Epidemiology
Features of
Acinetobacter
baumannii
Colonizing
Respiratory Tracts
of ICU Patients
2012
THE INTERNATIONAL ARABIC JOURNAL
OF ANTIMICROBIAL AGENTS
2Department of Internal
Medicine-Jordan University
Hospital, Faculty of Medicine,
The Jordan University, Amman,
Jordan
Correspondence:
Prof. Asem A Shehabi
Department of PathologyMicrobiology, Faculty of Medicine,
The Jordan
University, 11942 Amman, Jordan
ashehabi@ju.edu.jo
Abstract
Background: A. baumannii is becoming a common opportunistic and nosocomial
pathogen in ICUs worldwide. Few studies have investigated the epidemiology of A.
baumannii isolates colonizing the respiratory tract of hospitalized patients in ICUs.
Results: Two third of hospitalized patients (63/93; 67.7 %) have their respiratory
tract colonized with A. baumannii, and significant risk factors associated with
colonization were longer ICU stay, use of mechanical ventilators and antibiotic
treatment with carbapenems. All A. baumannii isolates were multidrug resistant
(MRAB) and possessed blaOXA-51-like gene; in addition, 73% and 19% of the
isolates harbored blaOXA-23-like and blaOXA-24-like genes, respectively. All isolates were negative for MBL-encoding gene (blaIMP-1) and only two isolates (3%)
harbored (blaVIM-2) gene. The genetic similarity of A. baumannii isolates using
ERICs-PCR and constructed dendrogram showed 2 major genotype clusters of
genetically related isolates.
Conclusion: This study shows that few genotypes of MRAB are often colonizing
the respiratory tract of hospitalized patients in ICUs, and colonization was rarely
associated with blood sepsis and mortality.
Introduction
Acinetobacter baumannii has emerged as an important nosocomial opportunistic pathogen in intensive care units (ICUs)
worldwide (1-4). The organisms ability to survive for extended periods on inanimate surfaces and by the presence of humidity conditions allows its existence as a potential pathogen
in the hospital environment and its numerous spread among
hospitalized patients (4-5).
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A. baumannii causes generally ventilator-associated pneumonia, septicemia, and surgical site infection in hospitalized
patients (3). It has been also reported that extensive usage of
broad-spectrum antibiotics in hospitals was associated with
increased incidence of multidrug resistance A. baumannii
(MRAB) rates, specifically to carbapenems (4).
Additional risk factors for the acquisition of MRAB infection
or colonization included longer hospitalization stay, admis-
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Methods
Patients
A total of 142 respiratory samples (bronchoalveolar lavage,
tracheal aspirate and sputum) were consecutively collected
from 93 patients admitted to intensive care units (ICUs), Jordan University Hospital (JUH) over a 10-month period (May
2009-Febreuary 2010). For all patients; the name, age, sex,
clinical diagnosis, type of antibiotics administered, mechanical ventilation, length of ICU stay, presence of tracheotomy,
developing blood sepsis and mortality were recorded. This
study was proved by Independent Research Ethics Review
(IRB) no. L29/2009, Jordan University Hospital.
Sample processing
All collected samples were cultured on blood agar, CLED agar,
and minimal-salt agar supplemented with 1% acetate (MSA)
for detection of colonization with Acinetobacter spp. All suspected Acinetobacter growing colonies were first identified
by conventional techniques and confirmed later as A. baumannii by Rapid NF plus system, Remel Kit (USA). Only one
positive culture was included for each patient, and all isolates
were stored in brain-heart infusion agar plus 15% glycerol at
-70 C until tested.
Statistical analysis
All data were analyzed using the computerized statistical
analysis (SPSS, version 16). p value <0.05 was considered
statistically significant.
