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Lead Discovery Target Panels:

Depend on EMD Millipores innovative solutions to deliver results that are core to your success.
voLuME 2, 2011 uPDaTE

EMD Millipore is a division of Merck KGaa, Darmstadt, Germany

Products and Services for Target Profiling and Liability Testing

EMD Millipores Lead Discovery products and services are dedicated to helping you advance those compounds that have the highest chance of being clinically effective and safeas quickly as possible. Whether youre looking to outsource or bring the newest technologies in-house, optimize lead compounds or investigate cardiac safety effects, we can help you generate detailed information about your compounds early in the drug discovery cycle.
NEW! UbiquitinProfiler Service
EMD Millipores ubiquitinProfiler Service is the first and only service that identifies potential activators and inhibitors of E3 ligases within therapeutically promising ubiquitination cascades. See Page 16.

We offer many benefits you wont get anywhere else:


Flexible access to all the major targets, enabling balanced, crosstarget profiling. Validated functional assays provide more information, sensitivity, and biological relevance. Our robust, off-the-shelf cell lines and kits eliminate in-house assay development. FlexLab custom services expand your capacity and capabilities, cost-effectively.
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Early safety assessment across a range of targets. Reliable, quick turnaround keeps your research moving forward. Secure access to data and a new, powerful visualization tool (DART) for better decisions and compelling presentations. Convenient, integrated ordering and invoicing, standardized across the world.

Whats Inside

Cell Lines, Membrane Preps and Profiler Services for: GPCRs Ion Channels Kinases & Phosphatases ubiquitin: ubiquitinProfiler Service
NEW!

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NEW!

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DaRT Target visualization Tool


Introducing DART, the first software application for in-depth visualization of GPCR and kinase profiling data. Our Data Analysis and Report Tool (DART) creates an interactive map of target profiling assay results, enabling you to make faster, more informed decisions in your drug development projects.

DART Target Visualization Tool Specialty Services: FlexLab


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Cross-Target Safety & Liability Screening SignalProfiler


NEW!

See Page 17.

Cell Lines, Membrane Preps and Profiler Services


GPCR Targets
GPCR (Human unless noted) M1 M2 M3 M4 M5 A1 A2A A2B A3 a1A a2A a1B a2B a1D b1 b2 b3 OXGR1/GPR99 C3aR C5aR AT1 AT2 APJ GPBA/GPR131/TGR5 BB1 BB2 BB3 B2 AMY1 CGPR1 CaS CB1 CB2 ChemR23 CCR1 CCR10 CCR2B CCR3 CCR4 CCR5, rhesus macaque CCR6 CCR7 CCR8 CCR9 CX3CR1 GPCRProfiler Service Panel (HTS600GPCR) New FORMAT Available. ChemiScreen Calcium-optimized Cell Lines HTS044C HTS115C HTS116C HTS117C HTS075C HTS047C HTS051C HTS052C HTS087C HTS096C HTS158C HTS216C HTS104C HTS073C HTS159C HTS191C HTS016C HTS017C HTS064C HTS068C HTS123C HTS084C HTS160C HTS041C HTS172C HTS137C HTS019C HTS020C HTS071C HTS005C HTS014C HTS007C HTS008C HTS009C HTS010C HTS011C HTS012C HTS013C HTS036C HTS015C ChemiScreen Membrane Preparations HTS044M HTS115M HTS116M HTS075M HTS047M Ready-to-assay Frozen Cells HTS044RTA HTS115RTA HTS116RTA HTS117RTA HTS075RTA HTS048L HTS052M HTS087M HTS096M HTS158M HTS157M HTS216M HTS104M HTS073M HTS159M HTS016M HTS017M HTS064M HTS208M HTS068M HTS123M HTS084M HTS160M HTS041M HTS127M HTS172M HTS019M HTS020M/HTS020M2 HTS071M HTS005M HTS014M HTS007M HTS008M HTS009M HTS010M HTS011M HTS012M HTS013M HTS036M HTS015M ChemiBrite Stable Cell Lines ChemiBrite Frozen Cells GPCR Family Acetylcholine (muscarinic) Acetylcholine (muscarinic) Acetylcholine (muscarinic) Acetylcholine (muscarinic) Acetylcholine (muscarinic) Adenosine Adenosine Adenosine Adenosine Adrenergic Adrenergic Adrenergic Adrenergic Adrenergic Adrenergic Adrenergic Adrenergic a-Ketoglutarate Anaphylotoxin Anaphylotoxin Angiotensin Angiotensin Apelin Bile Acid Bombesin Bombesin Bombesin Bradykinin Calcitonin Calcitonin Calcium sensor Cannabinoid Cannabinoid Chemoattractant Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine

EMD Millipore has developed proprietary technologies to enhance the functional expression of GPCRs while enabling for a single functional readout regardless of G protein-coupling status. This technology is the foundation of our robust and reliable GPCR products and services.

HTS087RTA HTS096RTA HTS158RTA HTS216RTA HTS104RTA HTS073RTA HTS159RTA HTS191RTA HTS016RTA HTS017RTA HTS064RTA HTS068RTA HTS238L HTS123RTA HTS084RTA HTS160RTA HTS041RTA HTS172RTA HTS137RTA HTS019RTA HTS020RTA HTS071RTA HTS005RTA HTS014RTA HTS007RTA HTS008RTA HTS010RTA HTS011RTA HTS012RTA HTS013RTA HTS036RTA HTS015RTA

