No.

PENELITI Feliatra, Irwan Efendi Edwar Suryadi

MASALAH Isolasi dan Identifikasi Bakteri Probiotik dari Ikan Kerapu Macan (Ephinephelus fuscogatus) dalam Upaya Efisiensi Pakan Ikan

Deskripsi Subjek
Laboratorium Mikrobiologi Laut, Faperika, Universitas Riau, Pekanbaru

Instrumen
inkubator, autoklaf,erlenmeyer, pemanas, alumunium foil, lampu Bunsen, cawan petri, neracaOhauss dengan ketelitian 0,1 g, gelas ukur, tabung reaksi, kapas, motor steril, pipet (0,1, 1,0 dan 10ml) dan pro, janke dan kunkel, mikroskop binokuler,gelas objek, glass speader, jarum oase dan colonycounter

Metode & Bahan

media agar non selektif TSA (Tryptone Soya Agar), Difco, MR-VP Broth, reagen methyl red, TSI (Triple Sugar Iron) agar, bahan untuk uji pewarnaan Gram (crystal violet, lugol iodine, safranin, etil alkohol 95%, dan aquades), hydrogen peroksida (H2O2), larutan naftol (1 g per 100 ml etil alkohol) larutan phenilendiamin (1 g per 100 ml air destilasi). Peralatan perikanan seperti ember, lambit dan peralatan mikrobiologi dan peralatan untuk analisavirus KHV. Wadah yang

HASIL TEMUAN
Berdasarkan hasil penelitian yang dilakukan,diperoleh bahwa bakteri yang diisolasi dari usus dan lambung ikan kerapu macan dapat tumbuh dan berkembang pada media kultur TSA dengan pH 2, yang merupakan indikator utama bakteri probiotik. Koloni bakteri yang tumbuh pada media terdapatdalam berbagai macam bentuk, warna, tepian, ukuran, dan permukaan koloni (elevasi) yang berbeda.

E. Mudjiutami, Ciptoroso, Z. Zainun, Sumarjo, Rahmat

PEMANFAATAN IMMUNOSTIMULAN UNTUK PENGENDALIAN PENYAKIT PADA IKAN MAS

KJA Cirata, Cianjur Jawa Barat

Bahan immunostimulan (Chromium yeast) serta bahan analisa mikrobiologi dan analisa virus KHV. Ikan uji yang digunakan berupa ikan mas bebas KHV yang berukuran 10 gram sebanyak 12.000 ekor.

Hasil penelitian menunjukkan Cr-yeast memberikan respon positif terhadap sintasan pemeliharaan maupun sintasan setelah uji tantang. Sintasan pemeliharaan ikan mas untuk masing-masing perlakuan A, B, C dan D secara berurutan adalah: 77.70%, 74.50%, 67.80% dan 54.30%. Sedangkan sintasan setelah ujitantang berturut-turut adalah: 76.66%, 63.33%, 50.00%

digunakan adalah karamba jaring apung yang berukuran 3x3x3 m3 sebanyak 12 buah.

dan 20.00%. Respon tanggap kebal terhadap KHV juga ditunjukkan melalui gambaran darah ikan mas uji.

Saldyansah Effendy Alexander Rantetondok & Akbar Tahir

Sering terjadinya kegagalan dalam budidaya akibat mewabahnya penyakit berupa virus makanya perlu dilakukanPengkajian terhadap imunostimulan terutama pada usaha perbenihan dan selayaknya diarahkan pada cara pemberian,hubungan antara dosis dan respon, serta evaluasi terhadap stimulasi terhadap sistem imun.

Panti benih skala rumah tangga di Desa Aeng Batu Kecamatan Galesong Utara Kabupaten Takalar

Sentryfugasi pada 6.000 rpm (Hettich-EBA), Tabung reaksi tertutup volume 13 ml (Iwaki, Pyrex Japan), Sonikator (VWR model 75D, 50-60 Hz, VWR Intl USA) benur PL 10 serta ekstrak ragi roti sebagai imunostimulan. BiakanSaccharomycesce

Pengamatan di Laboratorium
Balai Budidaya Air Payau Takalar

New Caledonia is located 1500 km east of the East Australian coast at the border of the Melanesian arc in the Southwest Pacific Ocean.

Electrofshing, Hand net, Binocular magnifier, stereomicroscope, camere, computer, ruler

Observation, Laboratory tested. Sampel : S. Cantharus

Dari hasil penelitian menunjukkan bahwa hemocyanin Akan m pada memberi respon pada juwana (Litopenaeus vannamei) yang diberi pakan mengandung -glucan. Pening-katan tersebut diduga berkaitan erat dengan mekanisme stimulasi -glucan pada sistem kekebalan tubuh udang. Menurut Johansson et al. (2000) dan Lopez et al. (2003), belum ada persyaratan mengenai jumlah glucan yang dapat meningkatkan sistem kekebalan tubuh udang. Sistem tersebut dapat saja me-ningkatkan jumlah haemosit hanya karena jumlah glucanyang sangat sedikit, misalnya dalampicogram.Peningkatan HC yang cukup ekstrem.Peningkatan tersebut diduga merupakanrespon dari munculnya peroxinectin.

yang dihasilkan oleh degranulasi

The traditional counting of annual growth zones on thick otoliths of gadiform fish requires special processing methods and is for that reason difficult, expensive and timeconsuming in the southeastern Adriatic Sea (Elafiti Islands) Trammel net, ruler, weight measurer, microscopic, labelled envelope, digital camera, computer south western Portugal; Sesimbra, Sagres and Faro Trammel net, gill net, ruler, weight measurer, plastic forceps, lint-free paper, microcentrifuge tube, ultrasonic cleaner, H2O2, HNO3, computer at three
Haemocyte,

Observastion, laboratory test.

