Code No: RR222304 Set No.

1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTATION METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. (a) What are the suitable instrumental methods used for the analysis of
i. gaseous mixtures,
ii. alloys and ores
iii. traces of metal ions
(b) Write notes on
i. Comparison with standards
ii. Standard addition method
(c) Write notes on
i. Problems in analysis
ii. Methods of analysis [5+5+6]

2. (a) Define the following terms: Photometer, Spectrophotometer and Colorimeter.

(b) Define Lambert’s law and Beer’s law and derive the equations.
(c) What is the difference between molar absorptivity and absorbance? [6+6+4]

3. (a) Calculate the frequency of radiation whose wavelength is 400 nm. Express
this wavelength in wave number.
(b) Calculate the wave number of the radiation if the frequency is 2.06 × 1014 Hz
given c = 3.0 × 1010 cm/sec
(c) Calculate the wavelength of electron moving with one third of the speed of
light. [4+4+4+4]

4. (a) How will you determine metallic elements in food industry using Atomic ab-
sorption spectroscopy?
(b) How will you determine Ca, Mg, Na, and K in blood serum using Atomic
absorption spectroscopy? [8+8]

5. Write briefly on

(a) Schematic and function of laser microscope

(b) Quantitative analysis
(c) Photographic plates
(d) Echelle monochromators. [16]

6. Draw a schematic diagram of an nmr instrument and discuss the parts [16]

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Code No: RR222304 Set No. 1
7. Discuss ESR instrumentation with a block diagram. [16]

8. Discuss theory and practice of HPLC. [16]

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Code No: RR222304 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTATION METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What are the instrumental methods? How are they classified? Name two or three
instrumental methods for each of the physical property. [16]

2. (a) Why water can’t be used as a solvent for infrared spectroscopy?

(b) Which crystals are used for optical systems in infrared instruments?
(c) Which selection rule is used in IR spectroscopy?
(d) Why far infra red region and near infra red regions are not much of use for
spectroscopic purposes? [4+4+4+4]

3. (a) Why are complexes used to quantitatively determine metal ions?

(b) What are the requirements of complex ions to be used in a spectrophotometric
determinations? [8+8]

4. (a) Describe the procedure for quantitaton in Atomic absorption spectroscopy

(b) Why is the internal standard procedure seldom used in atomic absorption
determination?
(c) Why the technique of atomic absorption is only limited to metals? [8+4+4]

5. Write an assay on plasma excitation sources. [16]

6. What is nmr? Explain the properties of nuclei [16]

7. Discuss aminoacids separation by paper chromatography. [16]

8. What is the Van Deemeter Equation? In a column 6 ft long A=0.1, B=0.05, C=0.2
and V=0.1 ft/sec. What is the theoretical plate height? How many theoretical
plates are in the column? [16]

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Code No: RR222304 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTATION METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Define sensitivity and detection limits.

(b) Give the detection limits of
i. Gas chromatography
ii. I.R. spectrophotometry
iii. U.V. spectrophotometry
iv. NMR
v. Mass Spectrometry
(c) What are the advantages and disadvantages of chemical and instrumental
methods. [6+5+5]

2. Write short notes on :

(a) Molar extinction coefficient

(b) laws of absorption
(c) Photometric titrations
(d) Photometric accuracy. [16]

3. Write short notes on the following UV applications:

(a) Quantitative analysis

(b) Molecular Weight determination
(c) Impurities in organic compounds. [6+6+4]

4. (a) Fluctuation in flame temperature can be considered a physical interference.

Discuss the relative seriousness of flame temperature fluctuations in flame
emission and atomic absorption spectroscopy.
(b) Why so turbulent flow burners never strike back?
(c) How will you determine lead in petrol by of atomic absorption spectroscopy
[6+4+6]

5. (a) Explain briefly the electrical sources.

(b) How the samples are introduced? [8+8]

6. Write notes on:

(a) Proton exchange

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Code No: RR222304 Set No. 3
(b) Magnet in nmr
(c) RF Generator
(d) Detector [16]

7. What are the principal analytical uses of column chromatography? [16]

8. Describe three detectors with diagrams used in GC. [16]

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Code No: RR222304 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTATION METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) How is relative error expressed?

(b) Define the term mean and median. Under what condition does the median
become equal to the mean?
(c) What is the difference between atomic and molecular spectroscopy?
(d) What is the effect of determinate error on normal error curve? [4+4+4+4]
2. Write short notes on:
(a) Requirements of a UV radiation source
(b) Hydrogen discharge lamp
(c) Deuterium lamps
(d) Xenon discharge lamps. [4+4+4+4]
3. (a) Discuss the origin of color in organic compounds. What are chromophores
and auxochromes?
(b) Explain with suitable examples ,the meaning of blue and red shifts. [8+8]
4. (a) Write the advantages of atomic absorption spectroscopy over flame emission
spectroscopy?
(b) What is a single beam and a double beam atomic absorption spectrophotome-
ter and explain the instrumentation involved?
(c) Define sensitivity and detection limits in atomic absorption spectroscopy. [6+6+4]
5. Explain briefly
(a) Qualititative analysis
(b) Line reversal
(c) Matrix effects
(d) Preburn time. [16]
6. Draw and discuss the nmr spectrum of ethanol [16]
7. Describe TLC technique with suitable examples. [16]
8. Discuss the principles and applications of Gas chromatography. [16]

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