2 BIOLOGICAL MEMBRANES
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3.2.2 Fluid Mosaic Model
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Hydrophilic region
of protein
Phospholipid
bilayer
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• Plasma membrane is a continuous, fluid,
double layer of phospholipids, the lipid
bilayer.
• Phospholipids & most other membrane
constituents are amphipathic molecules -
have hydrophobic regions & hydrophilic
regions.
Hydrophobic tails face inside of bilayer.
Hydrophilic head faces exterior
(extracellular fluid) and interior
(cytosol).
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WATER
Hydrophilic
head
Hydrophobic
tail
WATER
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• Proteins - embedded in bilayer or
associated with cytoplasmic or
extracellular face.
• Carbohydrates - linked to proteins
(glycoproteins) or lipids (glycolipids)
only on extracellular side.
• Cholesterol - lies within membrane.
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• Membrane molecules held in
place by weak hydrophobic
interactions.
• Most lipids & some proteins drift
laterally.
• Rarely flip-flop from one layer to
the other.
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Lateral movement Flip-flop
(~107 times per second) (~ once per month)
Movement of phospholipids
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• Many larger membrane proteins drift
within the phospholipid bilayer.
• Proteins are much larger than lipids
and move more slowly.
• Other proteins are anchored to
cytoskeleton.
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3.2.3 Membrane Components
a) Membrane proteins
• Amphipathic.
• Determine most of membrane’s
specific functions
• Two groups:
i. Peripheral proteins
Not embedded but loosely bound to
surface of protein.
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Fibers of
extracellular
matrix (ECM)
Glycoprotein
Carbohydrate
Glycolipid
EXTRACELLULAR
SIDE OF
MEMBRANE
Cholesterol
Microfilaments Peripheral
of cytoskeleton proteins Integral
protein
CYTOPLASMIC SIDE
OF MEMBRANE
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ii. Integral proteins
Penetrate hydrophobic core, often
completely as transmembrane
proteins.
proteins.
Hydrophobic segments consist of
stretches of non-
non-polar amino acids,
coiled into α-helices.
Hydrophilic segments have
hydrophilic non-
non-helical amino
acids.
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EXTRACELLULAR
SIDE
N-terminus
C-terminus
CYTOPLASMIC
SIDE
α Helix
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Six major functions of protein
1. Transport
2. Enzymatic activity
3. Signal transduction
4. Cell-cell recognition
5. Intercellular joining
6. Attachment to the cytoskeleton
and extracellular matrix (ECM)
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Signal
Enzymes
Receptor
ATP
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Glyco-
protein
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(b) Carbohydrates
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(c) Cholesterol
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3.3 Movement Across Membranes
• Hydrophobic molecules
(hydrocarbons, CO2, & O2) dissolve in
lipid bilayer & cross easily.
• Hydrophobic core of membrane
impedes passage of ions and polar
molecules (water, glucose & other
sugars) - cross membrane with
difficulty.
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• Ions and polar molecules cross bilayer
through transport proteins:
proteins
i. Channel proteins:
proteins Have hydrophilic
channel for passage of certain
molecules or ions.
Example, passage of water
through membrane facilitated by
channel proteins known as
aquaporins.
aquaporins.
ii. Carrier proteins:
proteins Bind to molecules
& change shape to shuttle them
across membrane.
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3.3.1 Passive Transport
a) Diffusion
Spontaneous tendency of molecules
of any substance to move down its
concentration gradient from a more
concentrated to a less concentrated
area.
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• Individual molecule moves randomly.
• But diffusion of a population of
molecules exhibit a net movement in
one direction.
• At dynamic equilibrium, as many
molecules cross one way as cross in
the other direction.
• Each substance diffuses independent
of the concentration gradients of other
substances.
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Net diffusion Net diffusion Equilibrium
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• No work done to move substances
down concentration gradient.
• Diffusion of substance across
biological membrane is passive
transport - requires no energy from the
cell.
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Factors determining rate of
diffusion
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• The rate of diffusion is directly proportional to
the concentration difference and surface
area, and inversely proportional to the
diffusion distance.
Surface x Concentration
Rate of diffusion ∝ area distance
Diffusion distance
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• Diffusion through membrane (barrier)
affected by:
i. Nature of membrane, for example,
its permeability.
ii. Size and type of molecule or ion
diffusing through it.
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b) Osmosis
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• Tendency of water molecules to move
across membrane depends on:
i. Solute concentration.
ii. Pressure on each side of membrane.
• Water potential (ψ
ψ) - combined effect
of solute concentration and pressure
in a solution.
Units in kilopascals (kPa)
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• Water potential of a solution is the
tendency for water to diffuse out of it.
• Water diffuses from a high water
potential to a low water potential,
down its water potential gradient.
• By definition, pure water at
atmospheric pressure has a water
potential of zero.
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Effect of concentration on water
potential and osmosis:
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Lower Higher Same concentration
concentration concentration of sugar
of solute (sugar) of sugar
H2O
Selectively
permeable mem-
brane: sugar mole-
cules cannot pass
through pores, but
water molecules can
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Increased pressure
There is a substantial
movement of water in
osmosis
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High water potential
Pressure applied
on this side
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• Effect of pressure on a solution is
called the pressure potential (Ψ Ψp).
• Value of Ψp usually positive.