New Release Service Available. Cat. No. HTS600GPCR

GPCR Targets
GPCR Family Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Chemokine Cholecystokinin Cholecystokinin CRF Receptor CRF Receptor Dopamine Dopamine Dopamine Dopamine Dopamine Endothelin Endothelin Free Fatty Acid Free Fatty Acid Free Fatty Acid GABAB Galanin Galanin Ghrelin Glucagon Glucagon Glucagon Glucagon Glucagon Glutamate (metabotropic) Glutamate (metabotropic) Glutamate (metabotropic) Glycoprotein hormone GnRH Histamine Histamine Histamine KiSS1-derived peptide Leukotriene Leukotriene Leukotriene Lysophospholipid Lysophospholipid Lysophospholipid Lysophospholipid GPCR (Human unless noted) CXCR1 CXCR2 CXCR3 CXCR4 CXCR5 CXCR6 CXCR7 / RDC1 XCR1 / GPR5 CCK1 CCK2 CRF1 CRF2 D1 D2L D3 D4 D5 ETA ETB GPR41 GPR43 GPR120 GABAB1b GAL1 GAL2 Ghrelin / GHSR-1a GIP GLP-1 GLP-2 glucagon secretin receptor mGlu1 mGlu2 mGlu6 TSH GnRH H1 H2 H3 KiSS1 / GPR54 BLT1 CysLT1 CysLT2 LPA1 LPA3 GPR92 / LPA5 S1P1 GPCRProfiler Service Panel (HTS600GPCR) New FORMAT Available. ChemiScreen Calcium-optimized Cell Lines HTS001C HTS002C HTS003C HTS004C HTS055C HTS054C HTS053C HTS184C HTS083C HTS023C HTS024C HTS102C HTS039C/HTS039C2 HTS223C HTS129C HTS070C HTS046C HTS135C HTS063C HTS119C HTS094C HTS186C HTS187C HTS134C HTS163C/HTS163C2 HTS164C HTS112C HTS174C HTS145C HTS146C HTS133C HTS027C HTS050C HTS086C HTS029C HTS032C HTS042C HTS061C HTS088C HTS089C HTS130C HTS209C HTS176C ChemiScreen Membrane Preparations HTS001M HTS002M HTS003M HTS004M HTS055M HTS054M HTS138M HTS184M HTS083M HTS023M HTS024M HTS102M HTS039M HTS103M HTS223M HTS129M HTS070M HTS046M HTS135M HTS063M HTS119M HTS094M HTS186M HTS187M HTS134M HTS163M HTS112M Ready-to-assay Frozen Cells HTS001RTA HTS002RTA HTS003RTA HTS004RTA HTS055RTA HTS054RTA HTS053RTA HTS184RTA HTS083RTA HTS023RTA HTS024RTA HTS102RTA HTS039RTA HTS223RTA HTS129RTA HTS070RTA HTS046RTA HTS135RTA HTS063RTA HTS225L HTS119RTA HTS094RTA HTS186RTA HTS187RTA HTS134RTA HTS164RTA HTS112RTA HTS174RTA HTS146M HTS149M HTS027M HTS050M HTS086M HTS146RTA HTS133RTA HTS027RTA HTS134LT HTS163LT HTS112LT ChemiBrite Stable Cell Lines ChemiBrite Frozen Cells HTS001LT

HTS004LT

HTS102LT HTS039LT

HTS042M HTS061M HTS089M

HTS176M

HTS032RTA HTS042RTA HTS061RTA HTS088RTA HTS089RTA HTS130RTA HTS209RTA HTS176RTA

New Release Service Available. Cat. No. HTS600GPCR

Cell Lines, Membrane Preps and Profiler Services


GPCR Targets
GPCR (Human unless noted) S1P2 S1P3 S1P4 S1P5 MRGX1 / MRGPRX1 MrgD MRGX2 / MGRPLX2 MCHR1 / GPR24 MCHR2 MC1 MC2 MC3 MC4 MC5 MT1 MT2 motilin receptor, MTLR NMU1 NMU2 NPBW1 / GPR7 NPBW2 / GPR8 Y2 Y4 NTR1 FPR1 FPRL1 GPR109A d k NOP/ORL1 OX1 OX2 GPR39 GPR119 OT P2Y1 P2Y2 P2Y4 P2Y11 P2Y12 GPR103/QRFP PAF GPCRProfiler Service Panel (HTS600GPCR) ChemiScreen Calcium-optimized Cell Lines HTS078C HTS097C HTS192C HTS193C HTS122C HTS206C HTS058C HTS025C HTS026C HTS021C HTS105C HTS155C HTS022M HTS105M HTS155M HTS065M HTS106M HTS121M HTS062M HTS098M HTS180M HTS181M HTS066M HTS204M HTS034M HTS018M HTS056M HTS201M HTS100M HTS095M HTS101M HTS040M ChemiScreen Membrane Preparations HTS078M HTS097M HTS193M HTS122M HTS206M HTS025M HTS156M HTS021RTA Ready-to-assay Frozen Cells HTS078RTA HTS097RTA HTS192RTA HTS193RTA HTS122RTA HTS206RTA HTS058RTA HTS025RTA HTS026RTA ChemiBrite Stable Cell Lines ChemiBrite Frozen Cells HTS078LT

GPCR Family Lysophospholipid Lysophospholipid Lysophospholipid Lysophospholipid Mas-related gene Mas-related gene Mas-related gene Melanin-concentrating hormone Melanin-concentrating hormone Melanocortin Melanocortin Melanocortin Melanocortin Melanocortin Melatonin Melatonin Motilin Neuromedin U Neuromedin U Neuropeptide B/W Neuropeptide B/W Neuropeptide Y Neuropeptide Y Neurotensin N-formylpeptide N-formylpeptide Nicotinic Acid Opioid Opioid Opioid Opioid Orexin Orexin Orphan Orphan Oxytocin P2Y / Purinergic (metabotropic) P2Y / Purinergic (metabotropic) P2Y / Purinergic (metabotropic) P2Y / Purinergic (metabotropic) P2Y / Purinergic (metabotropic) Peptide P518 Platelet Activating Factor

HTS155RTA

New FORMAT Available.

HTS121C HTS062C HTS098C HTS180C HTS066C HTS204C HTS034C HTS018C HTS056C HTS201C HTS100C HTS095C HTS101C HTS040C HTS175C HTS045C HTS217C HTS090C HTS049C HTS210C HTS211C HTS213C HTS195C HTS189C HTS076C

HTS121RTA HTS062RTA HTS098RTA HTS180RTA HTS066RTA HTS204RTA HTS034RTA HTS018RTA HTS056RTA HTS201RTA HTS100RTA HTS101RTA HTS040RTA HTS175RTA HTS045RTA HTS217RTA HTS198L HTS090RTA HTS049RTA HTS210RTA HTS211RTA

HTS090M

HTS049L HTS210L HTS211L HTS213L HTS195L

HTS189M

HTS189RTA HTS076RTA

New Release Service Available. Cat. No. HTS600GPCR

GPCR Targets
GPCR (Human unless noted) PK1 PK2 PRP CRTH2 DP EP1 EP2 EP3 EP4 FP IP1 TP PAR2 Thrombin and Trypsin activated PARs PTH1 PTH2 5-HT1A 5-HT1B 5-HT2A 5-HT2B 5-HT2C 5-HT4 5-HT5A 5-HT6 5-HT7 sst1 sst2 sst3 sst4 sst5 GPR68/OGR1 SUCNR1/GPR91 NK1 NK2 NK3 TRH UT / GPR14 V1A V1A, rat V1B V2 PAC1 long isoform VPAC1 VPAC2 GPCRProfiler Service Panel (HTS600GPCR) HTS030C HTS173C HTS107C HTS082C HTS109C HTS132C HTS110C HTS111C HTS030M HTS030RTA HTS173RTA ChemiScreen Calcium-optimized Cell Lines HTS074C HTS182C HTS057C HTS091C HTS099C HTS185C HTS092C HTS142C HTS093C HTS131C HTS081C ChemiScreen Membrane Preparations HTS074M HTS182M HTS057M HTS031M HTS091M HTS099M HTS185M HTS092M HTS142M Ready-to-assay Frozen Cells HTS074RTA HTS182RTA HTS057RTA HTS091RTA HTS099RTA HTS185RTA HTS092RTA HTS142RTA HTS093RTA HTS131RTA HTS081RTA HTS091L HTS099LT HTS185L HTS092L HTS142L HTS131L HTS081L HTS092LT ChemiBrite Stable Cell Lines ChemiBrite Frozen Cells

