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Versatility in a Complete Package ELISA Kits

Explore our broad range of versatile ELISA kits and obtain a sensitive, specific, quantitative assessment of diverse disease states including powerful biomarker assays for inflammation, metabolic syndrome and neuroscience.

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany


Neuropeptide & Neurodegenerative ELISA Kits

Understanding complex, interconnected nervous system functions and dysfunctions requires reliable quantification of biomarkers. Trust EMD Millipores neuropeptide and neurodegenerative ELISA kits to precisely quantify soluble biomarkers in sera and lysates. EMD Millipore is the leader in developing neural-specific ELISAs. We offer a growing

Discover the most reliable ELISAs for your research needs.

EMD Millipore has been the company of choice for single protein detection for many years. By choosing our ELISA (Enzyme-linked Immunosorbent Assay) kits, youll add 25+ years of experience in metabolic, neuroscience and cytokine* research. Our ELISA kits are developed, manufactured and shipped under stringent quality regulations. We understand that many research projects last months, even years, and you need to know that each kit will produce the same results, lot after lot. You also told us you wanted key metabolic assays to match the World Health Organization (WHO)** standards, and we listened! Several kits now are developed and tested based on prestigious WHO values. Along with smaller and more ecologicallyfriendly packaging, we are sure youll find EMD Millipores ELISA portfolio exceeds your research expectations.

Other enhanced features include:

Improved sensitivities for most panels 10-kit bulk/space saver options available Consistent kit and component storage conditions

* Human and Mouse Cytokine Assays coming soon. ** World Health Organization standards are available and used for final quality control testing.

Cytokine / Chemokine ELISAs


Cytokine / Chemokine ELISA Kits

Cytokine is a general term used for a diverse group of soluble proteins and peptides which act as regulators, under both normal and pathological conditions, to modulate the functional activities of individual cells and tissues. EMD Millipore is pleased to announce the upcoming launch of cytokine/chemokine ELISAs. These high-quality, cost-effective kits provide consistent and reliable measurement for all of your research needs.

Circulating Biomarkers
Description IL-2 IL-2 IL-4 IL-4 IL-6 IL-6 IL-10 IL-10 TNF- TNF- Species Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Catalogue No. Coming Soon Coming Soon Coming Soon Coming Soon Coming Soon Coming Soon Coming Soon Coming Soon Coming Soon Coming Soon

Neuroscience: Neuropeptide & Neurodegenerative ELISA Kits

Understanding complex, interconnected nervous system functions and dysfunctions requires reliable quantification of biomarkers. Trust EMD Millipores neuropeptide and neurodegenerative ELISA kits to precisely quantify soluble biomarkers in sera and lysates. As the leader in developing neural-specific ELISAs, we offer a growing selection of ELISAs supporting neuroscience research.

Description -Synuclein Amyloid b, 1-40 Amyloid b, 1-42 Amyloid b, Set Amyloid b (Brain), 1-40 Amyloid b (Brain), 1-42 Amyloid b (Brain), Set Chemikine Nerve Growth Factor, Sandwich Chemikine Brain Derived Neurotrophic Factor, Sandwich Chemikine Pigment Epithelium Derived Factor, Sandwich GFAP Nerve Growth Factor Neuropeptide S Neuropeptide Y Neuropeptide Y OxyELISA Oxidized Protein Quantitation Phosphorylated Neurofilament, (pNF-H) Sandwich S100B SAA-3 sICAM-1 sVCAM-1 Species Human, Mouse, Rat Human Human Human Human Human Human Mouse, Rat Human, Rat Human Human, Mouse, Rat Mouse, Rat Human Human Mouse, Rat Multi-species Multi-species Human Mouse Human Human Standard Curve Range 360 ng/mL 16500 pg/mL 16500 pg/mL 16500 pg/mL 16500 pg/mL 101000 pg/mL 7.8500 pg/mL 0.962.5 ng/mL 1.5-100 ng/mL 101000 pg/mL 39-5000 pg /mL 5-1000 pg /mL 0.01-2 ng /mL 07.2 nmol 0.029315 ng/mL 2.7-2000 pg /mL 0.078-5 g /mL 6.25-100 ng/mL 3.2100 ng/mL Sensitivity 3 ng/mL 4 pg/mL 5 pg/mL 4 pg/mL 5 pg/mL 10-15 pg/mL 7.8 pg/mL 0.9 ng/mL 1.5 ng/mL 10-15 pg/mL 20 pg/mL 2 pg /mL 0.004 ng /mL 0.2 nmol 0.0585 ng/mL 1.3 pg/mL 0.078 g/mL 2.17 ng /mL 0.59 ng/mL Sample Volume 100 L 50 L 50 L 50 L 50 L 50 L 50 L 50 L 100uL 50 L 50 L 50 L 20 L 100 L 1-10 L 50 L 10 L 10 L 10 L Catalogue No. NS400 EZHS40 EZHS42 EZHS-SET EZBRAIN40 EZBRAIN42 EZBRAIN-SET CYT304 CYT306 CYT420 NS830 CYT304 EZHNPS-34K EZHNPY-25K EZRMNPY-27K S7250 NS170 EZHS100B-33K EZMSAA3-12K ECM335 ECM340

