Chapter 6
6.2 Chromosomes
Chromosome Structure
Sister chromatid
One of two attached members of a duplicated eukaryotic chromosome
Centromere
Constricted region in a eukaryotic chromosome where sister chromatids are attached
Chromosome Structure
Proteins organize DNA structurally
Allow chromosomes to pack tightly
Histone
Type of protein that structurally organizes eukaryotic chromosomes
Nucleosome
A length of DNA wound around a spool of histone proteins
Chromosome Structure
Chromosome Number
A eukaryotic cells DNA is divided into a characteristic number of chromosomes Chromosome number
Sum of all chromosomes in a cell of a given type A human body cell has 23 pairs of chromosomes
Diploid
Cells having two of each type of chromosome characteristic of the species (2n)
Karyotype
Types of Chromosomes
There are two types of eukaryotic chromosomes: autosomes and sex chromosomes Autosomes
Paired chromosomes with the same length, shape, centromere location, and genes Any chromosome other than a sex chromosome
Sex chromosomes
Members of a pair of chromosomes that differ between males and females
XX
XY
eggs X X
sperm X Y
X X X XX XX
Y XY XY
Karyotype
Key Players
Erwin Chargaff, Rosalind Franklin, Maurice Wilkins, James Watson, and Francis Crick
Two double-helix strands are held together by hydrogen bonds between nucleotide bases Chargaffs rules
Bases of the two DNA strands in a double helix pair in a consistent way: A = T and C = G Proportions of A and G vary among species
DNA sequence
The order of nucleotide bases in a strand of DNA
1) The two strands of a DNA molecule are complementary: their nucleotides match up according to base-pairing rules (G to C, T to A).
2) As replication starts, the two strands of DNA unwind at many sites along the length of the molecule.
3) Each parent strand serves as a template for assembly of a new DNA strand from nucleotides, according to base-pairing rules.
4) DNA ligase seals any gaps that remain between bases of the new DNA, so a continuous strand forms. The base sequence of each half-old, half-new DNA molecule is identical to that of the parent.
Mutations
Reproductive cloning technologies produce an exact genetic copy of an individual (clone) Reproductive cloning
Technology that produces genetically identical individuals
Therapeutic cloning
Using SCNT to produce human embryos for research
Clones
Clone produced by somatic cell nuclear transfer