TRANSFUSION MEDICINE UNIT, HOSPITAL UNIVERSITI SAINS MALAYSIA, KELANTAN TITLE : LIS TEST CODE PROCEDURE NO.

HUSM/HEMA-UPT/STM-R5 FULL BLOOD COUNT (FBC) ISSUE NO. VERSION NO. ISSUE DATE. 1 1 01.04.2010

APPROVED BY:

.. ASSOC. PROF. DR ROSLINE HASSAN HEAD OF HAEMATOLOGY DEPARTMENT

CONTROLLED COPY NO: 1 REGISTERED HOLDER HAEMATOLOGY LAB

RECORD OF REVIEW/AMMENDMENT DATE VERSION NO. ISSUE NO. DETAIL OF AMMENDMENT BY

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STANDARD TEST MANUAL

TRANSFUSION MEDICINE UNIT, HOSPITAL UNIVERSITI SAINS MALAYSIA, KELANTAN TITLE : LIS TEST CODE PROCEDURE NO.
PREPARED BY DESIGNATION CHECKED BY DESIGNATION AUTHORISED BY DESIGNATION

FULL BLOOD COUNT (FBC)

ISSUE NO. VERSION NO.

1 1 01.04.2010

HUSM/HEMA-UPT/STM-R5

ISSUE DATE.

: SUREHAN SULONG : MEDICAL LABORATORY TECHNOLOGIST U29 : MR LIM SENG HOCK : SCIENTIFIC OFFICER : ASSOC. PROF. DR ROSLINE HASSAN : HAEMATOLOGIST/LAB DIRECTOR

1.

OBJECTIVE
To perform full blood count using SYSMEX KX-21 haematology analyzer

2.

METHOD
Automatic multi-parameter blood cell counters with three detector blocks. Two kinds of reagent for blood analysis.

3.

PRINCIPLE
Refer Sysmex Operators Manual for KX-21

4.

REQUIREMENTS
4.1 4.1.1 4.2 4.2.1 Sample Venous blood sample in EDTA container (2 ml / 3 ml) Reagents Cellpack PK 30L Store EPK at +5 - +30 C If the cubitainer is unopened. EPK can be used up to the expiry date shown on the cubitainer. Once open (connected to the instrument), product stability in the container is 60 days maximum. 4.2.2 STROMATOLYSER WH SWH-200

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STANDARD TEST MANUAL

TRANSFUSION MEDICINE UNIT, HOSPITAL UNIVERSITI SAINS MALAYSIA, KELANTAN TITLE : LIS TEST CODE PROCEDURE NO. HUSM/HEMA-UPT/STM-R5 FULL BLOOD COUNT (FBC) ISSUE NO. VERSION NO. ISSUE DATE. 1 1 01.04.2010

4.2.3 QC MATERIAL * Sysmex Eightcheck (High) 3WP - code no 7274 *Sysmex Eightcheck (Low) 3WP - code no 7275 *Sysmex Eightcheck (Normal) 3WP code no 7276

5.
NO

PROCEDURE
ACTIVITY Manual Mode Analysis (KX-21) 5.1.1 5.1.2 5.1.3 5.1.4 5.1.5 5.1.6 5.1.7 5.1.8 Make sure the instrument is in the READY status. Press [SAMPLE NO.] key to input sample ID # using the numeric keypad. Press [ENTER] key, the instrument LCD screen will display READY. Mix the sample blood thoroughly by inversion several times. Remove the cap carefully, and then set the sample into the manual aspiration pipette. Press the [START] switch. Remove the blood sample after sound beeps twice. Results are displayed on the LCD screen and can be automatically printed out RESPONSIBILITY JTMP/PS

5.1

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STANDARD TEST MANUAL

TRANSFUSION MEDICINE UNIT, HOSPITAL UNIVERSITI SAINS MALAYSIA, KELANTAN TITLE : LIS TEST CODE PROCEDURE NO. HUSM/HEMA-UPT/STM-R5 FULL BLOOD COUNT (FBC) ISSUE NO. VERSION NO. ISSUE DATE. 1 1 01.04.2010

6.

LIMITATION 6.1 Hemoglobin measurements may be falsely elevated due to the influences of abnormal samples (e.g. leukocytosis above 100,000/uL) and lipemia samples. 6.2 The haemoglobin method available for this analyzer cannot detect sulfhaemoglobin, verdohaemoglobin, choleglobin or other unusual degradation products of haemoglobin. 6.3 WBC results may be elevated erroneously due to unlysed red cells in patients with hemoglobinopathies, severe liver disease or in neonates.

7.

REFERENCE RANGE
Refer appendix 1 PANIC VALUES FOR FBC PARAMETER LOW CRITICAL VALUE HIGH CRITICAL VALUE

HAEMOGLOBIN TOTAL WHITE CELL PLATELET 8. RESULT

5 gm/dl 1.0 x10 9 L 20 X 10 9/L

21 gm/dl 60.0 x 10 9 /L 1000x 10 9 /L

The results are released according to the test requested

9.

REFERENCES
9.1 SYSMEX KX 21 QUICK GUIDE 9.2 OPERATORS MANUAL FOR AUTOMATED ANALYZER KX 21 by SYSMEX CORPORATION 1997-2004

Note: Safety precaution: When analyzing samples, always wear rubber gloves and adhere to standard safety procedures.

END OF DOCUMENT
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