Linoleic
linolenic
Minyak
Kelapa
Minyak
Kelapa
Minyak Inti
Sawit
(%)a
Sawit (%)b
(%)b
Asam Kaprat
4,9 9,5
Asam Kaprilat
5,5 9,5
34
Asam Kaproat
0,0 0,8
37
Asam Laurat
44,0 52,0
46 52
Asam Miristat
13,0 19,0
1,1 2,5
14 17
Asam Palmitat
7,5 10,5
40 46
6,5 9
Asam Palmitoleat
0,0 13,1
Asam Stearat
1,0 3,0
3,6 4,7
1 2,5
Asam Oleat
5,0 8,0
39 45
13 19
Asam Linoleat
1,5 2,5
7 11
0,5 - 2
Asam Arakhidonat
0,0 0,4
DEFINISI
Pangan tradisional BERBAHAN LOKAL:
Bahan baku atau resep makanan dan minuman yang terbuat dari bahan-bahan
yang terdapat di Indonesia dan telah dikenal sejak dahulu.
Contoh:
tempe, tahu, oncom, bir pletok, wedang jahe, cincau,
tape beras, peujeum, dodol, kredok, urap, asinan,
sayur asin, kacang rebus, tauge sayuran dan buahbuahan tropis, bahan lalapan, rumput laut, dsbnya
Pangan fungsional
Makanan atau minuman yang berpengaruh positif terhadap kesehatan,
Kegiatan fisik dan mental, disamping kandungan zat-zat gizinya
Pangan tradisional
Nutraceuticals
Fungsional/
Biologis
Fungsional:
Fungsi biologis seluler: sintesis protein, enzim, hormon,
DNA, RNA, senyawa metabolik
Kesehatan:
Tanggung jawab bersama
Ilmu dan teknologi pangan dengan farmakologi
dan kedokteran
Paradigma sehat
SAKIT MENJADI
SEMBUH
OBAT
SEHAT
=
TIDAK SAKIT
TETAP TIDAK
MAKANAN
(FUNGSIONAL)
Pangan/bahan alami:
segar, .olahan
Metabolisme
(pembongkaran)
Cells dlm
organ tubuh
PARADIGMA
SEHAT
WHO 2002
SEHAT
USAHA
PENCEGAHAN:
Pencegahan
PANGAN,
NATURAL PRODUCTS
Perawatan
SEHAT
USAHA
KEDOKTERAN
FARMASI
Pengobatan
SAKIT
Pangan/makanan/bahan
nabati/natural products yang dapat
mencegah kanker dapat mencegah
penyakit jantung,
Klorofil
serat
Daun cincau
Antosianin
Flavonoid/fenolik
Isotiosianat
Karotenoid,
terpenpoid
Bagaimana
terjadinya kanker ?
Gen
Keturunan:
15%i
Gen
Gen
Polusi udara,
pencemaran
makanan,
Uvsinar
matahari,,
virus,
infeks:
85%
Gen
Gen
Gen
Gen
Gen
Polusi udara, pencemaran makanan,
UV/sinar matahari,,kegemukan, hormon
Gen
Gen
Gen
METASTASE
MENYEBAR
Gen
Gen
pembetul
Normal DNA
Gen
Gen
Kerusakan
gen lanjut
Gen
Diperngaruhi oleh:
Zat
gizi
Diperngaruhi oleh:
Aktivitas fisik,
kegemukan,
makanan
yang dikonsumsi
Hormon dan
faktor
pertumbuhan
tertentu spt
karotenoid,
retinol
Serat, bakteri
Gen
kolon, asam lemak
yang mudah
Sel normal
menguap,
pre/probiotik
Gen
Sel tidak normal
Gen
Sel bunuh diri
Pangan
Zat gizi:
protein,lemak,
karbohidrat, vitamin,
mineral, serat
Tanaman obat
Sumber utama
----------------
Komponen
fungsional/bioaktif
Non-gizi
Kadar rendah
Penting
Kemungkinan toksik
Sangat kecil
Kadar tinggi
tidak penting
Besar
Jenis
Fungsi
Sumber
Protein, lemak,
