CHAIN REACTION
(PCR)
DNA
Sources
Can amplify from any source
Bacteria
Viral
Plant
Animal
DNA Ligase
Primase
Helicase
Topoisomerase
Single strand binding protein
PCR
The DNA, DNA
polymerase, buffer,
nucleoside
triphosphates, and
primers are placed in
a thin-walled tube
and then these tubes
are placed in the
PCR thermal cycler
PCR Thermocycler
of DNA.
sample.
The basis of PCR is temperature changes and the effect that these
times
(note: 25 cycles usually takes about 2 hours and amplifies the
DNA fragment of interest 100,000 fold)
target DNA
Forward Primer
extension
VIDIO OF PCR
ADVANTAGES AND
APPLICATIONS OF
PCR
ADVANTAGES
Mainly speed and sensitivity mechanism for
of PCR:
Huntington's disease
cystic fibrosis
Human immunodeficiency virus
sequenced (a technique by
which the exact nucleotide sequence is determined) and the
number of trinucleotide repeats is then counted.
HIV
Defection of low abundance nucleic acid sequences.
HIV is a retrovirus that attacks the immune system.
Difficult to detect at early stage of infection using conventional
methods.
HIV tests rely on PCR with primers that will only amplify a section
Protein detection based tests are available as well but all US blood
is tested by PCR.
PCR offers a rapid and sensitive method for detecting viral , DNA
Paternity
Identifying
Bill clincton,former
president of USA and
secretary Monika
lewinsky case
THANK YOU
LEARNING RESOURSES
Lippincott`s Biochemistry,5th
edition,Editors:Richard A.Harvey,Pamela
C.Champe
Harper`s Biochemistry
You tube vidio
Internet