Universal Science College

(B. Sc. Biochemistry)

Fourth Semester 2005
Internal Examination Full Marks: 100
Subject: Biochem 222 (Bioenergetics and Metabolism) Pass Marks: 45
Date: August 1, 2005 Time: 3 hours
Candidates are required to give their answers in their own words as far as practicable. The figure
in the margin indicates full marks.
Group (A) Long Questions (Any three) ×14 = 42)
(3×

1. Explain standard free energy and actual free energy. "Standard free energy changes are
additive." Explain and verify the statement.
2. What is EMP pathway for glucose metabolism? Explain the steps involved.
3. What is TCA cycle? Describe the various steps of TCA cycle.
4. Describe the process of electron transfer in mitochondra

Group (B) Short Questions: (Any six) ×7 = 42)

(6×
1. How does ATP transfer energy in metabolic process?
2. What are the universal electron carriers? Describe any two of them.
3. Describe pentose phosphate pathway.
4. What do you mean by anaplerotic reaction? List out the various anaplerotic reactions of TCA
cycle.
5. Describe the Glyoxylate Cycle.
6. What is chemiosmotic model for ATP synthesis?
7. How many molecules of ATP are released from the complete breakdown of a glucose molecule?
8. Write short note on ATP synthase complex.

Group (C) Write short notes on: (Any eight) ×8 = 16)

(2×
1. Define metabolism.
2. Enthalpy
3. Transphosphorylation
4. Flavin Nucleotides
5. Dihydrolipoyl transacetylase
6. Nucleoside diphosphate kinase
7. Fate of pyruvate under anaerobic condition in yeast.
8. Phosphofructokinase in regulation of glycolysis.
9. Lactose metabolism.
10. Amphibolic pathway
11. Isocitrate lyase
12. Cytochrome oxidase system.
***
 Universal Science College
(B. Sc. Biochemistry)
Fourth Semester 2005
Internal Examination Full Marks: 100
Subject: Biochem 222 (Bioenergetics and Metabolism) Pass Marks: 45
Date: August 1, 2005 Time: 3 hours
Candidates are required to give their answers in their own words as far as practicable. The figure
in the margin indicates full marks.
Group (A) Long Questions (Any three) ×14 = 42)
(3×

5. Define metabolism. Discuss how the biological energy transformations obey the laws of
thermodynamics.
6. Describe how the glycolytic pathway is regulated.
7. Describe how pyruvate dehydrogenase complex produce acetyl-CoA from pyruvate..
8. Describe the ETS found in mitochondria.

Group (B) Short Questions: (Any six) ×7 = 42)

(6×
9. Why ATP is called as energy currency?
10. How do NADH and NADPH carry biological redox reactions?
11. How do various forms of carbohydrates enter into glycolytic pathway?
12. Describe oxidative reactions of pentose phosphate pathway.
13. Describe the oxidation of malate to oxaloacetate.
14. Describe glyoxylate cycle.
15. Describe malate-aspartate shuttle in mitochondria.
16. Compare the topology of proton movement and ATP synthase orientation in the membranes of
mitochondria, choloroplasts and E.coli.

Group (C) Write short notes on: (Any eight) ×8 = 16)

(2×

13. Define standard free energy change.

14. How does entropy change occur during glucose oxidation?
15. What is the importance of redox potentials of various compounds in the biosphere?
16. What are the fates of pyruvate, the terminal product of glycolytic pathway.
17. What do you mean by fermentation?
18. Give a short account of isozymes.
19. Count the numbers of NADH and FADH2 produced when a glucose molecule is completely
oxidised.
20. Why the TCA cycle is considered as amphibolic pathway?
21. Point out the regulatory sites in the TCA cycle.
22. Draw the structure of ATP synthase complex.
23. Why mitochondria is anlogous to endosymbiotic bacteria?
24. Give a brief account of cytichrome b6 f complex.
***