Rapid Dengue Duo

Test (IgG+IgM)
A Rapid qualitative Immunochromatographic test for
the simultaneous detection of IgG and IgM Antibodies
to Dengue Virus in Human Serum, Whole Blood or
Plasma
DENGUE VIRUS
Most common arthropod-borne disease worldwide with an
increasing incidence in the tropical regions

Can be life-threatening

Caused by 4 closely related serotypes (serotypes 1-4)

Genus Flavivirus, Family Flaviviridae

Transmitted by Aedes aegypti and Aedes albopictus

mosquitoes

 “Breakbone" or “Dandy fever." 

DENGUE VIRUS

Infection confers life long immunity

But cross protection between serotypes

is of short duration.

Reinfection with different serotype

after primary attack is more dangerous
causes Dengue Hemorrhagic Fever.
 Aedes aegypti Aedes albopictus
 Rural dengue
Urban dengue
 “Tiger mosquito”
Breeds in clear water stored indoor and
 outdoor
Breeds incontainers such
clear water as bamboo
stored indoor stumps,
and
empty
outdoorcoconut shells,
containers such and
as tree holes.vases,
old tires,
jars and bottles
SIGNS AND SYMPTOMS
 Malaise
 Severe headache
 High fever and chills
 Severe back ache, joint
pains, muscular pain
SIGNS AND SYMPTOMS

Temperature may persist for 3 -5

days.

On some occasions once again

raises in about 5 – 8 days ( Saddle
back fever )

Nausea, vomiting and diarrhea

Red eyes, pain in the eyes

SIGNS AND SYMPTOMS

Biphasic pattern- fever lasts

about two to seven days,
with a smaller peak of fever
at the trailing end of the
disease.

Clinically, the platelet count

will drop until the patient's
temperature is normal.
DENGUE HEMORRHAGIC FEVER

Severe,
potentially
deadly infection
Common in
spread by certain
species of
children.
mosquitoes
(Aedes aegypti).

In other ( Adults )
the presence of Initially
antibodies due to presents like
previous infection classical Dengue
with different infection
serotype
DENGUE HEMORRHAGIC FEVER

Symptoms

similar to those of dengue fever, but after several days the

patient becomes irritable, restless, and sweaty. These
symptoms are followed by a shock -like state.
PATHOGENESIS

Presence of existing Dengue antibody, associated with fresh viral

infection with new serotype complexes and forms within few
days of the second dengue infection.

Non neutralizing enhancing antibodies promote infection of

higher number of Mononuclear cells.
DIAGNOSIS
Primary infection- patients diagnosed with dengue infection in non- endemic areas.

Secondary infection- patients diagnosed with dengue infection in endemic areas.

Hemorrhagic tendency

Thrombocytopenia 

Evidence of plasma leakage

Encephalitic occurrences
TREATMENT
No Anti viral therapy available

No Vaccine available

Symptomatic management in majority of cases

Dengue Hemorrhagic fever to be treated with suitable fluid replacement

Increased oral fluid intake is recommended to prevent dehydration.

Supplementation with intravenous fluid may be necessary.

platelet transfusion
RAPID DENGUE DUO TEST
PRINCIPLE
SUBSTANCE SAMPLE CONTROL
TEST AREA
DETECTED PORT AREA

Human IgG Colored- Specific Anti-rabbit

and IgM Dengue Human IgG Ig antiserum
antibodies specific and IgM
against recombinan binding
Dengue t antigen- proteins
Virus colloidal
gold
conjugate
RAPID DENGUE DUO TEST
 Reagents and Materials Supplied

1. FirstSign™- Dengue Duo(WB)

Test Card: 25 Individually pouched
Card tests with a dessicant pouch
and a Sample loop
2. Running Reagent – 1 bottle
3. Product Insert
RAPID DENGUE DUO TEST

Storage and
Stability
The sealed
pouches in
the test kit
and the kit
components
4-30˚C
RAPID DENGUE DUO TEST
 Testing Procedure

Kit Room
Components temperature

 Once the pouch is opened and the card is

retrieved, it must be used immediately.
Label the card
with patient’s
identity
RAPID DENGUE DUO TEST
5µl of
serum/plasma or Sample
whole blood port A

micropipette

Dip the loop Important!

5µl sample loop
into the
sample loop does
kit
not retrieve
clots or
Blot into the
sample port
debris from
“A” the sample
RAPID DENGUE DUO TEST

4 drops of Reagent
the Running port “B”
Reagent

Read the
results after
15 minutes
INTERPRETATION OF RESULTS

Negative result:
The presence of only the single
red/purple colored band in the control
window “C” indicates the absence of
specific antibodies against Dengue virus
or the amount of antibodies is below the
detection limit of the test.
INTERPRETATION OF RESULTS

Positive result: IgG and IgM

Appearance of two red/purple colored bands
in the test window at region ‘M’ and region
‘G’
Presence of Dengue virus specific IgM and IgG
antibodies
Acute secondary infection
INTERPRETATION OF RESULTS

Positive result: IgM

Appearance of red/purple colored band in
the test window at region ‘M’
Presence of Dengue virus specific IgM
antibodies
Acute primary infection
INTERPRETATION OF RESULTS

Positive Result: IgG

Appearance of red/purple colored band in
the test window at region ‘G’
Presence of Dengue virus specific IgG
antibodies
Acute secondary infection
INTERPRETATION OF RESULTS

Invalid result
If after 15 minutes no band is visible
either in the test or control window
The test should be repeated with a new
test card
LIMITATION

Limited to the detection of antibodies against Dengue viruses in

serum/ plasma.

Test should be repeated with fresh collected samples if negative

results were achieved while clinical symptoms are indicative of
Dengue virus.
LIMITATION

A definitive clinical diagnosis should not be based on the results

of a single test but should rather be made by a clinician after all
clinical findings have been evaluated.

Repeatable reactive specimens should be conformed by another

qualified test.