Contains 1 each of EZHS40 and EZHS42

Contains 1 each of EZBRAIN40 and EZBRAIN42

Visualize and quantify neuroscience with validated neural marker antibodies.

By integrating the antibody expertise and resources of Calbiochem, Chemicon, Upstate, and Linco, EMD Millipore is now the leading provider of neuroscience antibodies for the central and peripheral nervous systems. Drive your neuroscience research forward with our vast portfolio of pathway-, cell type-, and state-specific antibodies and neurological disease assays and markers.

Neuroscience ELISAs

S100B (high)

Figure 1. Astrocyte
Mechanisms by which S100B can promote neurodegeration. When high levels of S100B are secreted by astrocytes, neurodegeneration can result, either by direct activation of reactive oxygen species (ROS) production and oxidative stress, or by indirect stimulation of microglia, causing secretion of proapoptotic cytokines. (Image adapted from Sorci G. et al., Cardiovascular Serum FGF-23 Levels Psychiatry and Neurology, 2010; in LPS Challenged Rats (n=3) vol. 2010, Article ID 656481, 13 pages. doi:10.1155/2010/656481).

Microglia activation
NO IL-1a TNF-a

IL-6 TNF-a NO TNF-a S100B (high)

Plasma FGF-23 Levels in LPS Challenged Mice (n=4)

Mouse FGF-23 (ng/mL)

2 1.5 1 0.5 0 No LPS 1 hr. LPS 2 hr. LPS 4 hr. LPS 8 hr. LPS

Neuron ROS production, death

Rat FGF-23 (ng/mL)

3.5 3 2.5 2 1.5 1 0.5 0

0 hrs.

2 hrs.

4 hrs.

8 hrs.

Concentration of S100B in Human Plasma (pg/mL)

120.00 100.00 80.00 60.00 40.00 20.00 0

Control Schizophrenia

Figure 2.
Plasma from SCZ patients shows higher expression levels of S100B compared to control subjects. S100B was measured in plasma using the Human S100B ELISA (Cat. No. EZHS100B-33K). The sample set was split into two groups (control n=81, SCZ n=80). A non-parametric test (Mann-Whitney) was applied to compare mean values between both groups. A significant difference is observed in the mean values between control and the SCZ group (p=0.003, error bars indicate the standard error of the mean).

To download the white paper, S100B as a Biomarker for Ca2+ Signaling Dysregulation in Schizophrenia, visit:

Metabolic/Endocrine ELISAs
Measuring the concentrations of circulating biomarkers can help elucidate therapeutic mechanisms of action, establish early diagnosis of disease, predict toxicity, and more. Our broad range of ELISA kits provide a comprehensive assessment, particularly when studying metabolic disease.

Circulating Biomarkers
Description Adiponectin Adiponectin Adiponectin Adiponectin Adiponectin-High Molecular Weight Adipsin Angiopoietin-related Growth Factor (AGF) / ANGPTL6 Amylin (active) Amylin (total) APO AIV APO AV BDNF Chemerin C-Peptide C-Peptide 2 CRP CRP CRP FGF-21 FGF-21 FGF-23 FGF-23 FGF-23 Fibronectin (Quantimatrix) Ghrelin (active) Ghrelin (active) Ghrelin (total) Ghrelin (total) GIP (total) GIP (total) GLP-1 (active) GLP-1 High Sensitivity (active) a Species Human Mouse Rat Canine Human Mouse Human Standard Curve Range 1.56200 ng/mL 150 ng/mL 3.125200 ng/mL 0.137100 ng/mL 1.56200 ng/mL 0.0392.5 g/mL 31-2000 ng/mL Sensitivity 0.2 ng/mL 0.2 ng/mL 0.4 ng/mL 0.03 ng/mL 0.2 ng/mL 0.01 g/mL 5.0 ng/mL Sample Volume 10 L 10 L 10 L 10 L 20 L 20 L 10 L Catalogue No. EZHADP-61K EZMADP-60K EZRADP-62K EZCADP63K EZHMWA-64K EZMADPSN-22K EZHAGF-28K EZHMWA-64BK Bulk Packaging Catalogue No* EZHADP-61BK EZMADP-60BK

