OOSIGHT IMAGING SYSTEMS

See what youve been missing.

Oosight. The non-invasive imaging system that reveals critical structures

in the oocyte to improve egg grading and enable new discoveries.
Adding Oosight to your lab can improve success by offering you a quantitative and reproducible method to measure biological disruption in either fresh or previously frozen oocytes. You can now select against oocytes for ICSI and embryos for implantation, and use the system to dramatically improve enucleation efficiency. Understanding the oocyte is critical to understanding the foundation of embryogenesis. Studies show that a disrupted spindle apparatus or a weakened zona pellucida in the oocyte have yielded lower pregnancy rates. In fact, pregnancy is 8-times more likely when the inner zona pellucida is well-ordered. 1
Immature Oocyte with Extruded PB

Shen Y, et al. High magnitude of light retardation by the zona pellucida is associated with conception cycles. Human Reproduction, 2005 Jun;20(6):1596-606. Photography credits Front cover Retardance pseudocolor image of MII human oocyte Back cover Retardance grayscale image of MII human oocyte Before/After image above spec chart courtesy of RMA NJ

Oocyte screening can make all the difference.

Oosight enables you to determine which subpopulation of oocytes are at high risk for producing chromosomally abnormal embryos. One in approximately 20 cycles contains oocytes that are immature but are nevertheless falsely labeled MII using conventional imaging techniques. Oosight can prevent the potentially damaging effects that result from injecting immature oocytes. The system can also help screen for oocytes with highly disrupted spindles, such as those that are multipolar.

Orientation Color Map Oosight outperforms other systems.

before after

No other contrast-enhancement technique delivers the performance of Oosight. With its unprecedented resolution and calibrated setup, Oosight provides the reproducibility and sensitivity Orientation Vector Map required of a grading routine, as well as the speed needed for micromanipulation.

Retardance Color Map

microscope not included

Its an easy add-on.

The Oosight system integrates seamlessly onto your ICSI workstation. Now with foot-pedal controls and automated spindle and zona tools, measuring each oocyte is a simple 2-step process. Snap an image and then press a button to report the data. Meaningful retardance and orientation data are organized into an intuitive, exportable report. Its really that simple.
From the conventional Oosight image of human MII oocyte constrast before image, taken just prior to ICSI. The spindle structures nicely the apparatus issuch asbarrel shaped spindle and layers of and the three multiple the zona layers of the zona pellucida are all visible. pellucida remain invisible.

Improve the efficiency of oocyte cryopreservation.

Whenever a clinical application is known to negativelyFinderTMthe state Automated Spindle alter of the biological material being used, it is imperative that checks and balances are in place to monitor the extent of that change. Oosight can help do this for all cryopreserved oocytes by providing a method that helps ensure Inner-zona FinderTM Automated that vital structures in the oocyte are reformed to their original, pre-frozen state.
Images to the right display the various analysis tools Contact us at 1-800-383-7924 or at www.cri-inc.com/oosight to learn more in the Oosight software.

about Oosight or to request a quotation of this breakthrough system.

Oocyte screening can make all the difference.

Oosight enables you to determine which subpopulation of oocytes are at high risk for producing chromosomally abnormal embryos. One in approximately 20 cycles contains oocytes that are immature but are nevertheless falsely labeled MII using conventional imaging techniques. Oosight can prevent the potentially damaging effects that result from injecting immature oocytes. The system can also help screen for oocytes with highly disrupted spindles, such as those that are multipolar.

Oosight outperforms other systems.

No other contrast-enhancement technique delivers the performance of Oosight. With its unprecedented resolution and calibrated setup, Oosight provides the reproducibility and sensitivity required of a grading routine, as well as the speed needed for micromanipulation.

after

microscope not included

Its an easy add-on.

The Oosight system integrates seamlessly onto your ICSI workstation. Now with foot-pedal controls and automated spindle and zona tools, measuring each oocyte is a simple 2-step process. Snap an image and then press a button to report the data. Meaningful retardance and orientation data are organized into an intuitive, exportable report. Its really that simple.
Oosight image of human MII oocyte taken just prior to ICSI. The spindle apparatus is nicely barrel shaped and the three layers of the zona pellucida are all visible.

Improve the efficiency of oocyte cryopreservation.

Whenever a clinical application is known to negatively alter the state of the biological material being used, it is imperative that checks and balances are in place to monitor the extent of that change. Oosight can help do this for all cryopreserved oocytes by providing a method that helps ensure that vital structures in the oocyte are reformed to their original, pre-frozen state. Contact us at 1-800-383-7924 or at www.cri-inc.com/oosight to learn more about Oosight or to request a quotation of this breakthrough system.

normal

abnormal

On the left, human MII oocyte has a normal barrel-shaped spindle (azimuth skew from barrel shape is 5.12) . On the right, this human MII oocyte has an abnormal tri-polar spindle (azimuth skew from barrel shape is 49.4). Inner zona and spindle retardances are not shown, but these data are provided by the Oosight system as well.

Oosight Imaging Systems

Model
LC Compensator CPIF CCD Camera C-Mount for Camera Image Capture Software Automated Spindle FinderTM* Automated Zona FinderTM* Reporting Tools Movie Capture
*The Spindle And Zona Finder are tools in the Oosight software used to automatically find and report on spindle and zona metrics, including mean retardance and orientation offset. NOTE: The Oosight system is compatible with most research-grade microscopes and laser systems. Glass bottom dishes are required.

Basic

Meta

Because spindle screening can improve efficiency of gamete selection, it should be considered when choosing eggs for freezing or fertilization by ICSI. Dr. N Noyes, NYU IVF Program

About CRi
Cambridge Research & Instrumentation, Inc (CRi) is a global leader in biomedical imaging dedicated to providing comprehensive solutions that enable our customers to perform cutting-edge research and provide better healthcare. CRi technology helps extract physiologically and medically significant information from biological and clinical samples with intact morphological and biochemical context in tissues and organisms. With over 80 patents pending and issued, CRis award-winning innovations are being utilized around the world.

Your local CRi agent or distributor is:

Cambridge Research & Instrumentation, Inc. CRi 35-B Cabot Road Woburn, MA 01801 USA E-mail: sales@cri-inc.com Website: www.cri-inc.com Toll-Free (USA): 1-800-383-7924 Direct (Worldwide): +1-781-935-9099

Notice to purchaser: The Oosight Imaging System is meant to be used for research purposes only.