GPCR Family Prokineticin Prokineticin Prolactin-releasing peptide Prostanoid Prostanoid Prostanoid Prostanoid Prostanoid Prostanoid Prostanoid Prostanoid Prostanoid Protease-activated Protease-activated PTH receptor PTH receptor Serotonin Serotonin Serotonin Serotonin Serotonin Serotonin Serotonin Serotonin Serotonin Somatostatin Somatostatin Somatostatin Somatostatin Somatostatin SPC/LPC /proton-sensor Succinate Tackykinin /neurokinin Tackykinin /neurokinin Tackykinin /neurokinin TRH Urotensin II Vasopressin Vasopressin Vasopressin Vasopressin VIP/PACAP VIP/PACAP VIP/PACAP

VOTED
Most trusted and most used outsourced GPCR fee-for-service provider.
GPCR Screening & Profiling Trends HTStec Limited, 2010

HTS081M HTS037M

HTS108M HTS082M HTS132M HTS110M HTS141M HTS111M HTS215M HTS170M HTS028M HTS171M HTS125M HTS139M

HTS082RTA HTS109RTA HTS132RTA HTS110RTA HTS111RTA

New FORMAT Available.

HTS028C HTS171C HTS125C HTS139C HTS153C HTS241C HTS080C HTS144C HTS190C HTS126C HTS033C HTS059C HTS177C HTS136C HTS060C HTS114C HTS043C HTS079C

HTS080M HTS144M

HTS028RTA HTS171RTA HTS125RTA HTS139RTA HTS153RTA HTS241RTA HTS080RTA HTS144RTA HTS190RTA HTS126RTA HTS059RTA HTS036RTA HTS060RTA HTS114RTA HTS043RTA HTS079RTA

HTS033M HTS059M HTS136M HTS060M HTS114M HTS043M HTS079M

New Release Service Available. Cat. No. HTS600GPCR

Cell Lines, Membrane Preps and Profiler Services


GPCR Targets
GPCR Family CHEM1, parental CHEM2, parental CHEM3, parental CHEM4, parental CHEM5, parental CHEM9, parental CHEM10, parental HEK293, parental ChemiScreen Parental Cell Lines HTSCHEM-1 HTSCHEM-2 HTSCHEM-3 HTSCHEM-4 HTSCHEM-5 HTSCHEM-9 HTSCHEM-10 ChemiScreen Membrane Preparations HTS000MC1 HTS000MC2 Ready-to-assay Frozen Cells HTSCHEM-1RTA ChemiBrite Parental Cell Lines ChemiBrite Frozen Cells Media

HTSHEK-1L HTSHEK-2L HTSHEK-3L HTSHEK-4L HTSHEK-6L HTSHEKT-1LT, HTSHEKT-2LT, HTSHEKT-3LT, HTSHEKT-4LT HTS000MC HTSCHO-1L HTSU20S-1L HTSCHEM-3S New FORMAT Available.

HEK293T, parental CHO, parental U2OS, parental CHEM3 100x Growth Supplement New Release Service Available. Cat. No. HTS600GPCR

Order online
Products: www.millipore.com/leaddiscovery Services: www.millipore.com/portal Download the appropriate service request form at EMD Millipores Drug Discovery and Development Service Portal.
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Cell Lines, Membrane Preps and Profiler Services


Ion Channel Targets: Sodium Channels
Description hNav1.1 hNav1.2 hNav1.3 hNav1.4 hNav1.5 hNav1.6 hNav1.7 hNav1.8/b1 rNav1.8 Manual Patch Clamp Service CYL6009MPC CYL6023MPC CYL6003MPC CYL6024MPC CYL6004MPC CYL6010MPC CYL6011MPC CYL6025MPC CYL6050MPC PatchXpress Service IonWorks Service CYL6009QU CYL6023QU CYL6003QU CYL6024QU CYL6004QU CYL6010QU CYL6011QU FLIPR Service PrecisIoN Cell Lines CLY3009 CLY3032 CLY3003 CLY3024 CLY3004 CYL3010 CLY3011 CYL3025 CYL3050 Ready-to-assay Frozen Cells CYL3009RTA CYL3023RTA CYL3003RTA CYL3024RTA CYL3004RTA CYL3011RTA

EMD Millipore offers a fully validated panel of functional profiling assays which employ a variety of PrecisIoN ion channel cell lines, the IonWorks HT/Quattro instrument platforms, the PatchXpress instrument, and conventional manual electrophysiology platforms. The cell lines were carefully selected for optimal performance on the IonWorks instrument thus ensuring a high level of accuracy and precision in the assays. our PrecisIoN Ion Channel Cell Lines can be provided as frozen Ready-to-assay cells to existing license holders, for bulk orders or as a supply agreement.

CYL6004PX CYL6011PX CYL6025PX CYL6050PX

Ion Channel Targets: Potassium Channels


Description hERG-CHO hERG-HEK hKCNQ1/hminK hKir2.1 hKv1.1 hKv1.2 hKv1.3 hKv1.4 hKv1.5 hKv1.6 hKv1.7 hKv1.8 hKv2.1 hKv2.1/hKv9.2 hKv3.1 hKv3.2 hKv3.3 hKv4.2/hKChIP2 hKv4.3/hKChIP1 hKv4.3/hKChIP2 hKv7.2/hKv7.3 hKv7.3/hKv7.5 hKv7.4 hKv7.4/hKv7.5 hKv12.2 Manual Patch Clamp Service CYL5038MPC CYL5039MPC CYL5007MPC CYL5032MPC CYL6014MPC CYL6015MPC CYL6016MPC CYL6017MPC CYL5018MPC CYL6019MPC CYL6020MPC CYL6021MPC CYL6022MPC CYL6067MPC CYL6043MPC CYL6044MPC CYL6045MPC CYL6026MPC CYL6027MPC CYL5069MPC CYL6059MPC CYL6060MPC CYL6092MPC CYL6096MPC PatchXpress Service CYL5039PX CYL5007QU CYL5032QU CYL6014QU CYL6015QU CYL6016QU CYL6017HT CYL5018QU CYL6019HT CYL6020HT CYL6021HT CYL6022HT CYL6043QU CYL6044QU CYL6045QU CYL6027QU CYL5069QU CYL6059QU CYL6060QU CYL6092QU IonWorks Service CYL5038QU FLIPR Service PrecisIoN Cell Lines CYL3038 CYL3039 CYL3007 CYL3032 CYL3014 CYL3015 CYL3016 CYL3017 CYL3018 CYL3019 CYL3020 CYL3021 CYL3022 CYL3067 CYL3043 CYL3044 CYL3045 CYL3026 CYL3027 CYL3069 CYL3059 CYL3060 CYL3092 CYL3096 CYL3089 Ready-to-assay Frozen Cells CYL3038RTA CYL3007RTA CYL3032RTA CYL3014RTA CYL3016RTA CY3018RTA

