membrane I
(Diffusion & Equilibrium Potential)
OBJECTIVES
1. Define diffusion potential of an ion and simply
conclude how to calculate it
2. Discuss the concept of charge separation.
3. Explain the methods of calculation of equilibrium
potential when the membrane is permeable to
several ions.
4. Define Donnan equilibrium and discuss its
consequences
5. Apply this knowledge to a practical instance.
BASICS FACTS
Molecular Gradients
outside
(in mM)
Na+
142
inside
(in mM)
14
140
Mg2+
1-2
0.5
Ca2+
1-2
10-4
K+
H+
HCO3Cl-
(pH 7.4)
28
110
(pH 7.2)
10
5-15
SO42-
PO3-
75
40
protein
Lipid Bilayer
CO2
ions
glucose
N2
H2O
urea
O2
halothane
Diffusion
(b)
Ion Channels
Characteristics:
1. Ungated
Determined by size, shape, distribution of charge, et
2.Gated voltage (e.g. voltage-dependent Na+ channels)
chemically (e.g. nicotinic ACh receptor channels.
in
Na+
out
Ion concentrations
Outside of
Cell
K+
Na+
Cl-
K
+
Na
Cl
Inside of
A-
Na+
Cl-
Outside of Cell
K
+
(K+) can pass
through to
equalize its
concentration
Na+
Cl-
Inside of Cell
Na+ and Clcannot pass
through
A-
- 55 to -100m
Result - inside
is negative
relative to
outside
+
+
+
+
+
+
- -
Na+
+
+
+
+
-+ +
+
Cl
- +
-
+ +
Hydration Shells
Basic Concepts
Forces that determine ionic movement
Volt;- A charge difference between 2 points
in space
1. Electrostatic forces
1. Opposite charges attract
2. Identical charges repel
2. Concentration forces
1. Diffusion movement of ions through semipermeable
membrane
2. Osmosis movement of water from region of high
concentration to low
ELECTRONEUTRAL
DIFFUSSION
LOW SALT
CONC;
HIGH SALT
CONC;
+
+
+
+
+
-
+
-
+
-
ELECTRONEUTRAL DIFFUSSION
HIGH SALT
CONC;
+
-
+
-
LOW SALT
CONC;
+
-
+
-
BARRIER REMOVED
Diffusion Potentials(DP)
is the potential difference generated across a membrane when a charged
solute (an ion) diffuses down its concentration gradient.
( caused by diffusion of ions.)
can be generated only if the membrane is permeable to that ion.
FEATURES;-1. if not permeable to the ion, no DP will be generated no matter
how large a conc; gradient is present.
2. magnitude/Unit =, measured in mV,
3. depends on the size of the concentration gradient, where the concentration
gradient is the driving force.
4. Sign of the DP depends on the charge of the diffusing ion.
5. DP are created by the movement of only a few ions, and they do not cause
changes in the concentration of ions in bulk solution.
At Electrochemical Equilibrium:
Equilibrium
4.Concentration gradient for
the ion is exactly balanced
by the electrical gradient
5.No net flux of the ion
6.No requirement for any
sort of energy-driven pump
to maintain the concentration
gradient
[K ]
RT
E
log
ZF
[K ]
EK = -90mV
ENa = +60mv
Z is the charge, 1 for Na+ and K+, 2 for Ca2+ and Mg2+, -1 for ClF is Faradays Constant = 9.648 x 104 Coulombs / mole
R is the Universal gas constant = 8.315 Joules / Kelvin * mole
T is the absolute temperature in Kelvin
CAPACITANCE
1. Cell membranes form an insulating barrier that acts
like a parallel plate capacitor (1 F /cm2)
2. Only a small number of ions must cross the membrane to
create a significant voltage difference
Electrical properties
The membrane potential
difference of -50
to +120mV
THE MEMBRANE
POTENTIAL
Extracellular
Fluid
K+
Na+
Potassium channel is
more open causing
potassium to be faster
M
E
M
B
R
A
N
E
Intracellular
Fluid
Sodium channel is
less open causing
sodium to be slower
MEMRANE POTENTIAL
(ABOUT 90 -120 mv)
2.
3.
Therefore,+ve ions will tend to gravitate towards -ve solution. Likewise, -ve ions will
tend to gravitate towards +ve solution.
4.
Then the difference between the inside voltage and outside voltage is determined
membrane potential.
1.
Membrane Potential:
Goldman Equation
P [ K ] P [ Na ] P [Cl ]
RT
V
log
F
P [ K ] P [ Na ] P [Cl ]
Na
cl
Na
cl
NOTE:
P = permeability
1.
2.
3.
P = permeability
At rest: PK: PNa: PCl = 1.0 : 0.4 : 0.45
Net potential movement for all ions
Known Vm:Can predict direction of movement of any ion ~
Vm
g
g
Na
E
Cl
g
g
Na
Na
E
Cl
Na
g
g
Cl
Na
E
Cl
Cl
Passive distribution
Donnan equilibrium
The ratio of positively charged permeable
ions equals the ratio of negatively charged
permeable ions
Start
Equilibrium
II
K+
Cl-
II
[K+] = [K+]
[Cl-] = [Cl-]
Mathematically expressed:
[ K ]I [Cl ]II
[ K ]II [Cl ]I
Another way of saying the number of positive
charges must equal the number of negative
charges on each side of the membrane
1.
2.
Start
AK+
Cl-
Equilibrium
II
II
Equilibrium
A- --[K+]----
[Cl-]
+ve = -ve
+
+
+
+
+
+
+
+
+
+
+
>
II
[K ]
+
< [Cl-]
+ve = -ve
space-charge neutrality
EXAMPLE
1.
50 K+
50 Pr -
50 K+
50 Cl100 Osmoles
Step 2
100 Osmoles
67 K+
17 Cl50 Pr -
33 K
33 Cl66 Osmoles
Final
Ions
Move
134 Osmoles
+
33 K
33 Cl33 ml
Total Volume
100 ml
67 K+
17 Cl50 Pr 67 ml
H2 O
moves
Human Potentials
1.
2.
3.
4.