RESUME

Mr.Santhosh Nagaraj N. santhoshnagaraj1@gmail.com #65, Nanjundeshwara nilaya Devarachikkanahalli, IIMB [P], Bannerghatta road, Bangalore-76

Email: Mobile: 9632221960

CARRIER OBJECTIVE Seeking a position in an industry with a professional working environment which provides an opportunity to utilize my skills and abilities and enriches my competences, thus fulfilling the vision of organization.

EDUCATION
M.Sc Biotechnology:Govt Science College, Bangalore university 2010. Aggregate 71%. B.Sc (CZBt) : BHS First grade college, Bangalore University 2008. Aggregate 73%. PUC (PCMB): Vivekananda PU College, Bangalore University 2005.Aggregate 63%. SSLC: Nightingales English school, 2003. Aggregate 76%

WORK EXPERIENCE Working as a SCIENTIST (R&D-MOLECULAR BIOLOGY DEPARTMENT) in BHAT BIOTECH INDIA (P) LTD., - From AUG 2010 to TILL DATE (1 YEAR EXPERIENCE)
1

ALSO WORKING AS A TEACHING KIT INCHARGE

TECHNICAL EXPERTISE FOLLOWING ARE THE TEACHING KITS STANDARDISED. 1. PCR. 2. Genomic DNA isolation from fungi.

3. RAPD 4. Sandwich ELISA 5. Antibody Capturing ELISA 6. Bacterial Conjugation 7. Immuno Precipitation. 8. Bacterial Transduction.

PROJECTS HANDLED IN BHAT BIOTECH INDIA (P) LTD. 1. MOLECULAR CLONING AND EXPRESSION OF HCV NS3 IN E.Coli CELLS AND PURIFICATION OF THE PROTEIN.

2. MOLECULAR CLONING AND EXPRESSION OF SYPHILIS 47 KDa LIPOPROTEIN IN E.Coli CELLS AND PURIFICATION OF THE PROTEIN FOR THE DELELOPMENT OF DIAGNOSTIC KIT.

3. MOLECULAR CLONING AND EXPRESSION OF STREPTAVIDIN IN E.Coli CELLS AND PURIFICATION OF THE PROTEIN FOR CONJUGATION WITH HRP.

PROFICIENT IN PERFORMING FOLLOWING METHODS (Teaching Kit)

1. Blotting techniques (Western, Southern). 2. Elisa (sandwich, DOT Elisa, Antibody capturing (Indirect)). 3. RAPD, PCR, RT-PCR. 4. Bacterial Conjugation, Bacterial Transformation, Bacterial Transduction. 5. Competent cells preparation(Calcium Chloride, PEG Method). 6. Affinity chromatography(Ni-NTA) . 7. Quantification of DNA, RNA and Proteins. 8. Ouchterlony Double Diffusion(ODD), Radial Immuno Diffusion(RID). 9. Rocket Immuno Electrophoresis(RIEP), Counter current immuno electophoresis(CCIE), Immuno electrophoresis(IEP). 10. Phage titration, Protein Silver staining. 11. VDRL, WIDAL TEST, LATEX AGGLUTINATION, Quantitative precipitation. 12. Restriction Digestion, Ligation, Immuno precipitation, IgG Isolation, Purification with Ni NTA Affinity Chromatography and Dialysis.

PROFICIENT IN HANDLING FOLLOWING INSTRUMENTS

1. PCR Machine (Thermocycler). 2. Elisa Reader. 3. Spectrophotometer. 4. Ni- NTA Chromatography.

COMPUTER SKILLS

VBA (Visual Basic Programming).

Oracle 9 i.

SQL .

DECLARATION I hereby declare that the information furnished above is true to the best of my knowledge and belief.
Date: 05-08-11 N.) Place: Bangalore (Santhosh Nagaraj