protease: Passes test CAS # 57-13-6 Use: IEF, 2D Quantity: 1 kg Item# GR143-1

CHAPS
(3-[(3-Cholamidopropyl)-Dimethylammonio]1-Propane Sulfonate) C32H58N2O7S Formula Weight: 614.88 Product Specifications Form: White crystalline powder Identity: by IR Assay (N analysis): 98% Chromatography (TLC): Homogeneous Critical Micelle Concentration (CMC): 4 to 8 mM CAS # 75621-03-3 Storage: 4 C, protected from light Use: PAGE, IEF, 2D Quantity: 10 g Item# GR121-10

Thiourea (Thiocarbamide)
NH2CSNH2 Formula Weight: 76.12 Product Specifications Form: White to off-white crystalline powder Identity: by IR Melting Point: 171 184 C Loss on Drying: 0.5% CAS # 62-56-6 Use: IEF, 2D Quantity: 500 g Item# GR130-500

Electrophoresis Reagents

40

ELECTROPHORESIS REAGENTS

Buffer and Buffer Preparation Reagents


Tris-Glycine Buffer, 10X Solution
MB Grade Description: 10X Tris-Glycine Buffer consisting of 0.25 M Tris and 1.92 M glycine in high purity dH20, pH 8.3. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. For blotting dilute to 1X with high purity water and methanol for a solution consisting of 25 mM Tris, pH 8.3, 192 mM glycine, 20% methanol. pH (25 C, 10X): 8.3 0.1 Contaminant Testing: Protease: Passes test. Storage: Ambient Use: PAGE, blotting Quantity: 1 L Item# GR148-1

Glycine
H2NCH2CO2H Formula Weight: 75.07 Product Specifications Form: White crystalline powder Assay: 98.5 to 101.5% (dry basis) Identity: by IR Loss on Drying: 0.2% Residue on Ignition: 0.1% Sulfate (SO4): 65 ppm Heavy Metals (Pb): 20 ppm Chloride (Cl): 70 ppm Hydrolyzable Substances: Passes test O.V.I.: Passes test CAS # 56-40-6 Use: PAGE, blotting Quantity: 1 kg Item# GR125-1

PBS, 10X Solution, pH 7.4


MB Grade Description: 10X PBS (PhosphateBuffered Saline) solution consisting of 80 mM sodium phosphate, 15 mM potassium phosphate, 27 mM KCl and 1.37 M NaCl in high purity dH2O, pH 7.4. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity dH2O for a solution consisting of 9.5 mM phosphate, pH 7.4, 2.7 mM potassium chloride and 137 mM NaCl. pH (25 C, 10X): 7.4 0.1 Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test Storage: Ambient Use: Sample prep, immunodetection, cell culture, purification Quantity: 1 L Item# GR145-1

TBE Buffer, 10X ReadyMixed Powder


MB Grade Description: Pre-weighed, pre-mixed powdered buffer packaged in 200 ml bottles for reconstitution to a 10X solution. Reconstitute with high purity dH2O to 200 ml. After reconstitution, the 10X solution consists of 0.89 M Tris, 0.89 M boric acid and 0.02 M EDTA. Each pack contains 6 x 200 ml bottles. Total volume is 1.2 liters of 10X buffer or 12 liters of 1X buffer. Testing: Meets or exceeds functional test criteria for high quality gel electrophoresis. Storage: Ambient. The reconstituted buffer is stable for several days at 4 C if 0.2 m filtered. Use: DNA/RNA detection Quantity: 6 x 200 ml after reconstitution Item# GR136-6X200

Tris-Glycine-SDS Buffer, 10X Solution


MB Grade Description: 10X Tris-Glycine-SDS Buffer, consisting of 0.25 M Tris, 1.92 M glycine and 1.0% SDS in high purity dH2O, pH 8.3. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. For PAGE, dilute to 1X with high purity dH2O for a solution consisting of 25 mM Tris, pH 8.3, 192 mM glycine and 0.1% SDS. pH (25 C, 10X): 8.3 0.1 Contaminant Testing: Protease: Passes test. Storage: Ambient Application: TGS is the most commonly used buffer for SDS-PAGE (sodium dodecyl sulfatepolyacrylamide gel electrophoresis) of proteins. Use: PAGE, blotting Quantity: 1 L Item# GR149-1

Tricine
[N-Tris (Hydroxymethyl)methylglycine] (CH2OH)3CNHCH2COOH Formula Weight: 179.17 Product Specifications Form: White/clear crystalline powder Assay: 98.5% (dry basis) Identity: by IR pKa (20 C): 8.15 0.15 A (250 nm, 5%, 1 cm): 0.1 Loss On Drying: 0.5% Iron (Fe): 30 ppm Lead (Pb): 10 ppm CAS # 5704-04-1 Use: PAGE Quantity: 1 kg Item# GR131-1

TBE Buffer, 5X Solution


MB Grade Description: 5X TBE (Tris-BorateEDTA) solution consisting of 0.445 M Tris, 0.445 M boric acid and 0.01 M EDTA. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity water for a solution consisting of 0.089 M Tris, 0.089 M boric acid and 0.002 M EDTA. Testing: Meets or exceeds functional test criteria for high quality sequencing gel electrophoresis. Storage: Ambient Use: DNA/RNA detection, Sequencing Quantity: 1 L Item# GR146-1

Boric Acid
MB Grade H3BO3 Formula Weight: 61.83 Product Specifications Form: White crystalline powder. Assay: 99.5% Insoluble in MeOH: 0.005% Nonvolatile with MeOH: 0.05% Sulfate (SO4): 0.01% Heavy Metals (Pb): 0.001% Arsenic (As): 1 ppm Calcium (Ca): 0.005% Chloride (Cl): 0.001% Iron (Fe): 0.001% Phosphate (PO4): 0.001% Functional Test: Meets or exceeds criteria for high quality sequencing gel electrophoresis with Sequenase T7 DNA Polymerase. DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test CAS # 10043-35-3 Use: DNA/RNA detection Quantity: 1 kg Item# GR153-1

TE Buffer, 50X Solution


MB Grade Description: 50X TE solution consisting of 500 mM Tris, 50 mM EDTA in high purity dH2O, adjusted to pH 7.5. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity dH2O for a solution consisting of 10 mM Tris, pH 7.5, and 1 mM EDTA. pH (25 C, 50X): 7.5 0.1 Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test Storage: Ambient Use: DNA/RNA detection Quantity: 100 ml Item# GR144-100

Tris
[Tris(hydroxymethyl)aminomethane] Reagent Grade NH2C(CH2OH)3 Formula Weight: 121.14 Product Specifications Form: White to pale yellow crystalline powder Assay: 98.0% (dry basis) Identity: by IR pH (5%, H2O): 10.0 - 11.5 Melting Point: 160 C Loss On Drying: 1.5% CAS # 77-86-1 Use: PAGE, blotting Quantity: 1 kg Item# GR132-1

41

Electrophoresis Reagents

ELECTROPHORESIS REAGENTS

Caliber
TAE Buffer, 10X Solution
MB Grade Description: 10X TAE solution consisting of 400 mM Tris and 0.01 M EDTA in high purity dH2O adjusted to pH 8.3. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity dH2O for a solution consisting of 40 mM Tris Acetate, pH 8.3 and 1.0 mM EDTA. pH (25 C, 10X): 8.3 0.1 Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Storage: Ambient Use: DNA/RNA detection Quantity: 1 L Item# GR150-1

High Quality Electrophoresis Reagents

Gel Staining Reagents


TBS, 20X Solution, pH 7.4
MB Grade Description: 20X TBS (Tris-Buffered Saline) solution consisting of 500 mM Tris, 60 mM KCl and 2.8 M NaCl in high purity dH2O, pH 7.4. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity water for a solution consisting of 25 mM Tris, pH 7.4, 3.0 mM KCl and 140 mM NaCl. pH (25 C, 20X): 7.4 0.1 Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test Storage: Ambient Use: Western detection Quantity: 1 L Item# GR147-1

SSC, 20X Solution


MB Grade Description: 20X SSC solution consisting of 0.3 M trisodium citrate and 3.0 M sodium chloride in high purity dH2O, pH 7.0. Solution is 0.2 m filtered and dispensed into durable, square Nalgene bottles. pH (25 C, 20X): 7.0 0.1 Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Storage: Ambient Use: Blotting, hybridization Quantity: 1 L Item# GR127-1

Brilliant Blue G
(Coomassie Brilliant Blue G-250; C.I.42655) C47H48N3S2O7Na Formula Weight: 854.02 Product Specifications Form: Dark blue/purple crystalline powder Dye content: Approx. 60% Loss On Drying: 8% max: 607 1 nm max: 38 x 103 CAS # 6104-58-1 Application: Used for quantitative staining of proteins on polyacrylamide electrophoresis gels. Use: Protein stain Quantity: 25 g Item# GR134-25