CYL3022RTA

CYL3069RTA

Cell Lines, Membrane Preps and Profiler Services


Ion Channel Targets: Calcium Channels
Description hL-type Calcium hN-type Calcium hT-type Calcium Manual Patch Clamp Service CYL5051MPC CYL6054MPC CYL6075MPC PatchXpress Service Inquire IonWorks Service CYL5051QU CYL6054QU CYL6075QU FLIPR Service PrecisIoN Cell Lines Inquire CYL3054 CYL3075 Ready-to-assay Frozen Cells

Ion Channel Targets: HCN Channels


Description hHCN1 hHCN2 hHCN3 hHCN4 Manual Patch Clamp Service CYL6040MPC CYL6041MPC CYL6042MPC CYL5012MPC PatchXpress Service IonWorks Service CYL6040HT CYL6041HT CYL5012HT FLIPR Service PrecisIoN Cell Lines CYL3040 CYL3041 CYL3042 CYL3012 Ready-to-assay Frozen Cells

CYL3012RTA

Ion Channel Targets: Ligand Gated Channels


Description hASIC3 hCFTR hGABAA a1/b3/g2 hGABAA a2/b3/g2-HEK hGABAA a3/b3/g2 hGABAA a4/b3/g2-HEK hGABAA a5/b3/g2 hGABAA a6/b3/g2-HEK hGluR6 hGlyRA1 hnAChR a1/b1/d/e hnAChR a3/b4 Manual Patch Clamp Service CYL8055MPC CYL8088MPC CYL8053MPC CYL8072MPC CYL8068MPC CYL8085MPC CYL8073MPC CYL8086MPC CYL8049MPC CYL8056MPC CYL8052MPC CYL8057MPC CLY8049PX CYL8068PX CYL8068QU CLY8053PX CYL8053QU PatchXpress Service IonWorks Service FLIPR Service PrecisIoN Cell Lines CYL3055 CYL3088 CYL3053 CYL3072 CYL3068 CYL3085 CYL3073 CYL3086 CYL3049 CYL3056 CYL3052 CYL3057 Ready-to-assay Frozen Cells

Ion Channel Targets: TRP Channels


Description hTRPA1 hTRPV1 hTRPV3 Manual Patch Clamp Service CYL8066MPC CYL8063MPC CYL8065MPC PatchXpress Service CYL8063PX IonWorks Service FLIPR Service CYL8066FL CYL8063FL CYL8065FL PrecisIoN Cell Lines CYL3066 CYL3063 CYL3065 Ready-to-assay Frozen Cells

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Order online
Products: www.millipore.com/leaddiscovery Services: www.millipore.com/portal
CardiacProfiler Service
The IonChannelProfiler Service is offered as a monthly profiling service that allows for the comprehensive functional assessment of drug candidates against hERG, hHCN4, hKCNQ1/hminK, hKir2.1, hKv1.5, hKv4.3/ hKChIP2, hNav1.5, and Human L-type Calcium ion channels. Collectively, these cardiac ion channels play a critical role in the normal functioning of the human heart and were chosen to provide a more thorough assessment of the true cardiac liability of drug candidates.
Description Panel profiling service against hERG, hHCN4, hKCNQ1/hminK, hKir2.1, hKv1.5, hKv4.3/hKChIP2, hNav1.5, and Human L-type Calcium IonWorks Service CLY9000

Download the appropriate service request form at EMD Millipores Drug Discovery and Development Service Portal.

PrecisIoN Recombinant hERG Potassium Ion Channel Membrane Preparation


To compliment our functional assays, EMD Millipore provides membranes for hERG radioligand binding assays. These have been validated using several different radioligands and provide comparable data found in published literature. While not providing a functional measure of hERG activity, the very high throughput of radioligand binding assays have made this a very useful approach for monitoring potential hERG liability at the earliest phase of drug discovery. Here, the primary aims are to raise flags which could trigger follow-up studies, such as electrophysiological screening.
Description hERG Species human Background Cell Type HEK Cell Line Catalog No. CYL4039

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Enzymes and Profiler Services