karbihidrat
Zat pembangun,
Energi, pelindung
Esensial
Biji-bijian,
Kacangkacangan,
Daging, ikan,
dll
Vitamin dan
Mineral esensial
A, B, C, D, E, K,
Folat, pantotenat,
Niasin, biotin
Metabolisme
Seluler normal
Esensial
Sayuran dan
buah-buahan,
Rumput laut,
sintetik
Serat
Selulosa, pektin,
hemiselulosa
Gum, oligoskarida
Prebiotik,
Kontrol kolesterol,
Pencernaan,ImunitasDia
betes, kanker,
kegemukan
Sayuran dan
buah-buahan,
Rumput laut
Sintetik
Gula alkohol
Asam amino,
Jenis
Fungsi
Sumber
Eritritol
Arabitol, ribitol,
xilitol
Sorbitol,
manitol,
Ganggang, jamur,
Exudat tanaman,
molases, rumput
laut
Arginin
Aspartat/gin
Antihipertensi
Fatig kronik, sirosis hati
Anti epilepsis
Anti insomnia
Analgesik
Anti depresi/ Parkinsons
diseases
Anti depresi/hiperaktif
Protein
Glutamat
Triptofan
Tirosin
Fenilalanin
Jenis
Fungsi
Sumber
Peptida
Casomorphin
Imunopeptida
Caseinophosphopepti
da
Peptida bioaktif
Anti diarea
Stimulasi imunitas
Absorpsi Ca
Antihipertensi
Kasein susu
Bakteri asam
laktat
Lactococcus,
Lactobacillus,
Bifidobacterium,dll
Lactose intolerant
Probiotik: diarea, anti
kolesterol/ kanker/
konstipasi
imunostimulan,
Produk fermentasi
Susu, sayuran, dll
PUFA, -6,
Metabolisme
arakhidonat, anti
penyakit kronis
Lemak tanamn
Daun, biji-bijian,
ikan.
-3
Hidrolisa protein
kacang-kacangan,
ikan
Jenis
Fungsi
Sumber
Thioallyl
CH2=CH-CH2X
X=struktur
organik
Hypolipidemic
Antitrombotik
Anti kanker
Bawang putih
Protese inhibitor
Kunitz
Anti kanker
Kedele, kacangkacangan
Chlorophyllins
Khlorofol
tanaman
Antikanker
Khlorofol
tanaman
Lignans
Antikanker
Estrogen
Kedle, gandum
PEITC
(Phenethyl
isothiocyanate)
Antikanker
Cruciferous
Antikanker
Kunyit
Curcumin I, III
Diferuroilmetan
Fungsi
Sumber
Karotenoid
Gingerols,
shogaol
Antioxidan, anti
ateroskelosis,
Pencernaan, Anti kanker
Jahe
Ubiquinone
s,
ubiquinols
Antioxidan
Imunomodulator (AIDS)
Minyak jagung,
kacang-kacangan
Tanaman
Flavonoids
Fenol
sederhana
Jenis
Quercetin,
galangin,Ruti
n, diosmin
katekin
Teh
Tanaman
Jenis
Fungsi
Sumber
Isotiosianat
sulfofran
antikanker
brokoli
Actoxikavikol
asetat
Fenil propanoid
Anti kanker
Languas galanga
Aurapten (AURA)
D-limonen
Anti kanker
Sitrus
Resveratrol
Trihidroxistilben
Anti kanker
Anggur merah
Laktoferin
Protein
Anti kanker
Susu
Fitosterol
B-sitosterol,
kampesterol
Anti kanker
Hipokholesterol
Sayruan, bijibijian
Saponin
Glikosida
Anti kanker
Kedele
Antioxidan, Anti
kanker
Kedele, sorgum,
Momordisin
Anti kanker
paria
Cucurbitasin
Anti cacing
Labu
Curcuma xanthoriza
Zingiber officinale Roxb
Languas galanga (L) Stuntz
Kaempferia galanga L
Eugenia aromatica L. O.K.