Brilliant Blue R
(Coomassie Brilliant Blue R-250; C.I.42660) C45H44N3 S2O7Na Formula Weight: 825.97 Product Specifications Form: Dark purple/violet powder Identity: by IR max: 586 2 nm max: 45.0 x 103 Moisture (KF): 6.0% CAS # 6104-59-2 Application: Used for quantitative staining of proteins on polyacrylamide electrophoresis gels. Use: Protein stain Quantity: 25 g Item# GR135-25

Tween 20, Ultrapure


(Polyoxyethylene Sorbitan Monolaurate) Product Specifications Form: Yellow liquid Identity: Passes test Saponification Number: 40 50 mg KOH/g Hydroxyl Number: 96 108 mg KOH/g Acid Number: 2.2 mg KOH/g Water: 3.0% Specific Gravity: 1.1 g/ml (approx.) (25 C) Viscosity: 370 cps (approx.) (25 C) Solubility (10%, H2O): Soluble CAS # 9005-64-5 Use: Western detection Quantity: 500 ml Item# GR128-500

Water, RNase-Free, DEPC


MB Grade Description: High purity dH2O treated overnight with 0.1% Diethylpyrocarbonate. Product is 0.2 m filtered and dispensed into durable, square Nalgene bottles, then autoclaved. Contaminant Testing: RNase: Passes test DNase (endo): Passes test DNase (exo): Passes test Enzyme Inhibition: Passes test Storage: Ambient Use: DNA/RNA detection Quantity: 1 L Item# GR137-1

TBST, 20X Solution, pH 7.4


MB Grade Description: 20X TBS (Tris-Buffered Saline) solution consisting of 500 mM Tris, 60 mM KCl, 2.8 M NaCl, and 1.0% Tween 20 in high purity dH2O adjusted to pH 7.4. Solution is 0.2 m filtered and is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity water for a solution consisting of 25 mM Tris, pH 7.4, 3.0 mM KCl, 140 mM NaCl and 0.05% Tween 20. pH (25 C, 20X): 7.4 0.1 Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test Protease: Passes test Storage: Ambient Use: Western detection Quantity: 1 L Item# GR154-1

Water, RNase-Free, non-DEPC


MB Grade Description: High purity dH2O tested for contaminating RNase activity. Product is 0.2 m filtered and dispensed into durable, square Nalgene bottles. Contaminant Testing: RNase: Passes test DNase (endo): Passes test DNase (exo): Passes test Storage: Ambient Use: DNA/RNA detection Quantity: 1 L Item# GR139-1

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Electrophoresis Reagents

42

ULTima IMAGING PLATFORM

ULTima
Imaging Platform
The ULTima imaging platform is the fastest, most straightforward and readily upgradable unit available incorporating the latest advances in electronics and optics. The four ULTima models can economically handle everything from the most routine to the most demanding applications in proteomics and genomics which have until now required expensive laser scanning systems.

Full line of upgradable instruments (ULTima10si, 16si, 16si Plus and Pro): Allows you to upgrade to higher specifications as research needs change. Unique patented image positioning: Makes positioning of gels accurate and quick with a mininum of handling of fragile gels. Slant-correction: Permits the optical rotation of the sample for best straight visualization without touching the gel. True multi-fluorophore capabilities: Supports a wide range of applications and is particularly well suited for proteomics (Cy-dye imaging) Super Sensitivity: Performs fast chemiluminescent imaging. Enables faint-band fluorescent detection. Electronic 5 position emission filter changer: Allows automatic application setup for all imaging applications. Large image format of 21 x 26 cm: Captures full large-format proteomic gel images in a single pass. All acquisition parameters stored: Facilitates GLP use. Smaller footprint with door that slides up: Uses absolute minimum working space and eliminates the need to step back to open the door. The optional ULTima Optix illumination module provides high intensity excitation of your sample with visible and nearinfrared light.

How choose How to choose your ULTima ? ULT


The ULTima imaging line has four gel documentation systems plus the optional Optix illumination module. module. All models can be upgraded to higher specified versions giving you the flexibility to purchase the model that meets your needs and budget today and today upgrade it as your needs increase. All models come with ULTima AQ control and image acquisition software and ethidium bromide emission filter. filter. See pages 45 - 46. pag

43

ULTima Imaging Platform

ULTima IMAGING PLATFORM

Slant Correction Correction Slant correction is a unique feature that allows the user to turn the image optically 5 degrees. In this way the sample can be positioned so the analysis software is better able to perform lane detection.

Image Positioning Imag Positioning Unique image positioning using patented optics. Every user of an imaging system has experienced the problem that the gel is never in the right place initially. With most commercially available systems the user has to open the drawer or door to reposition the gel for the best visualization. The ULTima however is the only imaging system with patented optics for image positioning without loss of optical resolution. This also ensures that lens adjustments are not required. The image is positioned with the positioning keys without having to open the cabinet or adjust a fragile gel.

User Interface In order to be able to acquire a high quality image, full control of the optics is indispensable. The Image Control screen is always visible at the bottom of the image, providing ultimate control over the imaging process. The user has the choice of either setting the exposure time manually, or making use of the fully automatic capabilities of the ULTima. Full lens control allows optimization of a broad range of applications. Robot Ready The UV 26 transilluminator unit is fully accessible. Samples for automated 2D analyses can be loaded by robotic arm/system. Quantifiable Data The demanding ULTima user can be certain of producing high quantifiable data. The flat fielding function performs a uniform calibration for every individual image pixel. GLP Documentation of results and the integrity of data becomes much more important for drug discovery and genetic engineering. GLP (Good Laboratory Practice) can only be achieved if all image parameters and conditions are known and archived. ULTima is fully equipped for GLP validation and verification.

Templates Templates allow the user to define the settings for image acquisition, including the excitation and emission filters. The parameters are stored per specific application. Superior Dynamic Range Since every pixel within the image represents intensity data, it is important to achieve the highest possible conversion resolution. In most commercially available systems this is 8, 10 or 12 bit, representing 256, 1024 or 4096 grey levels. The ULTima 16si, ULTima 16si Plus and the ULTima Pro have real 16 bit dynamic ranges which means 65536 real different grey levels. This offers much more precise quantification but also allows for shorter exposure times for chemiluminescent images. The exposure time is normally much shorter than the exposure time needed for film with comparable image quality. Broad Range of Applications The emission filter changer accommodates a maximum of 5 filters. The filters are selected from the drop-down list or from free definable templates. As standard the ULTima is equipped with an emission filter for the detection of ethidium bromide and other fluorophores within in the range from 560 to 640 nm (e.g. SYPRO Ruby). Filters for most other fluorophores are available (for example SYBR Green II, Cy3, Cy5, FITC, etc.).

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

ULTima Imaging Platform

44

ULTima IMAGING PLATFORM

ULTima
ULTima 10si & 16si
Use ULTima 10si for basic documentation purposes. With ULTima 16si your measurements are more sensitive and allow more accurate quantification. Both models can be used for imaging ethidium bromide stained DNA agarose gels, Coomassie stained gels, SYBR Green stained gels, SYPRO Ruby stained gels, film archiving and petri dishes. General purpose gel documentation systems. Fluorescence ready. ULTima 10si has a 10 bit CCD camera (1024 gray levels) and ULTima 16si has a 16 bit CCD camera (65536 gray levels). Image positioning / Slant correction. Electronic optical zoom (6X). White light top illumination.
10si ORDERING INFORMATION
Max. Pixel Size 1312 x 1032 10 bit CCD

Imaging Systems ULTima Pro


The ULTima Pro is designed with innovative 2-stage Peltier cooling of the image sensor. Rather than attempt to cool the entire CCD chip to a very low temperature, the ULTima Pro cools only the areas of the chip where cooling is essential to achieve low noise levels at long measurement times. This substantially lengthens the lifetime of the CCD chip and the camera system. As with ULTima 16si Plus, the ULTima Pro is equipped with an additional computer-controlled focus and iris control. High sensitivity chemiluminescence imaging system. ULTima Pro has the top of the line 16 bit temperature controlled cooled CCD camera (65536 gray levels). Image positioning / Slant correction.

ULTima 16si Plus


High dynamic range general purpose gel documentation system. ULTima 16si Plus has a 16 bit CCD camera (65536 gray levels). Image positioning / Slant correction. Electronic optical iris. Electronic optical focus. Electronic optical zoom (6X). White light top illumination. ULTima 16si Plus is equipped for multi-plexed multi-fluorophore applications.
16si Plus ORDERING INFORMATION
Max. Pixel Size 1392 x 1040 16 bit CCD

Electronic optical iris. Electronic optical focus. Electronic optical zoom (6X). White light top illumination.