Human Human Human Human Human, Rat Human Human Mouse, Rat Canine Rat Human Human Mouse, Rat Human Mouse Rat Human Human Mouse, Rat Human Mouse, Rat Human Mouse, Rat Multispecies Multispecies

1100 pM 1100 pM .0785 g/mL 4.96300 ng/mL 7.8500 pg/mL 1.56100 ng/mL 0.220 ng/mL 251600 pM 1.540 ng/mL 4.9133.3 g/mL 0.1210 ng/mL 31.252000 pg/mL 49.412,000 pg/mL 9.92400 pg/mL 0.137 - 100 ng/mL 31000 ng/mL 252000 pg/mL 252000 pg/mL 1005000 pg/mL 0.110 ng/mL 8.22000 pg/mL 8.22000 pg/mL 2100 pM

0.7 pM 0.6 pM 0.02 g/mL 1.1 ng/mL 7.8 pg/mL 0.5 ng/mL 0.05 ng/mL 15.0 pM 3.6 ng/mL 2.5 ng/mL 0.2 ng/mL 10.0 pg/mL 10.0 pg/mL 3.5 pg/mL 0.69 pg/mL 10-20 ng/mL 15.0 pg/mL 8.0 pg/mL 50.0 pg/mL 0.04 ng/mL 4.2 pg/mL 4.2 pg/mL 1.0 pM

50 L 50 L 5 L 20 L 50 L 50 L 10 L 20 L 2 L 2 L 2 L 50 L 10 L 50 L 20 L 50 L 100 L 20 L 20 L 20 L 20 L 20 L 10 L 100 L





WHO Standard values matched to the World Health Organization guidelines. * Bulk packaging now available on select kits more environmentally friendly and saves space. ** Available in May, 2012. a chemiluminescent assay

Metabolic / Endocrine ELISAs

Circulating Biomarkers (continued)

Description GLP-1 (total) GLP-2

Species Multispecies Multispecies Human, mouse, Rat Mouse, Rat Human Human Human Rat, Mouse Human Human Canine Mouse Rat Human Human Human Human

Standard Curve Range 4.1-1000pM 1-64ng/mL

Sensitivity 1.0 pM 0.3 ng/mL

Sample Volume 20 - 50 L 50 L

Catalogue No. EZGLP1T-36K EZGLP2-37K Coming Soon

Bulk Packaging Catalogue No* EZGLP1T-36BK

Glucagon a Growth Hormone ICAM-3 IFNa

Coming Soon EZRMGH-45BK

0.750 ng/mL 0.7850 ng/mL 12.5500 pg/mL 2200 U/mL 0.210 ng/mL 2200 U/mL 103000 ng/mL 0.7850 ng/mL 0.230 ng/mL 0.230 ng/mL 0.5100 ng/mL 2-200 ng.mL 12.63000 pg/mL lot dependent

0.07 ng/mL 0.58 ng/mL 12.5 pg/mL 1.0 U/mL 0.1 ng/mL 0.85 U/m 30-70 ng/mL 0.21 ng/mL 0.05 ng/mL 0.08 ng/mL 0.2 ng/mL 0.23ng/mL 12.3 pg/mL 30.0 ng/mL

10 L 20 L 100 L 20 L 10 L 20 L 100 L 20 L 10 L 10 L 25 L 20 L 50 L 5 L



Insulin Insulin Insulin (Animal serum free) Laminin Leptin Leptin Leptin




Leptin "Dual Range" Omentin-1 Pancreatic Polypeptide Procollagen Type IIA N-Propeptide (PIIANP)



Proinsulin (intact) Proinsulin (total) PYY (total) RBP4 Resistin sCD26 sE-Selectin

Human Human Human Human Human Human Human

0.5100 pM 2200 pM 102000 pg/mL 0.14100 ng/mL 0.165 ng/mL 15.6500 ng/mL 0.850 ng/mL

0.2 pM 0.5 pM 6.5 pg/mL 0.07 ng/mL 0.02 ng/mL 7.26 ng/mL 0.33 ng/mL

50 L 20 L 20 L 10 L 20 L 20 L 20 L


Plasma FGF-23 Levels inin Plasma FGF-23 Levels LPS Challenged Mice (n=4) LPS Challenged Mice (n=4)
Mouse FGF-23 (ng/mL) Mouse FGF-23 (ng/mL)

Serum FGF-23 Levels Serum FGF-23 Levels inin LPS Challenged Rats (n=3) LPS Challenged Rats (n=3)
Rat FGF-23 (ng/mL) Rat FGF-23 (ng/mL)
3.5 3.5 33 2.5 2.5 22 1.5 1.5 11 0.5 0.5 00 0 hrs. 0 hrs. 2 hrs. 2 hrs. 4 hrs. 4 hrs. 8 hrs. 8 hrs.

