The physician/ doctor signature and seal who performed the specimen collection
HISTOPATHOLOGICAL DIAGNOSIS
Histochemical techniques:
PAS (neutral MPZ, glicogen, basal membranes, fungi);
Fontna/Grimelius (neurosecretor granules);
Gordon-Sweets, Gomori (reticuline fibres; basal membranes);
Perls (hemosiderin-trivalent iron);
Fontana (melanin);
von Kossa (calcium);
Sudan Black, Sudan 3, Scharlach (neutral lipids);
Congo Red (amyloid);
Alcian Blue (acid mucine).
HISTOPATHOLOGICAL DIAGNOSIS
The physician/ doctor signature and seal who performed the sample collection
CYTODIAGNOSIS
Time of execution of the smears
1. Displaying
• Directly: natural secretions, pus, samples obtained by aspiration
puncture from solid organs, printing;
• After Centrifuge 5-10 minutes, then displaying of the sediment:
Liquids from serosal fluids, cystic fluids, cysts, urine and lavage.
2.Fixation
• Agitation in the air a few seconds and drying (for staining with May-
Grunwald-Giemsa);
• Alcohol 96% (for staining of Papanicolau coloration-Pap);
3.Staining
• MGG-very simple
• Pap for cervicovaginal and bronchial cytology,
• Special for recording of lipids, glycogen, mucine
• Imunocitochimy
CYTODIAGNOSIS
2. Histoenzimology
- detecting enzymes;
3. Electron microscopy for differenciating between:
- Carcinoma-melanoma-sarcoma;
- Adenocarcinoma-mesothelioma;
- Thymoma-lymphoma-seminoma;
- Small cell tumors and fusiform cell tumors;
- Endocrine and non-endocrine tumors;
- Glomerular Nephropaties with immune mechanism;
4. Techniques of molecular biology:
- PCR (polymerase chain reaction);
- Cytometry in flux for DNA analysis (ploidy);
- In situ hybridization to identify DNA fragments;
- Analysis of gene rearrangement.