ULTima 10si Imaging System Item# ULT10 Includes: UV-26 Transilluminator ULTima AQ communication software suite Total Lab TL 100 analysis software ULTima 10si Imaging Unit Item# ULT101 Includes: ULTima AQ communication software suite

ULTima 16si Plus Imaging System plus FILTER SELECTOR Item# ULT16F Includes: UV-26 Transilluminator ULTima AQ communication software suite Total Lab TL 100 analysis software ULTima 16si Plus Imaging Unit plus FILTER SELECTOR Item# ULT161F Includes: ULTima AQ communication software suite

Pro ORDERING INFORMATION


Max. Pixel Size 1392 x 1040 16 bit Cooled CCD

ULTima Pro Cooled Imaging System Item# ULT16C Includes: UV-26 Transilluminator ULTima AQ communication software suite Total Lab TL 100 analysis software ULTima Pro Cooled Imaging Unit Item# ULT161C Includes: ULTima AQ communication software suite

16si ORDERING INFORMATION


Max. Pixel Size 1392 x 1040 16 bit CCD

ULTima 16si Imaging System Item# ULT16 Includes: UV-26 Transilluminator ULTima AQ communication software suite Total Lab TL 100 analysis software ULTima 16si Imaging Unit Item# ULT161 Includes: ULTima AQ communication software suite

ORDERING INFORMATION

UV-26 Transilluminator (see pg. 49) Item# UV26

45

ULTima Imaging Platform

ULTima IMAGING PLATFORM

ULTima T ECHNICAL SPECIFICATIONS

ULTima 10si
Resolution (pixel) Resolution (sec.) Image Sensor Cooling Camera Bit Depth 1312 x 1032 0.001 - 10 No 10

ULTima 16si
1392 x 1040 0.001 - 10 No 16

ULTima 16si Plus


1392 x 1040 0.001 - 10 No 16

ULTima Pro
1392 x 1040 0.001 - 3000 Yes, 6 arrays 16

Electronic Optical Zoom Electronic Iris Control Electronic Focus Control

6x No No

6x No No

6x Yes Yes

6x Yes Yes

Image Position System Slant Control

Yes 5 degrees

Yes 5 degrees

Yes 5 degrees

Yes 5 degrees

Emission Filter Positions White Light Top Illumination Automatic Door Lock

1 (5 optional) Yes No

1 (5 optional) Yes No

1 (5 optional) Yes No

1 (5 optional) Yes Yes

Contrast Control Brightness Control Flat Fielding Saturation Indication Image Addition (x) Addition Interval (min.)

Yes Yes Yes Yes No No

Yes Yes Yes Yes 1 - 25 0 - 20

Yes Yes Yes Yes 1 - 25 0 - 20

Yes Yes Yes Yes 1 - 25 0 - 20

Filetype: tiff (16 bit), Tif (8 bit), bmp, jpg, gif

Mains: 110/230 Voltage AC automatic detection 50-60 Hz 180 Watt max. Installation Cat. II

Outer Dimensions (W x H x D): 510 x 380 x 500 mm

Weight: 41 kg.

For installing and using the ULTima and ULTima AQ software the following computer minimum specifications are required: Computer working under Windows 2000, Windows XP-Home (SP1) or XP-Professional (SP1) minimum 256 Mb RAM, minimum screen resolution 1024 x 768, one free USB 2.0 port (800 mA or 500 mA) one free RS-232C port (9 pin), pointing device, 1 Gb free harddisk space.

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

ULTima Imaging Platform

46

ULTima IMAGING PLATFORM

Optix

Patented Optical Module

TotalLab

Analysis Software

The Optix module is a patented epi-illumination option for the excitation of fluorophores within the VIS/NIR range. When Optix is used for ethidium bromide stained gels, it offers the following advantages: DNA remains intact for applications downstream. Lower background means higher detection limits. Selection of excitation wavelength is fully computer controlled and, in combination with the automatic emission filter changer, true multi fluorophore capability is achieved. Multiple images with different excitation/emission filter sets allow the detection of multiple fluorophores within the same sample. The Optix can accommodate a maximum of five different excitation filter sets. The Optix module can be added to all models of the ULTima line. Applications that are possible with the addition of the Optix module include: CyDye imaging, array detection, in vivo GFP detection and GFP tissue and organ screening.

Hoefer offers a choice of software solutions for the analysis of your 1D gels and blots. TL 100 - Basic entry level software for fast, consistent analysis TL 120 - For an in-depth and accurate automatic analysis of 1D gels and band pattern matching studies Please select the software that best meets your needs by identifying your area of work below: Molecular Weight Calibration The TL 100 and TL 120 versions both enable precise molecular weight determination of band material from a variety of 1D gels. TL 120 contains tools which allow increased control over calibration for particularly distorted 1D gel images. Quantitation and Normalization of Band Material The TL 100 and TL 120 software solutions both contain a complete range of tools to quantify band material and for the calculation of normalized band volumes. For in-depth quantitative studies, the advanced features in TL 120 offer a higher degree of flexibility and facilitate accurate quantitation of bands on more problematic 1D gel images. Lane Relationship Studies The TL 120 software has a powerful band matching facility which is flexible yet easy to use. There are tools available to visualize the results of matching and identify similarities within a single gel. When you have performed intra gel matching, lanes can be classified and identified. Summary of features in the TotalLab range: TL 100 Automatic detection of lanes and bands Automatic background subtraction Lane templates TL 120

Pseudo-color image of macro array, labeled with Cy5.5

GFP detection in leaves.

Image manipulation tools Analysis protocols for batch processing Molecular weight calibration Quantity calibration Normalisation Profile deconvolution

ORDERING INFORMATION

Rf calibration Band picking Band pattern matching - single gel Dendrogram - single gel Reports Array, dot / slot blot analysis Colony counting A toolbox for general image analysis
ORDERING INFORMATION

Optix Illumination Module Item# ULT164

TotalLab TL 100 Analysis Software TotalLab TL 120 Analysis Software Single user license. Single user license. Item# ULT162 Item# ULT163

47

ULTima Imaging Platform

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

ULTima IMAGING PLATFORM

Analysis protocols enable consistent processing of multiple gels in TL 120.

TL 100 Control Center

ID Gel Image analyzed by TL 100

Calibration of a 1D gel using multiple molecular marker lanes in TL 120.

Rf calibration accounts for distortions across the gel in TL 120.

ULTima Imaging Platform

48

MacroVue UV-20 & UV-26


UV Transilluminators
HOEFER MACROVUE UV TRANSILLUMINATORS are available in two sizes. The active area of the UV-20 is 20 x 20 cm; the active area of the UV-26 is 21 x 26 cm. Uniform illumination. High (9000 W/cm2) or low intensity operation (7000 W/cm2) for analytical or preparative applications. Shortwave UV lamps are available to change illumination from 302 nm to 254 nm. UV safety cover minimizes personal exposure.
ORDERING INFORMATION T ECHNICAL SPECIFICATIONS

IMAGING

MacroVue UV-26, Universal Voltage Item# UV26 MacroVue UV-20, 115 VAC Item# UV20-115V MacroVue UV-20, 230 VAC Item# UV20-230V

Illuminated Area: UV-26: 21 x 26 cm UV-20: 20 x 20 cm Ultraviolet Lamps: 8 W 302 nm UV dual bi-pin discharge type tubes. UV-26: Six lamps UV-20: Five lamps UV-20 intensities, approx.: high: 9,000 W/cm2 low: 7,000 W/cm2 Unit Dimensions (W x H x D): UV-26: 35.5 x 12.1 x 35 cm (14" x 4.75" x 13.75") UV-20: 33.7 x 12.1 x 24.1 cm (13.25" x 4.75" x 9.5") Safety Certifications EN61010-1, UL 3101-1, CSA22.2 1010-1, CE

Each Model Includes: UV Transilluminator w/ Intensity Switch UV Lamps, 302 nm (installed) 5 lamps (UV-20) or 6 lamps (UV-26) Hinged Clear UV-Blocking Safety Cover

MacroVue UVis-20
The MACROVUE UVIS-20 UV + VISIBLE LIGHT TRANSILLUMINATOR houses both 302 nm midrange UV and visible (white) light lamps. Each filter is 20 x 20 cm. Versatile. Just push a button to change from one type of illumination to the other. Illuminates uniformly. Dual intensity UV lamps and long-life filters accommodate analytical and preparative applications. Shortwave UV lamps are available to change illumination from 302 nm to 254 nm. UV safety cover minimizes personal exposure.