In plants, this is the force of the cell
wall pushing inwards on contents of
cells (cytoplasm) when water enters
cell by osmosis.
In animals this may be due, for
example, to high blood pressure in
glomerulus of the kidney.
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Water potential = Solute potential + Pressure potential
ψ = ψS + ψP
(usually negative) (usually negative) (usually positive)
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Effect of osmosis on plant cells
• Plant cells generally have lower water
potential than that of their surroundings.
Due to presence of solutes in fluid
within vacuole (cell sap).
• Plasma membrane & tonoplast
surrounding vacuole are both partially
permeable, letting water through but not
solutes.
• Cell wall permeable to both water &
solutes.
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• Refer to handout for effect of osmosis
on plant cells.
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Effect of osmosis on animal cells
• Refer to handout.
• Animals without rigid cell walls have
osmotic problems in environment with
low or high water potential.
• To maintain their internal environment,
they must have adaptations for
osmoregulation.
• Paramecium, which has low water
potential compared to its pond water
environment, has a contractile vacuole
that acts as a pump.
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50 µm
Filling vacuole
50 µm
Contracting vacuole
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Worked examples on the water potential
concept
Example 1
• A plant cell with a water potential of –700 kPa
is immersed in a sucrose solution whose
water potential is –350 kPa. In which
direction will water flow?
Answer
• Water will flow from the sucrose solution into
the cell. This is because the water potential
of the cell is lower than (i.e. more negative
than) the sucrose solution, and there is a net
flow of water from a region of higher water
potential to a region of lower potential, i.e.,
down the water potential gradients.
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Worked examples on the water
potential concept
Example 2
• A plant cell has a solute potential of –240
kPa and a pressure potential of 350 kPa.
What is the water potential of the cell?
Answer
Water potential = Solute potential +
Pressure potential
(Ψ) = (Ψs) + (Ψp)
= -1240 + 350
= -890 kPa
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Worked examples on the water
potential concept
• Example 3:
• A plasmolysed cell is found to have a
solute potential of –960 kPa. What is
the water potential of the cell?
• Answer
• In a plasmolysed cell, pressure
potential (Ψp) is 0.
• Therefore, Ψ = Ψs = -960 kPa
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Worked examples on the water
potential concept
• Example 4:
• Two plant cells, A and B, are next to each
other in a tissue. The water potential of
cell A is –700 kPa, and the water potential
of cell B is –550 kPa. In which direction
will water flow – from A to B, or from B to
A?
• Answer
• Water will flow from B to A. This is
because water flows down a water
potential gradient.
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c) Facilitated Diffusion
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• Two types of transport proteins:
i. Channel proteins
Some provide hydrophilic corridors
for passage of specific molecules
or ions.
Example, aquaporins,, facilitate
diffusion of water.
Many ion channels function as
gated channels - open or close
depending on presence or absence
of a chemical or physical stimulus.
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EXTRACELLULAR
FLUID
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ii. Carrier proteins
Some proteins translocate the
solute-binding site and solute
across the membrane as the
transport protein changes shape.
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3.3.2 Active Transport – Sodium
Pump and Coupled Transport
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EXTRACELLULAR [Na+] high Na+
FLUID [K+] low Na+
Na+ Na+
Na+
K+
K+
K+
K+
K+
P
P K+
ATP
Diffusion Facilitated diffusion
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Maintenance of Membrane Potential by
Ion Pumps
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• Membrane potential favors passive
transport of cations into cell and
anions out of cell.
• Two combined forces, the
electrochemical gradient,, drive
diffusion of ions across a membrane.
1)Chemical force: an ion’s
concentration gradient.
2)Electrical force: effect of membrane
potential on ion’s movement.
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• Special transport proteins, the
electrogenic pumps,, generate voltage
gradient across a membrane.
• Example:
Sodium-potassium pump in animal
cells.
Proton pump in plants, fungi, &
bacteria.
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– EXTRACELLULAR
+
FLUID
ATP –
+ H+
H+
Proton pump
H+
– + H+
H+
– +
CYTOPLASM
H+
– +
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Cotransport
• The coupling of the diffusion of one
substance down its concentration
gradient to the transfer of another against
its concentration gradient.
• Transport protein may move two
substances in the:
Same direction – symport carriers.
Opposite directions – antiport carriers.
carriers
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• Plants commonly use the gradient of
hydrogen ions generated by proton pumps
to drive active transport of nutrients into
the cell.
– +
ATP H+
H+
– +
Proton pump H+
H+
– +
– H+
+
H+ Diffusion
Sucrose-H+ of H+
cotransporter
H+
– +
– + Sucrose
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3.3.3 Bulk Transport
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Exocytosis
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ER
Transmembrane
glycoproteins
Secretory
protein
Glycolipid
Golgi
apparatus
Vesicle
Plasma membrane:
Cytoplasmic face
Extracellular face
Transmembrane
Secreted glycoprotein
protein
Plasma membrane:
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Endocytosis
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Three types of endocytosis:
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RECEPTOR-MEDIATED ENDOCYTOSIS
Coat protein
Receptor Coated
vesicle
Coated
pit
Ligand
A coated pit
Coat and a coated
protein vesicle formed
during
receptor-
mediated
endocytosis
(TEMs).
Plasma
membrane
0.25 µm
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