OBJECTIVE
To development
immunological tests for
diagnosis, research and
transfer to the community
(2003-2007).
Colombia
Ecuador
Per
AMAZONAS
CAJAMARCA
PIURA
AMAZONAS
PIURA
PIURA
CAJAMARCA
CAJAMARCA
SAN MARTIN
AMAZONAS
AMAZONAS
AR CA
CAJAM
BARTONELLOSIS REPORTED BY
DEPARTMENT IN PERU
LORETO
SH
CA
AN
SAN MARTIN
LA LIBERTAD
ANCASH
ANCASH
HUANUCO
HUANUCO
HUANUCO
M
LI
ANCASH
LIMA
LIMA
LIMA
JUNIN
1995
CUSCO
CUSCO
AYACUCHO
1997
1999
2001
3258 cases
12 dead
12 departments
2005
2006
MINSA/OGE- 2006
Birtles R, Fry N, Ventosilla P, Cceres A, Snchez E, Vizcarra H, Raoult D.J. Clin Microbiol 2002;
40:3606-12 Identification of Bartonella bacilliformis genotypes and their relevance to epidemiological
Rolain JM, Novelli S, Ventosilla P, Maguina C, Guerra H, Raoult D. Immunofluorescence detection of B. bacilliformis flagella
in vitro and in vivo in human red blood cells as viewed by laser confocal microscopy. Ann N Y Acad Sci. 2003 Jun;990:581-4
M. W.
Band
Sensitivity
Specificity
M. W.
Band
Sensitivity
Specificity
65 kDa
76.3 %
(29/38)
87.5 %
(28/32)
65 kDa
86.1 %
(31/36)
87.5 %
(28/32)
12 kDa
42,1 %
(31/36)
100 %
(32/32)
12 kDa
88.8 %
(32/36)
100 %
(32/32)
26.3 %
(10/38)
100 %
(32/32)
13 kDa
41.6 %
(15/36)
100 %
(32/32)
13 kDa
Products: In technology
To have Western blot and Elisa with a
good sensibility and specificity.
We will have obtained a dot-blot by the
year 2007.
We have 940 sera, 110 plasma, 32 strains
of B. bacilliformis from different part of the
endemic area.
We have a media to produce antigens and
criopreserve.
Products
Products
4 Abstracts at International Congress were present:
Huarcaya E ; Maguia C ; Best I ; Solorzano N ; Menacho J Ventosilla P.
Immunological pattern of patients with acute and chronic phase of Bartonella
bacilliformis infection in a endemic area in Peru. 55th Annual Meeting the
American Society of Tropical Medicine and Hygiene (55
nov.12-16 : Atlanta,
Georgia. United States) In: The American Journal of Tropical Medicine and
Hygiene: nov. 2006: 75(5) supl.(Abstract 1066)
Ventosilla, Palmira; Maguia, Ciro; Best Ivan; Villar Sandra; Palma Sandra;
Henriquez Csar; Huacaya, Erick. Travel Award for presenting research
findings at a the 20th meeting of the American Society for Rickettsiology And the
5th International Conference on Bartonella as Emerging Pathogens in Pacific
Grove, Califronia, USA, September 2-7 2007.
S. Palma, M. Villar, M. Gonzles, C. Prause, C. Maguia, P. Ventosilla. Two
immunological test to diagnostic carrions disease (bartonellosis) in patients of
endemics areas in Peru. ECCMID-ICC meeting to be held in Munich from 31
March to 3 April 2007. Manuscript in preparation
R. M. Castillo, C. Terrones, D. Yabar, P. Ventosilla. Knowledge, Attitudes and
Practices regarding Bartonellosis of the residents of Ollantaytambo district, in
the Urubamba province, in the Sacred Valley of the Incas, Cusco-Peru, July
2004. In the 17th Latin American Symposium, 73rd AMCA Annual Meeting,
Orlando Florida, April 1-7, 2007.
Publication
12 publications:
Compromiso Inmunolgico en Pacientes con
Bartonelosis Humana. E. Huarcaya, I. Best, J. Merello,
C. Maguia, P. Ventosilla. Aceptado para la publicacin
por Fundacin Instituto Hiplito Unnue, en Diagnstico.
Report of an unusual case of persistent bacteremia by
bartonella bacilliformis in a splenectomized patient.
Henriquez C, Hinojosa JC, Ventosilla P, Infante B,
Merello J, Mallqui V, Verastegui M, Maguina C.
Am J Trop Med Hyg. 2004 Jul;71(1):53-55.
Chapter Bartonella, In Dermatology, C. Maguia, H.
Guerra, P. Ventosilla. London, it send to publish.
2 Publication will have made by the year 2007, about the
Western blot, Elisa and Dot-blot.
Obstacles
The main obstacles that our
group found was that 2004
and 2005 there were few acute
patients.
We have not training as
planning.