Combination UV + Visible Light Transilluminator

ORDERING INFORMATION

SPECIFICATIONS

MacroVue UVis-20, 115 VAC Item# UVIS-20-115V MacroVue UVis-20, 230 VAC Item# UVIS-20-230V

Illuminated Area: two 20 x 20 cm surfaces Ultraviolet Lamps: UV: Five 8 W 302 nm UV White light: Two 8 W visible light UV intensity, approx.: 8,000 W/cm2 Unit Dimensions (W x H x D): 48.6 x 14.3 x 33.7 cm (19.1" x 5.6" x 13.3") Safety Certifications EN61010-1, UL 3101-1, CSA22.2 1010-1, CE

Each Model Includes: Combination UV + White Light Transilluminator UV Lamps, 302 nm 5 Lamps (installed) Cool White Lamps 2 Lamps (installed) Hinged Clear UV-Blocking Safety Cover

MacroVue Accessories & Spare Parts


UV Lamp, 8 W, 302 nm Item# UVLM-8 UV Lamp, 8 W, 254 nm Item# UVLS-8 Cool White Lamp, 8 W Inquire

49

Imaging

Hinged UV-Blocking Safety Cover (for UV-20 or UV-26) Item# UV20RK-1

Hinged UV-Blocking Safety Cover (for UVIS-20) Inquire

QUANTITATION

T ECHNICAL SPECIFICATIONS

ORDERING INFORMATION

DQ 300 Fluorometer
DNA/RNA Quantitation
The DQ 300 FLUOROMETER is a compact, dual-channel unit designed to produce quick, easy, and accurate fluorescence measurements. Choose either ultraviolet or blue excitation wavelengths. Detects DNA accurately in the presence of interfering RNA or protein. Detects down to 1 ng of DNA or RNA in 1 ml. Assay low volume samples with the optional Minicell adapter. Operates in two modes: Discrete (one time 5-second average) or Continuous (user-defined serial measurements). Logs up to 1000 data points in internal memory. RS-232 cable connects to the serial port of a PC and outputs results using spreadsheet interface software supplied.

Excitation Range: UV Channel: 365 - 395 nm Blue Light Channel: 465 - 485 nm Emission Range: UV Channel: 440 - 470 nm Blue Light Channel: 515 - 575 nm Sensitivity: 1 ng/mL DNA using PicoGreen 1 ng/mL RNA using RiboGreen 10 ng/mL DNA using Hoechst 33258 100 ng/mL protein using NanoOrange Unit Dimensions (W x H x D): 14 x 6.9 x 18.4 cm (5.5" x 2.7" x 7.25") Safety Certifications: EN61010-1, UL 3101-1, CSA22.2 1010-1, CE

DQ 300 Fluorometer Item# DQ300 Includes: Fluorometer Power Supply Kit Spreadsheet Interface Software CD-ROM Methacrylate Cuvettes - 4 pcs RS-232 Serial Cable Minicell Adapter Kit (for 50 - 250 l samples) Item# DQ310 Includes: Minicell Adapter Borosilicate Glass Tube Cuvettes - 400 pcs

Accessories & Spare Parts


Standard Polystyrene Cuvettes - 100 pcs, (10 x 10 mm square, 3.5 ml), Item# DQ305 Standard Methacrylate Cuvettes - 100 pcs, (10 x 10 mm square, 3.5 ml), Item# DQ306 Minicell Borosilicate Glass Tube Cuvettes - 400 pcs, (cylindrical, 250 l), Item# DQ315 Hoechst 33258 Dye 100 mg, Item# DQ201 Calf Thymus DNA, Fluorescence Standard 250 g, Item# DQ202 4-Methylumbelliferone, Item# DQ203

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Quantitation

50

DETECTION

HB-1000
Stackable design to maximize laboratory space. Temperature control: ambient +10 C to 99.9 C.

Hybridization Oven

UVC 5000

Ultraviolet Crosslinker

The HB-1000 HYBRIDIZATION OVEN is designed to create the appropriate environment for nucleic acid hybridization.

The new UVC 5000 UV CROSSLINKER features a pull-out drawer and microprocessor controls to ensure optimal UV irradiation of DNA. It is also suitable for UV bonding and photonicking of DNA. The crosslinker can also be stacked on top of the HB-1000 Hybridization oven to maximize laboratory space. Choice of preset and manual controls for energy or time-limit exposures. Preset limits deliver 120,000 J or five min of exposure. Large LED display and touch keypad. UV-blocking window allows safe viewing of the process. Microprocessor measures and controls UV output, ensuring maximum dose consistency.

Variable speed control (10 to 18 RPM) for consistent wetting of samples, for washing or hybridizing. A large LED displays current chamber temperature. An adjacent touch pad sets the temperature. The rotary tube rack can be replaced with a rocker plate for dish-style incubation and washing protocols in one benchtop unit. A removable protective tray allows easy spill cleanup. The rotary wheel can hold twenty 35 x 150 mm, or ten 35 x 300 mm tubes at one time. Stainless steel internal construction. Multiple tube sizes available.

51

Detection

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

UVC 5000 T ECHNICAL SPECIFICATIONS

UV Lamps: Five 8 W 254 nm UV dual bi-pin discharge type tubes. Maximum Settings: Energy: 999,900 J/cm2 Time: 999.9 min Features: Microprocessor controlled UV sensor feedback system, large LED readout, multiple set functions (pre-set or user-specified UV energy or time) Unit Dimensions (W x H x D): external: 40.0 x 22.2 x 34.9 cm (19.1" x 5.6" x 13.25") chamber: 25.4 x 12.7 x 30.5 cm (10.0" x 5.0" x 12.0") Safety Certifications: EN61010-1, UL 3101-1, CSA22.2 1010-1, CE

DETECTION

Hybridization Oven & Ultraviolet Crosslinker stack to maximize lab space!

UVC 5000 ORDERING INFORMATION

UVC 5000 Ultraviolet Crosslinker, 115 VAC Item# UVC5000-115V UVC 5000 Ultraviolet Crosslinker, 230 VAC Item# UVC5000-230V Each Model Includes: UV Crosslinker UV Lamps, 254 nm 5 Lamps

Accessories & Spare Parts


UV Lamp, 8 W, 254 nm Item# UVLS-8
HB-1000 T ECHNICAL SPECIFICATIONS

Variable Speed: 10 to 18 RPM Heating Element: 1200 Watts Operating Range: Ambient to +10 C to 99.9 C Temperature Control Accuracy: +0.1 C Temperature Display: LED Temperature Stability: Inside Oven: +0.3 C to 68 C Inside Bottles: +0.1 C to 68 C Temperature Uniformity: Inside Oven: +0.1 C to 68 C Inside Bottles: +0.1 C to 68 C Bottle Capacity: Twenty (20) 35 x 150 mm Ten (10) 35 x 300 mm Power: 12V AC/DC from 115V/60Hz or 230V/50Hz Safety Certification: CE

HB-1000 ORDERING INFORMATION

HB-1000, Hybridization Oven, 115 VAC Item# HB1000-115V HB-1000, Hybridization Oven, 230 VAC Item# HB1000-230V

Accessories & Spare Parts


Bottle, 35 x 150mm, Item# HB1110X Bottle, 35 x 300mm, Item# HB1111X Rocker plate, Item# HB1140X
Detection

52

DETECTION

ORDERING INFORMATION

SE 1200 Easy Breeze, 115 VAC Item# SE1200-115V SE 1200 Easy Breeze, 230 VAC Item# SE1200-230V Includes: Easy Breeze Drying Oven Standard Gel-Drying Frame Assemblies 2 sets Loading Platform Porous Cellophane Sheets 50 pcs Mylar Sheets 25 pcs

SE 1200 Easy Breeze

T ECHNICAL SPECIFICATIONS

Operating Conditions: Environmental Operating Conditions: Indoor Use: 4 - 40 C Humidity: up to 80% Altitude: up to 2000 m Unit Dimensions (W x H x D): 32 x 27 x 51 cm (12.5" x 10.5" x 20") Safety Certifications: EN61010-1, UL 3101-1, CSA22.2 1010-1, CE

Air Gel-Drying System

Hoefers compact SE 1200 EASY BREEZE AIR GEL-DRYING SYSTEM comes complete with two gel frames -- everything you need to dry two gels as large as 20 x 20 cm, or eight 8 x 10 cm gels. Air circulates above and below the gel frames producing even heating that dries gels without cracks. Drying oven accommodates 6 Hoefer gel frames, allowing you to dry as many as 6 standard or 24 minigels at a time. Gel frames are easy to assemble and load. Gels are held taut and smooth. No wrinkling. Temperature adjusts from 40 to approximately 50 C. You can also dry gels without heat using just the fan. No need to monitor the unit. Most gels dry in 2 to 3 hours. Requires no vacuum pump, cold trap, or bulky connections.

