10 30 rule
AIDS
A patient specific approach to the decision to transfuse
blood components
What Hgb / Hct level poses greater risk to the patient than
the threat of contracting a transfusion-transmitted disease
greater degrees of anemia could be well tolerated
chronically anemic renal failure
Jehovahs witness
morbidity and mortality rates did not increase
until Hgb level fell below 7 g/dl
Do2 = Cao2 x CO x 10
Cao2 = Sao2 x Hg x 1.34 + Pao2 x 0.0031
The heart
has a high extraction ratio
must rely upon redistribution blood flow to O2 supply
greatest risk !!!
Chronic anemia
CO may not change until Hgb decreases to 7 8 g/dl
synthesis of supranormal level of 2,3 DPG begin at Hgb 9 g/dl
right shifted
Inability to redistribute CO
Abnormal Hemoglobins
Patient
consent
Low risk
younger patient
High risk
Atherosclerotic
vasc. Dse
Perioperative
ischemia
pulmonary dse
rapid blood loss
Anticipated post-op
blood loss
10
12
Compatibility testing
1. ABO Rhesus blood type identification
2. Antibody screening of donor plasma
3. Donor-Recipient cross-match
Compatibility testing
ABO Rhesus blood type identification
According to the antigens present on the surface of the RBC
Which antibodies are present in the serum
serum constitutively contains antibodies to the antigens
that are lacking on the RBC
Compatibility testing
ABO Rhesus blood type identification
Rh (D) antigen
when D antigen is not present on the surface of RBC,
anti-D antibodies are not constitutively present in the serum
Rh-negative exposes to donor RBCs with D antigen (Rh-pos)
will usually produce anti-D antibody (60-70%)
latent period
the reaction evolve slowly and may not be clinicall
apparent on first exposure
subsequent exposure of Rh-neg to Rh(D)-pos
acute hemolytic reaction
Compatibility testing
Antibody screening
Seek the presence of recipient antibodies against RBC antigens
commercially supplied RBCs
+ recipient serum
+ donor serum
Compatibility testing
The cross-match
Donor RBCs mixed with recipient serum
simulating the actual anticipated transfusion
3 phases
1. Immediate phase
2. Incubation phase
3. Antiglobulin phase
Compatibility testing
The cross-match
The immediate phase
Compatibility testing
The cross-match
The incubation phase
Requires 30-45 minutes
incubated in albumin or low-ionic strength salt solution
Detects
antibodies primarily in Rh system
incomplete antibodies
antibodies that attach to a specific antigen but do not
cause agglutination in a saline suspension of RBCs
Compatibility testing
The cross-match
The antiglobulin phase ( indirect antiglobulin test )
Is cross-match necessary ?
ABO-Rh status alone
99.8 % compatible
99.94% compatible
99.95% compatible
Emergency transfusion
Choices
Type-specific partially cross-matched blood
Type-specific uncross-matched blood
O-negative (universal donor) PRBCs
Emergency transfusion
Even if the patients blood type become known and available
after 2 units of universal donor has been transfused
Citrate intoxication
Signs
hypotension
narrow pulse pressure
VEDP
CVP
ECG
prolonged QT interval
widened QRS complexes
flattened T waves
Microaggregate delivery
2nd 5th day platelet aggregation
after 10th day larger aggregates, fibrin, degenerated white cells
pulmonary insufficiency
ARDS
Febrile reaction
1% of all RBCs transfusions
Symptoms
temperature > 1o within 4 hours of transfusion
defervesces within 48 hours
Treatment
Acetaminophen