Curcuma domestica Val
Zingiber purpureum Roxb
Momordica charantina L
1554351
1364270
1256148
615418
556500
488686
215432
140427
528845
872642
Bahan baku:prinsipal/suplemen:
sayuran buah-buahan, rempah-rempah, ganggang,
rumput laut, mikroorganisme, jamur
INTRODUCTION
GINGER
Zingiber family
The rhizome is commonly accepted as a source of
flavor :
cooking, drinks, baking
Grows well in tropical rainforest climate
Traditional beliefs:
prevention of common cold, physiological and
stomach disorders,
inflammation, diarrhea, etc (Tang and Eisenbrand,
1992)
Scientific Reports
Antioxidant capacity of ginger extract (oleoresin
fractions) in
linoleic acid system > a-tocopherol (Kikuzaki and
Nakatani,1993)
Oleoresin fractions
gingerol, shogaol
Modified LDL
( Ox LDL, Ac LDL)
Scavenger Reseptor
Phagocytosis
Endosome
UC
EC
HDL
Pinocitosis
LDL
UC
Translocation
LDL
Reseptor
Lysosome
Cholesterol up
take
receptor
synthesis
Acetate
Cholesterol
Cholesterol
processing
Cholesterol
exclussion
Lipid streaks
Monocyte
LDL
Adhesion
entry
Lipid
oxidation
Foam cells
differentiation
ROS
Protein
modification
Uptake LDL
Macrophage
RESEARCH OBJECTIVES
METHODOLGY
Subjects: 24 healthy male students living in the same religious dormitory
two groups: treated group and control/placebo group
Ginger drinks:
water extract, pasteurized 850C, added with sugar syrup
standardized acceptable drink
given every afternoon at 17:00
placebo group received syrup without ginger extract
Plasma analysis:
1. Total cholesterol (CHOD-PAP method, Boehringer-Mannheim, kit)
2. LDL-C (PVS method, Boehringer-Mannheim, kit)
3. HDL-C (phosphotungstic acid)
LDL isolation:
8 ml plasma + 5 ml NaCL 0.9% + EDTA 0.01%
incubation in polyalomer tubes
Ultracentrifugation at 36.000 rpm 40C, 20 hours
Tube cutting
VLDL
LDL + LDL (d> 1.006 g/ml)
Scaled tubes + 0.1109 g KBr/ml
Mixing
Transfer to new polyaomer tubes + 4 ml KBr in NaCl-EDTA
Ultracentrifuse, 36.000 40C 24 hrs
LDL oxidation:
The isolated LDL was oxidized with 5 M CuSO4 at 370C
conjugated dienes was monitor continuously at 234 nm for 30 minutes
MDA LDL analysis:
Extraction with butanol
measurement at 515 nm exitation, 553 nm emission (Conti et al
1991)
RESULTS
180
160
140
m g /d l
120
HDL-c
LDL-c
100
Total-c
80
TG
60
40
TG
20
Total-c
0
LDL-c
Bef ore
TREATMENT
After
HDL-c
Before
P LACEBO
Af ter
Figure 1. HDL, LDL, and total cholesterol, and triglyceride in plasma of male student
subjects before and after treatment with ginger drink for 30 days (n=12) and
those in control placebo subjects (n=12)
800
700
600
0 time of control
500
30 time of control
400
0 time of treatment
300
30 time of treatment
200
100
0
0
25
50
75
100
125
Time, m in
500
Free Cholesterol
450
400
Ester kolesterol
350
Total cholesterol
300
250
200
150
100
50
0
Control
Dichloromethane
extract
a-Tocopherol
CONCLUSSION
1.
2.
3.
Table. Average and range values of lymphocyte cell analysis of proliferation activities (SI) in
the presence of Con A, LPS or paraquat; of MDA cell; and of percentage of CD3 cells
No
Parameters
Subjects
SI of cells
cultured with
Con A
Treated group
SI of cells
cultured with
LPS
SI of cells
cultured with by
paraquat
MDA cells
(mol/l)
Percentage of
CD3+
cells
Average values
Day 0
Day 30
3.33
(2.63-6.40)
7.87
(4.58-10.75)
0.63
(0.46-0.75)
10.42
(4.63-16.75)
Treated group
1.39
(1.09-1.80)
2.07
(0.83-3.34)
Control group
1.74
(0.98-3.06)
2.05
(0.64-6.08)
Treated group
1.15
(0.75-1.83)
2.15
(0.83-5.44)
Control group
1.28
(0.79-2.32)
1.62
(0.62-3.94)
Treated group
3.33
(2.63-6.40)
0.63
(0.46-0.75)
Control group
3.03
(2.55-4.40)
0.83
(0.67-1.06)
Treated group
82.42
(77.74-91.29)
80.19
68.15-91-99)
Control group
79.52
(63.75-93.15)
78.44
(69.38-87-62)
Control group
35
30
25
20
15
10
5
0
100;1
50;1
Control group
(n=12)
Treated group
(n=12)
60
50
40
30
20
10
0
100;1
50;1
Control group
(n=12)
Treated group
(n=12)
Figure 1.Increasing NK lysing activity of 12 healthy male subjects given ginger drink..Blood was
withdrawn for lymphocyte isolation at before treatment (A) and after treatment (B) from control
and treated groups. Ginger drink was given for 30 days every afternoon and the cells were
incubated with target cells which had been treated with H3-thymidine with the ratio of 100:1 or
50:1. Data was expressed as count per minute of the unlysed target cells.
