BIOLOGY PROJECT

Created By :

Anggarjuna P. Puji Putra - 03

Aristya P. Marzuki - 04 Mirza B. Firnanda - 18

XII SCIENCE 6
SMAN 5 SURABAYA 2011-2012

Nutrient Testing on Food

A. Goal To know the nutrition of the our daily food. B. Tools and materials

- Sugar

- Blue Band

- Avocado

- Soya Bean

- Rice

- Meat

- Bread

- Orange

- Milk

- Peanut

- Tofu

- Cooking Oil

- Lugol

- Fehling A

- Fehling B

- Biuret

- Reaction Tube

- Baker Glass

- Pipette C. How to work

- Filtration Paper

- Drop plate

1. Test the Amilum/starch concentration of foods : - First materials food to be tested must be crushed or dissolved - Then Squirt Food Samples to be tested into the plate drops using a pipette - Squirt Food Samples would be tested with Lugol's - Observe what happens to the food sample 2. Test the Glucose concentration of foods: - First materials food to be tested must be crushed or dissolved - Then Squirt Food Samples (5 drops) to be tested into the plate drops using a pipette - Squirt Food Samples would be tested with Fehling A (3 drops) and Fehling B (3 drops) - Heat the solution on it while swaying laterally right and left - Observe what happens to the food sample 3. Test the Protein concentration of foods: - First materials food to be tested must be crushed or dissolved - Then Squirt Food Samples to be tested into the plate drops using a pipette - Squirt Food Samples would be tested with Biuret Solution - Observe what happens to the food sample 4. Test the Fat concentration of foods: - First materials food to be tested must be crushed or dissolved - Then Squirt Food Samples to be tested into the plate drops using a pipette - Wait until drops samples are drying - Observe what happens to the food sample

D. Result of Experiment 1. Test the Amilum concentration of foods: - There are several food samples which when dropped with Lugol then the color changed to blue-black. This means the food contains amilum

Amilum experiment table result: No 1 2 3 4 5 6 7 8 9 10 11 12 13 Food sample White of egg Milk Meat Tempe Orange Peanut Rice Tofu Avocado Bread Cooking oil Butter Sugar Color Orange Yellow Orange Brown Yellow Light purple Old purple Old purple Yellow-green Old purple Orange Orange Yellow Explanation Doesnt contain amilum Doesnt contain amilum Doesnt contain amilum Doesnt contain amilum Doesnt contain amilum Containingamilum Containingamilum Containingamilum Doesnt contain amilum Containingamilum Doesnt contain amilum Doesnt contain amilum Doesnt contain amilum

2. Test the Glucose concentration of foods: - There are several food samples which when dropped with Fehling A and Fehling B, then heat that, if the color changed to red brick or orange. This means the food contains glucose.

Glucose experiment table result: No 1 2 3 4 5 6 7 8 9 10 11 12 13 Food sample White of egg Milk Meat Tempe Orange Peanut Rice Tofu Avocado Bread Cooking oil Butter Sugar Color Black Orange Purple Purple Oranee Purple Green Purple Brown-yellow Orange Green Green Orange Keterangan Doesnt contain glucose Contain glucose Doesnt contain glucose Doesnt contain glucose Contain glucose Doesnt contain glucose Doesnt contain glucose Doesnt contain glucose Contain glucose Contain glucose Doesnt contain glucose Doesnt contain glucose Contain glucose

3. Test the Protein concentration of foods: - There are several food samples if the drops with the biuret color changed to a
purplish redcolor. This means the food contains protein.

Protein experiment table result : No 1 2 3 4 5 6 7 8 9 10 11 12 13 Food sample White of egg Milk Meat Tempe Orange Peanut Rice Tofu Avocado Bread Cooking oil Butter Sugar Color Purple Purple Purple Mauve Black Purple Blue Purple Biru Brown Blue Brown Blue Explanation Contain Protein Contain Protein Contain Protein Contain Protein Doesnt contain protein Contain Protein Doesnt contain protein Contain Protein Doesnt contain protein Doesnt contain protein Doesnt contain protein Doesnt contain protein Doesnt contain protein

4. Test the Fat concentration of foods: - There are a few samples of our food is applied to filter paper, if there is scar then he contain
fat.