Accessories & Spare Parts


Gel-Drying Frame Assemblies: Standard (up to 20 x 20 cm gel) Item# SE1210 Large (up to 21 x 26 cm gel) Item# SE1210-L Gel-Loading Platform: Standard (for SE 1210 gel frame) Item# SE1214 Large (for SE 1210-L gel frame) Item# SE1214-L Porous Cellophane: Standard, 33 x 33 cm (50 pcs) Item# SE1202 Large, 35 x 44 cm (50 pcs) Item# SE1142 Mylar: Standard, 33 x 33 cm (25 pcs) Item# SE1204

53

Detection

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

The Hoefer GD 2000 VACUUM GEL-DRYING SYSTEM uses a dry-heat vacuum method to dry gels rapidly, evenly, and safely.

DETECTION

GD 2000

Dries acrylamide and agarose gels as large as 33 x 44 cm in as little as 30 minutes. Heat and vacuum are applied from beneath the gel through a heavy aluminum platen for even heat and vacuum distribution under the gel. Independent timers for heat and for vacuum. Thermostat adjusts from 40 to 80 C, 2 C. A quiet, oil-free diaphragm pump maintains the low vacuum necessary for rapid gel drying.

Vacuum Gel-Drying System

T ECHNICAL SPECIFICATIONS ORDERING INFORMATION

Accessories & Spare Parts


Filter Paper, 35 x 44 cm (25 sheets) Item# SE1141 Porous Cellophane, 35 x 44 cm (50 sheets) Item# SE1142 Clear Silicone Rubber Overlay Sheet Item# SE1143 Mylar Cover Sheet Item# SE1144 Porous Polyethylene Cover Sheet Item# SE1145 Stainless Steel Screen Item# SE1146 Vacuum Tubing, 8 mm ID, 3 m Item# VT3

GD 2000 Vacuum Gel-Drying System, 115 VAC Item# GD2000-115V GD 2000 Vacuum Gel-Drying System, 230 VAC Item# GD2000-230V Includes: GD 2000 Gel Dryer w/Silicone Rubber Overlay Sheet VP 200 Vacuum Pump Stainless Steel Screen Porous Polyethylene Cover Sheet Mylar Cover Sheet Porous Cellophane 50 sheets Filter Paper 10 sheets Vacuum Tubing 3 m

Maximum Operating Ranges: Heater: 800 W, thermostatically controlled Pump Outlet: 575 W Power Requirements: 115 VAC, 50/60 Hz, or 230 VAC, 50/60 Hz Operating Conditions: Environmental Operating Conditions: Indoor Use: 15 - 40 C Humidity: up to 80% for 15-31 C, decreasing linearly to 50% for 31-40 C Altitude: up to 2000 m Unit Dimensions (W x H x D): 55.0 x 8.5 x 43.5 cm (21.6" x 3.4" x 17") Safety Certifications: EN61010-1, UL 3101-1, CSA22.2 1010-1, CE

Detection

54

RADIATION SAFETY

Radiation Safety
Hoefer Beta and Gamma Radiation Protection Devices
Protect your lab team from low-level radioactive emissions with Hoefer beta and gamma shields, storage boxes, cabinet workstations, and waste receptacles. All products offer effective protection and an unobstructed view. Shields, boxes, and bins are available for either beta or gamma radiation protection. , 10 mm thick optical acrylic products block beta-emitting isotopes such as 32P
123 125 133 35

S, 3H, and 14C.

12 mm thick lead-acrylic products protect from gamma-emitting isotopes such as I, I, Xe, 57Co, 99mTe, and 67Ga.
Beta Adjust. Shield

SHIELDS Hourglass shields provide easy access to the central area behind the shield. Rectangular shields have adjustable (0 to 15) or fixed angles (15 for working in a sitting position or 45 for standing).
Beta Storage Box, Mini

STORAGE, TRANSPORT, AND DISPOSAL BOXES Storage boxes come in three sizes: Mini, Midi, and Maxi. Tube-holding inserts are available for each size. Easy to use pipette tip disposal boxes minimize exposure.

Storage Box Insert, Mini

BETA RADIATION PROTECTION CABINET Offers all-around protection for operators and those working nearby. Affords easy access to work area through two large hinged doors. Users have an unobstructed view whether sitting or standing.
Beta Storage Box, Mini

HINGED-LID WASTE BINS AND BAGS Bins safely hold low-level radioisotope waste. Heavy-duty plastic waste bags fit conveniently inside all bins and close with double-string neck pull. Waste bins and bags are available in a variety of sizes.
Cabinet Workstation Storage Box Insert, Mini

GEL CASTING KITS SEE PAGE 24

Gamma Shield

Waste Bin, 10 L

Storage Box Insert, Mini

Beta Storage Box, Maxi

55 1.1 Radiation Safety

Hoefer Radiation Safety Products Ordering Information


BETA-BLOCKING PRODUCTS
All products indicated Beta below are made from 10 mm thick optical acrylic and block Beta-emitting isotopes such as 32P 35S, 3H, and 14C. ,
Item Number RPP-S015 RPP-S15SC RPP-S15LC RPP-S45SC RPP-SHC RPP-B5 RPP-B6 RPP-B14 RPP-BI-16 RPP-BI-32 RPP-BI-40 RPP-BI-8 RPP-BI-9 RPP-BI-1 RPP-BI-2 RPP-FB RPP-STB RPP-B15SC RPP-C RPP-B12 RPP-B17 RPP-B27 RPP-B31 RPP-B40 RPP-BAG17 RPP-BAG31 Type of Blocking Beta Beta Beta Beta Beta Beta Beta Beta Product Shield, adjustable Shield, fixed 15 angle Shield, fixed 15 angle Shield, fixed 45 angle Shield, hourglass Storage box, Mini Storage box, Midi Storage box, Maxi Storage box insert, Mini Storage box insert, Midi Storage box insert, Midi Storage box insert, Midi Storage box insert, Midi Storage box insert, Maxi Storage box insert, Maxi Flipper box Storage and transport box Tip disposal box Cabinet workstation Waste bin, 1L Waste Bin, 3.3L Waste Bin, 10L Waste Bin, 15L Waste Bin, 20L Waste Bag Waste Bag Description Beta rectangular shield, 540 x 350mm, with adjustable angle 0 to 15 Beta rectangular shield, small (450x 300 mm), with curved base and fixed 15 angle Beta rectangular shield, large (530x 350 mm), with curved base and fixed 15 angle Beta rectangular shield, small (450x 300 mm), with curved base and fixed 45 angle Beta hourglass shield, 450 x 300mm, with curved base Mini Beta storage box with hinged lid, 55x85 x85mm (HxWxD) Midi Beta storage box with hinged lid, 60x165x85mmID (HxWxD) Maxi Beta storage box with hinged lid, 140x280x165mmID, (HxWxD) Mini storage box insert for 16x1.5 ml tubes Midi storage box insert for 32x1.5 ml tubes Midi storage box insert for 40x0.5 ml tubes Midi storage box insert for 16x1.5ml and 16x0.5ml tubes Midi storage box insert for 32x2ml Cryotubes Maxi storage box insert for 15x15ml centrifuge tubes Maxi storage box insert for 8x50ml centrifuge tubes Beta flipper box for 4-Way FlipperTM racks, 140x 175x150mmID Beta storage and transport box, 70x150x120mmID, specially machined to accept 24x1.5ml Eppendorf tubes Beta pipette tip disposal box. Uses RPP-BAG17 waste bags. Beta cabinet workstation with hinged doors, 490 x 550 x 370 mm ID Beta waste bin, 1 L capacity Beta waste bin, 3.3 L capacity. Uses RPP-BAG17 waste bags. Beta waste bin, 10 L capacity. Uses RPP-BAG31 waste bags. Beta waste bin, 15 L capacity. Uses RPP-BAG31 waste bags. Beta waste bin, 20 L capacity. Uses RPP-BAG40 waste bags. Heavy duty polyethylene waste bags with double string neck pull, 120x 120 x 120 mm, for RPP-B17 bin, pk/25 Heavy duty polyethylene waste bags with double string neck pull, 290x 210 x 210 mm, for RPP-B27, RPPGB27, and RPP-B31 bins, pk/25 Heavy duty polyethylene waste bags with double string neck pull, 370x 210 x 240 mm, for RPP-B40 bin, pk/25 Radiation hazard symbol adhesive labels, 50x 50 mm, pk/25