25
20
15
10
5
0
0
30
MDA of
treated
subjects
(umol/L)
MDA of
control
subjects
(umol/L)
Vitamin E of
treated
subjects
(umol/L)
Vitamin E of
control
subjects
(umol/L)
Figure 2. MDA and vitamin E content in plasma of subjects before (0 day) and after receiving ginger
drink for 30 days (n=12). The subjects were healthy male students living in the same dormitory. MDA
was analyzed using the method of Conti et al (1991).Vitamin E was analyzed using spectrofluorometer.
Figure.
CONCLUSSION
In human study, using male adult healthy subjects, there is improvement in T cells
proliferation activities and percentage.
Improvement on T cells was accompanied by resistance of lymphocyte cells against
oxidative stress. This improvement was demonstrated by the cells obtained from the
subjects drinking ginger
Resistance of the lymphocytes was not accompanied by decrease in MDA cell
Beside the role of ginger compounds as protectant against oxidative stress, they appeared
to have other immunoenhancement activity, notably in improving NK cell activity. This
improvement in NK cell activity had been observed in the male student studied receiving
ginger drink for 30 days
analysis of ginger substances in the plasma reveal the presence of the assumed gingerol
group in the plasma of subjects drinking ginger, higher than that of the control group.
Since NK cells are known to have specific activity in lysing mutated or infected cells,
the results of this research has shown the scientific support of the traditional beliefs that
ginger can improve body resistance to common cold.
LITERATURE REVIEW
1. Tang W and Eisenbrand G. 1992. Chinese Drugs of Plant Origin: Chemistry, Pharmacology and Use in Traditional
and Modern Medicine. Springer-Verlag, New York.
2. Nakatani N. 1997. Natural antioxidants from spices. In: Huang M, Ho C, Lee CY (Eds). Phenolic Compounds in
Food and Their Effects on health II. Am Chem Soc., Washington DC.
3. Hikino H, Kiso Y, Kato N, Hamada Y, Shioiri T, Aiyama R, Itokawa H, Kiuchi F and Sankawa U. 1985.
Antihepatotoxic actions of gingerols and diarylheptanoids. J Ethnopharmacol 14: 31-39
4. Kikuzaki H. and Nakatani N. 1993. Antioxidant effects of some ginger constituents. J Food Sci, 58: 1407-1410
5. Zakaria-Rungkat, F, Darsana L and Wijaya H. 1996.Immunity enhancement and cell protection activity of ginger
buds and fresh ginger on mouse spleen lymphocytes. Symp Non-Nutritive Health Factors for Future Foods.
