BIOTECHNOLOGY
One of the basic tools of modern biotechnology
is gene splicing.
This is the process of removing a functional
DNA fragment ( a gene) from one organism and combining it with the DNA of another organism to study how the gene works.
The desired result is to have the new organisms
carry out the expression of the gene that has been inserted.
Restriction Enzymes
The ability to cut and paste DNA
predictably is due to the use of restriction enzymes. They were first identified in and isolated from the bacteria that use them as a natural defense mechanism to cut up the invading DNA of bacteriophages viruses that infect bacteria. They are named for the
The negatively charged particles move toward the positive electrode while the the positive charge particles move toward the negative electrode.
How does electrophoresis work? The gel is made from agar DNA is a negative molecules Molecules sort based on Charge Size shape
What is agar?
Agar comes from sea weed. What is it used for?
Procedure
Remove comb and observe wells.
overflow into each end of the tray. Load gels. Connect the electrodes. Turn on power supply. Allow gels to run make sure you see bubbles coming from the electrodes.
PROCEDURE (CONTINUED)
It will take about 30 minutes for the gel to
run. Turn off power supply and remove electrodes. Pour off buffer into the designated container. Carefully remove gel from gel box and place in glad container and cover with stain. Store in appropriate location.
taking place. One electrode will have a lot of bubbles and the other will have a lesser amount. Why the difference? The formula for water is H2O and the splitting of the molecule will produce twice as many atoms of hydrogen.