Anda di halaman 1dari 44

MEHANIZMI STARENJA I DUGOVJENOSTI

Prof. dr. sc.

Ivica Rubelj

Laboratorij za molekularnu i staninu biologiju Zavod za molekularnu biologiju, IRB

Skeptici 2013

to je starenje?
Starenje je proces sveobuhvatnog, ireverzibilnog i progresivnog tjelesnog propadanja koje se dogaa tijekom vremena.
Genes, Aging, and Immortality. Charlotte A. Spencer

Starenje

Eduard Veith - Fountain of Youth

Alchemist searching for the Philosopher's Stone Joseph Wright of Derby

Na dugovjenost se moe utjecati !

Porast broja stogodinjaka u SAD

Jeanne Louise Calment: 21 February 1875 4 August 1997

Starenje se odvija na staninoj razini !

Starenje kontroliraju telomere!

Stanino starenje
L. Hayflick

HL

In vitro

mladi fibroblasti

stari fibroblasti

In vivo
35 god.

75 god.

Accumulation of senescent cells in tissues

TELOMERE ?

Telomere

Telomere stanino starenje ?

Teorija marginotomije

mlade

stare

Telomeraza

Telomeraza

C. Greider

spolne stanice matine stanice tumorske stanice jednostaninici

Telomeraza u normalnim fibroblastima

J. Shay HL

W. Wright

HL

Ekspresija telomeraze na razini organizma

Mice overexpressing telomerase (TERT) age more slowly than normal mice. (Blasco laboratory)

Maria A. Blasco

Tert delays ageing. Survival curves and % mice living for more than three years. Neuromuscular coordination as % of mice passing the tightrope test.
Tomas-Loba et al. Cell 135, 609622, November 14, 2008

Or Not.

Reeksprimiranje telomereze na razini organizma


Ronald A. DePinho

TWO MICE INVOLVED IN AN EXPERIMENT ON AGE-RELATED DEGENERATION. MICE WHOSE TELOMERASE GENE WAS ACTIVATED SHOWED NOTABLE IMPROVEMENTS

Telomere kao indikatori dugovjenosti, bolesti, stresa...

Zato mjeriti telomere?

Adapted from: Tsuji, A., A. Ishiko, et al. (2002). "Estimating age of humans based on telomere shortening." Forensic Sci Int 126(3): 197-9.

Predikcija dugovjenosti?

Association of telomere length in blood DNA after age 60 with subsequent survival. Data are plotted as fitted and smoothed survival curves, according to the group prognosis method. Longer identifies individuals from the top half of the telomere length distribution and shorter those from the bottom half of the distribution. Years since draw means the interval ending with that year (eg, 71 people at risk at end of year 1 for longer group, both sexes).

The relationship between telomere length and survival in humans (after Bakaysa et al. 2007). Those with the longest telomere lengths have the highest survival prospects.

Dulje telomere bolje zdravlje

Telomere length (adjusted for age, gender, and group) according to the absence (gray bars) or presence (white bars) of various age-related diseases in the study subjects. Number of individuals analyzed in each group: hypertension (n = 125), without hypertension (n = 142), with the metabolic syndrome (n = 59), without the metabolic syndrome (n = 286), diabetes (n = 34), without diabetes (n = 276). The MMSE (Mini-Mental State Examination) was performed in centenarians only: MMSE score 25 (gray bars, n = 46) or >25 (white bars, n = 32). *P < 0.05.

ene ive due


Telomere Length in the Newborn

STRES

!
Telomere length and telomerase activity levels in extreme high- and low-stress groups. (A) Average telomere length and SE are shown. The highstress group had shorter telomeres even after controlling for age. (B) Average telomerase activity and SE are shown. The high-stress group had lower telomerase activity even after controlling for age.

Moe li se utjecati na nae telomere i starenje?

Longer lives: In tests, the anti-ageing pill increased the life expectancy of animals by 38 per cent

Starenje i prehrana

Resveratrol

Zdraviji metabolizam sporiji gubitak telomera

(Reprinted) JAMA, January 20, 2010Vol 303, No. 3

ANALITIKA TELOMERA
Laboratorij za molekularnu i staninu biologiju, IRB

Elizabeth Blackburn

Odluite ivjeti due i zdravije!

Anti-aging food

Sports & recreation

ZAHVALJUJEM NA POZORNOSTI