BASI C

PRA CTI CAL

MI CRO BI

OLO GY: A

MAN UAL

 The Society for General

Microbi ology 2001

Con t ent

Part 1: THE

BASI CS

An intro ducti

on to micr obiol

ogy, asep tic

tech niqu e

and safet y

Pag e

Num ber

PRE PAR

ATI ON

Saf ety

guide lines 1

Ris k asses

smen t 1

Goo d micro

biolo gical labor

atory practi ce

(GML P) 3

Spil lage mana

geme nt 3

Aer osols 4

RES OUR CES

Equ ipme nt

Ap parat us

Ma terial s

MED I A,

STE RI LI ZATI

ON AND DI SI

NFE CTA NTS

Pre parati on of

cultur e

medi a 6

Sto rage of

medi a 6

Ster ilizati onvs.

disinf ectio n

6
Use of the

autoc lave/ press

ure cooke r

6
Ster ilizati

on of equip ment

and mater ials

7 Ch oice,

prepa ratio n and

use of disinf

ectan ts 7

I NOC ULA

TI ON AND

OTH ER ASE

PTI C PRO

CED URE S

Ess ential

point s 8

Usi ng a

wire loop 8

Usi ng a

pipett e 10

Fla ming the

neck of bottle

s and test tubes

10

Stre ak plate

12

Pou r plate

13 Usi ng a

sprea der 15

Spr ead plate

16

I NCU

BATI ON
17

IN CON CLU

SI ON: CLE

ARI NG UP

17

BASI C

PRA CTI CAL

MI CRO BI

OLO GY: A

MAN UAL

 The Society for General

Microbi ology 2001

Part 2:MI CRO

BI OLO GYI

NAC TI ON

Esse ntial meth

ods form ainta

ining ,prep aring

and usin g

cultu res

Pag e

Num ber

MAI NTE NAN

CE, PRE PAR

ATI ON AND

SUB CUL

TUR I NG

Obt ainin g

suita ble

cultur es 18

Pur e

cultur es 18

Mai ntaini ng

stock cultur es

18 Che cking

cultur es for conta

minat ion 18

Pre venti ng

conta minat ion of

cultur es and

the envir

onme nt 19

Ase ptic transf

er of cultur es

and steril e

soluti ons 19

Ap propr iate

instru ment s for

asepti c transf

er proce dures

19 Pre parin

g cultur es of

bacte ria and

yeast s for

class use 20

Pre parin g

cultur es of moul

ds for class use

20

TW O

ACTI VI TI ES

1. Testi ng

sensit ivity to

antib acteri al

subst ances 21

2. Micro scopy

= Usi

ng the

micro scope 22

= Sta

ined prepa

ratio ns 22

= Ma

king a

smea r 23

= A

simpl

e stain 23

= A

differ ential

stain: Gram s

staini ng

meth od 24

Facts heets to

supp ort this

manu al can be

down loade d

from the www.

micro biolo gyonl

ine.or g.uk

websi te.

ACK NOW LED

GEM ENTS

The Socie ty for

Gene ral Micro

biolo gy is gratef

ul to ackno wledg

e the supp ort

from the follo

wing sourc es:

= Kat

h Crawf

ord (SAP S

Scotl and) = Joh

n Richa rdson

(SSE RC) = Joh

n Trant er

(CLE APSS Scho

ol Scien ce

Servi ce) = Me

mber s of

MISA C

BASI C PRA

CTI CAL MI

CRO BI OLO

GY: A

MAN UAL

 The Society for General

Microbi ology 2001

BA SI C

PR AC

TI CAL

MI CR

OBI OL

OG Y

Part 1: THE

BASI CS

An intro ducti

on to micr obiol

ogy, asep tic

tech niqu e

and safet y

As well as

causi ng a famil

iar rang e of

disea ses in

anim als and

plant s and probl

ems in food

spoil age and

deter iorati on of

other mate rials,

micr obes are

also our invi

sible allies .

Inde ed, life

on Eart h

woul d not be

susta inabl e

with out the

bene fits that

man y of them

provi de. The

teach ing of

such an impo

rtant subje ct

as micr obiol

ogy cann ot be

achie ved effect

ively with out

enha ncing the

theor y with

han ds on

expe rienc e

in the labor

atory . The purp

ose of this

man ual is to

provi de teach

ers and tech

nicia ns with

good tech niqu

es in pract

ical micr obiol

ogy to ensu

re that inves

tigati ons proc

eed safel y and

achie ve the

requi red educ

ation al

aims succe

ssfull y.

PRE PAR

ATI ON

Saf et ygui

delin es

The small size

of micr obes

and the cons

eque nt need

to deal with

cultu res that

conta in man

y milli

ons of

micr obial cells

requi re speci

al proc edur

es for their safe

use. Activ ities

invol ving micr

oorga

nism s are

contr olled by

the C ontrol of

Subst ances H

azard ous to H

ealth (COS HH)

Regu lat ion

s and teach ers

and tech

nicia ns

have a duty

unde r the H

ealth and Safety

at W orkA ct to

com ply with

any safet y

instr uctio ns

given by their

empl oyers .

Thes e inclu

de using mod

el risk asses

smen ts for whic

h it is nece ssary

to refer to

appr opria tepu

blica tions such

as To pics in

Safety , 3rd ed

ition (ASE ,

2001 ), Micro

biolo gy: an

HMI Guide (DE

S, 1990 ) and