Mobile phase
Conc. 1 10mM
PIC B-5
Packing: Column: Solvent: Bondapak C18 4 mm ID x 30cm Methanol/H2O with 0.005M PENTANE Sulfonic Acid & 1% HOAc (50/50) Flow Rate: 2.0 ml/min Detector: UV, 254 nm, 0.1 AUFS 1. Maleic Acid 2. Phenylephrine 3. Phenylpropanolamine 4. Naphazoline 5. Phenacetin 6. Pyrilamine
PIC B-6
Packing: Column: Solvent: Bondapak C18 4 mm ID x 30cm Methanol/Water with 0.005M HEXANE Sulfonic Acid & 1% HOAc (50/50) Flow Rate: 2.0 ml/min Detector: UV, 254 nm, 0.1 AUFS 1. Maleic Acid 2. Phenylephrine-HCl 3. Phenylpropanolamine-HCl 4. Naphazoline-HCl 5. Phenacetin 6. Pyrilamine Maleate
PIC B-7
Flow Rate: 2.0 ml/min Detector: UV, 254 nm, 0.1 AUFS
Paired-Ion Chromatography
Water Soluble Vitamins
Column: Bondapak C18 4 mm ID x 30 cm Solvent MeOH: H2O, PIC B-5 Sample: Water Soluble Vitamins
Paired-Ion Chromatography
Water Soluble Vitamins
Column: Bondapak C18 4 mm ID x 30 cm Solvent: MeOH:H2O, PIC B-5 & B-7, 50:50 Sample: Water Soluble Vitamins 1) 2) 3) 4) Niacinamide Pyridamine Riboflavin Thiamine
+ H3N
+ H3N OH COOH
COO H
H3N + O O C F 3
-+ COO H OH
-+ COO H
Compounds: 1. L-ascorbic acid 2. Nicotinic acid 3. Thiamine 4. Pyridoxal 5. Pyridoxine 6. Folic acid 7. Caffeine 8. Riboflavin
USP Tailing 1.12 1.27 1.20 1.13 1.04 1.10 1.10 1.14
V0 = 1.37 min
4 5 6
1.00
2.00
3.00
4.00
5.00
6.00
9.00
10.00
11.00
12.00
13.00
14.00
15.00
Conditions Column: PolarityTM 4.6 x 150 mm, 5 m Mobile Phase: 88% A; 2% B; 10% C Flow Rate: 1.0 mL/min A: H2O B: ACN C: 100 mM CH3COONH4, pH 5.0 Injection Volume: 10.0 L Temperature: 30 oC Detection: UV @ 280 nm
Compounds USP Tailing 1. Norepinephrine (NE) 1.21 2. Epinephrine (E) 1.20 3. Dopamine (DA) 1.21 4. 3,4-Dihydroxyphenylacetic acid (DOPAC) 1.00 5. Serotonin (5-HT) 1.10 6. 5-Hydroxy-3-indoleacetic acid (5-HIAA) 0.97 7. 4-Hydroxy-3-methoxyphenylacetic acid (HVA) 0.97
2 1 3
V0 = 1.83 min
2.00
4.00
6.00
8.00
10.00
12.00
18.00
20.00
22.00
24.00
26.00
28.00
30.00