DAFTAR ISI

HALAMAN SAMPUL DEPAN .............................................................

HALAMAN JUDUL ..............................................................................
HALAMAN PENGESAHAN PEMBIMBING .....................................

ii

HALAMAN PENGESAHAN PENGUJI .............................................

iii

HALAMAN PERNYATAAN KEASLIAN PENELITIAN .................

iv

HALAMAN PERSEMBAHAN .............................................................

HALAMAN MOTTO .............................................................................

vi

KATA PENGANTAR ............................................................................

vii

DAFTAR ISI ...........................................................................................

ix

DAFTAR GAMBAR ..............................................................................

xi

DAFTAR TABEL ..................................................................................

xii

DAFTAR LAMPIRAN ..........................................................................

xiii

INTISARI ...............................................................................................

xiv

ABSTRACT ............................................................................................

xv

BAB I PENDAHULUAN
A. Latar Belakang Masalah..........................................................

B. Perumusan Masalah ................................................................

C. Tujuan Penelitian ....................................................................

D. Manfaat Penelitian ..................................................................

BAB II STUDI PUSTAKA

A. Tinjauan Pustaka .....................................................................

1. Tinjauan tentang mimba....................................................

2. Nimbidin ...........................................................................

3. Ekstraksi dengan alat Soxhlet ...........................................

4. Propionibacterium acnes ..................................................

5. Jerawat ..............................................................................

12

6. Benzoil peroksida ..............................................................

13

7. Antibakteri ........................................................................

14

8. Pemeriksaan mikrobiologi (Pengujian aktivitas antibakteri)

...........................................................................................

ix

14

9. Sterilisasi ...........................................................................

16

10. Uraian tentang Kromatografi Lapis Tipis .........................

16

B. Landasan Teori ........................................................................

21

C. Hipotesis..................................................................................

22

BAB III METODE PENELITIAN

A. Alat dan Bahan .......................................................................

23

B. Jalannya Penelitian .................................................................

24

1. Determinasi tanaman ........................................................

24

2. Penyiapan serbuk kulit batang mimba ..............................

24

3. Pembuatan ekstrak tanaman..............................................

24

4. Uji aktivitas antibakteri .....................................................

25

5. Uji kandungan senyawa dengan metode KLT ..................

28

C. Analisis Hasil ..........................................................................

28

BAB IV HASIL DAN PEMBAHASAN

A. Hasil dan Pembahasan..............................................................

32

BAB V KESIMPULAN DAN SARAN

A. Kesimpulan ...............................................................................

45

B. Saran .........................................................................................

45

DAFTAR PUSTAKA ..............................................................................

46

LAMPIRAN ............................................................................................

49

DAFTAR GAMBAR

Gambar 1. Struktur nimbidin ..................................................................

Gambar 2. Propionibacterium acnes ......................................................

10

Gambar 3. Penampang kulit ....................................................................

11

Gambar 4. Benzoil peroksida ..................................................................

13

Gambar 5. Skema penyarian bahan.........................................................

29

Gambar 6. Skema uji mikrobiologi .........................................................

30

Gambar 7. Skema identifikasi kandungan senyawa kimia dengan KLT

31

Gambar 8. Grafik hubungan diameter hambatan terhadap P. acnes dan

konsentrasi ekstrak kulit batang mimba ................................

40

Gambar 9. Hasil uji mikrobiologi fraksi air ............................................

40

Gambar 10. Hasil uji mikrobiologi ekstrak etanol ....................................

41

Gambar 11. Hasil uji mikrobiologi fraksi etil asetat .................................

41

Gambar 12. Kromatogram ........................................................................

43

xi

DAFTAR TABEL

Tabel I.

Hasil penyarian kulit batang tanaman mimba dengan

menggunakan penyari etanol 70 %, petroleum eter,
etil asetat dan air......................................................................

Tabel II.

36

Uji aktivitas antibakteri kulit batang tanaman

mimba dengan berbagai kadar terhadap Propionibacterium acnes
................................................................................................. 39

Tabel III. Perbedaan hRf masing-masing spot ........................................

xii

43

DAFTAR LAMPIRAN

Lampiran 1. Gambar pohon mimba .........................................................

50

Lampiran 2. Gambar alat Soxhlet ............................................................

50

Lampiran 3. Gambar stok Bakteri P. acnes .............................................

51

Lampiran 4. Gambar Standard Brown .....................................................

51

Lampiran 5. Gambar autoklaf ..................................................................

52

Lampiran 6. Gambar Laminar Air Flow ..................................................

52

Lampiran 7. Gambar alkalis............... ......................................................

53

Lampiran 8. Gambar Gas pack ................................................................

53

Lampiran 9. Gambar Inkubator ................................................................

54

Lampiran 10.Surat determinasi tanaman...................................................

55

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