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Ameliox Goodbye wrinkles, goodbye glycation

Ameliox Goodbye wrinkles, goodbye glycation

Carnosine and Powerful Antioxidants to Prevent Glycation Ameliox is a liposomal preparation combining carnosine, an antioxidant and anti-glycation substance with 2 powerful oil-soluble antioxidants (silymarin and tocopherol). Glycation is a slow naturally-occurring process which affects the structure and the activity of proteins. In the skin, it results in the stiffening of the dermis proteins (collagen and elastin), leading to a loss of elasticity and to premature aging. Oxidative stress factors and notably lipid peroxidation accelerate the glycation process. Ameliox is able to inhibit lipid peroxidation and so, prevents and slows down this deleterious process. In a clinical study, Ameliox was shown to: increase skin firmness, smoothness and hydration reduce the depth of wrinkles by 27 % in the crows feet area and by 24 % on the forehead after 1 month of treatment. Thus, Ameliox can delay the glycation process and prevent oxidative aging. Claim Ideas for Ameliox Maintains skin elasticity by fighting glycation Reduces wrinkles and lines Increases skin firmness Protects skin cells from oxidative aging Applications Anti-wrinkle for face, dcollet and eye contour Protective formulas Advanced anti-aging and anti-glycation products Formulating with Ameliox Recommended use level: 1 5 % Incorporation: For cold processes, dissolve Ameliox into the aqueous phase. In cold / hot processes, add during the cooling phase below 60C. Thermostability: Temperatures of up to 60C for a short time do not affect the stability of Ameliox. INCI / CTFA-Declaration Ameliox (standard version): Lecithin (and) Carnosine (and) Tocopherol (and) Silybum Marianum Fruit Extract (and) Glycerin (and) Alcohol (and) Aqua / Water Ameliox oA (alcohol-free version): Lecithin (and) Carnosine (and) Tocopherol (and) Silybum Marianum Fruit Extract (and) Glycerin (and) Phenoxyethanol (and) Aqua / Water

September 2010

Oxidative Stress Accelerates the glycation process

Glycation, a Naturally-Occurring Process Glycation (also called Maillard or "browning" reaction) is a slow process where reducing sugar molecules react with free amino groups of proteins leading to the formation of irreversible cross-linked molecules called AGE (advanced glycation end-products). This process occurs naturally in most foods during heating and also, but more slowly, in living organisms at body temperature. Glycation is a Major Contributor to Aging Glycation is deleterious to proteins as it leads to the detrimental and irreversible modification of their structure and activity. In skin, glycation results in the formation of rigid bridges between structural proteins such as collagen and elastin: making the dermis proteins stiffer and more brittle affecting their interactions with other cells. This results in: a loss of elasticity and suppleness of the skin the appearance of wrinkles the yellowing of the skin. Oxidative Stress Promotes the Glycation Process Several factors promote the glycation process: high glucose levels aging (notably after 35) oxidative stress such as UV-induced lipid peroxidation which leads to the formation of malondialdehyde, a reactive compound. In their turn, the AGE formed bind to fibroblasts to release free radicals and reactive oxygen species (ROS), leading to a vicious circle.

Formation and Effects of AGE

UV

UV

Aging

Malondialdehyde Malondialdehyde High glucose Aging level High glucose level

Glycation Proteins Glycation AGE Proteins (collagen) (collagen)

Loss of skin elasticity Loss of skin elasticity Damaged collagen Damaged (structure, function) collagen (structure, function) Wrinkles Wrinkles

AGE

Yellowing of the skin Yellowing of the skin

Ameliox Preventing the glycation process with carnosine and powerful antioxidants
Carnosine, the Anti-Glycation Factor Carnosine, a dipeptide which occurs naturally in our body, is well-known for its anti-glycation activity. Carnosine inhibits the cross-linking of proteins induced by reducing sugars and reactive aldehydes such as malondialdehyde. As a glycation target, carnosine "competes" with the skin proteins and therefore, protects collagen and elastin fibers. In contrast to AGE, the resulting glycated carnosine is not deleterious and not toxic. Powerful Antioxidants Reinforce Carnosines Effect Silymarin is the major active substance of milk thistle, a flowering plant of the daisy family. This polyphenol supports physiological antioxidants and radical scavengers such as glutathione and superoxide dismutase and is also able to capture and neutralize free radicals. It inhibits lipid peroxidation and inflammation as well as the release of histamine. Tocopherol is naturally present in the skin being the major lipid-soluble antioxidant. Located in cellular membranes, it protects skin lipids against peroxidation and maintains the barrier function. The liposomal form increases the bioavailability of all compounds thanks to the small size of the liposomes and their high affinity to the stratum corneum. This results in an enhanced and long-lasting activity.

