ARVO 2010 Poster D719

Spatial oxygenation mapping of the optic disc in glaucoma by multispectral imaging

J. Denniss1, I. Schiessl1, V.Nourrit1, C. Fenerty1,2, D.B. Henson3
1Faculty

of Life Sciences, University of Manchester,

Manchester Royal Eye Hospital, 3 School of Biomedicine, University of Manchester, UK

b c a d e

Purpose
To present spatial optic disc oxygenation maps from a group of glaucoma patients and to demonstrate the correspondence between oxygenation maps and structural measures.

Methods
Eleven patients (20 eyes, mean age 71 8 years) with primary open-angle glaucoma, glaucomatous visual field defect, age >40 years, no other ophthalmic disease, and refractive error less than +/-5.00DS equivalent and/or 2.00DC were recruited from the clinics of Manchester Royal Eye Hospital, UK. Patients undertook HRT3 (Heidelberg Engineering GmbH, Germany) and multispectral imaging of the optic disc. Multispectral imaging was carried out with a modified digital fundus camera system incorporating a 150W xenon arc light source and a fasttunable liquid crystal filter (Varispec, Cambridge Research Systems, UK). Images were taken with 8 different wavelengths of light selected according to the reflection properties of retinal blood components (range 496-700nm, total acquisition time <1.68s, figure 3). Pixel-by-pixel oxygenation maps were calculated from the aligned multispectral images using a BeerLambert law calculation.

Figure 1: Two typical examples (above & below) of oxygenation maps (left) in which brighter areas represent areas of greater oxygenation, and corresponding HRT topography images (right) where the red areas represent areas of cupping. Good correspondence can be seen between the dark central area of low oxygen in the oxygenation map and the area of cupping in the topography images.

Figure 2: The multispectral imaging system showing (a) modified fundus camera with (b) peltier-cooled CCD array, (c) fluid optic , (d) fast-tunable liquid crystal filter and (e) light source (250W halogen in this picture).

Current/Future Work
Use of multispectral imaging in glaucoma detection Relationships between oxygenation and visual field loss Use of multispectral imaging to predict treatment efficacy
Figure 3b: (left) The oxygenation map produced from the images in figure 3a and (below left) the corresponding HRT topography. Concordance can be seen between areas of lower oxygenation and areas of cupping.

Results
Oxygenation maps were calculated for all 20 imaged eyes. In all eyes areas of low oxygenation were found to correspond to areas of cupping measured by the HRT3. Figure 1 shows typical examples where in the oxygenation maps brighter areas represent higher oxygenation and darker areas represent lower oxygenation. These correspond well with the HRT3 topography images where the red areas represent the areas of cupping. All patients found the imaging process to be comfortable.

Figure 3a (below): Images from one patient at 8 different wavelengths. The resultant oxygenation map is shown in figure 3b (above right). The most important images for our current analysis are highlighted and represent (left-right) 2 isobestic wavelengths, maximal absorption for oxyhaemoglobin, maximal absorption for deoxyhaemoglobin. Total acquisition time for these images was 1.59 seconds.
496nm 550nm 570nm 575nm 580nm 586nm 610nm 700nm

Conclusions
Spatial oxygenation maps calculated from multispectral images show correspondence to optic disc structures and may provide additional information on optic disc perfusion in glaucoma patients.

Acknowledgements/Support
College of Optometrists (UK) PhD Studentship (JD), Manchester Academic Health Sciences Centre (MAHSC) and the NIHR Manchester Biomedical Research Centre. Commercial Relationships: JD none, IS none, VN none, CF none, DBH none

j.denniss@postgrad.manchester.ac.uk