OLEH:
TIM PRAKTIKUM HEMATOLOGI JURUSAN ANALIS
KESEHATAN POLTEKKES DEPKES BANDUNG
- appendicitis
- uremia
- kehamilan
:
Dengan penambahan larutan Turk maka darah
akan menjadi encer dan sel lain selain lekosit akan
lisis.
Alat-alat :
- Haemocytometer lengkap (Bilik Hitung, Dek
glass, pipet Thoma untuk lekosit, pipet
Thoma untuk eritrosit )
- Satu set alat untuk pengambilan darah
- Mikroskop
- Darah
- Alkohol 70%
- Kapas
- Larutan Turk :
:
1. Pengenceran dengan pipet thoma lekosit
- Siapkan bilik hitung dengan deck glass
- Bersihkan jari pasien dengan alkohol 70%.
- Tunggu sampai kering, kemudian tusuk
dengan Vaccinostyle
- Darah yang keluar isap dengan pipet Thoma
lekosit sampai angka 1 (pengenceran 10
kali) atau sampai tanda 0,5 (pengenceran 20
kali).
.
bilik hitung.
Tunggu beberapa saat (3 menit).
Hitung dalam 4 kotak besar (64 kotak sedang
berukuran mm x mm).
=
P(pengenceran) x V(volume) x N (jumlah sel).
1. P = Pengenceran
= 10 kali atau 20 kali
2. Koreksi Volume = volume = p x l x t x jumlah kotak
= mm x mm x 1/10 mm x 64 kotak
= 64 / 160 = 1/2,5 mm3 = 2,5/mm3
3. N = Jumlah sel dalam 64 kotak.
Nilai Normal
LEUKOCYTE
ERYTHROCYTE
1. THOMAS PIPET
2. COUNTING CHAMBER
3. DILUTING FLUID
..
TURK
- HAYEM
- FORMAL CITRATE
4. HEMOLANCET
COTTON + 70% ETHANOL
COUNTING CHAMBER =
IMPROVED ..NEUBAUER
BURKER , ETC.
COLOUR BAND
&
NEWTONS RING
IMPROVED NEUBAUER
LEUKOCYTES COUNT :
4 CORNER SQUARES ( L ) = 1 X 1 X 0.1 mm3
ERYTHROCYTES COUNT :
1 X
1 X 0.1
5 SQUARES ( E )
mm3
80 SQUARES :
5
1 X
20
1
20
5
X
1
10
mm3
LEUKOCYTE COUNT
..
TURK
SOLUTION
COUNT BY MICROSCOPE
CONDENSOR : DOWN WARD
OBJECTIVE : 10 X
CALCULATION :
IMPROVED NEUBAUER
COUNTING CHAMBER
LARGE CORNER SQUARES
1 mm3 = N. CELLS
4
1X1X
10
4 mm3 = N. CELLS
101 mm3 = N X
X 420
10200
=NX
CELLS
4
NORMAL :
Dewasa
Bayi baru lahir
1-6 bulan
4-7 tahun
8-12 tahun
concentration
6.000.000
5.000.000
4.000.000
3.000.000
2.000.000
1.000.000
0
Erythrocytes Leukocytes Thrombocytes
Concentration / l 5.000.000
6.000
250.000
CBC.
Manual method
Cover slip
Hemocytometer
Thoma pipett
2.
W - WBC
R - RBC
PPlatelets
3.
4. R
R
R
5.
Automated method
Electrical Impedance
resistance
direct current
(approx. 100 V)
external electrode
vacuum
external
electrod
e
internal
electrode
internal electrode
vacuum
orifice
blood cells
orifice
sample
sample beaker
Automated method
Light Scattering
Cells are detected and counted as they pass through a focused beam of
light instead of through an electrical field.
Cells suspended in diluent pass through a conical chamber called a flow
cell, then the cells are irradiated with a beam of light from a light source
(i.e. laser beam) generating a scattered light.
Different cells have different light scattering intensities that could be
proportional to their cell size and intracellular density
Diluent
RBC
Isotonic solution
WBC
Recommended
lack of accuracy
and reliability
1%ammonium oxalate
Automated
Not done due
2% acetic acid
PLT
Manual
Recommended