DIAGNOSTIC FUNDAMENTALS OF CLINICAL MICROBIOLOGY

RIGHT THERAPY

RIGHT DIAGNOSIS OF INFECTIOUS DISEASE

CONTROL

CLINIC

RADIOLOGY

RIGHT DIAGNOSIS OF INFECTIOUS DISEASE

LABORATORY DIAGNOSIS

P.A.

MICROBIOLOGY

P.K.

RIGHT SPECIMEN

METHOD Specific Sensitive

RIGHT MICROBIOLOGY DIAGNOSIS

EQUIPMENT /REAGENTS Tested

HUMAN RESOURCE Trained Highly dedicated i

SPECIMEN
GOOD

Right material - Right collection time - Right collection location - Right collection procedure - Right storage and transportation - Right quantity

SPECIMEN COLLECTION
1. PROCEDURE

Sterile : collection location, collection tool, container 2. TIME Before antibiotic therapy In certain stadium of disease where microbial agents of disease could be found. tifoid : Salmonella isolation from blood firs mg of disease furunkel: mature, or at incision In the mornings, collected at 24 h 3. LOCATION Where causative agent would probably be found : most bad, most deep paling dalam 4. AMOUNT : according to necessity.

SPECIMEN STORAGE

Container: sterile dan sealable (screw capped bottle). Storage time: must be immediately processed, may not exceed 24 hours. Temperature : depends on suspected bacteria : 4oC, room temperature. If necessary: transport medium

SPECIMEN TRANSPORTATION

Must be in suitable environment for the suspected bacteria. - transport medium - Room temperature /thermos bottle with ice inside Container may not be carried with bare hands. Long distance : CONTAINS MATERIAL THAT CAUSE DISEASE.

IDENTIFIKASI BAKTERIOLOGIS
SPECIMEN
BACTERIA MACROMOLECULE

Direct preparation

Bottle/ transport med.

Ag

Ab

DNA

Microskopic

Isolation

A. Pemeriksaan Mikroskopis
SPECIMEN
DIRECT PREPARATION
WET MOUNT

STAINING

GRAM

ACID FAST

FLUORESCENCE

Preparat langsung
1. Wet Mount - morphology - movement 2. Stained preparation a. Gram: morphology, Gram characteristic b. Acid fast: morphology, acid fast characteristic c. Fluorescence: me sensitivity

BIAKAN BAKTERI
SPECIMEN
BOTTLE/ TRANSPORT MED.

ENRICHMENT MEDIUM
SELECTIVE/DIFFERENTIAL MED

BIAKAN MURNI
Identification GRAM TES BK AG DNA Tes R

Medium
1. 2. 3. 4. 5. 6. 7. 8. Transport medium = carrier medium Basic medium Enriched medium Enrichment medium Selective medium Differential medium Special medium Anaerobic medium

Medium : artificial environment used to maintain life and to culture bacteria outside the host. Colony : mass of growing bacteria on solid medium Isolated colony: separated colony, decendant of a single bacterial cell.

Inoculating on solid media