Viruses
CYTOGENETICS
AY 2008-2009
Relevance of Bacterial & Viral
Genetics
• Microorganisms are the most important
component of environmental health
• Microorganisms cause diseases
• Microorganisms can help heal as well as
prevent disease
• Microorganisms have numerous
commercial/industrial applications
• Mitochondria and chloroplasts are
microorganisms
• Microorganisms serve as model system
• Microorganisms are extremely abundant
VIRUSES (Characteristics)
• Smaller than bacteria (typically, at least)
• Obligate intracellular parasites (some
bacteria are also)
• structurally simpler than cellular
organisms
• possess a relative dearth of metabolic
machinery
• Many possess unusual genomes
• Relative dearth of antivirals
• Go through an acellular stage
VIRUSES (Parts)
– The genomes of viruses are typically
much smaller than the genomes of
cellular organisms
– Virus genomes are also not always
composed dsDNA
(i) dsDNA
(ii) ssDNA
Figure 13.2a, b
Helical Viruses
Figure 13.4a, b
Complex Viruses
Figure 13.5a
VIRUSES (Host Range)
– Many viruses are limited to only a single
host species
(e.g. bacteriphage)
– Other viruses have broader host ranges,
being capable of successfully infecting
more than one host species
– Many viruses are additionally limited in the
cell types they are able to infect within a
host (i.e primary & secondary target)
– One determinant of the host range of a
virus is the "lock-and-key" fit between the
virus capsid or envelope proteins and virus
receptors, the latter of which typically
Viruses (Life Cycle)
The simplified virus life cycle consists of
(i) Adsorption to a host cell
cell
(iii) Transcription of virus genes
mRNAs
(v) Replication of the virus genome
into capsids
(vii) Progeny-virus release from the host
cell
Bacteria Bacterial Capsi DN
l cell chromoso
Capsi
Sheat
Tail fiber
1 Attachment: Tail
Base
Phage
attaches to Pin
Cell wall
Plasma
2 Penetration:
Phage
pnetrates host
cell and injects Sheath
Tail core
3 Merozoites
released into
bloodsteam from
liver may infect
new red blood
Figure 13.10.1
Tail
DNA
4 Maturation:
Viral components
are assembled
Capsi
into virions.
5 Release:
Host cell lyses
and new virions Tail fibers
are released.
Figure 13.10.2
Viruses (Life Cycle)
– A lytic life cycle requires the destruction
of the host cell before progeny release
may occur
– This host-cell destruction is called lysis
Viruses (Life Cycle)
• Lysogenic life cycle (prophage,
provirus, temperate virus)
– In a lysogenic life cycle virus progeny
are neither produced nor released
• Temperate virus = a virus capable of going
through a lysogenic cycle (e.g., phage lambda,
a.k.a., )
• Prophage = a bacteriophage whose genome has
integrated into its host's genome during
lysogenic growth
• Provirus = equivalent to prophage but more
generally applicable (e.g., to animal viruses)
Beneficial Uses of Viruses in
Biotechnology
• Gene therapy
• Vaccines and vaccine carrier / delivery
vehicles
• Antibacterial agents
• Insecticides
Bacteria
• Circular chromosome
• Extrachromosomal: plasmids
• Constitutive and repressible genes
• Transposons
Bacteria
• Sex:
– Transformation
– Transduction
– Conjugation