Lycopene and Cardiovascular

Disease
Presented by :
Anjali Tiwari
 Introduction
1. Lycopene and CVD disease ,
2. Plasma lycopene ,other caoteniods ,and
retinol and the risk of CVD in men .
3. Plasma lycopene ,other caoteniods ,and
retinol and the risk of CVD in women .
 What is lycopene ?
• Lycopene is hydrocarbon carotenoid
which has received attention for its
potential role in preventing prostate cancer
and CVD disease in humans .
• In 1989 Di Mascio et al showed that
lycopene has strongest oxygen quenching
capacity of several carotenoids , with
alpha –carotene ,beta –carotene ,and
lutein next in capacity.
 In study 1 it include
st

• Introduction
• Food sources and absorption of lycopene
• Lycopene and smoking
• Biological activity of lycopene
• Epidemiologic Evidence
 Study 2 nd
&3 rd
include
• Subject and methods
• Result
• Discussion
 In subjects and method of 2nd &3rd
study include
• Study population :-
The WHS is ongoing
randomized ,double –blind ,placebo controlled
2x2 factorial trial of the relationship between low
- dose aspirin vitamin E and primary prevention
of CVD & cancer.
39,876 female US health professionals aged >
45 y in 1992 ,were postmenopausal or not
intending to became pregnant , and had no
history of MI , stroke ,transient ischemic attack
or cancer were enrolled in study.
 Subject & method of 2nd & 3rd
study…….
Fasting baseline blood samples were
collected from 28,345 (71%) participants
and stored in liquid nitrogen until
analyzed .
 Subject & method of 2nd & 3rd
study…….
• Study population of 3rd study :
The PHS began as trial
in 1982 of 22,071 men testing aspirin and beta carotene
in the primary prevention f cancer and CVD ,it was
completed in 1995.
PHS 2 was initiated as randomized ,double blind ,
placebo- controlled ,2x2x2x2 factorial trial testing the
benefits and risk of vitamin E ,VIT C a multivitamin ,
and beta carotene in the primary prevention of cancer
,CVD and other chornic disease in male physicians aged
>50 y ,including 7641 original physicians from the PHS
plus 7000 new Physicians were recruited. fasting blood
samples were collected from PHS in 1996
 Subject & method of 2nd & 3rd
study…….
• Blood assays :-
Baseline blood samples from
cases & control were thawed and assayed for
total lycopene ,other carotenoids and retinol at
Our lady of Mercy Medical centre Bronx (NY).
All assays was quantified by conventional
method .Plasma lipid including total cholesterol
and HDL cholesterol , using commercially
available diagnostic kits (sigma –aldrich
chemical Co , St louis , Mo) .
 Subject & method of 2nd & 3rd
study…….
• Blood Assays…..
Plasma total cholesterol was assayed
because plasma lipoprotein are non –
specific carrier for all the caroteniods in
plasma &to date ,total cholesterol
appears to be best way to control for
confounding effects due to differences in
lipoprotein concentration between subjects
.
 Subject & method of 2nd & 3rd
study…….
• Baseline covariates :-
Women from WHS and Men
from PHS provided self –reported dad on age (in )
,weight & height (converted to body mass index ; in kg
/m2 ), smoking status (categorized as never , former , or
current ), alcohol use (categorized as rarely or never
,<1drink /d, or .1drink /d) ,frequency of exercise
(categorized as rarely or never , <3d/wk ,3-4d/wk or .5
d/wk), parental history of MI at <60 y of age (yes or no ),
and history of hypercholesterolemia (yes or no) .
 Subject & method of 2nd & 3rd
study…….
• Data analyses:
Men from PHS & women from
WHS were first compared to case control status
by using mean value or proportion of baseline
coronary disease risk factors and biochemical
makers .
Measurements of plasma lycopene
concentration were divided into quartiles based
on overall distribution of plasma lycopene
among the 499 for men in controls &483 for
women in controls.
Results of 2 study
nd