COMPLETE BLOOD COUNT

PH 156
Group 7

Complete Blood Count

Also known as the CBC, Hemogram or CBC
with differential
A laboratory test which measures circulating
cells in the blood (RBC, WBC, etc)

Importance
Gives information about the kinds and
numbers of cells in the blood
Helps doctors in checking symptoms, such as
weakness, fatigue, or bruising
Helps in diagnosing conditions like anemia,
infection, and many others
Monitor treatment for blood disorders

Principles
It is a panel of tests which includes:
WBC count
WBC differential
RBC count
Hemoglobin
Hematocrit
RBC indices
Platelet count

Procedures
Obtain blood sample from patient
Sample is sent to laboratory for analysis

Uses a Hemocytometer
Kit containing instruments for counting blood cells

Hemocytometer
Also known as counting chamber
Thick slide with 2 platforms that have grids for
counting blood cells
Contains calibrations
Depth is 0.1mm

Methodology
Put the cover slip on top of the grid area in the
counting chamber

Dilute the sample:

1:20 for WBC count
1:200 for RBC count and platelets
Diluents: Acetic acid or distilled H2O for WBC count;
Wright, Hayels fluid or normal saline for RBC

Methodology
Load the sample into the loading area in the
chamber
Examine under microscope then count the
cells in the 4 large squares for WBC

Methodology
Calculate the WBC count using the formula:

Methodology
Count the cells in the center square for RBC

Calculate the RBC count using the formula:

Interpretation
Usually reported along with a reference range
of expected or desired values
Consider other factors when interpreting
results such as personal and family medical
history

Interpretation