BANANA TISSUE CULTURE

INTRODUCTION
Plant Tissue Culture is culturing of any part of the plant in a specially defined growth media under aseptic laboratory condition in Petri dishes, test tubes or in any other suitable glass containers. Banana plants produced from Tissue Culture are free from diseases at the time of supply and they give high yields. India is endowed with tropical climate with abundant solar energy throughout the year, which favours growing crops round the year.

There is a vast scope to increase irrigation potential by river projects and minor irrigation projects. In additional to the above, India is blessed with more labourer availability. Since agriculture is the primary sector. Total geographical area : 328.848 million. Total reporting area : 304.300 million. Area under cultivation : 143.000 million. Total cropped area : 179.750 million . Area sown more than once : 36.750 million. Area not available for cultivation : 161.300million. Area under forest : 66.400 million .

BREIF HISTORY OF TISSUE CULTURE IN BANANA

Realizing the importance of shoot meristem culture in getting rid of the plant disease causing pathogens and its subsequent mass propagation. Berg and Bustamante (1974) attempted technique. However, this procedure is not completed effectively in the absence of suitable diagnosis. Soon after reports of Banana plantlets produced by invitro shoot tip culture came from Taiwan (Ma and Shill, 1974).

MICROPROPOGATION
Production

of large number of plant lets within a short time less labor costs Large scale and commercially viable technologies were developed

IMPORTING OF BANANAS INTERNATIONALLY

ADVANTAGES

Large number of uniform propagules can be generated in a relatively short period of time. Variability encountered in size and propagules density especially in clones suckering erratically can be minimized. It could allow for rapid bulking of novel clones when used in concert with breeding programms.

TISSUE CULTURE TECHNOLOGY

Successful micro-propagation strategies are as a result of interplay of plant material, the culture milieu and the micro-environment.

SHOOTING

ROOTING

PRIMARY HARDENING
Primary hardening will take atleast 4 weeks depending upon the climatic conditions. In final week these trays are gradually exposed to 50% shade by removing plastic sheets. These plantlets are sprayed with fungicides, bactericide, and water soluble fertilizers as per schedule.

TISSUE CULTURE TRANSFER AREA

Is a separate air conditioned Dust free room Equipped with the requisite number of laminar airflow A high level of cleanliness ensures An ethanol dip, an alcohol lamp/ Bunsen/ burner/incinerator/hot bead sterilizer etc.

PHOTOGRAPHS SHOWING DIFFERENT STAGES OF TISSUE CULTURE BANANA PLANT

BANANA INITIATION STEP1

BANANA MULTIPLICATION STEP2

BANANA ROOTING STEP3

BANANA PLANTS IN NETPOTS STEP 4

BANANA PLANTS READY FOR SALE

STEP5

PRESENTED BY
Ashwini Parab Manish Kalshetty Amol Jadhav Tejas kamble Ajay pawar Kiran Dolas

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