PATIENT ONE: 6 year old boy 1) Is this a primary or secondary disorder?

2) What symptoms and tests support your response for a primary or secondary disorder? 3) Is this an inherited or acquired disorder? 4) What symptoms and history supports your response for an inherited or acquired disorder? 5) If acquired, state condition or medication that may be causing the patients problem? 6) What are the diseases or syndromes which need to be ruled out before the diagnosis of this patient can be made? 7) What does the aggregation patterns tell the physician about this patient problem? 8) How does each of the aggregation patterns confirm or rule out the patients problem? a) ADP? b) Epinephrine? c) Collagen? d) Ristocetin? 9) What would Arachidonic Acid aggregation pattern demonstrate? 10) If an PFA was performed, the results would normal or abnormal for the : a) Col/EPI CT = _____________ b) Col/ADP CT = _____________ 11) If inherited what is the defect? (i.e.; GPIb defect) 12) What would the result of the reptilase Time be? Normal or Abnormal. 13) What would the result of the B. atrox Time be? Normal or Abnormal. 14) What would the result of the DRVVT assay be? Normal or Abnormal. 15) What would the result of the PNP or Hexagonal PNP be? Normal or Abnormal. 16) Would the Bethesda Assay be beneficial? Why? 17) What Fibrinolysis assays are recommended? 18) What in-vivo inhibitor assays are indicted? 19) What is the patients diagnosis? 20) What treatment options are available? a) Blood products? b) Medications? Page 1 of 5

CL 4145 Coagulation Case Studies Answer Sheet PATIENT TWO: 19 year old man 1) Is this a primary or secondary disorder? 2) What symptoms and tests support your response for a primary or secondary disorder? 3) Is this an inherited or acquired disorder? 4) What symptoms and history supports your response for an inherited or acquired disorder? 5) If acquired, state condition or medication that may be causing the patients problem? 6) What are the diseases or syndromes which need to be ruled out before the diagnosis of this patient can be made? 7) What does the aggregation patterns tell the physician about this patient problem? 8) How does each of the aggregation patterns confirm or rule out the patients problem? a) ADP? b) Epinephrine? c) Collagen? d) Ristocetin? 9) What would Arachidonic Acid aggregation pattern demonstrate? 10) If an PFA was performed, the results would normal or abnormal for the : a) Col/EPI CT = _____________ b) Col/ADP CT = _____________ 11) If inherited what is the defect? (i.e.; GPIb defect) 12) What would the result of the reptilase Time be? Normal or Abnormal. 13) What would the result of the B. atrox Time be? Normal or Abnormal. 14) What would the result of the DRVVT assay be? Normal or Abnormal. 15) What would the result of the PNP or Hexagonal PNP be? Normal or Abnormal. 16) Would the Bethesda Assay be beneficial? Why? 17) What Fibrinolysis assays are recommended? 18) What in-vivo inhibitor assays are indicted? 19) What is the patients diagnosis? 20) What treatment options are available? a) Blood products? b) Medications? PATIENT THREE: 61 year old woman Page 2 of 5

CL 4145 Coagulation Case Studies Answer Sheet 1) Is this a primary or secondary disorder? 2) What symptoms and tests support your response for a primary or secondary disorder? 3) Is this an inherited or acquired disorder? 4) What symptoms and history supports your response for an inherited or acquired disorder? 5) If acquired, state condition or medication that may be causing the patients problem? 6) What are the diseases or syndromes which need to be ruled out before the diagnosis of this patient can be made? 7) What does the aggregation patterns tell the physician about this patient problem? 8) How does each of the aggregation patterns confirm or rule out the patients problem? a) ADP? b) Epinephrine? c) Collagen? d) Ristocetin? 9) What would Arachidonic Acid aggregation pattern demonstrate? 10) If an PFA was performed, the results would normal or abnormal for the : a) Col/EPI CT = _____________ b) Col/ADP CT = _____________ 11) If inherited what is the defect? (i.e.; GPIb defect) 12) What would the result of the reptilase Time be? Normal or Abnormal. 13) What would the result of the B. atrox Time be? Normal or Abnormal. 14) What would the result of the DRVVT assay be? Normal or Abnormal. 15) What would the result of the PNP or Hexagonal PNP be? Normal or Abnormal. 16) Would the Bethesda Assay be beneficial? Why? 17) What Fibrinolysis assays are recommended? 18) What in-vivo inhibitor assays are indicted? 19) What is the patients diagnosis? 20) What treatment options are available? a) Blood products? b) Medications?

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CL 4145 Coagulation Case Studies Answer Sheet PATIENT FOUR: 25 year old woman 1) Is this a primary or secondary disorder? 2) What symptoms and tests support your response for a primary or secondary disorder? 3) Is this an inherited or acquired disorder? 4) What symptoms and history supports your response for an inherited or acquired disorder? 5) If acquired, state condition or medication that may be causing the patients problem? 6) What are the diseases or syndromes which need to be ruled out before the diagnosis of this patient can be made? 7) What does the aggregation patterns tell the physician about this patient problem? 8) How does each of the aggregation patterns confirm or rule out the patients problem? a) ADP? b) Epinephrine? c) Collagen? d) Ristocetin? 9) What would Arachidonic Acid aggregation pattern demonstrate? 10) If an PFA was performed, the results would normal or abnormal for the : a) Col/EPI CT = _____________ b) Col/ADP CT = _____________ 11) If inherited what is the defect? (i.e.; GPIb defect) 12) What would the result of the reptilase Time be? Normal or Abnormal. 13) What would the result of the B. atrox Time be? Normal or Abnormal. 14) What would the result of the DRVVT assay be? Normal or Abnormal. 15) What would the result of the PNP or Hexagonal PNP be? Normal or Abnormal. 16) Would the Bethesda Assay be beneficial? Why? 17) What Fibrinolysis assays are recommended? 18) What in-vivo inhibitor assays are indicted? 19) What is the patients diagnosis? 20) What treatment options are available? a) Blood products? b) Medications? Page 4 of 5

CL 4145 Coagulation Case Studies Answer Sheet PATIENT FIVE: 27 year old woman 1) Is this a primary or secondary disorder? 2) What symptoms and tests support your response for a primary or secondary disorder? 3) Is this an inherited or acquired disorder? 4) What symptoms and history supports your response for an inherited or acquired disorder? 5) If acquired, state condition or medication that may be causing the patients problem? 6) What are the diseases or syndromes which need to be ruled out before the diagnosis of this patient can be made? 7) What does the aggregation patterns tell the physician about this patient problem? 8) How does each of the aggregation patterns confirm or rule out the patients problem? a) ADP? b) Epinephrine? c) Collagen? d) Ristocetin? 9) What would Arachidonic Acid aggregation pattern demonstrate? 10) If an PFA was performed, the results would normal or abnormal for the : a) Col/EPI CT = _____________ b) Col/ADP CT = _____________ 11) If inherited what is the defect? (i.e.; GPIb defect) 12) What would the result of the reptilase Time be? Normal or Abnormal. 13) What would the result of the B. atrox Time be? Normal or Abnormal. 14) What would the result of the DRVVT assay be? Normal or Abnormal. 15) What would the result of the PNP or Hexagonal PNP be? Normal or Abnormal. 16) Would the Bethesda Assay be beneficial? Why? 17) What Fibrinolysis assays are recommended? 18) What in-vivo inhibitor assays are indicted? 19) What is the patients diagnosis? 20) What treatment options are available? a) Blood products? b) Medications? Page 5 of 5