Jump to: navigation, search Canada balsam, also called Canada turpentine or balsam of fir, is a turpentine which is made from the resin of the balsam fir tree (Abies balsamea). The resin, dissolved in essential oils, is a viscous, sticky, colourless (sometimes yellowish) liquid, that turns to a transparent yellowish mass when the essential oils have been allowed to evaporate. Canada balsam is amorphous when dried. It does not crystallize with age, so its optical properties do not deteriorate.[citation needed] However, it has poor thermal and solvent resistance.[1]
[edit] Uses
Due to its high optical quality and the similarity of its refractive index to that of crown glass (n = 1.55), purified and filtered Canada balsam was traditionally used in optics as an invisible-whendry glue for glass, such as lens elements. The lenses glued with Canada balsam (or with other similar glues) are called cemented lenses. Also other optical elements such as two prisms to form a beam splitter can be cemented with Canada balsam. Balsam was phased out as an optical adhesive during World War II, in favour of polyester, epoxy and urethane-based adhesives. In modern optical manufacturing UV-cured epoxies are often used to bond lens elements. Canada balsam was also used for making permanent microscope slides. In biology, for example, it can be used to conserve microscopic samples. The sample is sandwiched between a microscope slide and a coverslip. Canada balsam is used to glue the arrangement together and enclose the sample to conserve it. Xylene balsam is Canada balsam dissolved in xylene. It is used for preparing slide mounts.[2] Some workers prefer terpene resin as it is both less acidic and cheaper than balsam. Synthetic resins have largely replaced organic balsams for such applications. Some other uses (traditional and current) include:
in geology, it is used as a common thin section cement and glue, and is used for refractive index studies and tests, such as the Becke line test. to fix scratches in glass (car glass for instance) as invisibly as possible. in oil painting, to achieve glow and facilitate fusion. in Buckley's cough syrup.
2. http://www.microbehunter.com/2010/01/23/an-overview-of-mounting-media-formicroscopy/ This post gives an overview of different water-based and non-water-based mounting media and their advantages and disadvantages. Mounting media are needed for making permanent slides. The mounting medium holds the specimens in place between the cover slip and the slide. The choice of the right mounting medium is a separate topic all on its own. There are countless commercial and home-made mounting media available. Which ones should one use? In many cases the microscopist has no choice: some specimens simply require the use of a specific mounting medium, otherwise the structure that one wants to observe is not properly visible. Alternatively, not all specimen types are chemically compatible with the solvents of the medium. Generally, mounting media for permanent slides can be categorized into water-based and organic solvent based mounting media. While many water-based mounting media for permanent slides solidify and hold the specimen firmly in place, some others remain in a liquid state. In this latter case, it is necessary to prevent the liquid from flowing out by sealing the four sides of the cover slip. Nail polish can be used for this. In the following paragraphs, Id like to give you an overview of the different types of mounting media.
Summary: The advantage of Canada balsam is the long storage ability of the slides. Other, modern, mounting media may have a similar storage ability, but with Canada balsam there is historic experience. A disadvantage is the need for toxic solvents when preparing the specimen. Apparently, it is also not very cheap to obtain. Eukitt and other resin-based media: Eukitt is a very fast drying general-purpose resin-based mounting medium. Eukitt will solidify within about 20 minutes. The specimens must be free of water and placed first in alcohol and then in xylene prior to mounting. The use of xylene is a disadvantage, as it is harmful when inhaled. Eukitt itself can also be diluted by xylene to adjust it viscosity. Besides Eukitt, a range of other resin-based mounting media are commercially available, such as Diatex, Entellan, Malinol, Rhenohistol and Depex. They differ in their refractive index. All of these mounting media require the specimen to be first dehydrated in alcohol and then transferred to xylene. Some of these resins shrink significantly during the drying process. Summary: The advantage of Eukitt is that it is a fast drying mounting medium. The disadvantage is the need for toxic solvents to prepare the specimen. Clear nail polish: Nail polish can be used to seal the sides of the coverslip when using aqueous mounting media. It can also be used directly as a mounting medium. The specimens must first be dehydrated in alcohol and can then be directly mounted (without xylene) in nail polish. Summary: The advantage of nail polish is, that it is readily available and that it avoids the use of toxic organic solvents to treat the specimens. One disadvantage is, that it seems to shrink a lot when making very thick mounts (such as whole insects).
Glycerol jelly is one of the most difficult mounting mediums to use, but sometimes there is no other satisfactory alternative to an aqueous mounting medium. Water-based mounting media are useful for making permanent mounts of water organisms, algae, protozoa, etc. Glycerol jelly according to Kisser (not Kaiser) is commonly used to preserve pollen samples. Treating some specimens with organic solvent-based mounting media would cause them to shrink or change their shape in other unacceptable ways. Solvent-base media may also dissolve some of the pigments, such as chlorophyll, from the specimen, which does not happen when using aqueous media such as glycerol jelly. Summary: The advantage of Glycerol jelly is that it s water-based and that this avoids the need of alcohol dehydration (which possibly deforms the specimens), and other toxic organic solvents. Some specimens can only be satisfactorily mounted in Glycerol jelly. It also does not shrink. The disadvantages include the need for a potentially toxic antiseptic in the jelly, the difficulty of mounting the specimens and the need to seal the cover slip with nail polish.