Kinase Targets
Enzyme Abl, active Abl, active, mouse Abl (H396P), active Kinase Catalogue Profiler No. Service 14-529 14-459 14-750 14-757 14-751 14-522 14-759 14-756 14-341 14-453 14-276 14-279 14-241 14-339 14-502 14-416 14-555 14-614 14-840 14-902 14-521 14-460 14-661 14-606 14-511 14-835 14-911 14-512 14-517 14-316 14-499 assay Kit Substrate 12-493, 12-525, 12-539 12-493, 12-525, 12-539 12-493, 12-525, 12-539 12-493, 12-525, 12-539 12-493, 12-525, 12-539 12-493, 12-525, 12-539 12-493, 12-525, 12-539 Kinase Catalogue Profiler Enzyme No. Service B-Raf (V599E), active 14-557 B-Raf (1-415), active 14-530 Brk, active 14-613 BrSK1, active 14-675 BrSK2, active 14-655 BTK, active 14-552 BTK (E41K), active 14-773 BTK (R28H), active 14-765 CaM Kinase I, active 14-663 CaM Kinase IIb, active CaM Kinase IIg, active CaM Kinase IId, active CaM Kinase IV, active CaM Kinase Id, active Casein Kinase 1, active, yeast Casein Kinase 1g1, active Casein Kinase 1g2, active Casein Kinase 1g3, active Casein Kinase 1d (aa 1-294), active Casein Kinase 2, active Casein Kinase 2a, active Casein Kinase 2a2, active cdk1/cyclin B, active cdk2/cyclin A, active cdk2/cyclin E, active cdk3/cyclin E, active cdk5/p25, active cdk5/p35, active cdk6/cyclin D3, active cdk7/cyclin H/MAT1 (CAK complex), active cdk9/cyclin T1, active CHK1, active CHK2, active 12-516 CHK2 (I157T) CHK2 (R145W) c-Kit (D816H), active 14-718 14-719 14-723 14-547 14-731 14-112 14-711 14-712 14-713 14-520 14-197 14-445 14-689 14-450 14-448 14-475 14-487 14-516 14-477 14-519 14-476 14-685 14-346 14-347 14-741 14-740 14-726 assay Kit Substrate Enzyme c-Kit (D816V), active c-Kit (V560G), active c-Kit (V654A), active c-Kit, active CLK2, active CLK3, active Cot1, active CSK, active C-TAK1 (cdc25C associated protein kinase) DAPK1, active DAPK2, active DCAMKL2, active DDR2, active DMPK, active DRAK1, active DYRK1A, active DYRK2, active eEF-2K, active EGFR, active EGFR (L858R), active EGFR (L861Q), active EGFR (T790M), active EGFR (T790M, L858R), active EphA1, active EphA2, active EphA3, active EphA4, active EphA5, active EphA7, active EphA8, active EphB1, active EphB2, active EphB3, active EphB4, active erbB-4/HER-4, active FAK, active Fer, active FES/FPS, active FGFR1, active FGFR1 (V561M), active FGFR2, active FGFR2 (N549H), active FGFR3, active FGFR4, active Fgr, active Flt-1, active Flt-3 (D835Y), active Flt-3, active Flt-4, active Kinase Catalogue Profiler No. Service 14-611 14-730 14-733 14-559 14-774 14-724 14-703 14-458 14-375 14-692 14-657 14-716 14-579 14-649 14-668 14-435 14-669 14-654 14-531 14-626 14-627 14-725 14-721 14-653 14-560 14-644 14-574 14-639 14-672 14-673 14-674 14-553 14-561 14-554 14-569 14-720 14-605 14-473 14-582 14-734 14-617 14-742 14-464 14-583 14-568 14-562 14-610 14-500 14-681 assay Kit Substrate

EMD Millipore has a broad and continuously expanding portfolio of recombinant proteins. This portfolio includes more than 350 active, inactive and unactivated kinases, phosphatases and the largest available selection of diseaserelevant mutants. all of our enzymes are available in quantities sufficient for high throughput screening.

Abl (M351T), active Abl (Q252H), active Abl (T315I), active Abl (Y253F), active ACK1, active Akt1/PKBa (PH), active Akt1/PKBa (PH, S473D), active Akt1/PKBa, active Akt1/PKBa, unactive Akt1/PKBa-GST, unactivated agarose conj. Akt2/PKBb (PH, S474D), active Akt3/PKBg (S472D), active Akt3/PKBg, unactive ALK, active ALK4, active AMPK (a1, b1, g1), active AMPK (a2, b1, g1), active Arg, active, human Arg, active, mouse ARK5, active ASK1, active Aurora A, active Aurora B, active Aurora C, active Axl, active Blk, active, human Blk, active, mouse Bmx, active

12-433, 12-451 12-433, 12-451 12-433, 12-451 12-433, 12-451

12-423, 12-529 12-423, 12-529 12-423, 12-529 12-423, 12-529 12-423, 12-529 12-330 12-330 12-330

12-550

12-355 12-355

12-526

12-373, 12-414 12-373, 12-414 12-373, 12-414 12-373, 12-414

12

Service Available. No catalogue number required.

Kinase Targets
Enzyme Fms, active Fms (Y969C), active Fyn, active Fyn, partially purified GCK, active GRK5, active GRK6, active GRK7, active GSK3a, active GSK3b, active GSK3b, active, rat Haspin, active Hck, activated Hck, active HIPK1, active HIPK2, active HIPK3, active IGF-1R, activated IGF-IR, active IKKa, active IKKb, active Insulin Receptor, activated Insulin Receptor, active IRAK1, active IRAK4, active IRR, active Itk, active JAK2, active JAK2, immune complex agarose JAK3, active JNK1a1/SAPK1c, active JNK1a1/SAPK1c, unactive JNK2a2/SAPK1a, active JNK2a2/SAPK1a, unactive JNK3/SAPK1b (K55R), inactive JNK3/SAPK1b, active JNK3/SAPK1b, unactive KDR, active Lck, activated Lck, active Lck, unactive Kinase Catalogue Profiler No. Service 14-551 14-820 14-441 14-107 14-743 14-714 14-715 14-752 14-492 14-306 14-538 14-744 14-843 14-577 14-679 14-623 14-680 14-802 14-465 14-461 14-485 14-803 14-466 14-684 14-599 14-645 14-660 14-640 14-134 14-629 14-327 14-328 14-329 14-330 14-239 14-501 14-523 14-630 14-842 14-442 14-708 12-528 12-528 12-528 assay Kit Substrate Enzyme LIM Kinase 1, active LIM Kinase 1, active, mouse LIM Kinase 1, unactive LKB1/STRADa/ MO25a, active LOK, active Lyn, active Lyn, active, mouse Lyn (p56), partially purified MAP Kinase 1/Erk1, active MAP Kinase 1/Erk1, unactive MAP Kinase 1/Erk1 agarose, unactive MAP Kinase 2/Erk2, active MAP Kinase 2/Erk2, active, mouse MAP Kinase 2/Erk2, unactive MAP Kinase 2/Erk2, unactive, mouse MAPKAP Kinase 2, active MAPKAP Kinase 2, unactive MAP Kinase 2/Erk 2 (K52R), Inactive MAPKAP Kinase 3, active MAPKAP Kinase 3, unactive MARK1, active MEK1, active MEK1, unactive MEK1 (K97R), inactive MEK2, active MEK2, unactive MEK2, unactive MEK2 (K101M), inactive MELK, active Mer, active Met, active Met (D1246H), active Met (D1246N), active Met (M1268T), active Met (Y1248C), active Met (Y1248D), active Kinase Catalogue Profiler No. Service 14-656 14-457 14-659 14-596 14-686 14-510 14-315 14-105 14-439 14-515 14-121 14-550 14-173 14-536 14-198 14-337 14-349 14-696 14-585 14-586 14-651 14-429 14-420 14-737 14-528 14-532 14-541 14-709 14-909 14-728 14-526 14-915 14-818 14-817 14-805 14-816 assay Kit Substrate Enzyme Met (Y1248H), active MINK, active MKK4/SKK1, active, mouse MKK4/SKK1, unactive, mouse MKK6/SKK3 (S599D, T603D), active MKK6/SKK3, active MKK6/SKK3, unactive MKK7b1, active MKK7b1, unactive MLCK, active MLK1, active Mnk2, active MRCKa, active MRCKb, active MSK1, active MSK2, active MSK2, unactive MSSK1, active MST1, active MST2, active MST3, active mTOR mTOR (1362-end) / FKBP12 mTOR (1362-end), active mTOR / FKBP12 MuSK, active NEK2, active NEK3, active NEK6, active NEK7, active NEK11, active NLK, active p38a/SAPK2a, active p38a/SAPK2a, unactive p38a/SAPK2a (6Histag), active p38a/SAPK2a (6Histag), unactive p38a/SAPK2a (T106M), active p38b2/SAPK2b2, active p38g/SAPK3, active p38d/SAPK4, active Kinase Catalogue Profiler No. Service 14-804 14-615 14-377 14-378 14-537 14-303 14-304 14-543 14-542 14-638 14-690 14-664 14-691 14-643 14-548 14-616 14-625 14-665 14-624 14-524 14-695 14-784 14-770 assay Kit Substrate