Korean Soc. Food Sci and Technol, Korea
6. Antipenko, AY, Spielman AI, and Kirchberger MA.1999. Interactions of 6-Gingerol and Ellagic Acid with the
Cardiac Sarcoplasmic Reticulum Ca2+-ATPase. J Pharmacol Exp Therapeutics, 290, 227-234
7. Conti M, Morand PC, Levillain P, and Lemonnier A. 1991. Improved fluorometric determination of
malonaldehyde. J Clin Chem, 37/7: 1273-1275
8. Fritz KL, Nelson TL, Ruiz-Velasco V, and Mercurio SD. 1994. Acute intramuscular injection of oils or oleic acid
component protects mice against paraquat lethality. J Nutr. 194: 425-42
9. Meydani SN, Wu D. Santos MS and Hayek MG. 1995. Antioxidants and immune response in aged persons:
Overview of present evidence. Am J Clin Nutr. 62: 146S-147S
10. Zakaria-Rungkat, F., Nurahman, Prangdimurti, E., Tejasari. 2003. Antioxidant and Immunoenhancement
Activities of Ginger (Zingiber officinale Roscoe) Extracts and Compounds in In Vitro and In Vivo Mouse
and Human System. Nutraceuticals and Foods.8; 96-104
ACKNOWLEDGEMENT
This reseach was funded by the University Research Graduate Education)-Project III 97-2000, Department of Higher
Education, Ministry of National Education
HEALTHY DESSERT
MAKANAN PENCUCI MULUT SEHAT
MAKANAN PENCUCI MULUT FUNGSIONAL
PANGAN (PENCUCI MULUT) FUNGSIONAL
AKTIFITAS
ANTI KANKER GEL CINCAU HIJAU
(Cyclea barbata L.Miers)
Fransiska Rungkat-Zakaria,
Endang Prangdimurti, Edna Ananta, Albertus Seno Pandoyo
PENDAHULUAN
Secara tradisional, tanaman cincau hijau (Cyclea barbata L. Miers)
digunakan sebagai obat penurun panas, obat radang lambung, mual, dan
penurun tekanan darah tinggi. Minuman cincau merupakan produk olahan yang
berbentuk gel dan dibuat dari daun cincau hijau melalui proses extraksi dingin.
Beberapa laporan hasil penelitian menunjukkan bahwa tanaman cincau
mempunyai aktivitas sitotoksik dan antimalaria (Guinaudeau, 1993). Aktivitas
sitotoksik ini dihipotesiskan dapat menghambat proliferasi sel kanker atau sel
tumor di dalam tubuh..
METODA PENELITIAN
Extraksi air daun segar dengan aquades dilakukan secara manual karena
terbentuk gel yang kemudian didinginkan untuk memperoleh cairan sineresis.
Extrak air daun, akar dan batang dikeringbekukan.
Extrak etanol dan hexan dilakukan pada daun, akar dan batang keringbeku.
Extrak di tambahkan pada media kultur sel-sel kanker K562 (turunan sel
leukimia) dan Hela (turunan sel kanker servix) dan pada kultur sel limfosit
yang diisolasi dari darah tepi seorang mahasiswa sehat, dengan 5 tingkat
konsentrasi: C1 (1/2 C2); C2 (setara dengan konsumsi segelas minuman
cincau), C3 (2xC2); C4 (4xC2); C5 (8xC2).
% Penghambatan
100
50
0
c1
c2
c3
-50
c4
c5
Aid
etd
hed
aib
etb
heb
aia
eta
hea
Konsentrasi
Gambar 1. Efek sitotoksik extrak air (ai), etanol (et) dan hexan
(he) dari daun (d), batang (b) dan akar (a) tanaman cincau hijau
terhadap pertumbuhan sel leukemia (K562). Extrak air akar
mempunyai persentase penghambatan diatas 61%, air batang
diatas 30%, sedang air daun diatas 37% setelah konsentrasi diatas
C3. Peningkatan konsentrasi > C3 (2x 2 gelas muniman gel
cincau) tidak nyata menaikkan aktifitas sitotoksik.
50
% Penghambatan
40
30
20
10
0
-10
c1
c2
c3
c4
c5
Aid
etd
hed
aib
etb
heb
aia
eta
hea
-20
-30
Konsentrasi
Gambar 2. Aktifitas sitotoksik extrak air (ai), etanol (et) dan hexan
(he) dari daun (d), batang (b) dan akar (a) tanaman cincau hijau
terhadap kehidupan sel kanker servix (Hela).Ekstrak Aia hingga
konsentrasi C4 (setara 4 gelas minuman gel cincau) menghambat
pertumbuhan sebesar 31%, extrak aid 22% pada konsentrasi C4.