Carnosine Protects the Dermal Proteins from Glycation

Cross-linked collagen bundles (AGE)

Glycation

Collagen

Free sugar +Carnosine

Collagen bres are protected and stay elastic

Ameliox Study results

Malondialdehyde Promotes the Glycation Process Malondialdehyde (MDA) is a reactive aldehydic molecule which accelerates the glycation process. It results from the degradation of polyunsaturated fatty acids by ROS which are mostly generated by UVA. Malondialdehyde is thus a marker for oxidative stress. Malondialdehyde damages proteins by oxidizing them and by modifying their structure and function leading to the formation of AGE. Antioxidant and Anti-Glycation Effects of Ameliox Different concentrations of Ameliox were added to borage oil that was encapsulated in a nano-emulsion. This oil is rich in polyunsaturated fatty acids and thus sensitive to UV. Then, the nano-emulsions were radiated with UVA light (2 hours at 500 Watt) leading to the formation of lipid peroxides. The concentration of lipid peroxides was measured using the thiobarbituric acid assay. This technique is based on the transformation of lipid peroxides into malondialdehyde which forms a red complex with thiobarbituric acid. The absorbance of this complex is measured at 532 nm using a spectrophotometer. Results showed that Ameliox clearly inhibited the lipid peroxidation and the formation of MDA in a dose-dependent manner indicating an antioxidant and anti-glycation effect.

Formation and Effects of MDA

Inhibition of MDA Formation

UV-A Lipids Silymarin and tocopherol

100

Peroxidation

Inhibition of MDA formation compared to control in %

80

60

Malondialdehyde (MDA) Carnosine

40

20

0
AGE formation

0.5

2 Concentration of Ameliox in %

S-306 / Mibelle AG Biochemistry 2010

S-306 / Mibelle AG Biochemistry 2010

A Younger-Looking Skin A cream containing 2 % Ameliox was applied twice daily for 28 days to the inner side of the forearm of 20 women aged from 38 to 65. Biomechanical properties of the skin were evaluated by determining the skin firmness, hydration and smoothness using respectively a cutometer, a corneometer and the PRIMOS system. Results showed that Ameliox improved all parameters after 28 days of treatment.

Anti-Wrinkle Effect A cream containing 2 % Ameliox was applied twice daily for 28 days to the crows feet and to the forehead of 20 women aged from 38 to 65. Wrinkle depth was evaluated using the PRIMOS system. Results showed that: Ameliox decreased the depth of wrinkles already after 14 days of treatment Ameliox reduced the depth of winkles by 27 % in the crows feet area and by 24 % on the forehead after 28 days of treatment.

Increase of Smoothness, Firmness and Hydration

Untreated 25 Increase compared to initial conditions after 28 days in % Emulsion with 2 % Ameliox

Reduction of Wrinkle Depth

After 14 days 30 Decrease of wrinkle depth compared to initial conditions in % After 28 days

20 15 10 5 0 -5 -10 Hydration Firmness Smoothness

20

10

0 Forehead Crow's feet

S-061 / Mibelle AG Biochemistry 2010

S-061 / Mibelle AG Biochemistry 2010

Mibelle AG Biochemistry, 5033 Buchs/Switzerland, Phone +41 (0)62 836 17 31, info@mibellebiochemistry.com, www.mibellebiochemistry.com

Ameliox Goodbye wrinkles, goodbye glycation

Claim Ideas for Ameliox Maintains skin elasticity by fighting glycation Reduces wrinkles and lines Increases skin firmness Protects skin cells from oxidative aging Applications Anti-wrinkle for face, dcollet and eye contour Protective formulas Advanced anti-aging and anti-glycation products Marketing Benefits Ameliox impacts 3 main biochemical reactions that manifest the signs of skin aging: the formation of free radicals, the lipid peroxidation and the development of advanced glycation end-products (AGE) With liposomal carnosine a dipetide that inhibits the formation of AGE Preservative-free

The information contained in this publication is provided in good faith and is based on our current knowledge. No legally binding promise or warranty regarding the suitability of our products for any specific use is made. Claim ideas are offered solely for your consideration, investigation and verification. Mibelle Biochemistry will not assume any expressed or implied liability in connection with any use of this information.