RADIATION SAFETY

Beta Beta Beta Beta Beta Beta Beta Beta Beta

RPP-BAG40 RPP-LAB50

Waste Bag Radiation hazard labels

GAMMA-BLOCKING PRODUCTS
All products indicated Gamma below are made from 12 mm thick lead-acrylic and block Gamma-emitting isotopes such as 123I, 125I, 133Xe, 57Co, 99mTe, and 67Ga.
Item Number RPP-GS015 RPP-GS15SC RPP-GS15LC RPP-GS45SC RPP-GSHC RPP-GB6 RPP-BI-32 RPP-BI-40 RPP-BI-8 RPP-BI-9 RPP-GB15SC RPP-GB27 RPP-BAG31 RPP-LAB50 Type of Blocking Product Gamma Gamma Gamma Gamma Gamma Gamma Shield, adjustable Shield, fixed 15 angle Shield, fixed 15 angle Shield, fixed 45 angle Shield, hourglass Storage box, Midi Storage box insert, Midi Storage box insert, Midi Storage box insert, Midi Storage box insert, Midi Tip disposal box Waste bin, 10L Waste bag Radiation hazard labels Description Gamma rectangular shield, 540 x 350 mm, with adjustable angle 0 to 15 Gamma rectangular shield, small (450x 300 mm), with curved base and fixed 15 angle Gamma rectangular shield, large (530x 350 mm), with curved base and fixed 15 angle Gamma rectangular shield, small (450x 300 mm), with curved base and fixed 45 angle Gamma hourglass shield, 450x300mm, with curved base Midi Gamma storage box with hinged lid, 60x165x 85mmID (HxWxD) Midi storage box insert for 32x1.5 ml tubes Midi storage box insert for 40x0.5 ml tubes Midi storage box insert for 16x1.5ml and 16x0.5ml tubes Midi storage box insert for 32x2ml Cryotubes Gamma pipette tip disposal box. Uses RPP-BAG17 Waste Bags. Gamma hinged-lid waste bin, 10 L capacity. Uses RPP-BAG31 waste bags. Heavy duty polyethylene waste bags with double string neck pull, 290x210x210 mm, for RPP-B27, RPPGB27, and RPP-B31 bins, pk/25 Radiation hazard symbol adhesive labels, 50x 50 mm, pk/25

Gamma Gamma

*Safety Precaution: 12 mm thick lead-acrylic is suitable only for protection from gamma emissions from 125I and not from higher isotopes of iodine. Danger: Do not use lead or lead-acrylic for shielding beta emissions.

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Radiation Safety

56

AMINO ACID ANALYZER

Biochrom 30 Series

Amino Acid Analyzer

The Biochrom 30 Series Amino Acid Analyzer is a sophisticated PC controlled liquid ion exchange chromatography system dedicated to providing rapid, specific amino acid analysis for clinical, pharmaceutical, proteomics, food and feedstuff industries.

Biochrom 30 Amino Acid Analyzer The Biochrom 30 Amino Acid Analyzers comprise instrument, 84 position cooled autosampler, analytical column with top-up resin, prewash column, operating software, EZchrom Elite data handling software, chemical kit with calibration standard, Ninhydrin and buffer bottles, instruction manuals, tool kit and spare parts/consumables kit. The system also includes Pentium PC and Deskjet printer. The systems offer: Convenience: Load and Go post-column photometric detection of amino acids with no additional derivatization procedures. Flexibility: Integrated software enables automatic sample set-up, flexible samples analysis, networking and exporting of data. Versatility: A wide variety of sample types can be analyzed using the available range of programs and columns. Serviceability: Ergonomic design for ease of maintenance. Biochrom 30 Physiological Designed for metabolic disorder screening and research, a full routine analysis of physiological fluids can be performed enabling the determination of GEL CASTING KITS up to 53 amino acids.
SEE PAGE 24

Biochrom 31 Protein Designed for quality control in drug synthesis and process control and authenticity testing, the system can rapidly deal with complex samples and high salt concentrates.

Biochrom 32 Oxidized Proteins Designed for the analysis of complex hydrolysates such as food and feedstuffs, the system meets the requirements of the standard methods from the AOAC and the EU Commission Directive 98/64/EC.

59 1.1 Bio 30

AMINO ACID ANALYZER

Biochrom 30 Series Ordering Information


SYSTEMS REAGENTS

Biochrom 30 Series Amino Acid Analyzers Physiological Instrument Item# 80-2111-00 Biochrom 30 Physiological System Includes: Pentium PC Deskjet printer EZchrom Elite handling software Item# 80-2111-00-SYS Protein Hydrolysate Instrument Item# 80-2111-01 Biochrom 31 Protein Hydrolysate System Includes: Pentium PC Deskjet printer EZchrom Elite handling software Item# 80-2111-01-SYS Oxidized Hydrolysate Instrument Item# 80-2111-02 Biochrom 32 Oxidized Hydrolysate System Includes: Pentium PC Deskjet printer EZchrom Elite handling software Item# 80-2111-02-SYS

Sodium citrate buffer pH 2.2, 2 L Item# 80-2037-67 Sodium citrate buffer pH 2.65, 2 L Item# 80-2037-79 Sodium citrate buffer pH 3.2, 2 L Item# 80-2037-68 Sodium citrate buffer pH 3.35, 2 L Item# 80-2037-80 Sodium citrate buffer pH 4.25, 2 L Item# 80-2038-08 Sodium citrate buffer pH 6.45, 2 L Item# 80-2038-09 Borate/citrate buffer pH 8.6, 2 L Item# 80-2037-88 Lithium citrate buffer pH 2.2, 2 L Item# 80-2038-10 Lithium citrate buffer pH 3.55, 2 L Item# 80-2037-69 Lithium citrate buffer A, 2 L Item# 80-2038-15 Lithium citrate buffer B, 2 L Item# 80-2038-16 Lithium citrate buffer C, 2 L Item# 80-2038-17 Lithium citrate buffer CII, 4151-II Item# 80-2099-83 Lithium citrate buffer D, 2 L Item# 80-2038-18 Lithium citrate buffer D II, 2 L Item# 80-2097-18

Calibration standard physiological fluid Item# 80-2037-62 Sodium hydroxide solution, 1 L Item# 80-2037-57 Lithium hydroxide solution, 1 L Item# 80-2038-20 Ultra Clean solvent Item# 80-2117-75 Ultra Ninhydrin Reagent Kit 2 L 80-2118-30 Ultra Ninhydrin Reagent Kit 8 L 80-2117-76 Ultra Physiological Fluid Chemical Kit. Sufficient for up to 500 hours of continuous operation. 80-2117-77 Ultra Protein Hydrolysate Chemical Kit. Sufficient for up to 320 hours of continuous operation. 80-2117-78 Ultra Oxidized Feedstuff Chemical Kit. Sufficient for up to 360 hours of continuous operation. 80-2117-79

Hoefer is the exclusive distributor for the Biochrom 30 series of Amino Acid Analyzers in the United States. of For information on distributors and support in other countries please visit the Biochrom Website at: www.biochrom.co.uk please

800.227.4750 (USA)

1.415.282.2307 (INT.)

FAX 1.415.920.4494

Bio 30

60

HARVARD APPARATUS

HARVARD APPARATUS
Equilibrium Dialysis For Binding Studies
Single sample or patented 96-well format 5K and 10K MWCO Re-usable PTFE or disposable chambers Easy-to-use SBS compatible for automation

Complete Solutions for your Sample Preparation Requirements: dialysis, electrodialysis, equilibrium dialysis; filtration; Micro SPE.

Economical Filtration Plates


48, 96 and 384 well formats Many designs for different sample sizes Membranes from 0.45-25m (others available by request) Large selection of pore sizes, materials and shapes

2D ElectroPrep Sample Fractionation Device


The New ElectroPrep System is an extremely versatile patented sample prep technology based on (2D) electrophoresis/dialysis. The system can, in three minutes, dialyze a sample simultaneously into multiple MWCO fractions. The system is also ideal for iso-electric focusing of protein samples and can be used to fractionate samples before 2D electrophoresis. ElectroPrep has a broad range of membranes that it supports. Membranes with MWCO (molecular weight cut off) of:100, 500, 1K, 2K, 5K, 10K, 25K, 50K and 100K are available. Ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules. With a run-time of 3 to 10 minutes, ElectroPrep provides speed and convenience, even at the very low currents (5 to10 mA) used with this system. The sample chambers are made of PTFE which is a completely inert material especially suited for high sample recovery. Membranes of different MWCO are available from 100 to 100,000 MWCO and can be used for selective elution, filtration, dialysis, fractionation and concentration. A fast DIALYZER can be joined with up to 7 multiple link chambers in different combinations and membranes.

