A Comparative Study of the Scabicidal

Activity of Prepared Ointments from

Makabuhay Stem (Tinospora rumphii L.,
Menispermaceae) and Akapulko Leaves
(Cassia alata L., Leguminosae) in Dogs
Infected with Sarcoptes scabiei Mite
Rona Jerise P. Angeles
Sharmaine Rizzel T. Angeles
Lovely Ann D. Ayapana
Len Mariz D. Castro
Ronaliz DJ. Ganancias

Dogs are the best friends

humans have. Shouldnt we
be too?

INTRODUCTION
Scabies is zoonotic.

However, the animal mite cannot reproduce

on a person and will die on its own.

INTRODUCTION (continued)
Sarcoptes scabiei infestation is a contagious
disease of dogs found worldwide.

INTRODUCTION (continued)
Life cycle

Adult
females
deposit
eggs as
they
burrow

Eggs
hatch
releasing
larvae

Larvae
migrate to
the skin
surface
and
change
into adult
form.

Mating
occurs on
skin
surface

The
characteristic itchy
rash of
scabies is
an allergic
response
to the
mite

INTRODUCTION (continued)
Makabuhay stem (Tinospora rumphii Boerl L., Menispermaceae)

vs.

Akapulko leaves (Cassia alata L., Leguminosae)

BACKGROUND OF THE STUDY

The study is done to
prove the folkloric use of
Makabuhay stem (Tinospora
rumphii L.,Menispermaceae)
as a scabicidal agent.

The researchers would

also like to pursue this study
to contribute to the progress
of new studies for this skin
disease.

OBJECTIVE OF THE STUDY

This study aims to prove the
scabicidal
activity
of
prepared
ointments from Makabuhay stem
(Tinospora
rumphii
Boerl
L.,
Menispermaceae)
that
will
be
compared to Akapulko leaves (Cassia
alata
L.,
Leguminosae).

STATEMENT OF THE PROBLEM

The study will seek the answers
to the following problems:
1. What are the physical and chemical
properties of the Makabuhay stem
(Tinospora rumphii Boerl L.,
Menispermaceae)?
2. What are the physical properties of the
prepared ointment from Makabuhay stem
(Tinospora rumphii Boerl L.,
Menispermaceae)?

STATEMENT OF THE PROBLEM

(continued)

3. Will the prepared ointment from

Makabuhay stem (Tinospora rumphii
Boerl L., Menispermaceae) cause
irritation in dogs
4. Does the prepared ointment from
Makabuhay stem (Tinospora rumphii
Boerl L., Menispermaceae) have
scabicidal activity in dogs infected with
Sarcoptes scabiei mite?

STATEMENT OF THE PROBLEM

(continued)
5. Is there a significant difference
between the scabicidal activity of the
prepared ointment from Makabuhay
stem (Tinospora rumphii Boerl L.,
Menispermaceae) compared to the
prepared ointment from Akapulko leaves
(Cassia alata L., Leguminosae)?

SIGNIFICANCE OF THE STUDY

Dogs and people infected with scabies
Pet lovers/owners
Makabuhay plant growers/producers
Veterinarian and other healthcare
professionals
The future researchers.

METHODOLOGY

Collection of
Makabuhay
Stems

Authentication of
Makabuhay
Stems

Preparation of
Ointment from
the Stems of
Makabuhay

Physico-chemical
property
determination of
Makabuhay Stem

Physical
Property
Determinatio
n

Organoleptic
Evaluation

Chemical
Property
Determinatio
n

Phytochemical
Screening &
Analysis

Alkaloids
Alkaloids
Flavonoids
Flavonoids
Glycosides
Glycosides
Plant Acids
Plant Acids
Carbohydrates
Carbohydrates

Saponins
Saponins
Tannins
Tannins
Fixed Oils
Fixed Oils

Formulation of Makabuhay stem

Ointment
Makabuhay Chopped stem.7.5g
White wax...................7.5g
Mineral oil.q.s
To make 50g

Positive
control:
Akapulko
leaves

Experimental
group:
Makabuhay
stem

Normal
control:
Mineral oil

Four
Subject
Groups

Normal
control:
White wax

Thank you!