2.5 2.5 22

1.5 1.5 11

0.5 0.5 00 No LPS 1 LPS LPS LPS8 LPS No LPS 1 LPS 2 2 LPS 4 4 LPS 8 LPS

f S100B in Human Plasma (pg/mL) f S100B in Human Plasma (pg/mL)

120.00 120.00 Figure 3A.

Figure 3B.
The serum samples were collected after LPS injection (1mg/1 kg body weight) at 0, 2, 4 and 8 hours, and measured with Rat FGF-23 ELISA.

The plasma samples were collected after LPS injec100.00 tion100.00 kg body weight) at 1, 2, 4, 8 hours, and (1 mg/1 measured with Mouse FGF-23 ELISA. 80.00 80.00
60.00 60.00 40.00 40.00

Cellular Metabolism Activity Assays & ELISAs

Study how the cell generates energy using our new Cellular Metabolism Activity Assays and ELISAs. These kits allow you to examine protein levels and activity and to expand your research into areas such as mitochondrial dysfunction, toxicity, metabolic disorders, neurological disorders, cancer and aging.
Description Mitochondrial Complex I Activity Assay Kit Mitochondrial Complex II Activity Assay Kit Mitochondrial Complex IV (Human) Activity Assay Kit Mitochondrial Complex V Activity Assay Kit Mitochondrial Complex IV (Mouse) Activity Assay Kit PDH Activity Assay Kit Aconitase Activity Assay Kit Cytochrome c ELISA kit Frataxin ELISA Kit PDH ELISA Kit Species human, mouse, rat human, mouse, rat, bovine human, bovine human, rat, bovine mouse, rat human, mouse, rat, bovine human, mouse, rat, bovine human, mouse, rat, bovine human human, mouse, rat, bovine Catalogue No. AAMT001-1KIT AAMT002-1KIT AAMT004-1KIT AAMT005-1KIT AAMT006-1KIT AAMT008-1KIT AAMT009-1KIT EAMT001-1KIT EAMT002-1KIT EAMT003-1KIT

Cell Signaling ELISAs

Determining how the elements of signal transduction interact both within and between pathways is essential to understanding normal and disease processes. Choose among our ELISAs detecting phosphorylation states of key signaling proteins, second messengers transmitting intracellular signals and even apoptosis.

Description Akt1 EGFR ErbB2 (Tyr1248) ERK 1/2 FAK Insulin Receptor (b subunit) IkB JNK 1/2 MEK1 Met p38 Phospho-Akt (Ser473) Phospho-Akt (Thr308) Phospho-EGFR (Tyr1068) Phospho-EGFR (Tyr1173) Catalogue No. 17-455 17-460 17-490 17-463 17-479 17-483 17-485 17-465 17-473 17-469 17-487 17-457 17-456 17-462 17-461 Description Phospho-FAK (Tyr397) Phospho-IR (b subunit) (Tyr 1162/1163) Phospho-IRS1 (Ser307 mouse/Ser312 human) Phospho-IkB (Ser32) Phospho-JNK 1/2 (Thr183/Tyr185) Phospho-MEK1 (Ser218/222) Phospho-MET (Tyr1230/Tyr1234/ Tyr1235) Phospho-p38 (Thr180/Tyr182) Phospho-p53 (Ser15) Phospho-PRAS40 (Thr246) PRAS40 Catalogue No. 17-480 17-484 17-459 17-486 17-466 17-474 17-470 17-488 17-475 17-478 17-477

Other ELISAs

Transcription Factor Assay (TFA) ELISAs

Quickly and accurately determine the effect of cell signaling on gene expression with EZ-TFA nonradioactive transcription assays. These ELISAs detect the DNA binding activity of transcription factors in whole cell or nuclear extracts in a convenient 96-well format. With a choice of colorimetric or ultra-sensitive chemiluminescence detection, these assays combine the principles of the electrophoretic motility shift assay (EMSA) and gel super-shift assays with the 96-well based enzyme-linked immunosorbent assay (ELISA), providing greater sensitivity than conventional EMSA assays.

