Who Sell Cells?

Team 4

Content
Which cells or cell lines would you use to solve the Problem statement? Describe the cells you picked. Pick any two cell banks in Singapore. Find out how to obtain cells from them

Pricing for the cells/media/special supplements. Compare at least two sources.

What are the different types of cells that can be cultured in a cell culture facility. Talk about at least two different cell types and their culturing methods. What cells can be banked? Talk about at least two unique sources of cells.

Cell: MELANOCYTE
Storage: below -130 degree celsius

Source: epidermis (basal layer)

Growth properties: adherent

Morphology: stellar, multipolar or dendritic appearance

Population doubling capacity: 10 in complete growth medium

Dermal cell basal medium used

Biosafety level 1

Cell line: CRL 1619

Storage below -130 Source: skin Transfection host Biosafety level 1 Dulbecco's Modified Eagle's Medium

Incubation is at 37 degree celcius

Subcultivation ratio: 1:3 to 1:8

Renewed at every 2 to 3 days

Singapore Stem Cell Bank (SSCB)

Advance stem cell research and the development of stem cell based technologies Distributor of high quality human stem cell lines that are maintained and characterized Used for education/ research Procedures for Cell Line Ordering Step 1: Please complete the Simple Letter Agreement (SLA) Step 2: Print and sign 2 copies of the SLA Step 3: Scan the signed SLA and courier original signed copies to the SSCB. Step 4: Email and attached the signed SLA via Email. Step 5: Complete Order Form.

Cord blood bank: Cordlife Group

Cord blood is blood that remains in the umbilical cord and placenta after the umbilical cord is cut Immediately after the delivery of your baby, the cord is clamped and the baby will be separated from the cord. The doctor then collects the umbilical cord blood by inserting a needle into the cord vein, and draining the blood into a blood bag. Used for treatment of blood cancer, replace and regenerate damaged/ diseased bone marrow

Pricing for cells/media/special supplements

CRL 1619 (Human Skin Malignant Melanoma)
$431

Dulbeccos Modified Eagles Medium

$20

CRL 1439 (Brown Rat liver)

$431

F-12K Medium (500ml)

$18

Fetal Bovine Serum (500ml)

$309

Pricing for cells/media/special supplements

CRL-4027
Human Esophagus
Epithelium Baretts esophagus disease $8500!!!

Cell types being cultured

PCS 100 020 PCS 500 010

PCS 100 020

o Primary Coronary Artery Endothelial Cells

o Cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells. o Biosafety level 1
o Adherent o Angiogenesis, arteriolosclerosis, hypertension, oncology, drug screening, inflammation, vasodilator function.

PCS 100 020

Endothelial Cell Growth Kit-BBE o Added to Vascular Cell Basal Medium o Complete cell culture environment o Final concentration:

Bovine brain extract (BBE): 0.2% rh EGF: 5 ng/ml L-glutamine: 10 mM Heparin sulfate: 0.75 Units/ml Hydrocortisone: 1 g/ml Ascorbic acid: 50 g/ml Fetal bovine serum: 2%

PCS 500 010

o Umbilical Cord-Derived Mesenchymal Stem Cells
o Spindle-shaped, fibroblastlike o Biosafety level 1

o Adherent
o Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines

PCS 500 010

Mesenchymal Stem Cell Growth Kit o Added to Mesenchymal Stem Cell Basal Medium

o The final concentration :

rh FGF basic: 0.5 ng/ml rh FGF acidic: 0.5 ng/ml rh EGF: 0.5 ng/ml Fetal Bovine Serum: 2% L-Alanyl-L-Glutamine: 2.4 mM

Cells that can be banked?

Fat tissue from liposuction Unique cell that can be stored:
(1) Endothelial cell

Bank to obtain: (2) STEM CELLS

Cord blood/cord tissue from umbilical cord

Wisdom teeth/ Milk teeth