Fat experiment table result: No 1 2 3 4 5 6 7 8 9 10 11 12 13 Food sample White of egg Milk Meat Tempe Orange Peanut Rice Tofu Avocado Bread Cooking oil Butter Sugar Spot/blot There is There is There is No No There is No No There is No There is There is No Explanation Contain fat Contain fat Contain fat Doesnt contain fat Doesnt contain fat Contain fat Doesnt contain fat Doesnt contain fat Contain fat Doesnt contain fat Contain fat Contain fat Doesnt contain fat

E. Conclusion 1. If there are food that dropped lugol change the color become old blue, these contain starch. if the color doesnt change, that food doesnt contain starch. From that experimentresult, the food that contain starch is: rice, bread, tofu, and peanut 2. If there are food dropped with fehling A and fehling B then burn and shake change that color into red brick or orange, these contain glucose or sugar. From the experiment result, we can found the food contain glucose is: Milk, Orange, Avocado, bread, and sugar. 3. When we drop some biuret in food that contain protein, the color will be change into purple lil bit red. From result above, the food contain protein is: White of egg, milk, meat, tempe, peanut, and tofu. 4. When we put some food with our finger then polished on filter paper and dried it up, it may cause 2 things. First, if we see that paper was become transparent that food contain fat if not, these doesnt contain. From the experiment, the food contain fat is: White of egg, milk, meat, peanut, avocado, cooking oil, and butter.

Effect of Body Weight Against Respiration in Animals and Plants

A. Purpose Observe the relation of body weight on the speed of respiration in animals/plants by using a simple respirometer. B. Whats you need:

A cricket

2 gr sprout

Weight Scale

A respirator board

3 gr KOH(s)

5 ml red eosin

Cotton

Vaseline

C. How to works 1. Weigh the cricket that will tested into scale 2. Packs 3gr solid KOH with cotton. Be carefull, if the KOH interact with our hand may cause irtation. Then, put that and the cricket into respiration tube. 3. Pair the tube with respiration pipe by rubbing Vaseline on the connection area. Its purpose to prevent entry of air. 4. After all is installed on respirator boards, close the edge of the pipe respirator use your fingers for about 1 minute. It aims to crickets spend the oxygen(O2) in the respirator tubes and pipes in order to get perfect calculation. 5. After O2 inside tube and pipe probably used up, inject the red eosin into respirator pipe with until 0 scale. Then watch closely what happen! 6. Do the same way with the sprout. D. Result of experiment Minutes to 1 2 3 The distance of eosin movement Cricket 1.07 gr Sprout 2 gr 0.5 0.03 0.7 0.03 0.8 0.04

4 5 Average per minutes 1. Chart of eosin speed movement

0.7 0.7 0.72

0.02 0.04 0.32

Cricket (1.07 gram)

eosin movement Distance(inch) each minutes Distance(inch) 0.34 0.27 0.2 0.05 1 2 0.12 0.07 3 Minutes to0.08 4 0.07 5 0.07 1

Sprout (2 gram)
eosin movement each minutes 0.16 0.1 0.03 2 0.06 0.03 3 Minutes to0.04 4 0.12 0.02 5 0.04

2. From the graphic, we can conclude that cricket respiration is faster than sprout although sprout more heavier. This happened because the body activity that need oxygen of cricket is more complex than sprout. 3. Role of solid KOH is to absorb CO2 result of respiration. 4. Eosin can move because CO2 result of respiration absorb by KOH. So, air volume inside tube is decreasing and suck the eosin. 5. Beside of body weight, respiration speed is also depended on body activity and metabolism. If there are lot of activity, more O2 is required. E. Conslusion From the experiment above, we can conclude that respiration speed each individual is relative. Its depended on body weight, activity, and metabolism. Fat man needs more air than thin man. In other example, when you are running, your breath becomes faster. It means your body need a lot oxygen. That is the opposite when you are sleeping, doesnt require a lot of air.