Biotechnology
Chapter2 13
Traditional Applications
Traditional (historical)
applications of biotechnology
date back to over 10,000
years ago
• Use of yeast to produce beer
and wine in Egypt and Near East
• Selective breeding of plants
• Selective breeding of animals
Chapter4 13
Genetic Engineering
hromosome
Plasmid
Plasmid
1 µm replicates
in DNA
cytoplas fragment
m incorporat
ed into
chromoso
Chapter9 13
Transformation
90 °C 50 °C 72 °C
DNA
ragment
to be
amplified
2013
Chapter
Polymerase Chain Reaction
Larger
Direction fragments
of Migration move more
slowly;
smaller
fragments
move more
rapidly
2913
Chapter
DNA Fingerprinting
1 2 3 C S 4 5 6 7
R C
I E
SuspectsM NSuspects
E E
3113
Chapter
Findings
• Human genome contains ~25,000 genes
• New genes, including many disease-
associated genes have been discovered
• Has determined the nucleotide sequence
of all the DNA in our entire set of genes,
called the human genome
• The genes comprise 2% of all the DNA
4713
Chapter
The Human Genome Project
Applications
• Improved diagnosis, treatment
and cures of genetic disorders or
predispositions
• Comparison of our genome to
those of other species will clarify
the genetic differences that help
to make us human
4813
Chapter
DNA Probes
Viral
vector
with
herapeutic
gene Cell Culture Embryo with
defective gene
Treated Culture Genetically
corrected
Enucleated embryo clone
Genetically
egg cellcorrected
egg cell
netically corrected
enetically
cell from culture Healthy baby
Chapter 13
The end