61

Harvard Apparatus

Breakthrough In Sample Preparation


Patented C-18 PrepTipTM for manual or automated sample prep No Backpressure makes PrepTipTM easy-to-use High precision and accuracy of sample binding 3 l bed volume, 10 l tip volume Ideal for de-salting, buffer exchange, sample enrichment

HARVARD APPARATUS

CoZap The Quick and Easy Destainer


CoZap is used for rapid removal of Coomassie Blue stain from electrophoresis gels without the need to change the destaining solution. CoZap is a unique pad that has high absorbance for Coomassie Blue and is thus very effective in destaining gels. CoZap absorbs any free dye in the solution making gel destaining 20% faster than conventional methods. No need to change the destaining solution Fast and Simple No charcoal or dye residues No subsequent destaining required One pad can destain up to 10 gels 20% faster than conventional method

High Performance Spincolumns


Single or 96 well formats Unrivaled selection of column chemistries: G10, 25, 50,100; P2, 6; C18,8, 4; CN, NH2, PVOH and PHEA Strong & weak anion and cation, charcoal, cellulose and IMAC High sample recovery rates Economically priced Spincolumns varied sample sizes: 10-25 l, 25-75 l, 75-150 l Ideal for de-salting, buffer exchange and sample enrichment

For application assistance or a free catalog call Harvard Apparatus at 800 -272-2775 or register at www.harvardapparatus.com or email us at bioscience@harvardapparatus.com.

Harvard Apparatus

62

TECHNICAL APPENDIX

Technical Appendix
Protein Electrophoresis
Recommended acrylamide concentrations for protein separation
Separation size range (kD) 36 - 205 24 - 205 14 - 205 14 - 66* 14 - 45* % Acrylamide in resolving gel 5% 7.5% 10% 12.5% 15%

Protein marker characteristics


Protein - 2 macroglobulin - lactalbumin actin albumin amyloglucosidase aprotinin - galactosidase - lactoglobulin A carbonic anhydrase carbonic anhydrase catalase conalbumin creatine phosphokinase cytochrome c ferritin GAPDH glucose oxidase glutamate dehydrogenase insulin A chain insulin B chain lactate dehydrogenase lentil lectin lysozyme myoglobin myosin ovalbumin pepsinogen phosphorylase b thyroglobulin transferrin triose phosphate isomerase trypsin inhibitor trypsinogen Source bovine plasma bovine milk bovine muscle bovine serum Aspergillus niger bovine pancreas Escherichia coli bovine milk bovine erythrocyte human bovine liver egg white rabbit muscle equine heart horse spleen rabbit muscle Aspergillus niger bovine liver bovine bovine beef heart lentil egg white equine heart rabbit muscle egg white porcine stomach rabbit muscle hog thyroid human rabbit soybean bovine pancreas Use* S S S N, S I S S I S, I I N I I I N I I S S S N I S S, I S S I S N S S S, I I Native Mr (x103) 170 14.4 43 66 6.5 116 35 30 232 81 12.4 440 140 160 53 2.5 3.5 140 Subunit Mr (x103) 170 14.4 43 66 6.5 116 18.4 30 35 60 76 40 12.4 220, 18.5 36 53 2.5 3.5 36 50 14.4 17.5 220 45 97 335 76 26.5 20.1 24.0 pl at 24C

5.0, 5.1 5.4, 5.5, 5.6 3.50 5.30 5.85 6.55 5.40 6.0, 6.3, 6.6 7.00 10.25 4.23, 4.38, 4.50 8.3, 8.5 4.15

Electrophoresis performed in Tris-glycine-SDS buffer. *The larger proteins fail to move significantly into the gel. NOTE: Proteins smaller than approximately 14 kD fail to resolve away from the ion front in a Tris-glycine buffer system. A Tris-Tricine method is routinely used instead (Schagger, H. and Von Jagow, G. (1987). Analytical Biochem. 166, 368)

Recommended acrylamide concentrations for semi-dry transfer of proteins from a 0.75-mm thick gel*
Mr (x103) % Acrylamide < 20 20% < 36 15% 14 - 66 10% 29 - 150 5% *Reference Smejkal, G. and Gallagher, S., BioTechniques 16, 197 (1994).

8.15, 8.45, 8.65 6.85, 7.25 4.7 2.8

220 45 97 669 76 53 20.1

5.8, 6.2, 7.0, 7.7, 8.0 4.55 9.3

* Indicates protein intended use: S, SDS PAGE; I, IEF; N, non-denaturing PAGE

Nucleic Acid Electrophoresis


Recommended agarose concentrations for DNA separation*
Amount of agarose in gel (% [w/v]) 0.3 0.6 0.7 0.9 1.2 1.5 2.0 Effective range of separation of linear DNA molecules (kb) 5 - 60 1 - 20 0.8 - 10 0.5 - 7 0.4 - 6 0.2 - 3 0.1 - 2

Recommended acrylamide concentrations for DNA separation*


Acrylamide (% [w/v]) 3.5 5.0 8.0 12.0 15.0 20.0 Effective range Xylene of separation cyanol Bromophenol (bp) FF blue 1000 - 2000 80 - 500 60 - 400 40 - 200 25 - 150 6 - 100 460 260 160 70 60 45 100 65 45 20 15 12

Migration rates of marker dyes through denaturing polyacrylamide gels*


% Bromophenol Xylene cyanol Polyacrylamide blue FF 5 6 8 10 20
The

35 26 19 12 8

130 106 76 55 28

*From Molecular Cloning: A Laboratory Manual (Sambrook, J., Fritsch, E.F. and Maniatis, T., third edition, 2001).

is included at 1/30th the concentration of acrylamide. The numbers given are the approximate sizes (in nucleotide pairs) of fragments of double-stranded DNA with which the dye comigrates. *From Molecular Cloning: A Laboratory Manual (Sambrook, J., Fritsch, E.F. and Maniatis, T., third edition, 2001).

N,N-methylenebisacrylamide

numbers are the approximate sizes of DNA (in nucleotides) with which the marker dye comigrates. *From Molecular Cloning: A Laboratory Manual (Sambrook, J., Fritsch, E.F. and Maniatis, T., third edition, 2001).

Common Conversions
63
Technical Appendix

1 kb of DNA = 37,000 g/mol Average molecular weight of one amino acid = 120 g/mol