12-533 12-533 12-533

12-527 12-527 12-459 12-460

12-331, 12-385

12-645 12-645 12-645 12-645

12-547

14-634 14-545 14-694 14-578 14-565 14-700 14-676 14-251 14-252 14-587 14-588 14-687 14-253 14-246 14-249

12-544

Service Available. No catalogue number required.

13

Enzymes and Profiler Services


Kinase Targets
Kinase Catalogue Profiler Enzyme No. Service p70 S6 Kinase (T412E), 14-486 active PAK2, active 14-481 PAK3, active 14-683 PAK4, active 14-584 PAK5, active 14-699 PAK6, active 14-633 PAR-1Ba, active 14-544 PASK, active 14-701 14-467 PDGFRa, active 14-735 PDGFRa (550-end, V561D), active human 14-729 PDGFRa (D842V), active 14-463 PDGFRb, active PDK1, active 14-452 PEK, active 14-916 14-698 PhKg2, active 14-782 PI3 Kinase (p110a (E542K)/p85a), active, human 14-791 PI3 Kinase (p110a (E542K)/p85a), mouse 14-783 PI3 Kinase (p110a (E545K)/p85a), active, human 14-781 PI3 Kinase (p110a (E545K)/p85a), active, mouse 14-787 PI3 Kinase (p110a (H1047R)/p85a), active, mouse 14-792 PI3 Kinase (p110a (H1047R)/p85a), human 14-790 PI3 Kinase (p110a/ p65a), active, human 14-786 PI3 Kinase (p110a/ p65a), active, mouse 14-602 PI3 Kinase (p110a/ p85a), active, human 14-785 PI3 Kinase (p110a/ p85a), active, mouse 14-794 PI3 Kinase (p110b/ p85a), active, mouse 14-603 PI3 Kinase (p110b/ p85a), active, human 14-788 PI3 Kinase (p110b/ p85a), active, mouse 14-789 PI3 Kinase (p110d/ p85a), active, mouse assay Kit Substrate 12-420 Enzyme PI3 Kinase (p110d/ p85a), active, human 14-558 PI3 Kinase (p120g), human PI3 Kinase C2a, active 14-906 PI3 Kinase C2b, active 14-907 PI3 Kinase C2g, active PI4KIIIa, active Pim-1, active Pim-2, active Pim-3, active PIP4K2a, active PIP5K1a, active PIP5K1g, active PKA, catalytic subunit, recombinant PKC, active 14-910 14-908 14-573 14-607 14-738 14-901 14-844 14-845 14-440 14-115 Kinase Catalogue Profiler No. Service 14-604 assay Kit Substrate 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 Enzyme Ret (V804M), active Ret, active RIPK2, active ROKa/ROCK-II, active ROKa/ROCK-II, active, rat ROKb/ROCK-I, active Ron, active Ros, active Rse, active Rsk1/MAPKAP Kinase 1a, active Rsk1/MAPKAP Kinase 1a, active, rat Rsk2/MAPKAP Kinase 1b, active Rsk3, active Rsk4, active SGK1 (1-59, S422D), active SGK1 (1-59, S422D), unactive SGK2, active SGK2, unactive SGK3, active SGK3, unactive SIK, active Snk, active Src, active Src (1-530), active Src (p60c-src) Src (T341M), active SRPK1, active SRPK2, active STK33, active Syk, active TAK1-TAB1 fusion, active TAO1, active TAO2, active TAO3, active TBK1, active Tec, activated TGFBR1, active Tie2, active Tie2 (R849W), active Tie2 (Y1108F) Tie2 (Y897S) TLK2, active TrkA, active TrkB, active TSSK1, active Kinase Catalogue Profiler No. Service 14-760 14-570 14-612 14-451 14-338 14-601 14-581 14-527 14-535 14-509 14-479 14-480 14-462 14-702 14-331 14-332 14-635 14-636 14-647 14-648 14-652 14-637 14-326 14-746 14-117 14-748 14-564 14-666 14-671 14-314 14-600 14-749 14-736 14-745 14-628 14-801 14-912 14-540 14-763 14-766 14-764 14-739 14-571 14-507 14-670 12-243 assay Kit Substrate

12-124

12-542

12-401

17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495 17-494, 17-495

12-151, 12-152, 12-394 12-121, 12-123, 12-411, 12-536

PKCa, active PKCbI, active PKCbII, active PKCg, active PKCd, active PKC, active PKC, active PKC, active PKC, active PKC, active PKC, active PKD2, active PKG1a, active PKG1b, active PKR agarose, active Plk1, active Plk3, active PRAK, active PRAK, unactive PRK2, active PRKX, active PTK5, active Pyk2, active Raf-1 (truncated), active Ret (V804L), active

14-484 14-503 14-496 14-483 14-504 14-518 14-525 14-497 14-444 14-505 14-508 14-506 14-688 14-650 14-423 14-777 14-572 14-334 14-335 14-549 14-677 14-693 14-567 14-352 14-758

12-543

12-532

12-363 12-363

14

New Release Service Available. No catalogue number required.

