Chromium: 1st dose – lose 45% of RDA; 9th dose – lose 15% of
RDA; p=0.0005; so, several days of DMSA results in several days
loss of chromium
Loss of essential minerals (cont.)
Molybdenum – decreased excretion by 6% on 1st day, 30% on 9th dose.
Probably not a concern.
Sodium: 1st dose – increased excretion of 30%, normal by 9th dose; not a
concern
Copper and Zinc: Increased excretion, but less than 1% of RDA
Vanadium – small loss (about 1/3 of daily intake)
Little change: calcium, phosphorus, magnesium, sulfur, selenium
500
250
Glutathione
Change in
0
-250 0 300 600 900
-500
-750
Initial Glutathione
49 continued to Phase 2
Phase 2 : Randomized, double-blind,
placebo-controlled
White Blood Cell -7% n.s. (initially 1 high, 0 low; then 1 high, 1
low)
Lymphocytes 0%
Potassium +0%
ALT (liver enzyme) +3% - initially 1 high, then all ok
AST (liver enzyme) -6% - all normal
BUN/creatinine (kidney) +8% - initially 10 high, then 9 high, 1 low
Statistically Significant changes:
Triglycerides: +47%, p=0.06 (trend) - initially zero high, then 2 high
Other changes (not significant):
Eosinophils: -19%, p=0.08 3 high, then 2 high
Basophils +50%, n.s. - all normal
Changes in blood chemistry after 1
round DMSA, 7 rounds placebo
Platelets: -13%, p=0.01 initially 1 high, then all normal;
measured 3-4 months after treatment, so effects are long-
lasting
7 point scale
3=much better
2=better
1=slightly better
0=same
-1=slightly worse
-2=worse
-3=much worse
Parent Global Impressions
Parent Impressions- Overall
ADOS exam
• Autism Diagnostic Observation Schedule
• Based on 1-hour standardized interaction
with child by ADOS-certified professional
• One of the “gold standards” for evaluating
severity of autism
ADOS results
7 rounds 1 round
Communication -9% -11%
Social Interaction -10% -2%
Play/Creativity -5% 0%
SBRI (stereotyped behavior and restricted
interests) -9% -2%
ATEC 96% 2% 2%
SAS 61% 33% 6%
ADOS (Comm. + 68% 15% 17%
Social)
PDD-BI 75% 2% 22%
(modified Autism
Composite)
Parent Global 86% 8% 6%
Impression
Average of all 5 77% 12% 11%
assessments
Regression Analysis – Severity of Autism
Compare severity of autism with glutathione and metal tests
adjusted R2 Most significant metals
• ATEC: 0.22 p=0.003 Pb-9, Sb-b
• SAS: 0.36 p=0.002 Pb-b
• PDD-BI: 0.25 p= 0.004 Sb-9, W-b, Sn-9
• ADOS: 0.49 p=0.0003 Hg-b, Al-b, Hg-9