Topik

I.

Bentuk

TugasMahasiswa

Bioteknologi
Ceramah

KONTRAKKULIAHBIOTEKNOLOGI

JumlahPertemuan

Agro

Ceramah

dandiskusi

Agro

Ceramah

dandiskusi

Agro(1)
HPT(2)

Ceramah
dandiskusi

1
2

Tim
HPT
Agro+HP
T
(Tim
Kelas)
Agro+HP
T
(Tim
Kelas)
Agro+HP
T
(Tim
Kelas)

Pertanianmeliputi
SejarahSingkat,
PengertianDasar
danRuangLingkup
TeoriDasar
Bioteknologi(Bahan
Genetik,Replikasi
DNA,Ekspresi
GenetikdanDNA
Rekombinan)
Teknik-TeknikDasar
bioteknologi(teknik
padatingkatprotein
danDNA)
EvaluasiI
Bioteknologi
Konvensionaldan
Modern
Bioteknologi
PemuliaanTanaman

dandiskusi

Diskusi
kelompok

Telaah
Jurnal

VII.

BioteknologiTanah

Diskusi
kelompok

Telaah
Jurnal

VIII

Bioteknologi
Perlindungan
Tanaman

Diskusi
kelompok

Telaah
Jurnal

II.

III.

IV.
V.

VI.

Dosen

Acara

Jadwal
(Mingguke)

PenanggungJawab

Isolasi/ekstraksi dan pengukuran Kandungan

Protein

Prof. Tri Agus Siswoyo

II

Pola Protein pada Tanaman ( Elektroforesis)

Prof. Tri Agus Siswoyo

III

Isolasi/ekstrasi DNA (Plasmid) dan pengukuran

Kandungan DNA/plasmid

Prof. Tri Agus Siswoyo

IV

Elektrophoresis dan Visualisasi DNA

Prof. Tri Agus Siswoyo

Amplifikasi DNA (PCR)

Prof. Tri Agus Siswoyo

VI

Elektrophoresis dan Visualisasi PCR Produk

Prof. Tri Agus Siswoyo

VII

Evaluasi

Prof. Tri Agus Siswoyo

INTRODUCTION TO PLANT
BIOTECHNOLOGY

Biotechnology ?

The application of biological organisms, systems or

processes to manufacturing and service industries
The integrated use of biochemistry, microbiology and
engineering sciences in order to achieve technological
(industrial) application capabilities of microorganism,
cultured tissue cells and part thereof
A technology using biological phenomena by copying
and manufacturing various kinds of useful substance
The application of scientific and engineering
principles to the processing of materials by
biological-agents to provide goods and services

Biotechnology ?

The science of the production processes based on the

action of microorganisms and their active components
and production processes involving the use of cells and
tissues from higher organisms. It was not included
medical technology, agriculture and traditional crop
breeding
The use of living organism and their component in
agriculture, food and other industrial processes
The use of microbial, animal and plant cells or enzymes to
synthesize, breakdown and transform materials
The integration of natural sciences and organisms, cells,
parts thereof and molecular analogues to product and
services

Stages of Biotechnology
Development

Ancient biotechnology
early history as related to food and shelter; Includes
domestication

Classical biotechnology
built on ancient biotechnology; Fermentation promoted
food production, and medicine

Modern biotechnology
manipulates genetic information in organism; Genetic
engineering

Stages of Biotechnology
Development

Traditional/old biotechnology
The conventional techniques that have been used to
produce beer, wine, cheese, many other food
New/modern biotechnology
All methods of genetic modification by recombinant DNA
and cell fusion techniques, together with the modern
development of traditional biotechnological process

Areas of Biotechnology

Organismic biotechnology
uses intact organisms; Does not alter
genetic material

Molecular biotechnology
alters genetic makeup to achieve
specific goals
Transgenic organism- an organism with
artificially altered genetic material

Biotechnology:
A collection of technologies

The Applications of
Biotechnology

Medical Biotechnology

Agricultural Biotechnology

Diagnostics
Therapeutics
Vaccines
Plant agriculture
Animal agriculture
Food processing

Environmental Biotechnology

Cleaning through bioremediation

Preventing environmental problems
Monitoring the environment

Plant agriculture

Crop production and protection

Genetically engineered (transgenic) crops

Using biological methods to protect crops
Exploiting cooperative relationships in nature

Nutritional value of crops

Improving food quality and safety

Healthier cooking oils by decreasing the concentration of
saturated fatty acids in vegetable oils
Functional foods
Foods containing significant levels of biologically active
components that impart health benefits

Plant Biotechnology

TRADITIONAL PLANT
BIOTECHNOLOGY

breeding
tissue culture
inter-specific hybridisation
mapping phenotypic/biochemical markers

PLANT BIOTECHNOLOGY
a process to produce a genetically modified plant
by removing genetic information from an
organism, manipulating it in the laboratory and
then transferring it into a plant to change certain
of its characteristics .
Now use a molecular approach to manipulation:
molecular markers & mapping
gene cloning
plant transformation

pathogen-derived genes
Exogenous genes
(non-plant genes)

bacterial genes
any other organism

Pathogen resistance
Applications:

Herbicide resistance

transgenic

bioreactors
Delivery systems

Endogenous genes
(Plant genes)

Enzymes in biochemical pathway

Natural resistance genes
Mapping

Gene discovery
(functional genomics)

ESTs, libraries
Silencing, expression
Mutants, arrays

Applications:
markers
transgenic

Marker assisted breeding

Plant improvement