Phosphatase Targets
Enzyme TSSK2, active Txk, active ULK2, active ULK3, active VRK2, active Wee1, active WNK2, active WNK3, active Yes, active ZAP-70, active ZIPK, active Kinase Catalogue Profiler No. Service 14-632 14-761 14-772 14-755 14-732 14-408 14-678 14-658 14-478 14-404 14-608 assay Kit Substrate Enzyme Calcineurin/PP2B, active Calcineurin/PP2B, active CD45 cdc25A, active cdc25B, active DUSP22 HePTP Lambda Protein Phosphatase Lambda Protein Phosphatase, GST LMPTP-A LMPTP-B MKP-1/CL100, active MKP5, active PP1, purified PP1a PP2A, purified PP5, active PTEN, active PTP-1B PTP-1B PTP-1B, active, agarose conj. PTPb PTPMEG-1 PTPMEG-2 PTPN22 RPTP, active SHP-1/SHPTP-1 SHP-1 SHP-2 TCPTP TMDP VHR YopH, active Service Available. No catalogue number required. Catalogue No. 14-390 14-446 14-618 14-369 14-370 14-641 14-593 14-405 14-598 14-619 14-620 14-391 14-779 14-110 14-595 14-111 14-778 14-488 14-358 14-621 14-109 14-350 14-642 14-592 14-768 14-780 14-113 14-591 14-622 14-646 14-767 14-594 14-590 PhosphataseProfiler Service

VOTED
Most preferred and most used fee-for-service kinase profiling provider.
Kinase Screening & Profiling Trends, HTStec Limited, 2010.

12-545

Order online
Products: www.millipore.com/leaddiscovery Services: www.millipore.com/portal Download the appropriate service request form at EMD Millipores Drug Discovery and Development Service Portal.
15

ubiquitin
ubiquitinProfiler: at a Glance
Profile your leads against our expanding panel of E3 ligases, using a physiologically relevant E1-E2-E3 ligase cascade Obtain single point inhibition data or 9-point IC50 curves Measure substrate ubiquitination with validated activity assays Get data reports in 4 weeks or less

NEW!
ubiquitinProfiler Service accelerate ubiquitin-related drug discovery with our innovative, functional assay service that reduces the complexity of the ubiquitination pathway. EMD Millipore simplifies your route to developing effective, safe ubiquitination-targeted therapeutics. ubiquitinProfiler is the first and only service that identifies potential activators and inhibitors of E3 ligases within therapeutically promising ubiquitination cascades.

Cascades
E1 ligase Ube1 Ube1 Ube1 Ube1 Ube1 Ube1 ube1

NoW avaILaBLE
E2 ligase UbcH3 UbcH3 UbcH3 UbcH4 UbcH4 UbcH3 ubcH5a E3 ligase SCFSkp2/Cks1 SCFbTrCP1 SCF SCF
bTrCP1 bTrCP1

Substrate p27 b-catenin IkBa b-catenin IkBa Cyclin E Cyclin E

SCFbTrCP1 SCFFbw7 SCF


Fbw7

Cascades
E1 ligase UBE1 UBE1 UBE1 UBE1 UBE1 UBE1 UBE1 UBE1 UBE1

IN DEvELoPMENT
E2 ligase UbcH4 UbcH5c UbcH4 UbcH5c UbcH4 UbcH4 UbcH4 UbcH5a UbcH5c E3 ligase MDM2/CK1d MDM2/CK1d MDM2 MDM2 c-Cbl c-Cbl VHL VHL VHL Substrate p53 p53 (auto-ubiquitination) (auto-ubiquitination) Src Kit HIF-1a HIF-1a HIF-1a

GET STARTED
visit EMD Millipores Drug Discovery and Development Portal to download a request form - www. millipore.com/portal, or email ubiquitinprofiler@ merckgroup.com.

16

Specialty Services
FlexLabSM Service
Obtaining the reagents you need to drive your discovery program is not limited to our existing product line. Our FlexLabSM Service provides you with experience, expertise and the flexibility you require to drive your discovery program. We offer custom protein production for Kinases, Phosphatases and protein substrates and custom cell line development for GPCRs and Ion Channels. In addition, our custom assay development team can design unique assays or extend our current services to fit individual needs.

Cross-Target Safety & Liability Screening


EMD Millipore also offers a unique cross-target service panel for early drug discovery screening against a liability focused functional assay panel of GPCRs, Ion Channels, Kinases and Phosphatases.

Find the real target hiding in your hit list. DaRT Target visualization.
EMD Millipore has introduced the first software application for in-depth visualization of GPCR and kinase profiling data. Our Data Analysis and Report Tool (DART) creates an interactive map of target profiling assay results, enabling you to make faster, more informed decisions in your drug development projects. With the DART Tool, you can:

Securely load previously obtained KinaseProfiler,


GPCRProfiler or AllostericProfiler service data

Map data onto interactive KINome and GPCRome diagrams Visualize % inhibition, % activity and other data correlated
with submitted compounds

Customize normalization and display options Compare activities of up to 4 compounds at once Save or print mapped data

Try the demo: www.millipore.com/visualize

17

Specialty Services: SignalProfiler Services


1.25

Response (Normalized to DMSO)

1.00 0.75 0.50 0.25 0.00

Inhibitor 1.25 1.00 0.75 0.50 0.25 0.00

NEW
Response (Normalized to DMSO)

1.75 1.50 1.25 1.00 0.75 0.50 0.25 0.00

SignalProfiler Service Utilizing MILLIPLEX MAPmate cell signaling kits, SignalProfiler


0.5% DMSO BIRB0796 Sorafenib Getinib Lapatinib Sunitinib Erlotinib Imatinib

Service profiles samples against a panel of 90+ post-translationally modified and total proteins. This Inhibitor service can be used to measure the effectiveness of drug candidates, like kinase inhibitors and GPCR ligands, by measuring changes in signaling cascades in relevant cell backgrounds. In addition, now early signaling events leading to cellular toxicity can be studied to help assess potential compound liabilities in early stages of drug development.

1.75 A549 human lung carcinoma epithelial 1.50 cells were serum starved for 3 hrs, then 1.25 treated with 10 M1.00 the indicated of kinase inhibitors for 1 hr after which 0.75 time the cells were 0.50 treated with 100 ng/mL of EGF for 30 minutes. 0.25 0.00 As anticipated the clinical EGFR inhibitors, Erlotinib, Gefitinib and Lapatinib, prevented EGFR phosphorylation (upper panel) as well Inhibitor as the downstream activation of Erk-1/2 (lower panel). Presumably, Sorafenib, a Raf inhibitor, prevents the activation of Erk-1/2 following EGF treatment by blocking the signal transduction through the Ras/Raf/MAPK pathway. Two independent cell wells were measured for each inhibitor.