EZ-Transcription Factor
Description AP-1 Family c-Fos c-Jun ErbB2 (Tyr1248) HDAC Jun/Fos NFkB Family NFkB p50 NFkB p50/p65 NFkB p65 PhosphaFluor HTS OmniPhosphatase Ras Activation ssDNA Apoptosis Universal TF Colorimetric Catalogue No. 70-550 70-545 70-540 17-490 17-374 70-546 70-560 70-515 70-510 70-520 17-448 17-497 APT 225 70-501 70-601 70-646 70-660 70-615 70-610 70-620 70-645 70-640 Chemiluminescent Catalogue No.

Cell-Based ELISAs
Measure your proteins of interest in their native context using whole cell ELISAs instead of cell lysates. Our whole cell-based ELISAs include Duel Detect CELISAs assays to measure intracellular signaling by both total and phosphorylated targets in whole cells.

Dual Detect CELISAs

Description ErbB2 (Tyr1248) H2A.X (Ser139) Catalogue No. 17-490 17-720

Extracellular Matrix (ECM)

ECM is a complex structural entity that surrounds and supports the cells within living systems and actively regulates a diverse range of cell functions. These circulating adhesion molecules can be easily measured with the following selection of ELISA kits.

Description Fibronectin sCD26 sE-Selectin sICAM-1 sVCAM-1 Species Human Human Human Human Human Catalogue No. ECM300 ECM345 ECM330 ECM335 ECM340

Radioimmunoassays (RIAs)
RIAs have long been considered a valuable, economical and accurate way to measure protein concentration. And, RIA data is frequently used as a benchmark for validation of alternative protein quantification methods. EMD Millipores RIA kits have long been considered the gold-standard for many common metabolic biomarkers.

Description Adiponectin Adiponectin C-Peptide C-Peptide C-Peptide C-Peptide Ghrelin (active) Ghrelin (total) GLP-1 (active) GLP-1 (total) Glucagon Insulin Insulin Insulin LisPro Insulin (sensitive) Insulin Specific Insulin Ultra Sensitive Leptin Leptin Leptin Leptin Leptin (sensitive) Proinsulin PYY PYY (3-36) PYY (total) Species Human Mouse Canine Human Rat Porcine Human Human Multi-species Multi-species Multi-species Porcine Rat Multi-species Rat Human Human Human Multi-species Mouse Rat Human Human Mouse, Rat Human Human Standard Curve Range lot dependent 0.78100 ng/mL 0.15620 ng/mL 0.15 ng/mL 2521600 pM 0.16-5ng/mL lot dependent lot dependent 10500 pM 101000 pM 20400 pg/mL 2200 U/mL 0.110 ng/mL 2.5250 U/mL 0.021 ng/mL 2-200 U/mL 0.2-20 U/mL 0.5100 ng/mL 150 ng/mL 0.220 ng/mL 0.550 ng/mL 0.0510 ng/mL 2100 pM lot dependent lot dependent lot dependent Sensitivity 1 ng/mL 1 ng/mL 0.15 ng/mL 0.1 ng/mL 25 pM 0.1ng/mL 7.8 pg/mL 93 pg/mL 3pM 3pM 20 pg/mL 2 U/mL 0.1 ng/mL 2.5 U/mL 0.02 ng/mL 2 U/mL .02 U/mL 0.5 ng/mL 1 ng/mL 0.2 ng/mL 0.5 ng/mL 0.05 ng/mL 2pM 15.6 pg/mL (78.1 pg/mL) 20 pg/mL 10 pg/mL Sample Volume 5 L 5 L 50 L 50 L 50 L 50uL 50 L 50 L 300 L 300 L 50 L 50 L 50 L 100 L 50 L 50 L 10 L 50-100 L 50 L 50 L 50 L 50 L 100-200 L 100 L (20 L) <100 L <100 L Catalogue No. HADP-61HK MADP-60HK CCP-24HK HCP-20K RCP-21K PCP-22K GHRA-88HK GHRT-89HK GLP1A-35HK GLP1T-36HK GL-32K PI-12K RI-13K LPI-16K SRI-13K HI-14K HI-11K HL-81K XL-85K ML-82K RL-83K SHL-81K HPI-15K RMPYY-68HK PYY-67HK PYYT-66HK

RIAs / Services

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EMD Millipore, the M mark and Dual Detect CELISA are trademarks of Merck KGaA, Darmstadt, Germany. Calbiochem, Chemicon, Upstate, Linco, and OxyELISA is a registered trademark of Merck KGaA, Darmstadt, Germany. Lit. No. DS3425EN00, Rev. B LS-SBU-12-06044 3/12 2012 Millipore Corporation, Billerica MA, 01821 USA. All rights reserved.