Quantitation
DQ 300 FLUOROMETER DNA/RNA QUANTITATION

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65
Contact Information

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66

BIBLIOGRAPHY

Bibliography
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Burlando, B, et. al., Essential Role of Ca+2 -Dependent Phospholipase A2 in Estradiol-Induced Lysosome Activation. American Journal of Physiology Cell Physiology 283(5): C1461-C1468, November 2002. Chen, W-C, et. al., Purification and Characterization of a Pectin Lyase from Pythium splendens Infected Cucumber Fruits. Botanical Bulletin of Academia Sinica 39:181-186, 1998. Chung, W-L, et. al., Cytoplasmic Targeting Signals in Transmembrane Invariant Surface Glycoproteins of Trypanosomes. Journal of Biological Chemistry 279(52):54887-54895, 24 Dec 2004. Couce, ME, et. al., Analysis of K-ras, N-ras, H-ras, and p53 in Lung Neuroendocrine Neoplasms. Diagnostic Molecular Pathology 8(2):71-79, Jun 1999. de la Cruz, M, et. al., DNA Isolation and Amplification from Cacti. Plant Molecular Biology Reporter 15:319-325, 1997. Diehl, S, et. al., Analysis of Stage-Specific Gene Expression in the Bloodstream and the Procyclic Form of Trypanosoma brucei Using a Genomic DNA Microarray. Molecular & Biochemical Parasitology 123:115-/123, 2002. Driscoll, MD, et. al., Sequence Requirements for Estrogen Receptor Binding to Estrogen Response Elements. Journal of Biological Chemistry 273(45): 29321-29330, 6 Nov 1998. Engelbrecht, CJB, et. al., Genetic Variation in Eastern North American and Putatively Introduced Populations of Ceratocystis fimbriata f. platani. Molecular Ecology 13:2995-3005, 2004. Ewings EL, et. al., Genotoxic and Functional Consequences of Transplacental Zidovudine Exposure in Fetal Monkey Brain Mitochondria. Journal of Acquired Immune Deficiency Syndromes 24(2):100-105, 1 June 2000. Gao, Z, et. al., Palmitoylation of the Recombinant Human A1 Adenosine Receptor: Enhanced Proteolysis of Palmitoylation-Deficient Mutant Receptors. Biochemical Journal 342:387-395, 1999. Gerber, I, et. al., Stimulatory Effects of Creatine on Metabolic Activity, Differentiation and Mineralization of Primary Osteoblast-Like Cells in Monolayer and Micromass Cell Cultures. European Cells and Materials 10:8-22, 2005. Gerschenson, M, et. al., Fetal Mitochondrial Heart and Skeletal Muscle Damage in Erythrocebus patas Monkeys Exposed in Utero to 3'-Azido3'-Deoxythymidine. AIDS Research and Human Retroviruses 16(7):635-44, 01 May 2000. Goodwin, SB, et. al., DNA Fingerprint Probe from Mycosphaerella graminicola Identifies an Active Transposable Element. Phytopathology 91(12):1181-1188, 2001. Gorton, TS, et. al., Physical Mapping of the Mycoplasma gallisepticum S6 Genome with Localization of Selected Genes. Journal of Bacteriology 177(1):259-263, Jan 1995. Gruffat, D, et. al., Developmental Changes in Apolipoprotein B Gene Expression in the Liver of Fetal Calves. Biology of the Neonate 74:233242, 1998. Hanson, DC, et. al., UK-78,282, a Novel Piperidine Compound that Potently Blocks the Kv1.3 Voltage-Gated Potassium Channel and Inhibits Human T Cell Activation. British Journal of Pharmacology 126(8):1707-16, Apr 1999. Hanson, SF, and Maxwell, DP, trans-Dominant Inhibition of Geminiviral DNA Replication by Bean Golden Mosaic Geminivirus rep Gene Mutants. Phytopathology 89(6):480-486, 1999. Hu, J, and Bock, D, A Cell Culture Medium that Supports the Differentiation of Human Retinal Pigment Epithelium into Functionally Polarized Monolayers. Molecular Vision 7:14-19, 7 February 2001. Hu, L, et. al., Two-Dimensional Protein Database of Human Pancreas. Electrophoresis 25:512518, 2004. Krieg A, et. al., Expression of Different Survivin Variants in Gastric Carcinomas: First Clues to a Role of Survivin-2B in Tumour Progression. British Journal of Cancer 86(5):737-43, 4 Mar 2002. Lai, I-R, et. al., The Protective Role of Heme Oxygenase-1 on the Liver after Hypoxic Preconditioning in Rats. Transplantation 77(7):10041008, 15 April 15 2004. Lee, SG, and Calhoun, DH, Urease from a Potentially Pathogenic Coccoid Isolate: Purification, Characterization, and Comparison to Other Microbial Ureases. Infection and Immunity 65(10):3991-3996, Oct 1997. Lin, C-J, and Smith, DE, Glycylsarcosine Uptake in Rabbit Renal Brush Border Membrane Vesicles Isolated from Outer Cortex or Outer Medulla: Evidence for Heterogeneous Distribution of Oligopeptide Transporters. AAPS PharmSci 1(2):article 1, 1999. Lindberg, T, et. al., In Vitro Digestion of Proteins in Human Milk Fortifiers and in Preterm Formula. Journal of Pediatric Gastroenterology & Nutrition. 27(1):30-36, July 1998. Liou, Y-M, et. al., Altered Expression of Cardiac Myosin Isozymes Associated with the Malignant Hyperthermia Genotype in Swine. Anesthesiology 93(5):1312-1319, Nov 2000. Lu, K, et. al., Antimicrobial Resistance Gene Delivery in Animal Feeds. Emerging Infectious Diseases Journal [serial online] 10(4), Apr 2004 (date cited). Available from http://www.cdc.gov/ncidod/EID/vol10no4/03-0506.htm.

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Mahmood, K, et. al., Human Cytomegalovirus Plasmid-Based Amplicon Vector System for Gene Therapy. Genetic Vaccines and Therapy 3:1, 2005. Mattson, JP, et. al., Induction of Mitochondrial Stress Proteins Following Treadmill Running. Medicine & Science in Sports & Exercise 32(2):365-369, Feb 2000. Matyasek, R, et. al., NTRS, a New Family of Highly Repetitive DNAs Specific for the T1 Chromosome of Tobacco. Chromosoma 106:369379, 1997. Melody, JL, et. al., Early Postmortem Biochemical Factors Influence Tenderness and Water-Holding Capacity of Three Porcine Muscles. Journal of Animal Science 82:1195-1205, 2004. Menell, JS, et. al., Annexin II and Bleeding in Acute Promyelocytic Leukemia. New England Journal of Medicine 340(13):994-1004, 1 Apr 1999. Moneo, I, et. al., Immunodetection of Specific IgE (Immunoblotting) in a Prevalence Study of Anisakis simplex Sensitisation in Spain. Alergologia e Inmunologia Clinica 15:255-61, 2000. Narayanan, S, et. al., Comparative Evaluation of PCR using IS6110 and a New Target in the Detection of Tuberculous Lymphadenitis. Current Science 78(11):1367-1370, 10 Jun 2000. Noriega, R, et. al., Ecdysteroids and Oocyte Development in the Black Fly Simulium vittatum. BMC Developmental Biology [serial online] 2:6, 24 Apr 2002. Available from http://www.biomedcentral.com/1471213X/2/6. Park, O, et. al., Calcium Binding of Phosphopeptides Derived from Hydrolysis of -Casein or -Casein Using Immobilized Trypsin. Journal of Dairy Science 81(11):2850-2857, 1998. Patocs, A, et. al., Clinical and Biochemical Features of Sporadic and Hereditary Phaeochromocytomas: an Analysis of 41 Cases Investigated in a Single Endocrine Centre. European Journal of Cancer Prevention 13(5):403-409, October 2004. Rollo, EE, et. al., The Cytokine Osteopontin Modulates the Severity of Rotavirus Diarrhea. Journal of Virology 79(6): 35093516, Mar 2005. Sarmiento, M, High Resolution, Standard Format Two-Dimensional Protein Electrophoresis using Disposable Glass Micropipettes and NonDedicated Equipment. Electrophoresis 16(1):431-437, 1995. Schubeler, D, et. al., Genomic Targeting of Methylated DNA: Influence of Methylation on Transcription, Replication, Chromatin Structure, and Histone Acetylation. Molecular and Cellular Biology 20(24):91039112, Dec 2000. Skorska, C, et. al., Effects of Repeated Inhalation Exposure to Microvesicle-Bound Endotoxin. Annals of Agricultural and Environmental Medicine 3:61-65, 1996. Theopold, U, et. al., CalpA, a Drosophila Calpain Homolog Specifically Expressed in a Small Set of Nerve, Midgut, and Blood Cells. Molecular and Cellular Biology 15(2): 824834, Feb 1995.

Tio, PH, et. al., Two Dimensional VOPBA Reveals Laminin Receptor (LAMR1) Interaction with Dengue Virus Serotypes 1, 2 and 3. Virology Journal 2:25, 25 March 2005. Ueno, M, et. al., A High-Fructose Diet Induces Changes in pp185 Phosphorylation in Muscle and Liver of Rats. Brazilian Journal of Medical and Biological Research 33(12):1421-1427, Dec 2000. Volk, GM, and Franceschi, VR, Localization of a Calcium Channel-Like Protein in the Sieve Element Plasma Membrane. Australian Journal of Plant Physiology 27(9):779-784, 2000. Yamamoto, K, et. al., Experimental Infection of Domestic Cats with Bartonella koehlerae and Comparison of Protein and DNA Profiles with Those of Other Bartonella Species Infecting Felines. Journal of Clinical Microbiology 40(2):466-474, Feb 2002. Zhou, J, et. al., RNA Isolation without Gel Formation from Oligosaccharide-Rich Onion Epidermis. Plant Molecular Biology Reporter 17: 397407, 1999.

Please note that we have a large publications database that cannot be accommodated in this catalog. For references on specific applications please contact Hoefer at support@hoeferinc.com.

For theory and practice of a wide range of electrophoresis techniques Hoefer recommends Dr. Reiner Westermeier's Electrophoresis in Practice published by Wiley-VCH (ISBN 3-527-30354-5).

Bibliography

68

CUSTOM PRODUCTS

Custom Products

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Hoefer has been pioneering design and manufacture of electrophoresis electrophoresis apparatus and related equipment for biotechnology research for over three over decades. We have the skilled craftsmen and state of the art equipment to art manufacture manufacture the products described in this catalog. products described catalog If you need parts or products similiar to those found in this catalog or have a new design idea that needs to be materialized; please contact us and we will be happy to produce it for you. We accept your information as: CAD files Paper drawings A thorough verbal description (for simple parts or assemblies) In addition to our CNC machining capabilities, Hoefers custom machine shop boasts: Programming with Mastercam 9.1 and Geopath 4.1, and compatibility with most popular CAD software Conventional milling with Bridgeport J series mills Conventional turning with Clausing Colchester, 15" swing lathes Manually operated sawing, drilling, and tapping machines Finish options including bead blasting and buffing Plastic fabricating in our wellequipped solvent bonding room Call on our experts. Well analyze your requirements, determine the most efficient way to meet them, and offer a price you will like.

69

Custom Products

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