. 13/01/53 Zz .. zZ
Intro.
automated analyzers
save time & staffs
Definition
Automation
Mechaniczl device ~ manual
Development
semi-automation autopipette / dispenser /
mixer / diluter / spectrophotometer
Fully-automation
1957 Dr.Leonard Skeggs Automate
AutoAnalyzer (AA)
Automate
Terminology ()
Complete(full) automation :
step ,
mix,incubate,,report
Partial(semi-) automation :
Single-channel : (1 ) 1 test
Multi-channal : () 1 test
Non-selective : 1 test
test sample
electrolyze, blood gas
Dead volume : sample volume
Dwell time :
sample
Stat testing :
Cost : ..
std.,control,
Analyzer configuration :
Open system :
Closed system :
..
Centralized testing : /
lab s ample
Point-of-care testing(POCT) :
test b
edside
POCT
.. lab
..
whole blood
, calibrate
..
automated : 2
Continuous-flow ( slide 12 6)
test
test
test
sample
.. 1 = 1 test
heating incubate
Discrete-flow (
slide 1 3 7)
sample
Discrete analyzer 2
..
( manual automate slide 1 7 9)
Sampler
Mixer
(mixing coil)
(mechanical mixer)
.. specimen
hemolysis
human
messenger
reactants
heat block
water bath
Measurement approaches
photometry
Signal processing, data handling, process control
computer
..
sample test
throughput .. ..
Stat capability .. stat
Method .. basic?
photometry
..
test....
..
..
..
Trend
..fully automated
Maintenance
Daily :
Weekly :
Monthly :
Yearly :
leaflet.. + autoanalyzer..
Beckman CX4
..
.. endpoint kinetic
.. IU/L, mg/dl, g/L
.. positive( product ),
negative( substrate ) leaflet
Cal factor
calibrater conc. calibrator
linear
cal time limit cal factor calibrate
2
1wavelength 2
wavelength
test
read time blank(
OD. ) reaction(
)
lower & upper limit
Absorbance limit : blank( ) &
reaction .. lower & high limit
Substrate depletion
kinetic assay
!!
..
.. 555