Response (Normalized to DMSO)

Flexible Post-Translationally Modified (PTM) Panels


Panel Bioanalyte Phospho ErbB2/Her2 (Tyr) Phospho IRS1 (Tyr) Phospho IGF1R (Tyr) Phospho EGF Rec (Tyr) Phospho c-Met/HGFR (Tyr) Phospho c-Kit (Tyr) Phospho ZAP-70 (Tyr) Phospho PDGF Receptora (Tyr) Phospho Lck (Tyr) Phospho p53 (Ser46) Phospho Histone H2A.X (Ser139) Active Caspase 3 Cleaved PARP (Asp214) Phospho p53 (Ser15) Phospho Bcl-2 (Ser70) Phospho p38/SAPK (Thr180/Tyr182) Phospho JNK/SAPK1 (Thr183/Tyr185) Phospho MKK6 (Ser207/Thr211) Phospho c-Jun (Ser73) Phospho Hsp27 (Ser78) Phospho CREB (Ser 133) Phospho ATF2 (Thr69/71) Phospho PTEN (Ser380) Phospho PRAS40 (Thr246) Phospho STAT2 (Tyr690) Phospho mTOR (Ser2448) Phospho IR (Tyr1162/1163) Phospho Akt/PKB (Thr308), Assay Buffer 1 Phospho STAT3 (Tyr705) Phospho Rsk1 (Ser380) Phospho GSK3b(Ser9) Phospho Bad (Ser112) Phospho STAT6 (Tyr641) Phospho Akt/PKB (Ser473), Assay Buffer 2 Phospho p70 S6 Kinase (Thr412) Phospho MSK1 (Ser212) Phospho ERK 1/2 (Thr185/Tyr187) Phospho STAT5A/B (Tyr694/Tyr699) Phospho STAT1 (Tyr701) Phospho MEK1 (Ser222) Phospho IkBa (Ser32) Phospho STAT3 (Ser727) Related MaPmate Cat Nos. 46-649 46-627 46-647 46-603 46-651 46-619 46-661 46-614 46-616 46-664 46-692 46-604 46-656 46-663 46-660 46-610 46-613 46-672 46-622 46-607 46-631 46-658 46-679 46-681 46-635 46-686 46-688 46-645 46-623 46-611 46-690 46-694 46-633 46-677 46-629 46-674 46-602 46-641 46-655 46-670 46-643 46-624

0.5% DMSO

BIRB0796

Sorafenib

Getinib

Lapatinib

Sunitinib

Erlotinib

Imatinib

0.5% DMSO

BIRB0796

Sorafenib

Getinib

Lapatinib

Pan Tyrosine Panel 2

Response (Normalized to DMSO)

Cell Health Panel 1

Cell Health Panel 2

0.5% DMSO

BIRB0796

Sorafenib

Getinib

Lapatinib

Sunitinib

Erlotinib

Imatinib

Cell Stress Panel

Inhibitor

FEATURES

Cell Metabolism Panel

Inspection of common intracellular signal transduction cascades and networks Use cells that are relevant for your research Cell-based assays for compound efficacy and liability Run by experts in compound selectivity-profiling

General Cell Signaling Panel 1

General Cell Signaling Panel 2

18

Sunitinib

Erlotinib

Pan Tyrosine Panel 1

Imatinib

Fixed Post-Translationally Modified (PTM) Panels


Panel Bioanalyte Related MaPmate Cat Nos. Phospho CREB (Ser133) Phospho ERK 1/2 (Thr185/Tyr187) Phospho Lck (Tyr) Human T Cell Receptor - 7 plex Panel Phospho ZAP-70 (Tyr) Phospho Lat (Tyr) Phospho CD3(Tyr) Phospho Syk (Tyr) Phospho Lck (Tyr) Phospho Src (Tyr) Phospho Fyn (Tyr) Phospho Blk (Tyr) Human SRC Family Kinase 8 Plex Panel Phospho Fgr (Tyr) Phospho Hck (Tyr) Phospho Lyn (Tyr) Phospho Yes (Tyr) Phospho Akt/PKB (Ser473), Assay Buffer 2 Phospho GSK3b (Ser9) Phospho IR (Tyr1162/1163) Phospho mTOR (Ser2448) Phospho p70 S6 Kinase (Thr412) Akt / mTOR Panel - 11 plex Phospho PTEN (Ser380) Phospho RPS6 (Ser235/Ser236) Phospho TSC2 (Ser939) Phospho IRS (Ser312) Phospho GSK3a(Ser21) Phospho IGF1R (Tyr1135/Tyr1136) Phospho ATF2 (Thr69/71) Phospho c-Jun (Ser73) Phospho ERK 1/2 (Thr185/Tyr187) Phospho Hsp27 (Ser78) Phospho JNK/SAPK1 (Thr183/Tyr185) Human MAPK/SAPK - 10 Plex Panel Phospho MEK1 (Ser222) Phospho MSK1 (Ser212) Phospho p38/SAPK (Thr180/Tyr182) Phospho p53 (Ser15) Phospho STAT1 (Tyr701) Phospho CREB (Ser133) Phospho ERK 1/2 (Thr185/Tyr187) Phospho Ika (Ser32) Phospho JNK/SAPK1 (Thr183/Tyr185) Multi-pathway - 8 Plex Panel Phospho p38/SAPK (Thr180/Tyr182) Phospho p70 S6 Kinase (Thr412) Phospho STAT3 (Ser727) Phospho STAT5A/B (Tyr694/Tyr699)

Flexible Total Panels


Panel Bioanalyte Total Lck Total IRS1 Total IGF1R Total ErbB2/Her2 Total EGF Receptor Total c-Met/HGFR Total PRAS40 Total Akt/PKB, Assay Buffer 1 Total GSK3b Total Bad Total p53 Total MSK1 Total p21 Total IR Total c-Kit Total p38 Total MKK6 Total JNK Total PTEN Total Hsp27 Total STAT2 Total mTOR Total CREB Total Bcl-2 Total ATF2 Total Human GAPDH Total ERK 1/2 Total Akt/PKB, Assay Buffer 2 Total STAT1 Total IkBa Total STAT3 Total p70 S6 Kinase Total MEK1 Total HIF1a Related MaPmate Cat Nos. 46-617 46-628 46-646 46-648 46-606 46-650 46-680 46-605 46-689 46-695 46-662 46-673 46-621 46-687 46-620 46-612 46-671 46-618 46-678 46-608 46-634 46-685 46-632 46-659 46-657 46-667 46-609 46-675 46-654 46-644 46-625 46-630 46-669 46-665

48-690

Total Panel A (Tyr related)

Total Panel B (Gen Signal related)

48-650 Total Panel C

Total Panel D (Stress related) 48-611

Total Panel E (General sig 2) 48-660

Order online
Products: www.millipore.com/mapmates Services: www.millipore.com/portal
48-680

Download the appropriate service request form at EMD Millipores Drug Discovery and Development Service Portal.
19

To Place an Order or Receive Technical Assistance


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