Results
A total of 63/93 (67.7 %) of ICU patients has their respiratory
tract colonized by A. baumannii. Only one patient died following developing blood sepsis due to infection / colonization
with A. baumannii during the study period. Demographic
data of patients show the common risk factors associated
with A. baumannii colonization/infection (Table 1). The antimicrobial susceptibility pattern of 64 A. baumannii isolates
including one blood isolates from the same patient is shown
in Table 2. The susceptibility results for 11 tested agents
ranged between 63% and 100%, and 58/64 (89%) of the
isolates were multidrug resistant to at least for 6 tested antimicrobial agents. There was no resistance observed to both
tigecycline and colistin. The MIC90 and MIC range to amikacin, imipenem, colistin and tigecycline are shown in Table
2. The distribution of -lactamases genes among 64 A. baumannii isolates is shown in Table 3. All isolates possessed the
encoding gene for an intrinsic OXA-51 carbapenemase, 73%
were positive for blaOXA-23-like gene, and 19% were positive for blaOXA-24-like gene. None of the isolates harbored
blaOXA-58-like or MBL-encoding gene (blaIMP-1) and Only
two isolates(3%) harbored (blaVIM-2) gene.Two major clus-
2012
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Table 1.Major demographic characteristics of 93 adult patients admitted to ICUs and examined for respiratory tract colonization with A .baumannii.
Variables
P-value
Total of patients*
63 (67.7)
30 (32.3)
<0.001
0.344
Gender
Male
36 (72.0)
14 (28.0)
Female
27 (62.8)
16 (37.2)
Mean age
60.17 18.993
58.119.971
0.629
7.51 1.595
2.73 2.37
< 0.001
1-3 days
1 (3.7)
26 (96.3)
4-8 days
56 (94.9)
3 (5.1)
> 8 days
6 (85.7)
1 (14.3)
< 0.001
53 (81.5)
12 (18.5)
No
10 (35.7)
18 (64.3)
60 (84.5)
11 (15.5)
< 0.001
Mechanical ventilation
Yes
<0.001
No
3 (13.6)
19 (86.4)
5.02 1.17
1.36 0.67
<0.001
Yes
11 (78.6)
3 (21.4)
0.347
No
52 (65.8)
27 (34.2)
Tracheostomy
*All underlying diseases in patients were not significant for respiratory tract colonization with A.baumanni, and only one male patient was colonized and developed fatal blood sepsis with the same strain.
**Most patients (77%) were treated only with carbapenems during their stay in ICUs.
No. (%)
Resistant isolates*
MIC90 (mg/L)
MIC-range (mg/L)
Ceftazidime (Caz)
64 (100)
ND **
ND
Ertapenem (Etp)
64 (100)
ND
ND
Meropenem (Mem)
64 (100)
ND
ND
Piperacillin/Tazobactam
(Ptz)
63 (98)
ND
ND
Aztreonam (Atm)
63 (98)
ND
ND
Gentamicin (Gm)
60 (94)
ND
ND
Ciprofloxacin
58 (91)
ND
ND
Amikacin (Ak)
47 (73)
13.6
1-16
Imipenem (Imi)
40 (63)
12
0.19-12
Colistin (Co)
0 (0)
0.19-1
Tigecycline (Tgc)
0 (0)
0.38-4
* ND = Not done.
2012
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Class D
Type of
-lactamase
Prevalence (%)
IMP
0 (0)
VIM
2 (3)
OXA-51
64 (100)
OXA-23
47(73)
OXA-24
12 (19)
OXA-58
0 (0)
1st
genotype
Size of common
DNA fragments(bp)
No. of
isolates
500-1500
23
600-2000
16
200-3000
700-1500
18
2nd
Discussion
This study found that about two third (67.7%) of hospitalized patients in various ICUs at JUH in Amman, have their
respiratory tract colonized with A. baumannii. Major demographic characters of patients were incorporated for multivariate analysis which revealed that antibiotics treatment,
the longer duration of mechanical ventilation and stay in the
ICUs (P<0.05) were significant independent risk factors for
colonization with A. baumannii.
In particular, antibiotic treatment with carbapenems has been
shown to be mostly associated with respiratory tract coloni-
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2012
THE INTERNATIONAL ARABIC JOURNAL
OF ANTIMICROBIAL AGENTS
iMedPub Journals
2012
THE INTERNATIONAL ARABIC JOURNAL
OF ANTIMICROBIAL AGENTS
Acknowledgements
This study was partly supported by grant (46/2008-2009)
from Deanship of Research, University of Jordan, Amman,
Jordan.
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2012
THE INTERNATIONAL ARABIC JOURNAL
OF ANTIMICROBIAL AGENTS
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