Tabel 1. Aktifitas sitotoksik (persen) extrak gel cincau pada sel limfosit manusia
yang dikultur dalam media RPMI 1640 lengkap. Sel hidup dianalisa dengan MTT
Sampel
Aid
C1
C2
C3
C4
C5
-9
-2
-17
-18
-1
Etd
-10
-26 *
Hed
49 *
16
31*
38 *
Aib
-11
-19 *
-39 *
-3
Etb
-12
-2
-7
-25 *
14
Heb
-22 *
-11
-2
-7
Aia
-1
-17
-38 *
-52 *
Eta
-9
11
-26 *
10
Hea
31
-14
-84
(*) menunjukkan perbedaan nyata terhadap kontrol pada selang kepercayaan 95%
Bilangan negatif menunjukkan penurunan nilai absorbansi.
Bata
ng
Akar
Pelar
ut
Air
Etanol
Hexan
Air
*) Scopolamin
% b.b % b.k
0.12
0.37
0.69
1.84
*)
Tropic acid
% b.b
% b.k
1.28
3.85
0.16
0.49
-
Etanol
0.44
1.18
Hexan
Air
Etanol
Hexan
0.07
0.30
-
0.18
0.61
-
1.23
0.59
2.52
1.20
KESIMPULAN
1
2
3
4
5
1,2)
3)
INTRODUCTION
Tumour growth:
oxidative stress, immunological disorder, malnutrition
Potential role for oxidative-induced injury in the cancer process
specifically during the promotion stage (Klaunig et al, 1998)
Sources of ROS :
from inflammatory cells (Cerutti and Trump, 1991)
A need in research to find suitable food for cancer patients
Green gel leaf Cyclea barbata L.Miers
common refreshing drink in South East Asian countries
known traditionally to have cooling and anti
inflammation effects in the body
Tumor transplantation
Tumor latent
period (days)
Killed , Day 57
Liver
Tumor volume
(Moving pen,
Tajima)
Tumor
Healthy and necrotic
Spleen
Spleen cells
Tumor cells
Resistance to
H2O2 (MTT)
T cell
(Rosette)
LIVER
SOD
(Adenochrome asay
Misra. H.P.
GSH-PX
(Paglia and Valentine,
1967
CATALASE
Colometri, Sinha 1972
MDA
(TBA, Buege and
Aust)
Total Glutathion
DTNB, Ellman. 1982
3.5
3
2.5
2
1.5
1
0.5
0
LP + T
SC
2.5
2
Cm
1.5
1
0.5
0
LP + T
SC
4
G
r
a
m
s
2
0
LP + T
Necrose
T
SC
Whole tumor
50
40
% necrotic 30
tissue
20
10
0
LP + T
SC
T Cell, %
16,2
8,8
7,5
CP
SC
77
69,3
80
D
e
a
d
t
u
m
o
r
c
e
l
l
s
,
59,3
70
60
50
40
30
20
% 10
0
SC
LP+T
Absorbancy, 570 nm
4,50
4,00
3,50
3,00
2,50
2,00
1,50
1,00
0,50
0,00
Without H2)2
NC, 3W
With H2O2
NC, 4W
LP, 3W
LP, 4W
MDA
SOD
15
500
400
300
200
100
0
10
5
0
LP + T
SC
LP + T
SC
600
400
200
0
Catalase
GSH-PX
LP + T
GSH
SC
CONCLUSION
1. There is no difference in the latent period of the tumor growth in
mice. The leaf powder decrease tumor volume and tumor mass but
increase tumor necrotic tissue up to 3 times
2. Feeding leaf powder increased Tcell inspite of the tumor growth.
Slight increase in cytotoxicity in T and LP+T, indicate T cell
recognition of the tumor antigen
Prolonged tumor growth from 3 to 4 weeks markedly decrease
cells resistance to oxidative stress
3. The leaf powder increase SOD activity in mice but reduce
MDA in the liver Reduce liver catalase activity but did not
effect the other enzyme activity in the liver.
Conclusion
Potensi komoditi lokal sangat besar untuk
produk pangan fungsional
Bahan baku pangan tradisional mengandung
komponen fungsional/bioaktif
Ketersediaan bahan berlimpah
Potensi untuk suplemen dari bahan baku pangan,
rempah-rempah, tanaman obat
Rekayasa pangan fungsional dari bahan/resep
tradisional
Potensi menu diet tradisional
Masih banyak komoditi yang harus diteliti
Thank
Administration Building
Bogor agricultural University
Indonesia
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