PSORT OF QUERY SEQUENCE

Input Sequence
QUERY (390 aa) MEEPGAQCAP LLVMLLALIT LVMPISTMYT ITDAVEYSAK VNTDHILYTV LTRAQLITDS KKKLMAARER FDFFTWLGYL PPPAGSETWV LATTLSNAFV VTGRWTLGQV RTPKRAAVMI YSTVGAFYFP PGSTSSVTSI KATKTLGIIL NSLINPIIYT PQANLSSAPS IATVYRTRKL VCDFWLSSDI ALVWVFSISI TLLLIALYGR NSRVPDVPSE GAFIVCWLPF MSNEDFKQAF QNCSAKDYIY HTPANYLIAS TCCTASILHL SLPPFFWRQA IYVEARSRIL SGSPVYVNQV FIISLVMPIC HKLIRFKCTS QDSISLPWKV LAVTDLLVSI CVIALDRYWA KAEEEVSECV KQTPNRTGKR KVRVSDALLE KDACWFHLAI

Results of Subprograms
PSG: a new signal peptide prediction method N-region: length 3; pos.chg 0; neg.chg 2 H-region: length 13; peak value 0.00 PSG score: -4.40 von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -2.37 possible cleavage site: between 61 and 62

GvH:

>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: INTEGRAL Likelihood = -8.07 Transmembrane 50 INTEGRAL Likelihood = -7.11 Transmembrane 87 INTEGRAL Likelihood = -2.34 Transmembrane 130 INTEGRAL Likelihood = -7.22 Transmembrane 166 INTEGRAL Likelihood = 0.10 Transmembrane 213 INTEGRAL Likelihood = -8.92 Transmembrane 317 PERIPHERAL Likelihood = 1.64 (at 335) ALOM score: -8.92 (number of TMSs: 6) MTOP: Prediction of membrane topology (Hartmann et al.) Center position for calculation: 57 Charge difference: 3.5 C( 3.5) - N( 0.0) C > N: C-terminal side will be inside >>> membrane topology: type 3b MITDISC: discrimination of mitochondrial targeting seq R content: 0 Hyd Moment(75): 7.66 Hyd Moment(95): 7.28 G content: 0 D/E content: 2 S/T content: 0

66 103 146 182 229 333

Score: -6.70 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: none content of basic residues: 9.2% NLS Score: -0.47 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: KKXX-like motif in the C-terminus: FKCT SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none

VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none none none

checking 71 PROSITE ribosomal protein motifs:

checking 33 PROSITE prokaryotic DNA binding motifs:

NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 94.1 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues

Results of the k-NN Prediction

k = 9/23
47.8 30.4 8.7 4.3 4.3 4.3 %: %: %: %: %: %: plasma membrane endoplasmic reticulum vacuolar Golgi mitochondrial nuclear

>> prediction for QUERY is pla (k=23)