Biology
IB
Research Question
How does the amount of time affect how much gas is released when 10ml of catalase is dropped onto yeast?
Hypothesis
I think the optimal amount of time would 1 minute because after one minute the amount of gas released would significantly decrease.
Alan Jones
Biology
IB
Dependent Variable
My dependent variable will be the gas (mostly oxygen) that is released from the yeast and it will depend on the amount of time I leave it in the bowl for. To measure this, the gas syringe will be at 0cm and then I will measure the difference once the time has elapsed. This will be controlled by using a gas syringe.
Controlled Variables
Controlled variable Amount of hydrogen peroxide Amount of yeast Same gas syringe How will it be controlled? Will be measured before use. Will be measured before use. Wont change gas syringe. What effect does it have? Produce unfair results. Produce unfair results. Could lead to uncertainties
Apparatus
Stopwatch Knife Gas syringe with bung 300ml of catalase Bowl for gas syringe 500 grams of yeast Small pipette 250ml beaker Weighing boats
The Method
1. Place 20grams of yeast inside beaker. I use 20 grams because it is a sufficient amount to produce required results. 2. Take about 200ml of hydrogen peroxide. 3. Take 10ml of hydrogen peroxide into the pipette and have it ready to be applied to the yeast. 4. Try to apply to the centre as much as possible. 5. Once applied, seal the beaker with the gas syringe (making sure its at 0 before being placed on). 6. Time for 30 seconds.
Alan Jones
Biology
IB
7. Once 30 seconds have elapsed, see how many cm of difference there is on the gas syringe and make a note of it. 8. Once written down, take off the bung and clean out the bowl (or whatever else has been used). 9. Repeat this step with waiting 30 seconds 4 more times. 10. Once completed, move onto the other IVs and do those 5 times each.
Results Table
30 Time elapsed (seconds) 60 120 240 360 Result 1 Result 2 Result 3 Result 4 Result 5
With this data the research question can be addressed. The results will be presented using line graphs and processed results tables. This will be done via placing the data into Microsoft Office Excel. 5 suitable increments are used for the IV.
Bibliography
No external sources were used.