TYPHOID FEVER
Mode of Transmission - ingestion of contaminated food or through contaminated hands Infections a.) Gastroenteritis b.)Bacteremia and Extraintestinal infections c.)Enteric Fever (Typhoid Fever)
PRINCIPLE
Human serum containing Anti-O and Anti-H antibodies , visibly agglutinate a suspension of Salmonella O and H antigens
Anti-O and Anti-H antibodies were measured using bacterial suspension of killed Salmonella typhi and Salmonella paratyphi
MATERIALS
Reagents -Serum -Salmonella O antigens (Groups A, B, C, D) -Salmonella H antigens (Groups a, b, c d) Glassware and Apparatus -Serological pipetes, 0.1 mL -porcelain spot plate -wooden applicator sticks
VOLUME OF SAMPLE
Patients Serum
0.08 mL 0.04 mL 0.02 mL 0.01 mL 0.005 mL
Dilution
1:20 1:40 1:80 1:160 1:320
PROCEDURE
Dispense the required Px serum on each of the well (Use 0.1mL serological pipette )
Tilt the spot plate back and forth and read within 2 minutes
ANTIGEN
Somatic ( O Ag)
ANTIBODY
Anti-O
Anti-H
Anti-K
MANNER OF REPORTING
100% of organism agglutinates = ++++ (+4)
INTERPRETATION
titer to O Ag = ACTIVE INFECTION
titer to Vi Ag = CARRIER
THANK YOU!!!
Roasa, Priscilla Grace Rubico, Faye Marjorie C. Salvador, Franz Raniel Sanchez, Charisse Santos, Samuel 3DMT