The common twobanded sea bream (Diplodus vulgaris) is an important fish in the marine ecosystems of the NW Atlantic and Mediterranean. In southern Portugal it is a major fishery resource being targeted mainly by the artisanal fleets Fish ecologists have used geochemical values in otoliths to examine habitat use, migration, and population connectivity for decades. However, it remains difficult to determine an unambiguous dietary

significantly higher for the Sagres samples.

These results are congruent both for the endemic species, S. aiensis and for the cosmopolitan species. Finally, we found that, for S. lagocephalus, the cosmopolitan species, New Caledonian samples are close to western Vanuatu samples, the latter two being well differentiated from the eastern central Vanuatu samples. Observation, laboratory tested. Sampel Phycis phycis

7.

d13C signature from bulk analysis of otolith.

locations along a crossshelf transect from Al Lith, Saudi Arabia, in the Red Sea

(Gadidae) All measured otolith morphometric parameters (length, width and mass) were linear with fish age. The linear model explained between 61.3% and 69.0% of the variation in age. The most precise age estimations of analysed population were obtained from the otolith mass, followed by the otolith width and length Comparison of the otolith core chemistry utilised Sr/Ca, Ba/Ca, Mn/Ca and Mg/Ca and Zn/Ca. LDFA for the otolith core chemistry also achieved accurate classification for samples from Sesimbra (73%), but there was high overlap of otolith chemistry between samples from Faro and Sagres (47 and 43% classification accuracy respectively)

A massive fish kill of approximately 50 tons of flathead grey mullet (Mugil cephalus) occurred in August 2006 in the Tanshui River, northern Taiwan.

Ruler, water, stored dry, freezer, boat, computer, microscope, ultrapure water, merchantekmicroMill, glass reaction

Observation, Laboratory tested. Sampel Diplodus vulgaris

8.

in the Tanshui River, northern Taiwan

Herring along the west coast of the British Isles are managed and assessed as a series of discrete stocks. The relationship between the spawning components, mixed (feeding) aggregations, and juveniles in nursery areas for these stocks was modelled by discriminant

Ruler, termometer, weighter, de-ionized water, air dryer, H2O2, spectrometer, laser, computer, microscope

Observation, laboratory tested

along the west coast of the British Isles

Acoustic, clean plastic, acid-washed plastic tube, water, computer,

Carbon isotope values in otolith and muscle samples were highly correlated within and among coastal habitats. Moreover, d13C values of otolith AAs provided a purely

analysis and integrated stock mixture analysis based on otolith elemental composition data Stable isotopes of d18O and d13C in sagittal otolith carbonates were used to determine the stock structure of the polynemid Eleutheronema tetradactylum (Shaw) across tropical northern Australia, where this species is an important component of inshore commercial and recreational fisheries

methanol, Milli-Q water, ultrasonic bath, microscope

Observation, laboratory tested, sampel the flathead grey mullet (Mugil cephalus)

dietary record that avoided dilution from dissolved inorganic carbon. Otolith AAs served as a robust tracer of d13C values at the base of the food web, making compound-specific SIA a powerful tool for dietary reconstructions and tracking the movement of fishes across isoscapes.

Western Australia across northern Australian waters, throughout the Gulf of Carpentaria to locations on the Queensland east coast of Australia , covering a coastline length of _14 000 km. Locations sampled were Eighty Mile Beach [Western Australia ,

Monofilament gillnet, dryer envelope, nylon brush, ultrapure water, hydrogen peroxide, ultrasonic bath,centripuge, oxygen, carbon computer

The Sr/Ca and Ba/Ca ratios of a subsample of 14 otoliths showed that half of the fish killed were Acoustic estuarine residents, and the rest survey, originated from marine and Laboratory freshwater habitats. The multiple tested. causes involved in this fish kill implied that Sampel herring additional unknown factors (C. harengus) influenced the mullet populations on a large scale and induced them to move quickly and gather at this estuary

Observation, Laboratory

Results suggest that most west coast herring belong to interconnected populations subject to mixing and that populations are not discrete, so the current practice of assessments based on

10

Accurate knowledge of fish age and growth is crucial for species conservation and management of exploited marine stocks. In exploited species, age estimation based on otolith reading is routinely used for building growth curves that are used to implement fishery management models..

Roebuck Bay , Blue Mud Bay [Northern Territory Gulf of Carpentaria , Walker River , Roper River , Arthurs Creek [Queensland , Archer River , Love River , Cleveland Bay east coast , Keppel Bay and Port Alma

tested.

individual spawning components will probably not provide sufficiently Sampel the robust information polynemid for management advice. The Eleutheronema complexity of herring populations tetradactylum needs to be considered for both (Shaw) fisheries and coastal-zone management.

in the Gulf of Lions (France, NW Mediterranean Sea

Ruler, trawl, fishing line, beach seine, weight, freezer, microscope, stereomicroscope, computer, camera

The significant differences in the isotopic signatures of E. tetradactylum demonstrated that there is unlikely to be substantial movement of fish between these locations. The spatial separation of these populations indicates a complex fine spatial scale stock structure across northern Australia, with at least 11 stocks or management units present. The population subdivision of E. tetradactylum was evident along expansive stretches of open beach systems and within coastal embayments with no physical barriers such as headlands.

Observation, survey, laboratory tested. Sampel : gilthead seabream (Sparus aurata L.)

The results provided evidence that growth parameters inferred with VBGF must be used with high caution. Hence, VBGF turned to be among the less accurate for growth prediction irrespective of the dataset and its fit to the whole population, the juvenile or the adult datasets provided different growth parameters.