It is Greek for
of first
importance.
What does
protein
mean?
PROTEINS
Carbon, Hydrogen, Oxygen, Nitrogen
and sometimes Sulphur
Polymers made up of Amino acids
(monomer)
20 commonly occurring amino acids
(carbonyl)
R group
Glycine
Alanine
CH3
Cysteine
CH2SH
How do amino
acids join
together? And
what do they
form?
Hint: Think back to
carbohydrates
Atomic R
Group
Lysine (Lys)
Valine
(Val)
Phenylalanine
(Phe)
Alanine (Ala)
How is a protein
Constructed?
Polypeptide chain
Hydrolysis
In the same way as carbohydrates, the
peptide bond between two amino acids
can be broken in hydrolysis by the
addition of water. This produces two
separate amino acids again.
Structure of Protein
Primary
Secondary
Tertiary
Quaternary
Primary Structure
Importance of primary
structure
Determines ultimate shape and
function of the protein
A change in any one amino acid in
the primary sequence, could lead
to change in shape or function of
the protein
Therefore, proteins shape is VERY
SPECIFIC to its function
Secondary Structure
Tertiary structure
Tertiary structure of
proteins
The polypeptide chains often fold to
give unique complex 3D structure of
protein
This is maintained by:
Weak hydrogen bonds (easily
broken)
Stronger ionic bonds formed
between carboxyl and amino
groups (can be broken by changes
in pH)
Even stronger disulfide bonds (not
easily broken). These covalent
bonds form between R groups that
Quaternary structure of
proteins
Some proteins consist
of more than one
polypeptide chain or
polypeptide chains and
non-protein (prosthetic)
groups (eg:
haemoglobin contains
iron), which gives a
fourth level of structure
This shape is created
when the different
chains bind together to
form whole protein
molecule
http://www.johnkyrk.com/aminoacid.html
https://mywebspace.wisc.edu/jonovic/web/proteins.
Fibrous protein
Insoluble because Rgroups are non-polar
Secondary structure
of long polypeptide
chains forming
fibres (alpha helix)
or sheets (beta
pleated sheet).
Fibres give strength,
sheets flexibility.
Collagen
Made up from 3
polypeptide
chains (alpha
helix) twisted
together giving a
strong structure.
Globular Proteins
Highly folded and coiled with more than 1
polypeptide chain (complex tertiary
structure)
Each chain is called a subunit
Hydrogen, ionic and sulfide bonds hold its
shape
Soluble with a specific tertiary structure
as hydrophilic R groups are on the outside
Haemoglobin is a conjugated
quaternary protein comprising:
two alpha-globulins
two beta-globulins
four haem groups, each with
an iron atom at its core
It is the iron atoms which bind
oxygen.
Hb + 4O2
loading
unloading
HbO8
Keratin in hair
A hair